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The map label for this gene is pgk [H]

Identifier: 238921225

GI number: 238921225

Start: 3253986

End: 3255149

Strand: Reverse

Name: pgk [H]

Synonym: NT01EI_3368

Alternate gene names: 238921225

Gene position: 3255149-3253986 (Counterclockwise)

Preceding gene: 238921226

Following gene: 238921224

Centisome position: 85.39

GC content: 59.45

Gene sequence:

>1164_bases
ATGTCTGTAATTAAGATGACCGATTTGGATCTGGCGGGTAAACGCGTTCTGATCCGTGCGGATCTGAACGTTCCGGTTAA
AGACGGTAAAGTGACTTCCGATGCGCGTATCCGCGCTTCGCTGCCGACCATTGAGCTGGCGCTGAAGCAAGGTGCACGCG
TGATGGTAACCTCCCATCTGGGTCGTCCGACCGAAGGCGAGTACAACGCAGAGTTTTCTCTGCTGCCGGTGGTGAATTAC
CTCAAAGATCATCTGAAGGCGTCGGTGCGTCTGGCCAAAGATTACCTGGACGGCGTTGACGTCGCCCAGGGCGAGCTGGT
GGTGCTGGAAAACGTCCGCTTCAACAAGGGTGAGAAGAAAGACGACGAAACCCTGTCCAAGAAATACGCCGCCCTGTGCG
ACGTGTTCGTTATGGACGCGTTTGGCACCGCGCACCGCGCCCAGGCCTCTACCCACGGCGTGGGCAAGTTTGCGCCGATC
GCCTGTGCCGGTCCGCTGCTGTCCGGCGAGCTGGAAGCCCTGGGCAAGGCCCTGAGCAACCCGGCGCGTCCGATGGTCGC
CATCGTCGGCGGCTCCAAGGTCTCCACCAAACTGACCGTCCTGGACTCCCTGTCCAAGATCGCTGATCAGCTGATCGTGG
GCGGCGGCATCGCGAATACCTTCATCGCCGCGGAAGGCCATAACGTGGGTAAATCCCTGTATGAAGCCGACCTGATCCCT
GAAGCCAAGAAGCTGCTGGGTACCTGTGATATCCCGGTACCGACCGATGTGCGTGTGGCGACAGAGTTTTCTGAGACGGC
GTCGGCGACCATGAAATCCGTCAGCGAAATCAAAGATGATGAGCAGGTTCTGGATCTGGGCGATGCCTCTGCCGAGCAGC
TGGCCGCTATCCTGAAAAACGCCAAGACCATCCTGTGGAACGGTCCGGTCGGCGTTTTCGAGTTCCCGAACTTCCGTAAA
GGGACCGAGATCGTGGCCAATGCTATCGCCAACAGCGACGCCTTCTCCATCGCCGGCGGCGGCGATACCCTGGCGGCCAT
CGATCTGTTCGGCATCGCCGACAAGATCTCCTACATCTCCACCGGCGGCGGTGCCTTCCTGGAGTTTGTGGAAGGCAAGA
AACTGCCGGCTGTGGTGATGCTCGAAGAGCGCGCCAAGCAGTAA

Upstream 100 bases:

>100_bases
TACCACGCTGGCGATGGCCCGGGCAGTTTCTAATGCGTCGTAGCCGCGCGCGGCGACACAGCTTAAAGCAACTTTTAGAG
AATCAATGAGAGGATTCACC

Downstream 100 bases:

>100_bases
TGCCCGATACGGGAGGCCATCATGCCTCCCGTTGTTTTATCGCGCCTTGGCGCGGTAATGTTGATCCGATTGGAAAACAC
TCACGCCCACGAAACAGGAC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSVIKMTDLDLAGKRVLIRADLNVPVKDGKVTSDARIRASLPTIELALKQGARVMVTSHLGRPTEGEYNAEFSLLPVVNY
LKDHLKASVRLAKDYLDGVDVAQGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAPI
ACAGPLLSGELEALGKALSNPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHNVGKSLYEADLIP
EAKKLLGTCDIPVPTDVRVATEFSETASATMKSVSEIKDDEQVLDLGDASAEQLAAILKNAKTILWNGPVGVFEFPNFRK
GTEIVANAIANSDAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKKLPAVVMLEERAKQ

