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The map label for this gene is stcE [H]

Identifier: 238027483

GI number: 238027483

Start: 2071246

End: 2072784

Strand: Reverse

Name: stcE [H]

Synonym: bglu_1g18900

Alternate gene names: 238027483

Gene position: 2072784-2071246 (Counterclockwise)

Preceding gene: 238027484

Following gene: 238027482

Centisome position: 53.06

GC content: 64.72

Gene sequence:

>1539_bases
ATGAAAGAAAAAACGAATCCTATCGGGTTGAATCTCTGCGTAGCCGCGGTGTTGTCACTGACGGCAATGTCGGCCGCGCA
TGCGCTCCTGATCGATCAGGCCACGTTCAAGCAGAACGGTGGCAATCCTGCGAACGTACCGGGGACCATCAAGGCCGCCA
ACAAGAAGCTCGCCGACCTCAGCAAGGAAGCGGCATGGCGCAGCGTCGGTAGGCTCGATCTGGCCCCGGTGAATCGCCGC
TTGCCGGGTATCTCCTGCACCGCGACGTGGATTGGCGACGCAGGCCGTTGGTCCTATTTCATGACGGCGGCGCATTGCCT
GGCCCCGCGCGGCGGTCGCCCCGAAATGCCGGTCTCGGCCCGTTTCACGGACTGGAGCGGTCGCACGGTGGCGGGTGGCC
GCGGCACGGCCTATTTCACGGACCCCAACTTCAGGCCGGGCGTGCTCGTGAAGACGCAGACGTTGGGCAATGGCGCTTCG
GGCCAGCTCGCGATCGTCAAGCTGCCGCGGCACGACTTCATCAAGGACCGCTTCCGGCTGACGCCGATCGAGCCGCCGAT
CATCAACGACATCGCGGACGAGATCGGCCGCGATACCATCTTCGTCGGCTATGGTATGTGGGGCGTTGGCCCGAACCGGA
CCGCCTTCAGCCACAACCTGGCCGTCGGCGACGGCCGCCTGTACGGCCGCAGCCGCGTCGACGGCGTCTCCAGCGACGGG
CTCGGCCTGATTGCCAACTATCAGCCGGCCGGCCCGTCGTCGCACTGGGCGCAGATCACGCCCGGCGACGACGGCGCAGC
GTGGTGGCAGATCCATGGCACGCGGCCCACCATCGTCGCGGTCACCGATGCGGGCGCCTTCAATCTGTCGGCGGCGGTAC
GTGTGGCGAAATTCACAGACTGGATCAAGTCGGTCTATCCGCAGGCGCGCTTCCTGTCTCAGGTCAAGCCGCGTGCCTGC
CTCGTCAATGCGCTGTTCGGCGTGAAATACTGCTTGAGCGAGGGCCAGGAAAGCTACAACGGCGACGGCAAGTGGACGGT
GATGTTCTCGGGCATGGACCTCGGCGTGCAGGCCGACGATGGCGTGGCGGTGCAGTTGTCGACCGACCGTCGCATGGATG
CTGTCGGCAGTGGCAAGTCGGCAGAATTCGTCGGCACGGTGGAGAGCAGCCGGCTCAAGCATGTCAAGGCCAACGACGGT
CAGTACCGCGACTTCTCGCGGCCGAACGCGATGCGCGTGAGGTCGAGCGGCAAGCCGCTCGGCTGCATCGTGAGCCTCGA
TTCGGCTCAGAAATACTGCCTGCCGGCCGGCCAACAACCAGTCCAGCAGCTACCGGCATGGATCAAGGGGCAGGACGTGT
TCGTGCAGGCTGATCAGGGCGTGGCGGTGCGGCTGTCCGACCGCCCGAACTTCGCCTATAACCGCGTGGCGACGTTCAGT
GGCACGGTTAGTCACGACAAGCTAATCAAGGTGCGCGCCGACAACGGTGAGGATCTCGATTTCTCGCGGCCGGTGTCGAT
GGAGGTGATCCAGCATTAA

Upstream 100 bases:

>100_bases
GGGCAACGTCCCTGACGGATCGGGGGGAAGAGTTGCATTTGGTAAGTCATGGTATTCCATCTTAAACAATTAGCATTGCC
AATATATTTTTGAGTGAAAT

Downstream 100 bases:

