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The map label for this gene is pulN [H]

Identifier: 238893129

GI number: 238893129

Start: 960514

End: 961260

Strand: Reverse

Name: pulN [H]

Synonym: KP1_0978

Alternate gene names: 238893129

Gene position: 961260-960514 (Counterclockwise)

Preceding gene: 238893130

Following gene: 238893128

Centisome position: 18.31

GC content: 68.54

Gene sequence:

>747_bases
ATGAAGGCTAACTGGATCGCGGGCGGACTGCTGCTGGTCCTCTATCTGTTGTGGCTCACCGCCACCGCTCCGGCGCGCCT
GCTGGCTCCCATGCTGCCGCCCGGCGTACAGGTGGGGCAATTTAGCGGCAGCGTCTGGCGCGGCGCGGCGCAGCCGGTCT
ACTGGCATGGGGAACGGCTTGAACGACTGGCGTGGTCGCTGACCCTGGCGGGCTGGCAGGTCTCTCTCAGCGATCCCCGC
GGCGTGATGGGTCAGGCCCGGCTGTGGGGCCTTCGCGATCTGCGCCTACAGGAGGGGCGGCTCACCGCCCCCGCCAGCAT
GCTCAGCCGCTGGTTGATGTCGACTATGCCCGTCAAGGCCGCGGGGGAGGTCACCTTCAGCCTGACCGAAGGGCGGTTCA
GCGACGGACGCTGCCGGCATATCATCGCCGGAGGCGCGCAGTGGCGGCAGGCCCGACTGCACTCCCCGGTCGGCAGCCTG
GAGCTGGCCCAGGTTAATGGCACCTTCCGCTGTACGGTGGACGGCGCCGTGGCGCTGACTCTCCGTCAGGACTCTCATCA
GCTGAGCCTCAGCGGCCAGGGGACTCTGTCGCCCGATGGCCGCTATCTGTTTCGCGGCACGCTACAGCCCCGGCAGGGAA
TGCCGCCGCTGCTGGCGCTGCTGGTCACGCGCCCGACAGCGAATAACGCCCCGGGCCCGACGCCGTGGCAGCTGCAGGGA
AAATGGTTACCACAGGAGCAAAAATGA

Upstream 100 bases:

>100_bases
CCGGCGATGCGCGGCGTCAACGCGATCTCGCTGTCCGTGACCGGGCAGCCGTCGCGACCCGGCTGGGTCACGGTCAACCA
TCTGCTGCTGGAGCGCGACG

Downstream 100 bases:

>100_bases
CGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGGCAGC
TTTTACAATGTGGTGATCTA

Product: pullulanase-specific type II secretion system component N

Products: NA

Alternate protein names: Pullulanase secretion protein PULN [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKANWIAGGLLLVLYLLWLTATAPARLLAPMLPPGVQVGQFSGSVWRGAAQPVYWHGERLERLAWSLTLAGWQVSLSDPR
GVMGQARLWGLRDLRLQEGRLTAPASMLSRWLMSTMPVKAAGEVTFSLTEGRFSDGRCRHIIAGGAQWRQARLHSPVGSL
ELAQVNGTFRCTVDGAVALTLRQDSHQLSLSGQGTLSPDGRYLFRGTLQPRQGMPPLLALLVTRPTANNAPGPTPWQLQG
KWLPQEQK

Sequences:

>Translated_248_residues
MKANWIAGGLLLVLYLLWLTATAPARLLAPMLPPGVQVGQFSGSVWRGAAQPVYWHGERLERLAWSLTLAGWQVSLSDPR
GVMGQARLWGLRDLRLQEGRLTAPASMLSRWLMSTMPVKAAGEVTFSLTEGRFSDGRCRHIIAGGAQWRQARLHSPVGSL
ELAQVNGTFRCTVDGAVALTLRQDSHQLSLSGQGTLSPDGRYLFRGTLQPRQGMPPLLALLVTRPTANNAPGPTPWQLQG
KWLPQEQK
>Mature_248_residues
MKANWIAGGLLLVLYLLWLTATAPARLLAPMLPPGVQVGQFSGSVWRGAAQPVYWHGERLERLAWSLTLAGWQVSLSDPR
GVMGQARLWGLRDLRLQEGRLTAPASMLSRWLMSTMPVKAAGEVTFSLTEGRFSDGRCRHIIAGGAQWRQARLHSPVGSL
ELAQVNGTFRCTVDGAVALTLRQDSHQLSLSGQGTLSPDGRYLFRGTLQPRQGMPPLLALLVTRPTANNAPGPTPWQLQG
KWLPQEQK

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP N family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022792
- InterPro:   IPR000645 [H]

Pfam domain/function: PF01203 GSPII_N [H]

EC number: NA

Molecular weight: Translated: 27160; Mature: 27160

Theoretical pI: Translated: 11.65; Mature: 11.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKANWIAGGLLLVLYLLWLTATAPARLLAPMLPPGVQVGQFSGSVWRGAAQPVYWHGERL
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCHHCCCCCCEEECHHHH
ERLAWSLTLAGWQVSLSDPRGVMGQARLWGLRDLRLQEGRLTAPASMLSRWLMSTMPVKA
HHHHHEEEEECEEEEECCCCCCCCCHHHCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCC
AGEVTFSLTEGRFSDGRCRHIIAGGAQWRQARLHSPVGSLELAQVNGTFRCTVDGAVALT
CCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCCCEEEEEECCEEEEEECCEEEEE
LRQDSHQLSLSGQGTLSPDGRYLFRGTLQPRQGMPPLLALLVTRPTANNAPGPTPWQLQG
EECCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHEEECCCCCCCCCCCCEEECC
KWLPQEQK
CCCCCCCC
>Mature Secondary Structure
MKANWIAGGLLLVLYLLWLTATAPARLLAPMLPPGVQVGQFSGSVWRGAAQPVYWHGERL
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCHHCCCCCCEEECHHHH
ERLAWSLTLAGWQVSLSDPRGVMGQARLWGLRDLRLQEGRLTAPASMLSRWLMSTMPVKA
HHHHHEEEEECEEEEECCCCCCCCCHHHCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCC
AGEVTFSLTEGRFSDGRCRHIIAGGAQWRQARLHSPVGSLELAQVNGTFRCTVDGAVALT
CCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCCCEEEEEECCEEEEEECCEEEEE
LRQDSHQLSLSGQGTLSPDGRYLFRGTLQPRQGMPPLLALLVTRPTANNAPGPTPWQLQG
EECCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHEEECCCCCCCCCCCCEEECC
KWLPQEQK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2162463 [H]