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The map label for this gene is brnQ [H]

Identifier: 238893325

GI number: 238893325

Start: 1167195

End: 1168514

Strand: Direct

Name: brnQ [H]

Synonym: KP1_1203

Alternate gene names: 238893325

Gene position: 1167195-1168514 (Clockwise)

Preceding gene: 238893324

Following gene: 238893326

Centisome position: 22.24

GC content: 60.76

Gene sequence:

>1320_bases
ATGACCCATCAGTTAAAATCTCGCGACATCATAGCGTTGGGCTTTATGACCTTCGCGTTGTTCGTTGGCGCAGGCAACAT
TATTTTCCCTCCGATGGTTGGCTTACAGGCGGGCGAGCACGTCTGGACGGCGGCGATCGGCTTCCTGATCACTGCGGTGG
GTCTGCCGGTGCTGACGGTGGTGGCGCTGGCGAAAGTCGGCGGCGGCGTTGAAAGCCTGAGCACCCCGATTGGTAAAGTG
GCCGGTATCCTGCTGGCGGTGGTGTGCTATCTCGCGGTAGGACCGCTGTTCGCGACGCCGCGTACCGCGACCGTCTCCTT
TGAAGTCGGTATTGCCCCGCTGACCGGCGACGGCCCGCTGCCGCTGCTGATCTACAGCGTTATTTACTTCGCGCTGGTGA
TTCTGGTCTCCCTCTATCCGGGCAAACTGCTGGACACCGTGGGCAATTTCCTTGCGCCGCTGAAAATTATCGCCCTGATT
GTGCTGGCGGTCGCCGCCATCATCTGGCCAGCGGGCCCGATCAGCGACGCGCTGGAAGCGTATCGTACGGCGCCATTCTC
CAATGGCTTCGTCAACGGCTACCTGACCATGGATACCCTCGGCGCGATGGTATTTGGCATCGTGATTGTTAATGCCGCTC
GCTCACGCGGCGTCAGCGAAGCGCGTCTGTTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGCGTCGGCCTGACC
CTGCTGTATCTGGCGCTGTTCCGTCTGGGCTCTGACAGCGCTACTCTCGTGGATCAGTCCGCCAACGGCGCGGCGATCCT
CCATGCCTACGTGCAGCACACCTTCGGCGGGGCGGGCAGTTTCCTGCTGGCGGCGCTGATCTTCATCGCTTGCCTGGTGA
CGGCGGTGGGCCTGACCTGCGCCTGCGCTGAGTTCTTTGCCCAGTATCTGCCGTTCTCCTATCGCACGCTGGTCTTTGTT
CTCGGCCTGTTCTCCATGGCGGTCTCCAATTTGGGCCTTAGCCATCTGATCCAGGTATCGATTCCGGTGCTGACCGCTAT
CTATCCGCCGTGTATCGCACTGGTTGTATTAAGCTTTACCCGCAGTTGGTGGCATAATTCGTCCCGCGTTATCGCGCCGG
CCATGTTTATCAGCCTGATGTTTGGTATCATTGACGGGATTAAGTCATCGGCATTTGCCGCGATTCTGCCGGACTGGACC
GCGCGTCTGCCGCTGGCGGAGCAGGGCCTGGCCTGGCTGATGCCCACCGCCGTGATGACCATTCTCGCGGTGATCTGGGA
TCGCGCAGCCGGACGTCAGGTGACTTCCAGCGCGCATTAA

Upstream 100 bases:

>100_bases
GCTGAGTTTACCGGCAATGGCCGCTGTATCGTCCCTGAGAGGGGAGCGCGGTATTTTTCCATTTTTTTACAACACCACAT
CCACAGGCAGTAATGAATTT

Downstream 100 bases:

>100_bases
CCGGCGGTGAGGACAAGCGTTTAACCACGGGACTTCGGGTTCCGTGGTTTTTTATTTTTTGTGTTAAACGGCATTGAAAA
ACCACGATGGAAAGTAGTAA

Product: branched chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTHQLKSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAIGFLITAVGLPVLTVVALAKVGGGVESLSTPIGKV
AGILLAVVCYLAVGPLFATPRTATVSFEVGIAPLTGDGPLPLLIYSVIYFALVILVSLYPGKLLDTVGNFLAPLKIIALI
VLAVAAIIWPAGPISDALEAYRTAPFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLT
LLYLALFRLGSDSATLVDQSANGAAILHAYVQHTFGGAGSFLLAALIFIACLVTAVGLTCACAEFFAQYLPFSYRTLVFV
LGLFSMAVSNLGLSHLIQVSIPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPAMFISLMFGIIDGIKSSAFAAILPDWT
ARLPLAEQGLAWLMPTAVMTILAVIWDRAAGRQVTSSAH

Sequences:

>Translated_439_residues
MTHQLKSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAIGFLITAVGLPVLTVVALAKVGGGVESLSTPIGKV
AGILLAVVCYLAVGPLFATPRTATVSFEVGIAPLTGDGPLPLLIYSVIYFALVILVSLYPGKLLDTVGNFLAPLKIIALI
VLAVAAIIWPAGPISDALEAYRTAPFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLT
LLYLALFRLGSDSATLVDQSANGAAILHAYVQHTFGGAGSFLLAALIFIACLVTAVGLTCACAEFFAQYLPFSYRTLVFV
LGLFSMAVSNLGLSHLIQVSIPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPAMFISLMFGIIDGIKSSAFAAILPDWT
ARLPLAEQGLAWLMPTAVMTILAVIWDRAAGRQVTSSAH
>Mature_438_residues
THQLKSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAIGFLITAVGLPVLTVVALAKVGGGVESLSTPIGKVA
GILLAVVCYLAVGPLFATPRTATVSFEVGIAPLTGDGPLPLLIYSVIYFALVILVSLYPGKLLDTVGNFLAPLKIIALIV
LAVAAIIWPAGPISDALEAYRTAPFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLTL
LYLALFRLGSDSATLVDQSANGAAILHAYVQHTFGGAGSFLLAALIFIACLVTAVGLTCACAEFFAQYLPFSYRTLVFVL
GLFSMAVSNLGLSHLIQVSIPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPAMFISLMFGIIDGIKSSAFAAILPDWTA
RLPLAEQGLAWLMPTAVMTILAVIWDRAAGRQVTSSAH

Specific function: Component of the LIV-II transport system for branched- chain amino acids. This LIV-II transport system may be H(+)- coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=439, Percent_Identity=89.749430523918, Blast_Score=793, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46219; Mature: 46087

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHQLKSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAIGFLITAVGLPVLTV
CCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VALAKVGGGVESLSTPIGKVAGILLAVVCYLAVGPLFATPRTATVSFEVGIAPLTGDGPL
HHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECEEEECCCCCH
PLLIYSVIYFALVILVSLYPGKLLDTVGNFLAPLKIIALIVLAVAAIIWPAGPISDALEA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
YRTAPFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLT
HHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLYLALFRLGSDSATLVDQSANGAAILHAYVQHTFGGAGSFLLAALIFIACLVTAVGLTC
HHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ACAEFFAQYLPFSYRTLVFVLGLFSMAVSNLGLSHLIQVSIPVLTAIYPPCIALVVLSFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSWWHNSSRVIAPAMFISLMFGIIDGIKSSAFAAILPDWTARLPLAEQGLAWLMPTAVMT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHCCHHHHHHHHHHH
ILAVIWDRAAGRQVTSSAH
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
THQLKSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAIGFLITAVGLPVLTV
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VALAKVGGGVESLSTPIGKVAGILLAVVCYLAVGPLFATPRTATVSFEVGIAPLTGDGPL
HHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECEEEECCCCCH
PLLIYSVIYFALVILVSLYPGKLLDTVGNFLAPLKIIALIVLAVAAIIWPAGPISDALEA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
YRTAPFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVSEARLLTRYTVWAGLMAGVGLT
HHCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLYLALFRLGSDSATLVDQSANGAAILHAYVQHTFGGAGSFLLAALIFIACLVTAVGLTC
HHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ACAEFFAQYLPFSYRTLVFVLGLFSMAVSNLGLSHLIQVSIPVLTAIYPPCIALVVLSFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSWWHNSSRVIAPAMFISLMFGIIDGIKSSAFAAILPDWTARLPLAEQGLAWLMPTAVMT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHCCHHHHHHHHHHH
ILAVIWDRAAGRQVTSSAH
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]