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The map label for this gene is hpaG1 [H]

Identifier: 238892920

GI number: 238892920

Start: 730366

End: 730998

Strand: Reverse

Name: hpaG1 [H]

Synonym: KP1_0743

Alternate gene names: 238892920

Gene position: 730998-730366 (Counterclockwise)

Preceding gene: 238892925

Following gene: 238892919

Centisome position: 13.93

GC content: 62.56

Gene sequence:

>633_bases
ATGAAAGGTACCATCTTCGCCGTTGCGCTCAATCATCGCAGCCAGCTCGACGCCTGGCGCGAAGCGTTTCAACAGGCCCC
CTACAAGACGCCGCCGAAAACCGCGGTCTGGTTCATCAAACCCCGTAATACCGTGATTGGCGATGGCGAAGCCATTCCCC
ATCCGCAGGGCGAGACCGTACAAAGCGGCGCGACGGTGGCGCTGATTGTCGGCAAAACCGCGCGTAAAGTGGCGGCGGAA
GAGGCGGCGAACTATATCGCCGGCTACGCGCTGGCTAACGACGTTAGCCTGCCGGAAGAGAGTTTTTATCGCCCGGCGAT
CAAGGCCAAATGCCGTGATGGCTTCTGTCCCATCGGCGCGCTGTCGGCGGTGCGCAACGTCGATAACCTGACCATCATCA
CCGAAATCAACGGCCGCGAGGCGGATAGCTGGCATACCGGCGATCTGCAGCGTAGCGCCGCGCAGCTGCTGAGCGCCTTA
AGCGAGTTCGCTACCCTCAATCCCGGCGACGCGATCCTTATCGGCACGCCCCACTCCCGCGTCACGCTGCGCCCCGGCGA
CCGGGTACGTATTCTCGCCGACGGTTTCCCGGCGCTCGAAAACCCGGTTGTCGCAGAAGGAGAGCTGGCATGA

Upstream 100 bases:

>100_bases
CACTTCCAGTTGCTAAACCTCGACGCAAAGCATAAAAGATGATCATTAACATATTAATGAAATCCCTTGGAATCCAAAAT
CAGTCCCAGAGGAGTGGTGA

Downstream 100 bases:

>100_bases
AACAGGCACGCATTGAATGGCAAGGACAGGTACGCGATGTGCTGGTCAATGAACGCGACCAGGTGCGCCTGGACGATGGC
ACGGTATTAAAAGAAGGTGA

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase N-terminal subunit

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKGTIFAVALNHRSQLDAWREAFQQAPYKTPPKTAVWFIKPRNTVIGDGEAIPHPQGETVQSGATVALIVGKTARKVAAE
EAANYIAGYALANDVSLPEESFYRPAIKAKCRDGFCPIGALSAVRNVDNLTIITEINGREADSWHTGDLQRSAAQLLSAL
SEFATLNPGDAILIGTPHSRVTLRPGDRVRILADGFPALENPVVAEGELA

Sequences:

>Translated_210_residues
MKGTIFAVALNHRSQLDAWREAFQQAPYKTPPKTAVWFIKPRNTVIGDGEAIPHPQGETVQSGATVALIVGKTARKVAAE
EAANYIAGYALANDVSLPEESFYRPAIKAKCRDGFCPIGALSAVRNVDNLTIITEINGREADSWHTGDLQRSAAQLLSAL
SEFATLNPGDAILIGTPHSRVTLRPGDRVRILADGFPALENPVVAEGELA
>Mature_210_residues
MKGTIFAVALNHRSQLDAWREAFQQAPYKTPPKTAVWFIKPRNTVIGDGEAIPHPQGETVQSGATVALIVGKTARKVAAE
EAANYIAGYALANDVSLPEESFYRPAIKAKCRDGFCPIGALSAVRNVDNLTIITEINGREADSWHTGDLQRSAAQLLSAL
SEFATLNPGDAILIGTPHSRVTLRPGDRVRILADGFPALENPVVAEGELA

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=215, Percent_Identity=28.3720930232558, Blast_Score=80, Evalue=9e-16,
Organism=Homo sapiens, GI40786394, Length=188, Percent_Identity=29.2553191489362, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1787428, Length=175, Percent_Identity=30.2857142857143, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557057, Length=171, Percent_Identity=29.2397660818713, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28572127, Length=218, Percent_Identity=29.8165137614679, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24663695, Length=185, Percent_Identity=27.5675675675676, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571789, Length=168, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 22512; Mature: 22512

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGTIFAVALNHRSQLDAWREAFQQAPYKTPPKTAVWFIKPRNTVIGDGEAIPHPQGETV
CCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCC
QSGATVALIVGKTARKVAAEEAANYIAGYALANDVSLPEESFYRPAIKAKCRDGFCPIGA
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHCCHHHHHCCCCCCCHHH
LSAVRNVDNLTIITEINGREADSWHTGDLQRSAAQLLSALSEFATLNPGDAILIGTPHSR
HHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCE
VTLRPGDRVRILADGFPALENPVVAEGELA
EEECCCCEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKGTIFAVALNHRSQLDAWREAFQQAPYKTPPKTAVWFIKPRNTVIGDGEAIPHPQGETV
CCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCC
QSGATVALIVGKTARKVAAEEAANYIAGYALANDVSLPEESFYRPAIKAKCRDGFCPIGA
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHCCHHHHHCCCCCCCHHH
LSAVRNVDNLTIITEINGREADSWHTGDLQRSAAQLLSALSEFATLNPGDAILIGTPHSR
HHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCE
VTLRPGDRVRILADGFPALENPVVAEGELA
EEECCCCEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550403 [H]