>100_bases
CACGTACTGGGCGTAGTGTAATGGCCGAGGCGGGATGTATTCCCGCCTCGGCCATTTGAGAGTAGTCACACGGCTTATTT
CCCTTCGATCGATCCACCCA

Product: peptidase S1 and S6, chymotrypsin/Hap

Products: NA

Alternate protein names: Mucinase; Neutral zinc metalloprotease stcE; Secreted protease of C1 esterase inhibitor from EHEC [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKEKTNPIGLNLCVAAVLSLTAMSAAHALLIDQATFKQNGGNPANVPGTIKAANKKLADLSKEAAWRSVGRLDLAPVNRR
LPGISCTATWIGDAGRWSYFMTAAHCLAPRGGRPEMPVSARFTDWSGRTVAGGRGTAYFTDPNFRPGVLVKTQTLGNGAS
GQLAIVKLPRHDFIKDRFRLTPIEPPIINDIADEIGRDTIFVGYGMWGVGPNRTAFSHNLAVGDGRLYGRSRVDGVSSDG
LGLIANYQPAGPSSHWAQITPGDDGAAWWQIHGTRPTIVAVTDAGAFNLSAAVRVAKFTDWIKSVYPQARFLSQVKPRAC
LVNALFGVKYCLSEGQESYNGDGKWTVMFSGMDLGVQADDGVAVQLSTDRRMDAVGSGKSAEFVGTVESSRLKHVKANDG
QYRDFSRPNAMRVRSSGKPLGCIVSLDSAQKYCLPAGQQPVQQLPAWIKGQDVFVQADQGVAVRLSDRPNFAYNRVATFS
GTVSHDKLIKVRADNGEDLDFSRPVSMEVIQH

Sequences:

>Translated_512_residues
MKEKTNPIGLNLCVAAVLSLTAMSAAHALLIDQATFKQNGGNPANVPGTIKAANKKLADLSKEAAWRSVGRLDLAPVNRR
LPGISCTATWIGDAGRWSYFMTAAHCLAPRGGRPEMPVSARFTDWSGRTVAGGRGTAYFTDPNFRPGVLVKTQTLGNGAS
GQLAIVKLPRHDFIKDRFRLTPIEPPIINDIADEIGRDTIFVGYGMWGVGPNRTAFSHNLAVGDGRLYGRSRVDGVSSDG
LGLIANYQPAGPSSHWAQITPGDDGAAWWQIHGTRPTIVAVTDAGAFNLSAAVRVAKFTDWIKSVYPQARFLSQVKPRAC
LVNALFGVKYCLSEGQESYNGDGKWTVMFSGMDLGVQADDGVAVQLSTDRRMDAVGSGKSAEFVGTVESSRLKHVKANDG
QYRDFSRPNAMRVRSSGKPLGCIVSLDSAQKYCLPAGQQPVQQLPAWIKGQDVFVQADQGVAVRLSDRPNFAYNRVATFS
GTVSHDKLIKVRADNGEDLDFSRPVSMEVIQH
>Mature_512_residues
MKEKTNPIGLNLCVAAVLSLTAMSAAHALLIDQATFKQNGGNPANVPGTIKAANKKLADLSKEAAWRSVGRLDLAPVNRR
LPGISCTATWIGDAGRWSYFMTAAHCLAPRGGRPEMPVSARFTDWSGRTVAGGRGTAYFTDPNFRPGVLVKTQTLGNGAS
GQLAIVKLPRHDFIKDRFRLTPIEPPIINDIADEIGRDTIFVGYGMWGVGPNRTAFSHNLAVGDGRLYGRSRVDGVSSDG
LGLIANYQPAGPSSHWAQITPGDDGAAWWQIHGTRPTIVAVTDAGAFNLSAAVRVAKFTDWIKSVYPQARFLSQVKPRAC
LVNALFGVKYCLSEGQESYNGDGKWTVMFSGMDLGVQADDGVAVQLSTDRRMDAVGSGKSAEFVGTVESSRLKHVKANDG
QYRDFSRPNAMRVRSSGKPLGCIVSLDSAQKYCLPAGQQPVQQLPAWIKGQDVFVQADQGVAVRLSDRPNFAYNRVATFS
GTVSHDKLIKVRADNGEDLDFSRPVSMEVIQH

