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The map label for this gene is hycE [H]

Identifier: 238921132

GI number: 238921132

Start: 3152165

End: 3153865

Strand: Direct

Name: hycE [H]

Synonym: NT01EI_3273

Alternate gene names: 238921132

Gene position: 3152165-3153865 (Clockwise)

Preceding gene: 238921131

Following gene: 238921133

Centisome position: 82.68

GC content: 63.55

Gene sequence:

>1701_bases
ATGACACAACACACCGGACAACACTACATTGCGGCGCTGCGCCAACAGTTCCCCCACGCCATTATCGAGGAGAGCTGGCA
GACCGATACCCAGACCACCATCACCGTCAAGACCGATATGCTGCCGGAGGTGGTTGAATACCTGTATTACCAGCAGGGCG
GCTGGCTTTCCGTCCTGTTCGGCAACGATGAGCGCCCACTGTGCGGCAGCTACGCGGTCTACTACGTTCTGTCGATGGAG
CGCGGCACCAAGTGCTGGATAACCGTCAAAGCGCTGGTCGACGCCACGACCCGCGAATACCCGTCGGTCACGCCGCGGGT
ACCCGCCGCCGTGTGGGGCGAGCGCGAAGTCCGCGACATGTATGGGCTTATCGCCGTCGGCCTGCCGGACGAGCGCCGCC
TGGTGCTGCCGGACGATTGGCCGGACGATCTCTACCCGCTGCGCAAGGATGCGATGGACTATCGTCAGCGCCCGGCGCCG
ACCACCGATCGCGAGACCTACCCCTTCGTCAACGACAGCAGCAGCGGTAAAATCGTTCCCATCGGCCCGCTGCACATCAC
CTCTGACGAGCCGGGCCACTTCCGCCTGTTCGTGGACGGCGAGGACATCGTCGACGCCGACTATCGCCTGTTCTATGTCC
ACCGCGGCATGGAAAAGCTGGCGGAGACCCGCATGGGCTACAACGAGGTCACCTTCCTCTCCGACCGCGTCTGCGGTATC
TGCGGCTTTGCCCACAGCACCGCCTACACCAGCTCGGTCGAAAACGCGCTGGGCATCCAGGTGCCACAGCGCGCCCAAGC
CATCCGCTCCATCCTGCTGGAGGTGGAGCGCCTGCACAGCCACCTGCTCAATCTGGGACTCTCCTGCCACTTCGTCGGCT
TCGACTCGGGCTTCATGCAGTTCTTCCGGGTACGTGAAAAATCGATGAAGATGGCGGAGATCCTCACCGGCTCGCGCAAA
ACCTACGGCCTGAACCTGATCGGCGGCATCCGTCGCGACATGCTCAAAGAGGATATGATCGCCACCCGCCGTCTGGCCGC
AGAGATGCGCGCCGAGGTCAAAACCCTGGTCGACATCCTGTTCAGCACCCCCAATATCGAACAGCGCACCGTCGGTATCG
GCCGCCTCGACCCGCAGATCGCCCGCGACTTCAGCAACGTCGGCCCGATGGTGCGCGCCAGCGGCCACGCGCGCGATACC
CGCGCCGACCATCCGTTCGCCGGTTACGATCTGCTGCCGATGCAGGTTCACAGCGAGCAGGGCTGCGACGTCATCTCACG
CGTCAAGGTGCGCATTAACGAGGTGTTTACCGCCCTCAACATGATCGAGTTCGGCCTGGACAACCTGCCGGGCGGTCCGC
TGGCGGTCGAGGGCTTTACCTATGTTCCGCAGCGCTTTGCCCTGGGCTTTACCGAGGCGCCGCGCGGCGACGACATCCAC
TGGTCGATGACCGGCGACAACCAGAAGCTGTACCGCTGGCGCTGCCGGGCAGCGACCTATGCCAACTGGCCGACCCTGCG
CTACATGCTGCGCGGCAACACCGTATCCGATGCGCCGCTGATCATCGGCAGTCTCGACCCCTGTTACTCCTGTACCGACC
GCGTCACCCTGGTGGACGTCAACCGACGCAAATCGGTCACCGTGCCCTATAAGGAGATCGAGCGCTATGGACTGGAGCGC
AAGGACTCACCGCTGAAGTGA