Sequences:

>Translated_387_residues
MSVIKMTDLDLAGKRVLIRADLNVPVKDGKVTSDARIRASLPTIELALKQGARVMVTSHLGRPTEGEYNAEFSLLPVVNY
LKDHLKASVRLAKDYLDGVDVAQGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAPI
ACAGPLLSGELEALGKALSNPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHNVGKSLYEADLIP
EAKKLLGTCDIPVPTDVRVATEFSETASATMKSVSEIKDDEQVLDLGDASAEQLAAILKNAKTILWNGPVGVFEFPNFRK
GTEIVANAIANSDAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKKLPAVVMLEERAKQ
>Mature_386_residues
SVIKMTDLDLAGKRVLIRADLNVPVKDGKVTSDARIRASLPTIELALKQGARVMVTSHLGRPTEGEYNAEFSLLPVVNYL
KDHLKASVRLAKDYLDGVDVAQGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAPIA
CAGPLLSGELEALGKALSNPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHNVGKSLYEADLIPE
AKKLLGTCDIPVPTDVRVATEFSETASATMKSVSEIKDDEQVLDLGDASAEQLAAILKNAKTILWNGPVGVFEFPNFRKG
TEIVANAIANSDAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKKLPAVVMLEERAKQ

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=36.8292682926829, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=36.5853658536585, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI1789294, Length=387, Percent_Identity=89.405684754522, Blast_Score=705, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17508823, Length=411, Percent_Identity=40.632603406326, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6319857, Length=417, Percent_Identity=41.0071942446043, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=39.5631067961165, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=38.0722891566265, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40926; Mature: 40795

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00111 PGLYCERATE_KINASE ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKMTDLDLAGKRVLIRADLNVPVKDGKVTSDARIRASLPTIELALKQGARVMVTSHL
CCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPTEGEYNAEFSLLPVVNYLKDHLKASVRLAKDYLDGVDVAQGELVVLENVRFNKGEKK
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
DDETLSKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAPIACAGPLLSGELEALGKALSN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCC
PARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHNVGKSLYEADLIP
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCHHHHHHHHHHCCH
EAKKLLGTCDIPVPTDVRVATEFSETASATMKSVSEIKDDEQVLDLGDASAEQLAAILKN
HHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFPNFRKGTEIVANAIANSDAFSIAGGGDTLAAIDLFGIADKISYIS
CCEEEECCCCCEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEHHHHHHHHHHHEEE
TGGGAFLEFVEGKKLPAVVMLEERAKQ
CCCCHHHHHHCCCCCCEEEEEHHHCCC
>Mature Secondary Structure 
SVIKMTDLDLAGKRVLIRADLNVPVKDGKVTSDARIRASLPTIELALKQGARVMVTSHL
CEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPTEGEYNAEFSLLPVVNYLKDHLKASVRLAKDYLDGVDVAQGELVVLENVRFNKGEKK
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
DDETLSKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAPIACAGPLLSGELEALGKALSN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCC
PARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAEGHNVGKSLYEADLIP
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCHHHHHHHHHHCCH
EAKKLLGTCDIPVPTDVRVATEFSETASATMKSVSEIKDDEQVLDLGDASAEQLAAILKN
HHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFPNFRKGTEIVANAIANSDAFSIAGGGDTLAAIDLFGIADKISYIS
CCEEEECCCCCEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEHHHHHHHHHHHEEE
TGGGAFLEFVEGKKLPAVVMLEERAKQ
CCCCHHHHHHCCCCCCEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA