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The map label for this gene is sigF [H]

Identifier: 238917528

GI number: 238917528

Start: 1661505

End: 1662254

Strand: Reverse

Name: sigF [H]

Synonym: EUBELI_01607

Alternate gene names: 238917528

Gene position: 1662254-1661505 (Counterclockwise)

Preceding gene: 238917529

Following gene: 238917527

Centisome position: 77.52

GC content: 42.13

Gene sequence:

>750_bases
TTGATAGGATGTGATGCTATGGATCAGACTAAGGATTGTACCAGGGAATTGATCGCTAAAGCACACAATAATGACAAAGA
GGCCAGAGAGAAGCTGGTAACAGATAATCTAGGGCTTATATGGAACATAATTAAAAGATTTAAGGGAAGAGGAACAGAAC
AGGAGGATCTGTTTCAGATAGGATGTATAGGACTTATAAAGGCAATTGATAATTTCAATCTGGATATGGATGTTGCATTT
TCAACATATGCAGTTCCAATGATAACAGGTGAGATAAGAAGATTTCTGCGGGATGATGGAATGGTAAAGGTCAGCCGTTC
TATCAAAGAAAATGCAGTAAAAAGCATGAAAGCGGCGGGACAGCTCCGTGACACATTAGGCTGCGAACCGACAGTAGAAC
AGATAGCAGCTGCCTGTGAGTTGTCAGTTGAAGACATAACACTGTCGATGTCTGCATCAATGGAGGTTGAGTCAATATAT
AAGACAATCTATCAGGGGGATGGAAGTGAGGTCATGCTGATGGATAAGTTAGAAGATAAGGCAGACATTGGGGAAACTGC
GTCAAGTAAAGTGGCACTTGTACAACTTATGGAAACACTTTCACAGCGTGAAAAAGAGCTTATCAGGATGCGTTATTATG
ATGATTACACACAGATGCAGGTTGCTAAGGAGTTCGGAATATCGCAGGTGCAGGTGTCGCGGATGGAGAAGAAGATTTTA
AGTGGCATGCGCAAGCGGCTGGTTGTATGA

Upstream 100 bases:

>100_bases
GGGATTTTCGTTCATGGAGGCATTCATGGATGATTTAAGAGTTGCGTCAGCACCAGGCAAAGGAACAACAGTGCTTATGA
GAAAGAAAATAGGGGAGTAA

Downstream 100 bases:

>100_bases
ATAAATTTTTGGGTATGTACAATACTAATACTAGCAACGGAGGGTTTGTTATGTCAGAGAAGATTTATTTGAAGATTGAC
CAGCAAAGGTATGTTAAGAA

Product: RNA polymerase sporulation-specific sigma factor

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIGCDAMDQTKDCTRELIAKAHNNDKEAREKLVTDNLGLIWNIIKRFKGRGTEQEDLFQIGCIGLIKAIDNFNLDMDVAF
STYAVPMITGEIRRFLRDDGMVKVSRSIKENAVKSMKAAGQLRDTLGCEPTVEQIAAACELSVEDITLSMSASMEVESIY
KTIYQGDGSEVMLMDKLEDKADIGETASSKVALVQLMETLSQREKELIRMRYYDDYTQMQVAKEFGISQVQVSRMEKKIL
SGMRKRLVV

Sequences:

>Translated_249_residues
MIGCDAMDQTKDCTRELIAKAHNNDKEAREKLVTDNLGLIWNIIKRFKGRGTEQEDLFQIGCIGLIKAIDNFNLDMDVAF
STYAVPMITGEIRRFLRDDGMVKVSRSIKENAVKSMKAAGQLRDTLGCEPTVEQIAAACELSVEDITLSMSASMEVESIY
KTIYQGDGSEVMLMDKLEDKADIGETASSKVALVQLMETLSQREKELIRMRYYDDYTQMQVAKEFGISQVQVSRMEKKIL
SGMRKRLVV
>Mature_249_residues
MIGCDAMDQTKDCTRELIAKAHNNDKEAREKLVTDNLGLIWNIIKRFKGRGTEQEDLFQIGCIGLIKAIDNFNLDMDVAF
STYAVPMITGEIRRFLRDDGMVKVSRSIKENAVKSMKAAGQLRDTLGCEPTVEQIAAACELSVEDITLSMSASMEVESIY
KTIYQGDGSEVMLMDKLEDKADIGETASSKVALVQLMETLSQREKELIRMRYYDDYTQMQVAKEFGISQVQVSRMEKKIL
SGMRKRLVV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with spoIIAB inhibits sig

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=251, Percent_Identity=29.8804780876494, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1789098, Length=236, Percent_Identity=25, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789871, Length=259, Percent_Identity=27.4131274131274, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28077; Mature: 28077

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGCDAMDQTKDCTRELIAKAHNNDKEAREKLVTDNLGLIWNIIKRFKGRGTEQEDLFQI
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
GCIGLIKAIDNFNLDMDVAFSTYAVPMITGEIRRFLRDDGMVKVSRSIKENAVKSMKAAG
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QLRDTLGCEPTVEQIAAACELSVEDITLSMSASMEVESIYKTIYQGDGSEVMLMDKLEDK
HHHHHCCCCHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHCCCCCCEEEEHHHCCH
ADIGETASSKVALVQLMETLSQREKELIRMRYYDDYTQMQVAKEFGISQVQVSRMEKKIL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SGMRKRLVV
HHHHHHCCC
>Mature Secondary Structure
MIGCDAMDQTKDCTRELIAKAHNNDKEAREKLVTDNLGLIWNIIKRFKGRGTEQEDLFQI
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
GCIGLIKAIDNFNLDMDVAFSTYAVPMITGEIRRFLRDDGMVKVSRSIKENAVKSMKAAG
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QLRDTLGCEPTVEQIAAACELSVEDITLSMSASMEVESIYKTIYQGDGSEVMLMDKLEDK
HHHHHCCCCHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHCCCCCCEEEEHHHCCH
ADIGETASSKVALVQLMETLSQREKELIRMRYYDDYTQMQVAKEFGISQVQVSRMEKKIL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SGMRKRLVV
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3116160; 6088674; 8969508; 9384377; 8358793 [H]