Specific function: Virulence factor that contributes to intimate adherence of enterohemorrhagic E.coli (EHEC) O157:H7 to host cells. Is able to cleave the secreted human mucin 7 (MUC7) and the glycoprotein 340 (DMBT1/GP340). Also cleaves human C1 inhibitor (SERPING1), a reg

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via the etp type II secretion pathway [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase M66 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019503 [H]

Pfam domain/function: PF10462 Peptidase_M66 [H]

EC number: NA

Molecular weight: Translated: 55221; Mature: 55221

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKTNPIGLNLCVAAVLSLTAMSAAHALLIDQATFKQNGGNPANVPGTIKAANKKLADL
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECHHHHCCCCCCCCCCCEEECCCHHHHHH
SKEAAWRSVGRLDLAPVNRRLPGISCTATWIGDAGRWSYFMTAAHCLAPRGGRPEMPVSA
HHHHHHHHCCCEEECCHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEE
RFTDWSGRTVAGGRGTAYFTDPNFRPGVLVKTQTLGNGASGQLAIVKLPRHDFIKDRFRL
EEECCCCCEEECCCCEEEEECCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHCCEEE
TPIEPPIINDIADEIGRDTIFVGYGMWGVGPNRTAFSHNLAVGDGRLYGRSRVDGVSSDG
CCCCCCHHHHHHHHHCCCEEEEEECEECCCCCCCEEECEEEECCCEEECCCCCCCCCCCC
LGLIANYQPAGPSSHWAQITPGDDGAAWWQIHGTRPTIVAVTDAGAFNLSAAVRVAKFTD
CEEEEECCCCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
WIKSVYPQARFLSQVKPRACLVNALFGVKYCLSEGQESYNGDGKWTVMFSGMDLGVQADD
HHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEECCCCCEECCC
GVAVQLSTDRRMDAVGSGKSAEFVGTVESSRLKHVKANDGQYRDFSRPNAMRVRSSGKPL
CEEEEEECCCCCHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCE
GCIVSLDSAQKYCLPAGQQPVQQLPAWIKGQDVFVQADQGVAVRLSDRPNFAYNRVATFS
EEEEEECCCCCCCCCCCCCHHHHCCHHCCCCEEEEECCCCEEEEECCCCCCCEEEEEEEC
GTVSHDKLIKVRADNGEDLDFSRPVSMEVIQH
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MKEKTNPIGLNLCVAAVLSLTAMSAAHALLIDQATFKQNGGNPANVPGTIKAANKKLADL
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECHHHHCCCCCCCCCCCEEECCCHHHHHH
SKEAAWRSVGRLDLAPVNRRLPGISCTATWIGDAGRWSYFMTAAHCLAPRGGRPEMPVSA
HHHHHHHHCCCEEECCHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEE
RFTDWSGRTVAGGRGTAYFTDPNFRPGVLVKTQTLGNGASGQLAIVKLPRHDFIKDRFRL
EEECCCCCEEECCCCEEEEECCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHCCEEE
TPIEPPIINDIADEIGRDTIFVGYGMWGVGPNRTAFSHNLAVGDGRLYGRSRVDGVSSDG
CCCCCCHHHHHHHHHCCCEEEEEECEECCCCCCCEEECEEEECCCEEECCCCCCCCCCCC
LGLIANYQPAGPSSHWAQITPGDDGAAWWQIHGTRPTIVAVTDAGAFNLSAAVRVAKFTD
CEEEEECCCCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
WIKSVYPQARFLSQVKPRACLVNALFGVKYCLSEGQESYNGDGKWTVMFSGMDLGVQADD
HHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEECCCCCEECCC
GVAVQLSTDRRMDAVGSGKSAEFVGTVESSRLKHVKANDGQYRDFSRPNAMRVRSSGKPL
CEEEEEECCCCCHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCE
GCIVSLDSAQKYCLPAGQQPVQQLPAWIKGQDVFVQADQGVAVRLSDRPNFAYNRVATFS
EEEEEECCCCCCCCCCCCCHHHHCCHHCCCCEEEEECCCCEEEEECCCCCCCEEEEEEEC
GTVSHDKLIKVRADNGEDLDFSRPVSMEVIQH
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9722640; 9628576; 12123444 [H]