Upstream 100 bases:

>100_bases
GTTCTTCTCTTGGCTGATCGCGTGAGGCGAACCATGACATCCATCGCCACGACGACCCGTTTTTCCTTTTTTGGCACAAC
CCATGAGGCCGCAAAGTAAA

Downstream 100 bases:

>100_bases
TCGCCGGAGACGAGAGACTATGCTGAAACTGTTAAAAACTGCCCTGCGTCATCGCCATGCTACCGTCGCCTATCCGGCGC
GTCCGCTGGATGTCGACGCC

Product: hydrogenase-4 component G

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MTQHTGQHYIAALRQQFPHAIIEESWQTDTQTTITVKTDMLPEVVEYLYYQQGGWLSVLFGNDERPLCGSYAVYYVLSME
RGTKCWITVKALVDATTREYPSVTPRVPAAVWGEREVRDMYGLIAVGLPDERRLVLPDDWPDDLYPLRKDAMDYRQRPAP
TTDRETYPFVNDSSSGKIVPIGPLHITSDEPGHFRLFVDGEDIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGI
CGFAHSTAYTSSVENALGIQVPQRAQAIRSILLEVERLHSHLLNLGLSCHFVGFDSGFMQFFRVREKSMKMAEILTGSRK
TYGLNLIGGIRRDMLKEDMIATRRLAAEMRAEVKTLVDILFSTPNIEQRTVGIGRLDPQIARDFSNVGPMVRASGHARDT
RADHPFAGYDLLPMQVHSEQGCDVISRVKVRINEVFTALNMIEFGLDNLPGGPLAVEGFTYVPQRFALGFTEAPRGDDIH
WSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVNRRKSVTVPYKEIERYGLER
KDSPLK

Sequences:

>Translated_566_residues
MTQHTGQHYIAALRQQFPHAIIEESWQTDTQTTITVKTDMLPEVVEYLYYQQGGWLSVLFGNDERPLCGSYAVYYVLSME
RGTKCWITVKALVDATTREYPSVTPRVPAAVWGEREVRDMYGLIAVGLPDERRLVLPDDWPDDLYPLRKDAMDYRQRPAP
TTDRETYPFVNDSSSGKIVPIGPLHITSDEPGHFRLFVDGEDIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGI
CGFAHSTAYTSSVENALGIQVPQRAQAIRSILLEVERLHSHLLNLGLSCHFVGFDSGFMQFFRVREKSMKMAEILTGSRK
TYGLNLIGGIRRDMLKEDMIATRRLAAEMRAEVKTLVDILFSTPNIEQRTVGIGRLDPQIARDFSNVGPMVRASGHARDT
RADHPFAGYDLLPMQVHSEQGCDVISRVKVRINEVFTALNMIEFGLDNLPGGPLAVEGFTYVPQRFALGFTEAPRGDDIH
WSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVNRRKSVTVPYKEIERYGLER
KDSPLK
>Mature_565_residues
TQHTGQHYIAALRQQFPHAIIEESWQTDTQTTITVKTDMLPEVVEYLYYQQGGWLSVLFGNDERPLCGSYAVYYVLSMER
GTKCWITVKALVDATTREYPSVTPRVPAAVWGEREVRDMYGLIAVGLPDERRLVLPDDWPDDLYPLRKDAMDYRQRPAPT
TDRETYPFVNDSSSGKIVPIGPLHITSDEPGHFRLFVDGEDIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGIC
GFAHSTAYTSSVENALGIQVPQRAQAIRSILLEVERLHSHLLNLGLSCHFVGFDSGFMQFFRVREKSMKMAEILTGSRKT
YGLNLIGGIRRDMLKEDMIATRRLAAEMRAEVKTLVDILFSTPNIEQRTVGIGRLDPQIARDFSNVGPMVRASGHARDTR
ADHPFAGYDLLPMQVHSEQGCDVISRVKVRINEVFTALNMIEFGLDNLPGGPLAVEGFTYVPQRFALGFTEAPRGDDIHW
SMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVNRRKSVTVPYKEIERYGLERK
DSPLK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=419, Percent_Identity=24.1050119331742, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI260898743, Length=411, Percent_Identity=24.5742092457421, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1789076, Length=566, Percent_Identity=83.3922261484099, Blast_Score=1014, Evalue=0.0,
Organism=Escherichia coli, GI1788832, Length=566, Percent_Identity=73.1448763250883, Blast_Score=870, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=604, Percent_Identity=25.3311258278146, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17568379, Length=403, Percent_Identity=25.5583126550869, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17555284, Length=403, Percent_Identity=25.3101736972705, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI221459469, Length=402, Percent_Identity=25.3731343283582, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24638644, Length=392, Percent_Identity=25.765306122449, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 64280; Mature: 64149

