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The map label for this gene is hyaA [H]

Identifier: 238921266

GI number: 238921266

Start: 3294988

End: 3296109

Strand: Reverse

Name: hyaA [H]

Synonym: NT01EI_3409

Alternate gene names: 238921266

Gene position: 3296109-3294988 (Counterclockwise)

Preceding gene: 238921267

Following gene: 238921265

Centisome position: 86.46

GC content: 61.23

Gene sequence:

>1122_bases
ATGAATAACGAGGATACCTTTTACCAAGCCATGCGCCGTCAGGGCGTGACCCGGCGGAGTTTTCTCAAGTATTGCAGTCT
GGCTGCCACGTCGCTGGGGCTGGGTGCCGGGATGGCGCCAAAGATAGCCTGGGCGCTGGAGAATAAACCGCGTATTCCGG
TCGTCTGGATCCACGGCCTGGAGTGTACCTGCTGTACCGAATCCTTTATCCGCTCCTCACACCCGCTGGCCAAGGATGTG
ATCCTCTCGCTCATCTCCCTCGATTATGACGATACCCTGATGGCGGCCGCCGGAACGCAGGCCGAGGCGGTATTTGAGGA
CATCATCACCCGCTACCACGGCAAGTACATTCTGGCGGTGGAGGGGAATCCGCCGCTGGGTGAGCAGGGGATGTTCTGTA
TCAGCAGCGGTCGTCCGTTTATTGAAAAATTGAAAAAAGCCGCCGCCGGTGCCAGCGCCATCATCGCCTGGGGGACCTGT
GCCTCCTGGGGCTGCGTGCAGGCCGCCCGTCCCAATCCGACCCAGGCCACCCCCATCGACAAGGTGATCACCGACAAGCC
GATCATCAAGGTGCCCGGCTGTCCGCCGATCCCGGACGTGATGAGCGCCATCATTACCTATATGGTGACCTTTGACCGTC
TGCCGGACGTTGACCGCATGGGGCGTCCGCTGATGTTCTACGGTCAGCGTATCCACGACAAGTGTTACCGCCGCGCCCAC
TTCGACGCCGGTGAGTTCGTCCAGAGCTGGGATGACGATGCCGCCCGTAAGGGGTACTGCCTGTACAAGATGGGCTGTAA
GGGGCCAACCACCTATAACGCCTGCTCTTCCACCCGCTGGAACGACGGCGTCTCTTTCCCGATCCAGTCCGGCCACGGCT
GTCTGGGCTGCTCAGAGAATGGCTTCTGGGATCGCGGCTCGTTCTACAGCCGGGTGGTCGATATTCCGCAGATGGGGACC
CACTCCACCGCCGATACCGTCGGCCTGACGGCGCTGGGCGTGGTTGCCGCGGGGGTCGGGGTACATGCGGTGGCCAGCGC
TGTCAATCAGCGCAAACGCCACAACCAGCAGCTGACACAGGCAGAACAACAACAGCCGGGCAATGAGGATAAACAGGCAT
GA

Upstream 100 bases:

>100_bases
CGCACGCGATCCGATGGCTTGCCTCGCTGGGGGGACGTCGCTGGCCGATCAAACGCGCCCGGCACACCCGCCGGTGCTGC
AAGGAGAAGAGACGTGCGTT

Downstream 100 bases:

>100_bases
GTTACCAGTATCAAACCCAAGGATATACCTTAAGCGATGCCGGCCGTCGCCTGGTGGTGGACCCGATTACGCGCATTGAA
GGTCACATGCGCTGCGAAGT

Product: hydrogenase-1 small chain (HYD1)

Products: NA

Alternate protein names: HYD1; Membrane-bound hydrogenase 1 small subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNNEDTFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAWALENKPRIPVVWIHGLECTCCTESFIRSSHPLAKDV
ILSLISLDYDDTLMAAAGTQAEAVFEDIITRYHGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKKAAAGASAIIAWGTC
ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAH
FDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCSENGFWDRGSFYSRVVDIPQMGT
HSTADTVGLTALGVVAAGVGVHAVASAVNQRKRHNQQLTQAEQQQPGNEDKQA

Sequences:

>Translated_373_residues
MNNEDTFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAWALENKPRIPVVWIHGLECTCCTESFIRSSHPLAKDV
ILSLISLDYDDTLMAAAGTQAEAVFEDIITRYHGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKKAAAGASAIIAWGTC
ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAH
FDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCSENGFWDRGSFYSRVVDIPQMGT
HSTADTVGLTALGVVAAGVGVHAVASAVNQRKRHNQQLTQAEQQQPGNEDKQA
>Mature_373_residues
MNNEDTFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAWALENKPRIPVVWIHGLECTCCTESFIRSSHPLAKDV
ILSLISLDYDDTLMAAAGTQAEAVFEDIITRYHGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKKAAAGASAIIAWGTC
ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAH
FDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCSENGFWDRGSFYSRVVDIPQMGT
HSTADTVGLTALGVVAAGVGVHAVASAVNQRKRHNQQLTQAEQQQPGNEDKQA

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=373, Percent_Identity=95.9785522788204, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1789371, Length=329, Percent_Identity=39.209726443769, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 40726; Mature: 40726

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNEDTFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAWALENKPRIPVVWIHGL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECC
ECTCCTESFIRSSHPLAKDVILSLISLDYDDTLMAAAGTQAEAVFEDIITRYHGKYILAV
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
EGNPPLGEQGMFCISSGRPFIEKLKKAAAGASAIIAWGTCASWGCVQAARPNPTQATPID
CCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
KVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAH
HHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHC
FDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCSEN
CCHHHHHHHCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCC
GFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGVVAAGVGVHAVASAVNQRKRHNQQLTQ
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEQQQPGNEDKQA
HHHHCCCCCCCCC
>Mature Secondary Structure
MNNEDTFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAWALENKPRIPVVWIHGL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECC
ECTCCTESFIRSSHPLAKDVILSLISLDYDDTLMAAAGTQAEAVFEDIITRYHGKYILAV
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
EGNPPLGEQGMFCISSGRPFIEKLKKAAAGASAIIAWGTCASWGCVQAARPNPTQATPID
CCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
KVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAH
HHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHC
FDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCSEN
CCHHHHHHHCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCC
GFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGVVAAGVGVHAVASAVNQRKRHNQQLTQ
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEQQQPGNEDKQA
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA