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The map label for this gene is hyaB [H]

Identifier: 238921265

GI number: 238921265

Start: 3293198

End: 3294991

Strand: Reverse

Name: hyaB [H]

Synonym: NT01EI_3408

Alternate gene names: 238921265

Gene position: 3294991-3293198 (Counterclockwise)

Preceding gene: 238921266

Following gene: 238921264

Centisome position: 86.43

GC content: 62.1

Gene sequence:

>1794_bases
ATGAGTTACCAGTATCAAACCCAAGGATATACCTTAAGCGATGCCGGCCGTCGCCTGGTGGTGGACCCGATTACGCGCAT
TGAAGGTCACATGCGCTGCGAAGTGAACATCAACGACCAGAACATCATTACCAACGCCGTCTCCTGCGGCACCATGTTCC
GCGGACTGGAGATCATTCTGCAGGGGCGTGACCCGCGCGACGCCTGGGCGTTCGTGGAGCGTATCTGTGGGGTATGCACC
GGGGTGCACGCCCTGGCCTCGGTGTACGCCATCGAGGATGCCATCGGCATCAAGGTGCCGGACAACGCCAATATCATCCG
TAACATCATGTTGGCCACCCTGTGGTGCCACGACCATCTGGTGCACTTTTATCAGCTGGCGGGGATGGACTGGATCGACG
TGCTGGATGCCCTGAAGGCCGATCCGCGCGCCACCTCGCAGCTGGCGCAGAGTCTCTCCTCCTGGCCGATGTCGTCGCCT
GGCTACTTCTTTGACGTGCAAAACCGCCTGAAAAAGTTCGTGGAGGGCGGCCAGCTGGGGATCTTCCGCAACGGCTACTG
GGGGCACCCGCAGTACAAGCTGTCGCCGGAGGCCAATCTGATGGGCTTTGCCCACTATCTGGAGGCCCTCGACTTCCAGC
GGGAGATCGTCAAGATCCATACGGTGTTCGGCGGTAAGAACCCGCACCCGAACTGGATCGTCGGCGGCATGCCCTGTGCC
ATCAACATCGATCAGAGCGGCGCGGTCGGCGCGGTCAACATGGAGCGTCTCAACCTGGTGCAGTCGATCATTACCCGCAC
CGCCGACTTTATCAACAACGTGATGATCCCCGATGCGCTGGCCATCGGCCTGTTTAACAAGCCGTGGAGCCAGATCGGCA
CCGGTCTGTCCGACAAGTGCGTGCTGAGCTATGGCGCCTTCCCGGACATCGCCAACGACTTCAGCCAGAAGAGTCTGCTG
ATGCCGGGCGGTGCGGTGATCAACGGTGACTTCGCCAACGTGCTGCCGGTGGATCTGGCCGATCCGCAGCAGGTGCAGGA
GTTCGTCGACCACTCCTGGTATCGCTACCCCAACGGGCAGCAGGGGCGTCATCCGTTCGATGGCATCACCGACCCTTGGT
ACAACCCGGGCAACGTGAAGGGCAGCGCCACCGATATCCAGCAGCTGGACGAGCAGGCGCAGTACTCCTGGATCAAGGCG
CCGCGCTGGCGCGGTCATCCGATGGAGGTCGGCCCGCTGGCCCGTACCCTGATCGCCTATCACAAGGGCGATGCGGCGAC
CATCGAGTCGGTGGATCGCATGATGTCATCGCTCAAGCTGCCGCTCTCTGGCATTCAGTCTACCCTGGGCCGTATTCTGT
GCCGCGCCCACGAGGCGCAGTGGGCCGCCGGTAAGCTGCAGTACTTCTTCGACAAGCTGATGACCAACCTGAAGAACGGC
AATCTGGCGACGGCCAATACCGAGAAGTGGGAACCGGCCACCTGGCCGCAGCACTGCCGCGGTATCGGCTTTACCGAGGC
GCCGCGCGGTGCGCTGGGCCACTGGGCATCGATCCGCGATCAGAAGATCGATCTCTATCAGTGCGTGGTACCGACCACTT
GGAACGCCAGTCCGCGCGATCCCAAGGGACAGATCGGGGCCTATGAGGCGGCGCTGATGGGGACCCAGATGGCGATCCCC
GATCAGCCGCTGGAGATCCTGCGCACCCTGCACAGTTTCGACCCCTGCCTGGCCTGTTCAACCCACGTGCTCGGCGATGA
CGGCAGCGAATTGATCTCCGTCCAGGTACGCTAA

Upstream 100 bases:

>100_bases
GGGTACATGCGGTGGCCAGCGCTGTCAATCAGCGCAAACGCCACAACCAGCAGCTGACACAGGCAGAACAACAACAGCCG
GGCAATGAGGATAAACAGGC

Downstream 100 bases:

>100_bases
GCACTAAGGAGAAGAACGATGAGTGAAAAGACATCCCCGCACGCCGAGGAGGCGCGTGATGATGCCGTCAGCCACTATGT
GTTCGAGGCGCCGGTGCGCC

Product: hydrogenase 1 large subunit

Products: NA

Alternate protein names: HYD1; Membrane-bound hydrogenase 1 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MSYQYQTQGYTLSDAGRRLVVDPITRIEGHMRCEVNINDQNIITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCT
GVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRATSQLAQSLSSWPMSSP
GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTVFGGKNPHPNWIVGGMPCA
INIDQSGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGLFNKPWSQIGTGLSDKCVLSYGAFPDIANDFSQKSLL
MPGGAVINGDFANVLPVDLADPQQVQEFVDHSWYRYPNGQQGRHPFDGITDPWYNPGNVKGSATDIQQLDEQAQYSWIKA
PRWRGHPMEVGPLARTLIAYHKGDAATIESVDRMMSSLKLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNG
NLATANTEKWEPATWPQHCRGIGFTEAPRGALGHWASIRDQKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMGTQMAIP
DQPLEILRTLHSFDPCLACSTHVLGDDGSELISVQVR

Sequences:

>Translated_597_residues
MSYQYQTQGYTLSDAGRRLVVDPITRIEGHMRCEVNINDQNIITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCT
GVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRATSQLAQSLSSWPMSSP
GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTVFGGKNPHPNWIVGGMPCA
INIDQSGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGLFNKPWSQIGTGLSDKCVLSYGAFPDIANDFSQKSLL
MPGGAVINGDFANVLPVDLADPQQVQEFVDHSWYRYPNGQQGRHPFDGITDPWYNPGNVKGSATDIQQLDEQAQYSWIKA
PRWRGHPMEVGPLARTLIAYHKGDAATIESVDRMMSSLKLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNG
NLATANTEKWEPATWPQHCRGIGFTEAPRGALGHWASIRDQKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMGTQMAIP
DQPLEILRTLHSFDPCLACSTHVLGDDGSELISVQVR
>Mature_596_residues
SYQYQTQGYTLSDAGRRLVVDPITRIEGHMRCEVNINDQNIITNAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTG
VHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRATSQLAQSLSSWPMSSPG
YFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTVFGGKNPHPNWIVGGMPCAI
NIDQSGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGLFNKPWSQIGTGLSDKCVLSYGAFPDIANDFSQKSLLM
PGGAVINGDFANVLPVDLADPQQVQEFVDHSWYRYPNGQQGRHPFDGITDPWYNPGNVKGSATDIQQLDEQAQYSWIKAP
RWRGHPMEVGPLARTLIAYHKGDAATIESVDRMMSSLKLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNGN
LATANTEKWEPATWPQHCRGIGFTEAPRGALGHWASIRDQKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMGTQMAIPD
QPLEILRTLHSFDPCLACSTHVLGDDGSELISVQVR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=597, Percent_Identity=93.1323283082077, Blast_Score=1174, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=591, Percent_Identity=41.9627749576988, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66252; Mature: 66121

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQYQTQGYTLSDAGRRLVVDPITRIEGHMRCEVNINDQNIITNAVSCGTMFRGLEIIL
CCCEECCCCEEECCCCCEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCHHHEE
QGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHH
VHFYQLAGMDWIDVLDALKADPRATSQLAQSLSSWPMSSPGYFFDVQNRLKKFVEGGQLG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCEE
IFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTVFGGKNPHPNWIVGGMPCA
EEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEHEECCCCCCCCEEECCCCEE
INIDQSGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGLFNKPWSQIGTGLSDKC
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHCCCCCCCE
VLSYGAFPDIANDFSQKSLLMPGGAVINGDFANVLPVDLADPQQVQEFVDHSWYRYPNGQ
EEECCCCCHHHHHHHHCCEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHCCCEECCCCC
QGRHPFDGITDPWYNPGNVKGSATDIQQLDEQAQYSWIKAPRWRGHPMEVGPLARTLIAY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
HKGDAATIESVDRMMSSLKLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NLATANTEKWEPATWPQHCRGIGFTEAPRGALGHWASIRDQKIDLYQCVVPTTWNASPRD
CEEECCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
PKGQIGAYEAALMGTQMAIPDQPLEILRTLHSFDPCLACSTHVLGDDGSELISVQVR
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHCCCEECCCCCEEEEEEEC
>Mature Secondary Structure 
SYQYQTQGYTLSDAGRRLVVDPITRIEGHMRCEVNINDQNIITNAVSCGTMFRGLEIIL
CCEECCCCEEECCCCCEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCHHHEE
QGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHH
VHFYQLAGMDWIDVLDALKADPRATSQLAQSLSSWPMSSPGYFFDVQNRLKKFVEGGQLG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCEE
IFRNGYWGHPQYKLSPEANLMGFAHYLEALDFQREIVKIHTVFGGKNPHPNWIVGGMPCA
EEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEHEECCCCCCCCEEECCCCEE
INIDQSGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGLFNKPWSQIGTGLSDKC
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHCCCCCCCE
VLSYGAFPDIANDFSQKSLLMPGGAVINGDFANVLPVDLADPQQVQEFVDHSWYRYPNGQ
EEECCCCCHHHHHHHHCCEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHCCCEECCCCC
QGRHPFDGITDPWYNPGNVKGSATDIQQLDEQAQYSWIKAPRWRGHPMEVGPLARTLIAY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
HKGDAATIESVDRMMSSLKLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NLATANTEKWEPATWPQHCRGIGFTEAPRGALGHWASIRDQKIDLYQCVVPTTWNASPRD
CEEECCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
PKGQIGAYEAALMGTQMAIPDQPLEILRTLHSFDPCLACSTHVLGDDGSELISVQVR
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHCCCEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA