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The map label for this gene is mltD [H]

Identifier: 238921066

GI number: 238921066

Start: 3073923

End: 3074987

Strand: Direct

Name: mltD [H]

Synonym: NT01EI_3197

Alternate gene names: 238921066

Gene position: 3073923-3074987 (Clockwise)

Preceding gene: 238921065

Following gene: 238921067

Centisome position: 80.63

GC content: 55.68

Gene sequence:

>1065_bases
ATGTACTGGATTGTCGAGCAGATTAAGAAACGTAATATGCCGATGGAACTGGTACTGCTACCCATAGTGGAGAGCGCTTT
TAATCCGCATGCAACGTCATCGGCCAACGCCGCAGGGCTATGGCAGATCGTGCCTCAAACGGGTCGTAACTACGGACTGA
AACAGAACCAGTGGTATGATGGCCGCCGGGATATCGCCGCCTCGACAACGGCTGCGCTGGATATGATGCAGCGTATGAAC
AAGATGTTCGACGGCGACTGGCTGCTAACCCTTGCTGCATTCAACAGTGGCGAAGGGCGCGTTATGCAGGCGATCAAGGC
CAACAAGCGCAAAGGGCGCCCGACCGACTATTGGTCTCTCTCCCTGCCGCGTGAGACGGCGCTGTATGTACCAAAGGTGC
TGGCGCTGAGCGATATCATCAAGCATAACCAGCGCTATGGTATCAAACTGCCTAAGTCGAGTCAGGAACGGGCGCTGGCA
CGCGTTGACGTCAGTCAGCAGATGCAGCTTAGCCAGGCCGCCGAGATGGCCGGGATGTCGCTGACCAAGCTGAAGTCGTT
CAACCCTGGATACAAGCGTAACGTTACCGCACCGAGTGGGCACGGCCCGCGCTATATAATGGTGCCTCAGGCCCACGTCG
ATCGACTGAAGGACTCGCTGGCAGAGCGAGAAATTGCCGCGATCCAGCCGGATACTGAGCTGGCGAACCATCGCAGCACG
TCGACTTATCGAGTACGCAACGGCGATACACTGTCTGGTATTGCCCGTCGCCTAAACATGACGCAGAAAACGCTGCTGAG
CATGAATAATATGGGCAGCCGCAGCATCCTGAAAGCCGGACAGACTCTAAAGGTCGCGACAGGTGCGAAAGGAACGGCAC
TGGCGGACAATACCATCACCTATAAGGTGCGGAAAGGGGACTCACTCTTCAGCATTGCACGCCGCCACGGCGTCGATATT
GATGATGTAATGCGTTGGAACAGCAGGGTTAACGATAACGGCCAGATCCAGCCGGGGGACTTGCTGACGTTGTATGTGAA
AGATAAGCAAGACGGCAATAGTTGA

Upstream 100 bases:

>100_bases
GATAAAGGTTCCGAACAATACCCGGATCCGCGAGCAACGAAACAAGTATTTACGCAATAAGAGCTATCTCCACGATGTAA
CATTACGGGCAGAGCCGTAC

Downstream 100 bases:

>100_bases
CGTTTTCCTGCACAAAAAAAGAGCCTGTACATAAATTTGTGTAATTGCCTGATTTTGATATGTTCAATCCAACATCAAAA
GCAGGTTACTTTATGAACGA

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MYWIVEQIKKRNMPMELVLLPIVESAFNPHATSSANAAGLWQIVPQTGRNYGLKQNQWYDGRRDIAASTTAALDMMQRMN
KMFDGDWLLTLAAFNSGEGRVMQAIKANKRKGRPTDYWSLSLPRETALYVPKVLALSDIIKHNQRYGIKLPKSSQERALA
RVDVSQQMQLSQAAEMAGMSLTKLKSFNPGYKRNVTAPSGHGPRYIMVPQAHVDRLKDSLAEREIAAIQPDTELANHRST
STYRVRNGDTLSGIARRLNMTQKTLLSMNNMGSRSILKAGQTLKVATGAKGTALADNTITYKVRKGDSLFSIARRHGVDI
DDVMRWNSRVNDNGQIQPGDLLTLYVKDKQDGNS

