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The map label for this gene is dusA [H]

Identifier: 238027482

GI number: 238027482

Start: 2067040

End: 2068050

Strand: Reverse

Name: dusA [H]

Synonym: bglu_1g18850

Alternate gene names: 238027482

Gene position: 2068050-2067040 (Counterclockwise)

Preceding gene: 238027483

Following gene: 238027479

Centisome position: 52.94

GC content: 64.89

Gene sequence:

>1011_bases
ATGAATTTTCAGGAAAAATCAGTTGAATCAAGGTCTCGGCGCGTGTCCGTCGCGCCGATGATGGATTGGACGGACCGCCA
TTGTCGCTCCCTGCATCGAACGATCTCGCGCCACACTTGGCTCTACACGGAGATGGTGACGACAGGCGCGCTGATGCATG
GCGACGTCGCGCGCCATCTCGCGTTCACGCCGGACGAGGCGCCCGTCGCGCTGCAATTGGGCGGCAGCGAACCCGACGAT
CTCGCGAAATCGGCCAGGCTCGGTGAGAACTGGGGCTATGACGAAATCAATCTGAATTGCGGCTGCCCGTCGGAGCGTGT
GCAGCGCGGCGCGTTCGGCGCCTGCCTGATGAACGAGCCGGGGCTCGTGGCCGATTGCGTGAAGGCGATGCGCGACGCGG
TGTCGGTGCCGGTAACGGTCAAGCACCGGATCGGCGTGGACGCCGTGGAGGAGTACGCGTTCGTGCGGGATTTCGTGGGC
ACCGTTGCCCAGGCCGGCTGCGACGTGTTCATCGTCCACGCGCGCAACGCGATCCTGAAGGGTCTTTCACCGAAGGAGAA
CCGCGAGATCCCGCCGCTCAAGTACGACTATGCCTACCGGCTCAAGCAGGACTTCCCGGACCTCGAGATCATCATCAACG
GCGGCATCACGACACTGGACGAGGTCGAGGCCCATCTGCAGCACGTCGATGGCGTGATGCTGGGCCGAGAGGCTTATCAC
AATCCCTTCGTGCTCGCGGAGGTCGATAGGCGTTTCTACGGGGCGGCCGCTGCCGCGCCGACGCGCGAGGAGATCGAGGA
GCGGCTGATCGCCTACTGCGTCGCCGAGATCGCACGCGGCACCTATCTGGGGGCGATCGTGCGGCATGCGCTCGGGCTTT
ATCGCGGCGTGCACGGCGCGCGCGGCTGGCGGCGCGTGCTGTCGGACAGCAAACGGCTGGCGCGCGGCGACGTGGCCGCG
ATTTTCGCCGAGGCGCGTACCCACCTGACGGCTCCTTCCGAAATTTTTTAA

Upstream 100 bases:

>100_bases
GCTGTCTTTGGGAGCGACATTGTGGCCTGTCGCTTCGAAATGAGCGAAAAACGAAAGACACCAAGGTCGAACCAGGCCGC
CCCAGGACGCTCGCATCATC

Downstream 100 bases:

>100_bases
AAAAGGGGCTGTACAAAGCGAAAAAGTGCCCATATAATCTTGCTTCTTCGCTGCTGAAACGAAACGGTAGCGATTCAGAA
GCAAGCAGTAACAGTGGTGG

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNFQEKSVESRSRRVSVAPMMDWTDRHCRSLHRTISRHTWLYTEMVTTGALMHGDVARHLAFTPDEAPVALQLGGSEPDD
LAKSARLGENWGYDEINLNCGCPSERVQRGAFGACLMNEPGLVADCVKAMRDAVSVPVTVKHRIGVDAVEEYAFVRDFVG
TVAQAGCDVFIVHARNAILKGLSPKENREIPPLKYDYAYRLKQDFPDLEIIINGGITTLDEVEAHLQHVDGVMLGREAYH
NPFVLAEVDRRFYGAAAAAPTREEIEERLIAYCVAEIARGTYLGAIVRHALGLYRGVHGARGWRRVLSDSKRLARGDVAA
IFAEARTHLTAPSEIF

Sequences:

>Translated_336_residues
MNFQEKSVESRSRRVSVAPMMDWTDRHCRSLHRTISRHTWLYTEMVTTGALMHGDVARHLAFTPDEAPVALQLGGSEPDD
LAKSARLGENWGYDEINLNCGCPSERVQRGAFGACLMNEPGLVADCVKAMRDAVSVPVTVKHRIGVDAVEEYAFVRDFVG
TVAQAGCDVFIVHARNAILKGLSPKENREIPPLKYDYAYRLKQDFPDLEIIINGGITTLDEVEAHLQHVDGVMLGREAYH
NPFVLAEVDRRFYGAAAAAPTREEIEERLIAYCVAEIARGTYLGAIVRHALGLYRGVHGARGWRRVLSDSKRLARGDVAA
IFAEARTHLTAPSEIF
>Mature_336_residues
MNFQEKSVESRSRRVSVAPMMDWTDRHCRSLHRTISRHTWLYTEMVTTGALMHGDVARHLAFTPDEAPVALQLGGSEPDD
LAKSARLGENWGYDEINLNCGCPSERVQRGAFGACLMNEPGLVADCVKAMRDAVSVPVTVKHRIGVDAVEEYAFVRDFVG
TVAQAGCDVFIVHARNAILKGLSPKENREIPPLKYDYAYRLKQDFPDLEIIINGGITTLDEVEAHLQHVDGVMLGREAYH
NPFVLAEVDRRFYGAAAAAPTREEIEERLIAYCVAEIARGTYLGAIVRHALGLYRGVHGARGWRRVLSDSKRLARGDVAA
IFAEARTHLTAPSEIF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=30.0847457627119, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI40807366, Length=243, Percent_Identity=30.4526748971193, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=57.8616352201258, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1789660, Length=240, Percent_Identity=27.0833333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=29.4605809128631, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=28.7735849056604, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=183, Percent_Identity=25.6830601092896, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=25.4237288135593, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24580595, Length=292, Percent_Identity=25.6849315068493, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920448, Length=292, Percent_Identity=25.6849315068493, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37314; Mature: 37314

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQEKSVESRSRRVSVAPMMDWTDRHCRSLHRTISRHTWLYTEMVTTGALMHGDVARHL
CCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTPDEAPVALQLGGSEPDDLAKSARLGENWGYDEINLNCGCPSERVQRGAFGACLMNEP
CCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHCCCCCEEECCCC
GLVADCVKAMRDAVSVPVTVKHRIGVDAVEEYAFVRDFVGTVAQAGCDVFIVHARNAILK
CHHHHHHHHHHHHHCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHC
GLSPKENREIPPLKYDYAYRLKQDFPDLEIIINGGITTLDEVEAHLQHVDGVMLGREAYH
CCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHC
NPFVLAEVDRRFYGAAAAAPTREEIEERLIAYCVAEIARGTYLGAIVRHALGLYRGVHGA
CCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RGWRRVLSDSKRLARGDVAAIFAEARTHLTAPSEIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNFQEKSVESRSRRVSVAPMMDWTDRHCRSLHRTISRHTWLYTEMVTTGALMHGDVARHL
CCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTPDEAPVALQLGGSEPDDLAKSARLGENWGYDEINLNCGCPSERVQRGAFGACLMNEP
CCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHCCCCCEEECCCC
GLVADCVKAMRDAVSVPVTVKHRIGVDAVEEYAFVRDFVGTVAQAGCDVFIVHARNAILK
CHHHHHHHHHHHHHCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHC
GLSPKENREIPPLKYDYAYRLKQDFPDLEIIINGGITTLDEVEAHLQHVDGVMLGREAYH
CCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHC
NPFVLAEVDRRFYGAAAAAPTREEIEERLIAYCVAEIARGTYLGAIVRHALGLYRGVHGA
CCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RGWRRVLSDSKRLARGDVAAIFAEARTHLTAPSEIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]