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The map label for this gene is thiC [H]

Identifier: 238892469

GI number: 238892469

Start: 243477

End: 245372

Strand: Reverse

Name: thiC [H]

Synonym: KP1_0234

Alternate gene names: 238892469

Gene position: 245372-243477 (Counterclockwise)

Preceding gene: 238892470

Following gene: 238892468

Centisome position: 4.68

GC content: 60.39

Gene sequence:

>1896_bases
ATGTCTACTACCAAACTAACCCGTCGGGAACAACGCGAGCACGCGCAGCGATTCATTGATACGCTGGCGGGTACCGCTTT
TCCTAATTCACGCCGCATCTATGTTCACGGCTCACAAGCGGATATCCGCGTCCCGATGCGTGAAATTCAGCTCAGCCCAA
CTCTCGTCGGCGGCGATAAAGATAACCCCCGGTACGAAACCAACGAACCGATCCCGGTGTATGACACCTCCGGGCCGTAT
GGCGACCCGGATATTAGCATTGATGTCCGCCAGGGGCTGGCAAAGCTGCGCCAGCCGTGGATCGACGCGCGTAACGACTG
CGCGCCACTCAGCGAACGCAGCTCGGCCTATACCAAAGCGCGGCTGGCCGACGACGGTCTCGATGAGCTGCGCTTCAGCG
GCCTGCTGACGCCCAAACGCGCCGTCGTTGGCAAATGCGTTACCCAGCTGCACTACGCCCGCCAGGGGATAGTCACCCCG
GAGATGGAGTTCATCGCCATTCGCGAAAACATGGGTCGCGAACGTATCCGCAGCGAGGTTCTGCGCCAGCAGCACGCAGG
TGAAGGCTTCGGCGCCCACCTACCGGAAAACATCACCCCCGAGTTTGTTCGCGATGAAGTGGCCGCCGGACGCGCCATCA
TCCCCGCCAACATCAACCACCCGGAGTCTGAACCGATGATTATCGGCCGCAACTTCCTGGTGAAAGTGAATGCCAATATC
GGCAACTCGGCGGTGACCTCTTCCATCGAAGAAGAGGTCGAGAAACTGGTCTGGTCAACCCGCTGGGGCGCCGACACGGT
GATGGACCTCTCCACCGGCCGCTATATCCACGAAACCCGCGAGTGGATCCTGCGCAACAGCCCGGTGCCGATTGGTACCG
TGCCGATTTACCAGGCGCTGGAGAAGGTCAACGGCATCGCCGAGAATCTCACCTGGCAAGTCTTCCGCGATACCCTGCTG
GAACAGGCCGAGCAAGGCGTCGACTACTTCACGATTCACGCCGGCGTACTGTTGCGCTACGTCCCGATGACGGCGAAGCG
CCTGACCGGTATCGTCTCGCGCGGCGGCTCAATCATGGCTAAGTGGTGCCTGTCGCATCATCAGGAAAACTTCCTCTACC
AGCATTTCCGCGAAATCTGCGAAATCTGCGCCGCCTATGACGTGTCGCTGTCGCTGGGCGATGGCCTGCGCCCCGGCTCC
ATTCAGGATGCCAACGACGAGGCGCAGTTTTCCGAGCTGCGCACCCTCGGCGAACTGACGAAAATCGCCTGGGAATATGA
CGTGCAGGTGATGATTGAAGGCCCTGGCCACGTGCCGATGCAGATGATCCGCCGCAATATGACCGAACAGCTGGAGCACT
GCCACGAAGCGCCGTTCTACACCCTGGGGCCGCTCACCACTGATATCGCGCCGGGCTACGACCATTTCACCTCCGGTATC
GGCGCGGCAATGATCGGCTGGTTCGGCTGCGCCATGCTGTGCTATGTCACGCCGAAAGAGCATCTCGGCCTGCCCAACAA
AGAGGATGTCAAACAGGGGCTGATTACCTACAAAATCGCCGCCCACGCCGCCGACCTCGCGAAAGGCCACCCCGGGGCGC
AGATCCGCGACAACGCGATGTCAAAAGCGCGTTTTGAATTCCGCTGGGAAGACCAGTTCAACCTCGCGCTCGACCCGTTC
ACCGCGCGGGCCTATCACGATGAAACCCTGCCGCAGGAGTCCGGCAAAGTCGCCCACTTCTGCTCCATGTGCGGGCCGAA
ATTCTGTTCGATGAAAATCACTCAGGAGGTCCGCGACTACGCCGCGAAGCAAAGTATCGAAGCCGGCATGGCCGATATGT
CGAATAACTTCCGCGCCCGCGGCGGCGAAATCTACCTGAAACAGGAGGAAGCCTGA

Upstream 100 bases:

>100_bases
AAGAGTAAACTGCTGTTCGCGTCAGTCCGTCAGGGCCGATCGCATCTGTTACTCCATCCGTCGTCTGACAAGCCATGTCC
TTTTTTAACTGGAATGCGCT

Downstream 100 bases:

>100_bases
TGTATCAACCTGATTTTCCACCCGTCCCGTTTCGTCTCGGACTCTACCCGGTGGTGGACAGCGTGGCGTGGATTGAGCGC
CTGCTCGAAGCTGGGGTACG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSTTKLTRREQREHAQRFIDTLAGTAFPNSRRIYVHGSQADIRVPMREIQLSPTLVGGDKDNPRYETNEPIPVYDTSGPY
GDPDISIDVRQGLAKLRQPWIDARNDCAPLSERSSAYTKARLADDGLDELRFSGLLTPKRAVVGKCVTQLHYARQGIVTP
EMEFIAIRENMGRERIRSEVLRQQHAGEGFGAHLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAENLTWQVFRDTLL
EQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGS
IQDANDEAQFSELRTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEQLEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPF
TARAYHDETLPQESGKVAHFCSMCGPKFCSMKITQEVRDYAAKQSIEAGMADMSNNFRARGGEIYLKQEEA

Sequences:

>Translated_631_residues
MSTTKLTRREQREHAQRFIDTLAGTAFPNSRRIYVHGSQADIRVPMREIQLSPTLVGGDKDNPRYETNEPIPVYDTSGPY
GDPDISIDVRQGLAKLRQPWIDARNDCAPLSERSSAYTKARLADDGLDELRFSGLLTPKRAVVGKCVTQLHYARQGIVTP
EMEFIAIRENMGRERIRSEVLRQQHAGEGFGAHLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAENLTWQVFRDTLL
EQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGS
IQDANDEAQFSELRTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEQLEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPF
TARAYHDETLPQESGKVAHFCSMCGPKFCSMKITQEVRDYAAKQSIEAGMADMSNNFRARGGEIYLKQEEA
>Mature_630_residues
STTKLTRREQREHAQRFIDTLAGTAFPNSRRIYVHGSQADIRVPMREIQLSPTLVGGDKDNPRYETNEPIPVYDTSGPYG
DPDISIDVRQGLAKLRQPWIDARNDCAPLSERSSAYTKARLADDGLDELRFSGLLTPKRAVVGKCVTQLHYARQGIVTPE
MEFIAIRENMGRERIRSEVLRQQHAGEGFGAHLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANIG
NSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGIAENLTWQVFRDTLLE
QAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSI
QDANDEAQFSELRTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEQLEHCHEAPFYTLGPLTTDIAPGYDHFTSGIG
AAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFT
ARAYHDETLPQESGKVAHFCSMCGPKFCSMKITQEVRDYAAKQSIEAGMADMSNNFRARGGEIYLKQEEA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=631, Percent_Identity=91.283676703645, Blast_Score=1214, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 70800; Mature: 70669

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTKLTRREQREHAQRFIDTLAGTAFPNSRRIYVHGSQADIRVPMREIQLSPTLVGGDK
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECCHHHHEECCEEECCCC
DNPRYETNEPIPVYDTSGPYGDPDISIDVRQGLAKLRQPWIDARNDCAPLSERSSAYTKA
CCCCCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RLADDGLDELRFSGLLTPKRAVVGKCVTQLHYARQGIVTPEMEFIAIRENMGRERIRSEV
HHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHH
LRQQHAGEGFGAHLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCC
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
EKVNGIAENLTWQVFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFSELRTLGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KIAWEYDVQVMIEGPGHVPMQMIRRNMTEQLEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
HHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHH
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
HHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLALDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKITQEVRDY
HHCEEEEEECCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
AAKQSIEAGMADMSNNFRARGGEIYLKQEEA
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
STTKLTRREQREHAQRFIDTLAGTAFPNSRRIYVHGSQADIRVPMREIQLSPTLVGGDK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECCHHHHEECCEEECCCC
DNPRYETNEPIPVYDTSGPYGDPDISIDVRQGLAKLRQPWIDARNDCAPLSERSSAYTKA
CCCCCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RLADDGLDELRFSGLLTPKRAVVGKCVTQLHYARQGIVTPEMEFIAIRENMGRERIRSEV
HHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHH
LRQQHAGEGFGAHLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCC
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQAL
CCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
EKVNGIAENLTWQVFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KWCLSHHQENFLYQHFREICEICAAYDVSLSLGDGLRPGSIQDANDEAQFSELRTLGELT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KIAWEYDVQVMIEGPGHVPMQMIRRNMTEQLEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
HHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHH
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
HHHHHHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SKARFEFRWEDQFNLALDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKITQEVRDY
HHCEEEEEECCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
AAKQSIEAGMADMSNNFRARGGEIYLKQEEA
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA