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The map label for this gene is thiF [H]

Identifier: 238892467

GI number: 238892467

Start: 242094

End: 242849

Strand: Reverse

Name: thiF [H]

Synonym: KP1_0232

Alternate gene names: 238892467

Gene position: 242849-242094 (Counterclockwise)

Preceding gene: 238892468

Following gene: 238892466

Centisome position: 4.63

GC content: 64.95

Gene sequence:

>756_bases
ATGAATGATCACGACTTTATGCGCTATAGCCGCCAGCTGCTGCTGGAGGATATTGCCATCGAAGGGCAGCAGAAGCTGTT
AGCCAGTCGAGTGCTGATTATCGGCCTTGGCGGGCTGGGCTCCCCGGCGGCGCTCTATCTGGCCGGGGCTGGCGTCGGGA
CGCTGACCCTGGCCGATGACGACGCGGTGCATCTCAGCAACCTGCAACGGCAGATCTTGTTCACCAGCGCGGATATCGAT
CGGCCAAAAGCCGCAGCGGCACAGACCCGGCTCAGCCAGCTAAACCCGCAGATCAAACTGGTAGCGCTCCAGCAGCGCTT
AAGCGGCGAGGCGCTCCGCGCCGAAGTGGCGAAAGCGGACGTGGTGCTGGACTGTACCGATAATATGGTCACCCGCCAGG
CGATCAATGCCGCCTGTGTGGCCCTCGATACCCCGTTAGTGACCGCCAGCGCCGTGGGCTTCGGCGGTCAGCTGATGGTG
CTCACCCCGCCCTGGACGCAGGGCTGCTATCGCTGCCTGTGGCCGGAAAGCGATGAACCGCAGCGCAACTGCCGCACCGC
GGGGATCGTCGGCCCGGTGGTCGGCATGATGGGCACCCTGCAGGCGCTGGAGACAATTAAGCTGCTCAGCGGCATGGCGA
CGCCGCGCAACACCCTGCGGCTGTTCGACGCCCGCACCAGCAACTGGCGCGCCCTTGCGCTACAGCGTTCCCGGAGCTGC
CCGGTGTGCGGAGGGCGGCATGCAGATCTGGTTTAA

Upstream 100 bases:

>100_bases
GCGACCGGCGTCGGCAGCATCGCGGTGGTCAGCGCCATCACCCAGGCGGCGGACTGGCGGGCAGCGACCGACCAACTGCT
GGCGCTGGCGGGAGCGGGCG

Downstream 100 bases:

>100_bases
CGACGAGCCGCTGGCGTGCGCCGACAATCTCAGCGTCAGCGCCCTGCTGACCCAGCTCGAACAGCAGCAGCCGGGCGTTG
CGCTGGCGCTCAATCAACAC

Product: adenylyltransferase

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNDHDFMRYSRQLLLEDIAIEGQQKLLASRVLIIGLGGLGSPAALYLAGAGVGTLTLADDDAVHLSNLQRQILFTSADID
RPKAAAAQTRLSQLNPQIKLVALQQRLSGEALRAEVAKADVVLDCTDNMVTRQAINAACVALDTPLVTASAVGFGGQLMV
LTPPWTQGCYRCLWPESDEPQRNCRTAGIVGPVVGMMGTLQALETIKLLSGMATPRNTLRLFDARTSNWRALALQRSRSC
PVCGGRHADLV

Sequences:

>Translated_251_residues
MNDHDFMRYSRQLLLEDIAIEGQQKLLASRVLIIGLGGLGSPAALYLAGAGVGTLTLADDDAVHLSNLQRQILFTSADID
RPKAAAAQTRLSQLNPQIKLVALQQRLSGEALRAEVAKADVVLDCTDNMVTRQAINAACVALDTPLVTASAVGFGGQLMV
LTPPWTQGCYRCLWPESDEPQRNCRTAGIVGPVVGMMGTLQALETIKLLSGMATPRNTLRLFDARTSNWRALALQRSRSC
PVCGGRHADLV
>Mature_251_residues
MNDHDFMRYSRQLLLEDIAIEGQQKLLASRVLIIGLGGLGSPAALYLAGAGVGTLTLADDDAVHLSNLQRQILFTSADID
RPKAAAAQTRLSQLNPQIKLVALQQRLSGEALRAEVAKADVVLDCTDNMVTRQAINAACVALDTPLVTASAVGFGGQLMV
LTPPWTQGCYRCLWPESDEPQRNCRTAGIVGPVVGMMGTLQALETIKLLSGMATPRNTLRLFDARTSNWRALALQRSRSC
PVCGGRHADLV

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=251, Percent_Identity=41.0358565737052, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI4885649, Length=144, Percent_Identity=31.25, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI150417996, Length=154, Percent_Identity=29.8701298701299, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI38045948, Length=162, Percent_Identity=32.0987654320988, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI23510338, Length=154, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI23510340, Length=154, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87082356, Length=251, Percent_Identity=78.0876494023904, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=43.265306122449, Blast_Score=210, Evalue=7e-56,
Organism=Escherichia coli, GI1789177, Length=136, Percent_Identity=30.1470588235294, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=248, Percent_Identity=38.3064516129032, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI193203301, Length=154, Percent_Identity=33.1168831168831, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321903, Length=253, Percent_Identity=35.1778656126482, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320598, Length=163, Percent_Identity=30.6748466257669, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322639, Length=173, Percent_Identity=26.5895953757225, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582879, Length=251, Percent_Identity=41.4342629482072, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24660640, Length=151, Percent_Identity=31.1258278145695, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28573937, Length=171, Percent_Identity=29.8245614035088, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 26962; Mature: 26962

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDHDFMRYSRQLLLEDIAIEGQQKLLASRVLIIGLGGLGSPAALYLAGAGVGTLTLADD
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECCCCCCCEEEEECCCCEEEEECCC
DAVHLSNLQRQILFTSADIDRPKAAAAQTRLSQLNPQIKLVALQQRLSGEALRAEVAKAD
CCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHCC
VVLDCTDNMVTRQAINAACVALDTPLVTASAVGFGGQLMVLTPPWTQGCYRCLWPESDEP
EEEECCCCHHHHHHHHHHHEEECCCCCEEHCCCCCCEEEEECCCCHHHHHEECCCCCCCH
QRNCRTAGIVGPVVGMMGTLQALETIKLLSGMATPRNTLRLFDARTSNWRALALQRSRSC
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCEEEEEEECCCCC
PVCGGRHADLV
CCCCCCCCCCC
>Mature Secondary Structure
MNDHDFMRYSRQLLLEDIAIEGQQKLLASRVLIIGLGGLGSPAALYLAGAGVGTLTLADD
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECCCCCCCEEEEECCCCEEEEECCC
DAVHLSNLQRQILFTSADIDRPKAAAAQTRLSQLNPQIKLVALQQRLSGEALRAEVAKAD
CCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHCC
VVLDCTDNMVTRQAINAACVALDTPLVTASAVGFGGQLMVLTPPWTQGCYRCLWPESDEP
EEEECCCCHHHHHHHHHHHEEECCCCCEEHCCCCCCEEEEECCCCHHHHHEECCCCCCCH
QRNCRTAGIVGPVVGMMGTLQALETIKLLSGMATPRNTLRLFDARTSNWRALALQRSRSC
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCEEEEEEECCCCC
PVCGGRHADLV
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]