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHTGQHYIAALRQQFPHAIIEESWQTDTQTTITVKTDMLPEVVEYLYYQQGGWLSVLF
CCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEEE
GNDERPLCGSYAVYYVLSMERGTKCWITVKALVDATTREYPSVTPRVPAAVWGEREVRDM
CCCCCCCCCCEEEEEEEEECCCCEEEEEEEHHHHHHHHCCCCCCCCCCHHHCCCHHHHHH
YGLIAVGLPDERRLVLPDDWPDDLYPLRKDAMDYRQRPAPTTDRETYPFVNDSSSGKIVP
HCEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
IGPLHITSDEPGHFRLFVDGEDIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGI
ECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHHHHH
CGFAHSTAYTSSVENALGIQVPQRAQAIRSILLEVERLHSHLLNLGLSCHFVGFDSGFMQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
FFRVREKSMKMAEILTGSRKTYGLNLIGGIRRDMLKEDMIATRRLAAEMRAEVKTLVDIL
HHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTPNIEQRTVGIGRLDPQIARDFSNVGPMVRASGHARDTRADHPFAGYDLLPMQVHSEQ
HCCCCCCHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCC
GCDVISRVKVRINEVFTALNMIEFGLDNLPGGPLAVEGFTYVPQRFALGFTEAPRGDDIH
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHCCCCCCCCCCCEE
WSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDV
EEEECCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHCCCCCEEEEEC
NRRKSVTVPYKEIERYGLERKDSPLK
CCCCEEECCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TQHTGQHYIAALRQQFPHAIIEESWQTDTQTTITVKTDMLPEVVEYLYYQQGGWLSVLF
CCCCHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEEE
GNDERPLCGSYAVYYVLSMERGTKCWITVKALVDATTREYPSVTPRVPAAVWGEREVRDM
CCCCCCCCCCEEEEEEEEECCCCEEEEEEEHHHHHHHHCCCCCCCCCCHHHCCCHHHHHH
YGLIAVGLPDERRLVLPDDWPDDLYPLRKDAMDYRQRPAPTTDRETYPFVNDSSSGKIVP
HCEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
IGPLHITSDEPGHFRLFVDGEDIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVCGI
ECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHHHHH
CGFAHSTAYTSSVENALGIQVPQRAQAIRSILLEVERLHSHLLNLGLSCHFVGFDSGFMQ
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
FFRVREKSMKMAEILTGSRKTYGLNLIGGIRRDMLKEDMIATRRLAAEMRAEVKTLVDIL
HHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTPNIEQRTVGIGRLDPQIARDFSNVGPMVRASGHARDTRADHPFAGYDLLPMQVHSEQ
HCCCCCCHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCC
GCDVISRVKVRINEVFTALNMIEFGLDNLPGGPLAVEGFTYVPQRFALGFTEAPRGDDIH
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHCCCCCCCCCCCEE
WSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDV
EEEECCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHCCCCCEEEEEC
NRRKSVTVPYKEIERYGLERKDSPLK
CCCCEEECCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]