Click here to switch to the map view.

The map label for this gene is lgrB [H]

Identifier: 238027490

GI number: 238027490

Start: 2080843

End: 2082459

Strand: Reverse

Name: lgrB [H]

Synonym: bglu_1g19030

Alternate gene names: 238027490

Gene position: 2082459-2080843 (Counterclockwise)

Preceding gene: 238027491

Following gene: 238027489

Centisome position: 53.31

GC content: 59.25

Gene sequence:

>1617_bases
ATGACACCTCCACCCTCGAGCGACACGATCGCGACGTTCTGGCGGCGCATTCTGGCCGTCGACACACTGGCCGAGACCGA
CAACATACTCGCTAAAGGCGCGACGTCGCTGCATGTCTTGCAGTTCATCAATTGGATCGAGAAGCATTGGCATCGGCGGC
TTCCGATCAGGGCGGTCTACATGGGGCCGACGATCAGCGAGTGCGCCATGGCTGTAGCGGTGCTGGCACCTGTATCACCG
GACGATTCGGCCCGTACCGCCTACGCCGCGCCGAACACAGTTGACTTTCTGCAGGTACTGACCGGCGCGCAGCGCCACTT
TCATGCGGAAGCGCGTCGCGATCCGGCGAACCCATCTCTGTACGTCGAGGCGTCTTTCCATATCACGGGCGACGTTAATC
TCCCGGCGCTGAGGCAAAGCTTCGACATTGTGGGCCGCCGTCACGAGGCACTGCATTGCTATTTCGTGCACGAGGGCGCC
GCTGTCGAGCGACGGCTCGATCCGGATGCTCAAGCTAGCATACGCTGCCTCAACATAGACGACACTGGAGCGCCCCGCGA
TATTTCCACGCTGTCAGCCCGCCTCGCGGCGGACCTGATCGAGCAAACGATCGACCTTTCCCGTGCGCCCCTCTATAGGG
TGGTGCTGGCCGAGTTGGGACGTCGTGAATGGCTGGCCGTCATCTGTGCTCATCACATCATTTTGGATGGGCCGGGTCTG
CATCTATGGCTTAAAGAAGTATCGGACGTTTATGCAGAGATCCTTGCGACAGGAAGCGTGAGGCCTGCGCTAGCGGCCCC
ACAGCAGTCGACATATCTTGCGTGGCTCGACGCGCAACTATCCAGCGATGTCGTCGTGGACGCAGTCGACGCGTTTCGGC
AGACGATGTCGGGTGCCGAAACAACGATTTCTTTCGCTCCCGTCGAACGAACGAGCGGTGGACCAGGCCTCATGCACGGC
TCGTATAGAATTCTGCTTTCGGAAAATGCGCGCGCCGCAATAGCGGGATTTGCCGCAGATCATGGATGCAGTCACTACGC
GGCACTGATTACGGCCTGGAACATCGTGATTTATCGTCGTCTCGGCATCCAAGACATGACCTTTCGCGCGGCGGGCAGCG
CCAGAGACCACGCCGAGTTTGCTCAGATGATTGGCATGTTATGGGCGCCACTCTACGTTCGATGCCAGTTTTCATCCGAC
ACGACAGTTGGCCAGCTCGCAGGGCTCGCGCAAGCGTCCGTCATCCGGTCCCAGGCTTTTCTGCACATGCCATTTGATAC
GTTAGTCGACGCGCTACGTCCGGAGATCGACATGGAAAAAACGCCCCCGAATTTTCACTTTACGCTTCATGGCAATCGAC
TGGACCGTTCGCTGAGACTGAAAGGGGCCGAGGTCAGCCCCTTGTCTTTCGAGCGAATCGATCATGGCGCGGACATCAAG
ATCCACATGAACGACGGGGGCGACCGTTTGAGTGGTCGCCTTGTCTATCGGGCGGACAGGTTGTCGGAGTCATTCGTCAG
TGAACTGGGGCGCGAATTTTGCGGGGTGGCGGCGCGATTGCGTGATTGGGCGCAGCAGCCAATCAGCGCATGCCGGCGCA
TTTACGCTTTGGCTTGA

Upstream 100 bases:

>100_bases
CCGGCCATCTCGACGAGATGGAGACCGGACTGCGCCGAGCGTTGCACCGCGCAAACGTGCTTTCCGACACCCTTCAGAAG
GGCTCAACCGAAGGAACGAC

Downstream 100 bases:

>100_bases
ACCAGCTCGCACGGAAGAAGCGAGATCGATCACCTGCGATGCTTTTCTGCGCGGACCATAGTGTCCGCGCCGACGGAATT
TTGAGCCACCGGGGATGCGG

Product: amino acid adenylation domain-containing protein

Products: NA

Alternate protein names: ATP-dependent alanine adenylase; AlaA; Alanine activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent alanine adenylase; AlaA; Alanine activase; ATP-dependent D-valine adenylase; D-ValA; D-valine activase; Valine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTPPPSSDTIATFWRRILAVDTLAETDNILAKGATSLHVLQFINWIEKHWHRRLPIRAVYMGPTISECAMAVAVLAPVSP
DDSARTAYAAPNTVDFLQVLTGAQRHFHAEARRDPANPSLYVEASFHITGDVNLPALRQSFDIVGRRHEALHCYFVHEGA
AVERRLDPDAQASIRCLNIDDTGAPRDISTLSARLAADLIEQTIDLSRAPLYRVVLAELGRREWLAVICAHHIILDGPGL
HLWLKEVSDVYAEILATGSVRPALAAPQQSTYLAWLDAQLSSDVVVDAVDAFRQTMSGAETTISFAPVERTSGGPGLMHG
SYRILLSENARAAIAGFAADHGCSHYAALITAWNIVIYRRLGIQDMTFRAAGSARDHAEFAQMIGMLWAPLYVRCQFSSD
TTVGQLAGLAQASVIRSQAFLHMPFDTLVDALRPEIDMEKTPPNFHFTLHGNRLDRSLRLKGAEVSPLSFERIDHGADIK
IHMNDGGDRLSGRLVYRADRLSESFVSELGREFCGVAARLRDWAQQPISACRRIYALA

Sequences:

>Translated_538_residues
MTPPPSSDTIATFWRRILAVDTLAETDNILAKGATSLHVLQFINWIEKHWHRRLPIRAVYMGPTISECAMAVAVLAPVSP
DDSARTAYAAPNTVDFLQVLTGAQRHFHAEARRDPANPSLYVEASFHITGDVNLPALRQSFDIVGRRHEALHCYFVHEGA
AVERRLDPDAQASIRCLNIDDTGAPRDISTLSARLAADLIEQTIDLSRAPLYRVVLAELGRREWLAVICAHHIILDGPGL
HLWLKEVSDVYAEILATGSVRPALAAPQQSTYLAWLDAQLSSDVVVDAVDAFRQTMSGAETTISFAPVERTSGGPGLMHG
SYRILLSENARAAIAGFAADHGCSHYAALITAWNIVIYRRLGIQDMTFRAAGSARDHAEFAQMIGMLWAPLYVRCQFSSD
TTVGQLAGLAQASVIRSQAFLHMPFDTLVDALRPEIDMEKTPPNFHFTLHGNRLDRSLRLKGAEVSPLSFERIDHGADIK
IHMNDGGDRLSGRLVYRADRLSESFVSELGREFCGVAARLRDWAQQPISACRRIYALA
>Mature_537_residues
TPPPSSDTIATFWRRILAVDTLAETDNILAKGATSLHVLQFINWIEKHWHRRLPIRAVYMGPTISECAMAVAVLAPVSPD
DSARTAYAAPNTVDFLQVLTGAQRHFHAEARRDPANPSLYVEASFHITGDVNLPALRQSFDIVGRRHEALHCYFVHEGAA
VERRLDPDAQASIRCLNIDDTGAPRDISTLSARLAADLIEQTIDLSRAPLYRVVLAELGRREWLAVICAHHIILDGPGLH
LWLKEVSDVYAEILATGSVRPALAAPQQSTYLAWLDAQLSSDVVVDAVDAFRQTMSGAETTISFAPVERTSGGPGLMHGS
YRILLSENARAAIAGFAADHGCSHYAALITAWNIVIYRRLGIQDMTFRAAGSARDHAEFAQMIGMLWAPLYVRCQFSSDT
TVGQLAGLAQASVIRSQAFLHMPFDTLVDALRPEIDMEKTPPNFHFTLHGNRLDRSLRLKGAEVSPLSFERIDHGADIKI
HMNDGGDRLSGRLVYRADRLSESFVSELGREFCGVAARLRDWAQQPISACRRIYALA

Specific function: Activates the 3rd to 6th amino acids (Ala, D-Leu, Ala and D-Val) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 4th (D-Leu) and the 6th (D-Val) amino acids [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: NA

Molecular weight: Translated: 59328; Mature: 59197

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPPSSDTIATFWRRILAVDTLAETDNILAKGATSLHVLQFINWIEKHWHRRLPIRAVY
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
MGPTISECAMAVAVLAPVSPDDSARTAYAAPNTVDFLQVLTGAQRHFHAEARRDPANPSL
ECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YVEASFHITGDVNLPALRQSFDIVGRRHEALHCYFVHEGAAVERRLDPDAQASIRCLNID
EEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHHCCCCCCCCCEEEEECC
DTGAPRDISTLSARLAADLIEQTIDLSRAPLYRVVLAELGRREWLAVICAHHIILDGPGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHEECCCCH
HLWLKEVSDVYAEILATGSVRPALAAPQQSTYLAWLDAQLSSDVVVDAVDAFRQTMSGAE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC
TTISFAPVERTSGGPGLMHGSYRILLSENARAAIAGFAADHGCSHYAALITAWNIVIYRR
CEEEEECCCCCCCCCCEECCCEEEEEECCCCEEEEECHHCCCCHHHHHHHHHHHHHHHHC
LGIQDMTFRAAGSARDHAEFAQMIGMLWAPLYVRCQFSSDTTVGQLAGLAQASVIRSQAF
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHE
LHMPFDTLVDALRPEIDMEKTPPNFHFTLHGNRLDRSLRLKGAEVSPLSFERIDHGADIK
EECCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCHHHCCCCCEEE
IHMNDGGDRLSGRLVYRADRLSESFVSELGREFCGVAARLRDWAQQPISACRRIYALA
EEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPPPSSDTIATFWRRILAVDTLAETDNILAKGATSLHVLQFINWIEKHWHRRLPIRAVY
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
MGPTISECAMAVAVLAPVSPDDSARTAYAAPNTVDFLQVLTGAQRHFHAEARRDPANPSL
ECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
YVEASFHITGDVNLPALRQSFDIVGRRHEALHCYFVHEGAAVERRLDPDAQASIRCLNID
EEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCHHHHCCCCCCCCCEEEEECC
DTGAPRDISTLSARLAADLIEQTIDLSRAPLYRVVLAELGRREWLAVICAHHIILDGPGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHEECCCCH
HLWLKEVSDVYAEILATGSVRPALAAPQQSTYLAWLDAQLSSDVVVDAVDAFRQTMSGAE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC
TTISFAPVERTSGGPGLMHGSYRILLSENARAAIAGFAADHGCSHYAALITAWNIVIYRR
CEEEEECCCCCCCCCCEECCCEEEEEECCCCEEEEECHHCCCCHHHHHHHHHHHHHHHHC
LGIQDMTFRAAGSARDHAEFAQMIGMLWAPLYVRCQFSSDTTVGQLAGLAQASVIRSQAF
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHE
LHMPFDTLVDALRPEIDMEKTPPNFHFTLHGNRLDRSLRLKGAEVSPLSFERIDHGADIK
EECCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCHHHCCCCCEEE
IHMNDGGDRLSGRLVYRADRLSESFVSELGREFCGVAARLRDWAQQPISACRRIYALA
EEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA