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The map label for this gene is thiE

Identifier: 238892468

GI number: 238892468

Start: 242842

End: 243477

Strand: Reverse

Name: thiE

Synonym: KP1_0233

Alternate gene names: 238892468

Gene position: 243477-242842 (Counterclockwise)

Preceding gene: 238892469

Following gene: 238892467

Centisome position: 4.64

GC content: 61.32

Gene sequence:

>636_bases
ATGTATCAACCTGATTTTCCACCCGTCCCGTTTCGTCTCGGACTCTACCCGGTGGTGGACAGCGTGGCGTGGATTGAGCG
CCTGCTCGAAGCTGGGGTACGTACCCTTCAGTTACGCATCAAAGACCGGCGCGACAGCGAAGTTGAGGATGACGTGATCG
CCGCCATCGCCCTTGGTCGCCGTTATCACGCCCGACTGTTCATTAACGATTACTGGCAACTGGCGATTAAGCACCAGGCC
TATGGCGTGCATCTCGGACAAGAAGATCTGGAAACCACCGATCTGAGCGCTATCCGCCAGGCCGGGCTGCGTCTTGGCGT
CTCTACTCACGATGATATGGAAATTGATGTCGCCCTTGCGGCGCGCCCCTCTTACATCGCGCTAGGTCACGTCTTCCCGA
CGCAAACCAAACAGATGCCTTCCGCGCCGCAGGGCCTCGAGCAGCTTGCCAGACATATTCAACGCCTGGCGGATTACCCC
ACCGTGGCGATCGGCGGAATTAGCCTGGAAAAGGCGCCGGGGGTACTGGCGACCGGCGTCGGCAGCATCGCGGTGGTCAG
CGCCATCACCCAGGCGGCGGACTGGCGGGCAGCGACCGACCAACTGCTGGCGCTGGCGGGAGCGGGCGATGAATGA

Upstream 100 bases:

>100_bases
ACTACGCCGCGAAGCAAAGTATCGAAGCCGGCATGGCCGATATGTCGAATAACTTCCGCGCCCGCGGCGGCGAAATCTAC
CTGAAACAGGAGGAAGCCTG

Downstream 100 bases:

>100_bases
TCACGACTTTATGCGCTATAGCCGCCAGCTGCTGCTGGAGGATATTGCCATCGAAGGGCAGCAGAAGCTGTTAGCCAGTC
GAGTGCTGATTATCGGCCTT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MYQPDFPPVPFRLGLYPVVDSVAWIERLLEAGVRTLQLRIKDRRDSEVEDDVIAAIALGRRYHARLFINDYWQLAIKHQA
YGVHLGQEDLETTDLSAIRQAGLRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHIQRLADYP
TVAIGGISLEKAPGVLATGVGSIAVVSAITQAADWRAATDQLLALAGAGDE

Sequences:

>Translated_211_residues
MYQPDFPPVPFRLGLYPVVDSVAWIERLLEAGVRTLQLRIKDRRDSEVEDDVIAAIALGRRYHARLFINDYWQLAIKHQA
YGVHLGQEDLETTDLSAIRQAGLRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHIQRLADYP
TVAIGGISLEKAPGVLATGVGSIAVVSAITQAADWRAATDQLLALAGAGDE
>Mature_211_residues
MYQPDFPPVPFRLGLYPVVDSVAWIERLLEAGVRTLQLRIKDRRDSEVEDDVIAAIALGRRYHARLFINDYWQLAIKHQA
YGVHLGQEDLETTDLSAIRQAGLRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHIQRLADYP
TVAIGGISLEKAPGVLATGVGSIAVVSAITQAADWRAATDQLLALAGAGDE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=211, Percent_Identity=89.0995260663507, Blast_Score=383, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6325042, Length=217, Percent_Identity=29.0322580645161, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_KLEP7 (A6TGQ0)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001338001.1
- ProteinModelPortal:   A6TGQ0
- STRING:   A6TGQ0
- GeneID:   5340622
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_04375
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   HAVQPSY
- ProtClustDB:   PRK03512
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23016; Mature: 23016

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQPDFPPVPFRLGLYPVVDSVAWIERLLEAGVRTLQLRIKDRRDSEVEDDVIAAIALGR
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCCHHHHHHHHHHHCC
RYHARLFINDYWQLAIKHQAYGVHLGQEDLETTDLSAIRQAGLRLGVSTHDDMEIDVALA
HHEEEEEEEHHHHEEEHHHHEEEECCCHHCCHHHHHHHHHHHHEECCCCCCCCEEEEEEE
ARPSYIALGHVFPTQTKQMPSAPQGLEQLARHIQRLADYPTVAIGGISLEKAPGVLATGV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCEECCCCCCEEHHCC
GSIAVVSAITQAADWRAATDQLLALAGAGDE
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure
MYQPDFPPVPFRLGLYPVVDSVAWIERLLEAGVRTLQLRIKDRRDSEVEDDVIAAIALGR
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCCHHHHHHHHHHHCC
RYHARLFINDYWQLAIKHQAYGVHLGQEDLETTDLSAIRQAGLRLGVSTHDDMEIDVALA
HHEEEEEEEHHHHEEEHHHHEEEECCCHHCCHHHHHHHHHHHHEECCCCCCCCEEEEEEE
ARPSYIALGHVFPTQTKQMPSAPQGLEQLARHIQRLADYPTVAIGGISLEKAPGVLATGV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCEECCCCCCEEHHCC
GSIAVVSAITQAADWRAATDQLLALAGAGDE
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA