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The map label for this gene is phoB [H]

Identifier: 238893323

GI number: 238893323

Start: 1164775

End: 1165464

Strand: Direct

Name: phoB [H]

Synonym: KP1_1200

Alternate gene names: 238893323

Gene position: 1164775-1165464 (Clockwise)

Preceding gene: 238893320

Following gene: 238893324

Centisome position: 22.19

GC content: 56.09

Gene sequence:

>690_bases
ATGGCGAGAAGAATTCTGGTCGTTGAAGATGAAGCTCCAATCCGCGAAATGGTCTGCTTTGTTCTGGAGCAAAATGGCTT
TCAGCCTGTCGAAGCGGAAGATTATGACAGCGCGGTGAATCAACTCAATGAACCCTGGCCTGATTTGATTCTCCTGGACT
GGATGCTGCCGGGCGGTTCGGGGCTGCAGTTTATTAAACTGCTCAAGCGCGAGGCGATGACGCGGGATATTCCGGTAGTG
ATGCTGACCGCGCGTGGAGAAGAAGAGGATCGCGTTCGCGGCCTGGAGACCGGCGCGGATGACTACATCACCAAGCCTTT
CTCTCCGAAAGAGCTGGTGGCGCGAATCAAAGCGGTGATGCGCCGTATTTCACCGATGGCGGTGGAAGAGGTGATCGAAA
TGCAGGGGCTGAGCCTCGACCCTTCATCGCACCGGGTAATGACCGGCGACAGTCCGCTGGATATGGGACCGACGGAATTT
AAATTATTGCACTTCTTTATGACCCATCCGGAGCGGGTGTACAGCCGCGAACAGCTGCTGAACCACGTCTGGGGGACCAA
CGTTTATGTGGAAGACCGCACGGTGGACGTCCATATTCGCCGCCTGCGCAAAGCGCTGGAACACAGTGGCCATGATCGTA
TGGTGCAAACGGTTCGCGGCACGGGGTATCGTTTCTCCGCCCGTTTCTGA

Upstream 100 bases:

>100_bases
CCGATCATCAGACGGTCATTTTTTTCATAAATCTGTCACAAAAGTGACGCATAATGACGCCGCACTTCTATACTGATGGC
TAAAAAACACAGGGTAAATA

Downstream 100 bases:

>100_bases
ATTTTGACAGGAGTGTGACCCGTGCTGGAACGGCTGTCATGGAAAAGGCTGGCGCTGGAGCTCTTTTTAGCGTGTATTCC
GGCCCTGATTCTGGGGGCGT

Product: transcriptional regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGLQFIKLLKREAMTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPSSHRVMTGDSPLDMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSARF

Sequences:

>Translated_229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGLQFIKLLKREAMTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPSSHRVMTGDSPLDMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSARF
>Mature_228_residues
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGLQFIKLLKREAMTRDIPVVM
LTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPSSHRVMTGDSPLDMGPTEFK
LLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSARF

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=95.1965065502183, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=40.7079646017699, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.5545851528384, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=37.5565610859729, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=34.9775784753363, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=36, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=33.6363636363636, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=34.4827586206897, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=33.7777777777778, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=38.4279475982533, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=32.5892857142857, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787229, Length=234, Percent_Identity=32.0512820512821, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=33.7777777777778, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=27.9661016949153, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=29.5081967213115, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=35.7798165137615, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26380; Mature: 26249

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGS
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GLQFIKLLKREAMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
CHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPSSHRVMTGDSPLDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSARF
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGS
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GLQFIKLLKREAMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
CHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPSSHRVMTGDSPLDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSARF
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]