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The map label for this gene is yajF [H]

Identifier: 238893320

GI number: 238893320

Start: 1159160

End: 1160179

Strand: Direct

Name: yajF [H]

Synonym: KP1_1197

Alternate gene names: 238893320

Gene position: 1159160-1160179 (Clockwise)

Preceding gene: 238893318

Following gene: 238893323

Centisome position: 22.09

GC content: 60.88

Gene sequence:

>1020_bases
TTGAACCACAGCATGATAATTTCCACGGCTTTGTCGTTAAATCAGGCGGGCATGATAGCGAATTGTCGCTATGCTTGCAT
TGTTAATCGGGGTGAGGAGCTGAGCGTGCGTATTGGTATTGATTTAGGTGGAACAAAAACCGAAGTGATAGCCCTGAGCG
ATCAGGGAGAGCAGCTGTTTCGCCACCGGTTACCCACGCCGCGGGAGGACTACCGGCAAACCATCGAGACCATCGCCACT
CTGGTGGCGATGGCCGAGCAGGCGTCCGGACAGCAGGGTACCGTGGGGATGGGCATTCCTGGCTCAATTTCGCCTTATAC
CGGGGTGGTGAAAAACGCCAACTCGACCTGGCTCAACGGCCAGCCCTTTGATAAGGACCTCAGCCTGCGCCTTGAGCGGG
AAGTCCGGCTGGCTAACGATGCCAACTGTCTGGCGGTGTCGGAAGCGGTGGACGGCGCGGCGGCGGGGGCGCAAACGGTA
TTCGCGGTGATTATCGGCACCGGCTGCGGCGCCGGGGTGGCGCTCAACGGGCGGGCGCATATCGGCGGCAATGGTAACGC
TGGCGAATGGGGACATAACCCATTGCCGTGGATGAACGACGATGAGCTACGCTACCGTGCGGAAGTCCCTTGCTACTGCG
GCAAACAGGGGTGTATCGAAACCTTTATCTCCGGCACCGGTTTCGCCACCGACTATCAGCGGCTGAGCGGCAAAGGGTTA
ACCGGCAGCGAGATTATGCGCCTGGTGGGAGAAGGTGACGAAAAGGCTGAACTGGCGCTGAGCCGCTATGAACAGCGGCT
GGCGAAATCGCTGGCCCACGTGGTGAATATCCTCGACCCGGACGTTATCGTGCTGGGCGGCGGGATGAGCAACGTCGAAC
GGCTGTATCAGACGGTGCCGGACCTGGTGAAGCAGTGGGTCTTCGGCGGCGAGTGTGAAACGCCGATCCGCAAAGCGATG
CACGGCGACTCCAGCGGGGTACGCGGCGCGGCCTGGCTGTGGCCGCTCCAGGGGACCTGA

Upstream 100 bases:

>100_bases
CTGGCTGCCACAAGGGGTAAATGTCAGCTCGGCCAGCTGTTTTTCCATCTCCTCGGCGCGCAATTCGATATCGCGGCTGA
GACGGTAAACCATTAAATTT

Downstream 100 bases:

>100_bases
ACCGCTTCCCCGCCCCCTAACCCTCTCCCTCGGGAGAGGGTTAGGGTGAGGGGACATCGCCATAATTCAGACCTCACTCC
ACCGCAAACGCTTTGTCCAG

Product: putative NAGC-like transcriptional regulator

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNHSMIISTALSLNQAGMIANCRYACIVNRGEELSVRIGIDLGGTKTEVIALSDQGEQLFRHRLPTPREDYRQTIETIAT
LVAMAEQASGQQGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSLRLEREVRLANDANCLAVSEAVDGAAAGAQTV
FAVIIGTGCGAGVALNGRAHIGGNGNAGEWGHNPLPWMNDDELRYRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGL
TGSEIMRLVGEGDEKAELALSRYEQRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPDLVKQWVFGGECETPIRKAM
HGDSSGVRGAAWLWPLQGT

Sequences:

>Translated_339_residues
MNHSMIISTALSLNQAGMIANCRYACIVNRGEELSVRIGIDLGGTKTEVIALSDQGEQLFRHRLPTPREDYRQTIETIAT
LVAMAEQASGQQGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSLRLEREVRLANDANCLAVSEAVDGAAAGAQTV
FAVIIGTGCGAGVALNGRAHIGGNGNAGEWGHNPLPWMNDDELRYRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGL
TGSEIMRLVGEGDEKAELALSRYEQRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPDLVKQWVFGGECETPIRKAM
HGDSSGVRGAAWLWPLQGT
>Mature_339_residues
MNHSMIISTALSLNQAGMIANCRYACIVNRGEELSVRIGIDLGGTKTEVIALSDQGEQLFRHRLPTPREDYRQTIETIAT
LVAMAEQASGQQGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSLRLEREVRLANDANCLAVSEAVDGAAAGAQTV
FAVIIGTGCGAGVALNGRAHIGGNGNAGEWGHNPLPWMNDDELRYRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGL
TGSEIMRLVGEGDEKAELALSRYEQRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPDLVKQWVFGGECETPIRKAM
HGDSSGVRGAAWLWPLQGT

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=300, Percent_Identity=88.6666666666667, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1787363, Length=299, Percent_Identity=40.133779264214, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1786891, Length=272, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790522, Length=319, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787878, Length=231, Percent_Identity=26.4069264069264, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 36251; Mature: 36251

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSMIISTALSLNQAGMIANCRYACIVNRGEELSVRIGIDLGGTKTEVIALSDQGEQLF
CCCCEEEEEHHHHCCCCCEECCEEEEEECCCCEEEEEEEEECCCCCEEEEEECCCHHHHH
RHRLPTPREDYRQTIETIATLVAMAEQASGQQGTVGMGIPGSISPYTGVVKNANSTWLNG
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHEECCCCCCCCC
QPFDKDLSLRLEREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAH
CCCCCCHHEEEEEEEEECCCCCEEEEHHHHCCHHHHHHHEEEEEEECCCCCCEEECCEEE
IGGNGNAGEWGHNPLPWMNDDELRYRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGL
ECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCC
TGSEIMRLVGEGDEKAELALSRYEQRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVP
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
DLVKQWVFGGECETPIRKAMHGDSSGVRGAAWLWPLQGT
HHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MNHSMIISTALSLNQAGMIANCRYACIVNRGEELSVRIGIDLGGTKTEVIALSDQGEQLF
CCCCEEEEEHHHHCCCCCEECCEEEEEECCCCEEEEEEEEECCCCCEEEEEECCCHHHHH
RHRLPTPREDYRQTIETIATLVAMAEQASGQQGTVGMGIPGSISPYTGVVKNANSTWLNG
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHEECCCCCCCCC
QPFDKDLSLRLEREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAH
CCCCCCHHEEEEEEEEECCCCCEEEEHHHHCCHHHHHHHEEEEEEECCCCCCEEECCEEE
IGGNGNAGEWGHNPLPWMNDDELRYRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGL
ECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCC
TGSEIMRLVGEGDEKAELALSRYEQRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVP
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
DLVKQWVFGGECETPIRKAMHGDSSGVRGAAWLWPLQGT
HHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]