Sequences:

>Translated_354_residues
MYWIVEQIKKRNMPMELVLLPIVESAFNPHATSSANAAGLWQIVPQTGRNYGLKQNQWYDGRRDIAASTTAALDMMQRMN
KMFDGDWLLTLAAFNSGEGRVMQAIKANKRKGRPTDYWSLSLPRETALYVPKVLALSDIIKHNQRYGIKLPKSSQERALA
RVDVSQQMQLSQAAEMAGMSLTKLKSFNPGYKRNVTAPSGHGPRYIMVPQAHVDRLKDSLAEREIAAIQPDTELANHRST
STYRVRNGDTLSGIARRLNMTQKTLLSMNNMGSRSILKAGQTLKVATGAKGTALADNTITYKVRKGDSLFSIARRHGVDI
DDVMRWNSRVNDNGQIQPGDLLTLYVKDKQDGNS
>Mature_354_residues
MYWIVEQIKKRNMPMELVLLPIVESAFNPHATSSANAAGLWQIVPQTGRNYGLKQNQWYDGRRDIAASTTAALDMMQRMN
KMFDGDWLLTLAAFNSGEGRVMQAIKANKRKGRPTDYWSLSLPRETALYVPKVLALSDIIKHNQRYGIKLPKSSQERALA
RVDVSQQMQLSQAAEMAGMSLTKLKSFNPGYKRNVTAPSGHGPRYIMVPQAHVDRLKDSLAEREIAAIQPDTELANHRST
STYRVRNGDTLSGIARRLNMTQKTLLSMNNMGSRSILKAGQTLKVATGAKGTALADNTITYKVRKGDSLFSIARRHGVDI
DDVMRWNSRVNDNGQIQPGDLLTLYVKDKQDGNS

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=353, Percent_Identity=60.9065155807365, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 39537; Mature: 39537

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYWIVEQIKKRNMPMELVLLPIVESAFNPHATSSANAAGLWQIVPQTGRNYGLKQNQWYD
CCHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
GRRDIAASTTAALDMMQRMNKMFDGDWLLTLAAFNSGEGRVMQAIKANKRKGRPTDYWSL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEE
SLPRETALYVPKVLALSDIIKHNQRYGIKLPKSSQERALARVDVSQQMQLSQAAEMAGMS
ECCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHCHHHHHHHHHHHHHHCCH
LTKLKSFNPGYKRNVTAPSGHGPRYIMVPQAHVDRLKDSLAEREIAAIQPDTELANHRST
HHHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
STYRVRNGDTLSGIARRLNMTQKTLLSMNNMGSRSILKAGQTLKVATGAKGTALADNTIT
CEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCEEECCEEE
YKVRKGDSLFSIARRHGVDIDDVMRWNSRVNDNGQIQPGDLLTLYVKDKQDGNS
EEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MYWIVEQIKKRNMPMELVLLPIVESAFNPHATSSANAAGLWQIVPQTGRNYGLKQNQWYD
CCHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
GRRDIAASTTAALDMMQRMNKMFDGDWLLTLAAFNSGEGRVMQAIKANKRKGRPTDYWSL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEE
SLPRETALYVPKVLALSDIIKHNQRYGIKLPKSSQERALARVDVSQQMQLSQAAEMAGMS
ECCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHCHHHHHHHHHHHHHHCCH
LTKLKSFNPGYKRNVTAPSGHGPRYIMVPQAHVDRLKDSLAEREIAAIQPDTELANHRST
HHHHHCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
STYRVRNGDTLSGIARRLNMTQKTLLSMNNMGSRSILKAGQTLKVATGAKGTALADNTIT
CEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCEEECCEEE
YKVRKGDSLFSIARRHGVDIDDVMRWNSRVNDNGQIQPGDLLTLYVKDKQDGNS
EEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]