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The map label for this gene is hpaG2 [H]

Identifier: 238892919

GI number: 238892919

Start: 729605

End: 730369

Strand: Reverse

Name: hpaG2 [H]

Synonym: KP1_0742

Alternate gene names: 238892919

Gene position: 730369-729605 (Counterclockwise)

Preceding gene: 238892920

Following gene: 238892918

Centisome position: 13.92

GC content: 59.08

Gene sequence:

>765_bases
ATGAAACAGGCACGCATTGAATGGCAAGGACAGGTACGCGATGTGCTGGTCAATGAACGCGACCAGGTGCGCCTGGACGA
TGGCACGGTATTAAAAGAAGGTGAATTCCGCTGGCTGCCGCCCGCCAACGGCACGCTGTTCGCGCTGGGGCTGAATTACG
CCGACCACGCCAGCGAGCTGGAATTTAAACCGCCAACCGAGCCGTTGGTGTTTATCAAAGCGCCGAACACCTTTACTGGC
CACCAGCAGCAGTCGGTACGCCCGGATAACGTCGAATACATGCACTACGAAGCCGAACTGGTGGTGGTGATTGGGAAAAC
CGCGCGCCGCGTCAGCGAAGCCGAGGCTATGGATTATGTCGCCGGCTATACCGTGTGCAATGACTACGCGATCCGCGACT
ATCTGGAAAACTACTACCGCCCGAACCTGCGGGTGAAAAGCCGCGACACCCTGACGCCGATCGGCCCGTGGATCGTCAGC
AAAGAGGCGATCCCGGATCCGCATAATCTGGCCCTGCGCACCTGGGTCAACGGCGAGCTGCGCCAGCAGGGCACCACCGC
CGATCTGATCTTCAGCATCCCGTTCCTCATCGCTTATTTAAGCGAATTTATGACCCTGCAACCGGGCGACATGATCGCCA
CCGGCACGCCGAAAGGGCTATCCGACGTGGTACCGGGCGACGAGGTGATCGTTGAAGTCGAGGGCGTGGGCCGCCTGGTC
AACCATATCATCAGCCAGCAAGCGTATGAGGAGACACTGTCATGA

Upstream 100 bases:

>100_bases
CCCACTCCCGCGTCACGCTGCGCCCCGGCGACCGGGTACGTATTCTCGCCGACGGTTTCCCGGCGCTCGAAAACCCGGTT
GTCGCAGAAGGAGAGCTGGC

Downstream 100 bases:

>100_bases
AAAAGATTAACCATTGGATCAACGGTAAAAACGTTGCCGGCGCAGACTATTTTCATACCACCAACCCGGCCACCGGCGAG
GTACTGGCGGAAGTCGCCTC

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase C-terminal subunit

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKQARIEWQGQVRDVLVNERDQVRLDDGTVLKEGEFRWLPPANGTLFALGLNYADHASELEFKPPTEPLVFIKAPNTFTG
HQQQSVRPDNVEYMHYEAELVVVIGKTARRVSEAEAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPWIVS
KEAIPDPHNLALRTWVNGELRQQGTTADLIFSIPFLIAYLSEFMTLQPGDMIATGTPKGLSDVVPGDEVIVEVEGVGRLV
NHIISQQAYEETLS

Sequences:

>Translated_254_residues
MKQARIEWQGQVRDVLVNERDQVRLDDGTVLKEGEFRWLPPANGTLFALGLNYADHASELEFKPPTEPLVFIKAPNTFTG
HQQQSVRPDNVEYMHYEAELVVVIGKTARRVSEAEAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPWIVS
KEAIPDPHNLALRTWVNGELRQQGTTADLIFSIPFLIAYLSEFMTLQPGDMIATGTPKGLSDVVPGDEVIVEVEGVGRLV
NHIISQQAYEETLS
>Mature_254_residues
MKQARIEWQGQVRDVLVNERDQVRLDDGTVLKEGEFRWLPPANGTLFALGLNYADHASELEFKPPTEPLVFIKAPNTFTG
HQQQSVRPDNVEYMHYEAELVVVIGKTARRVSEAEAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPWIVS
KEAIPDPHNLALRTWVNGELRQQGTTADLIFSIPFLIAYLSEFMTLQPGDMIATGTPKGLSDVVPGDEVIVEVEGVGRLV
NHIISQQAYEETLS

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI215422413, Length=197, Percent_Identity=41.1167512690355, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI66348062, Length=194, Percent_Identity=41.7525773195876, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI156231349, Length=225, Percent_Identity=36.4444444444444, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI13654274, Length=194, Percent_Identity=41.7525773195876, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI40786394, Length=225, Percent_Identity=36.4444444444444, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1787428, Length=193, Percent_Identity=38.860103626943, Blast_Score=136, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17557057, Length=187, Percent_Identity=38.5026737967914, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324161, Length=223, Percent_Identity=28.2511210762332, Blast_Score=85, Evalue=9e-18,
Organism=Drosophila melanogaster, GI28572127, Length=217, Percent_Identity=43.3179723502304, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24663695, Length=244, Percent_Identity=33.6065573770492, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI28571789, Length=206, Percent_Identity=38.8349514563107, Blast_Score=131, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 28637; Mature: 28637

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQARIEWQGQVRDVLVNERDQVRLDDGTVLKEGEFRWLPPANGTLFALGLNYADHASEL
CCCCCEEECCCHHHHHHCCCCEEEECCCCEEECCCEEECCCCCCEEEEEECCCCCCCCCC
EFKPPTEPLVFIKAPNTFTGHQQQSVRPDNVEYMHYEAELVVVIGKTARRVSEAEAMDYV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHH
AGYTVCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPWIVSKEAIPDPHNLALRTWVNGEL
HHHHHHCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCHH
RQQGTTADLIFSIPFLIAYLSEFMTLQPGDMIATGTPKGLSDVVPGDEVIVEVEGVGRLV
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHCCCCCEEEEEEHHHHHHH
NHIISQQAYEETLS
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKQARIEWQGQVRDVLVNERDQVRLDDGTVLKEGEFRWLPPANGTLFALGLNYADHASEL
CCCCCEEECCCHHHHHHCCCCEEEECCCCEEECCCEEECCCCCCEEEEEECCCCCCCCCC
EFKPPTEPLVFIKAPNTFTGHQQQSVRPDNVEYMHYEAELVVVIGKTARRVSEAEAMDYV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHH
AGYTVCNDYAIRDYLENYYRPNLRVKSRDTLTPIGPWIVSKEAIPDPHNLALRTWVNGEL
HHHHHHCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCHH
RQQGTTADLIFSIPFLIAYLSEFMTLQPGDMIATGTPKGLSDVVPGDEVIVEVEGVGRLV
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHCCCCCEEEEEEHHHHHHH
NHIISQQAYEETLS
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA