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The map label for this gene is gcvA [H]

Identifier: 238026857

GI number: 238026857

Start: 1335744

End: 1336616

Strand: Direct

Name: gcvA [H]

Synonym: bglu_1g12250

Alternate gene names: 238026857

Gene position: 1335744-1336616 (Clockwise)

Preceding gene: 238026856

Following gene: 238026858

Centisome position: 34.19

GC content: 67.35

Gene sequence:

>873_bases
ATGATCCTGCCGCCGTTGAACGCCCTGCGTGCCTTCGAGGCCACCGCACGCCTGAAGAGCCTGTCGCGAGCGGGCGAAGA
ACTGCACGTGACGCACGCCGCCGTGAGCGGCCAGATCAAGAAGCTCGAGGCCTGGCTCGGCCGGCGCCTGTTCGAGCGCA
CCGGGCGCGGGGCCGTGCTGACCTCGGCCGGCGAGCAATACTTCCACACGATTCAGCCGGCGTTGGCGGCGATTTCGGCA
GTGTCGCAGTCACTGAGCATCAACCGCCACCGCAAGGGCATCTCGGTGGCCTGCATCCCGTCGGTGGCCACACGCTGGCT
GATACCGGCGCTGCCGCGCTTCGCGGCGTTCCACCCCGACATCGCGATCGAGGTGTCGTATTCGCGCGCACACGAGCAGT
TCGATCCCGAGCGGCACGACGTGCTGATCACGCATCAGCACATGCCGTCGAAGAACAGCGCCCGCGCGATGCTGTTCTCG
CGCACCAGCAAGCCGGTGGCGAGCCCGCGGTATCTGGCGCAGAAGCGCTGCGACGCGCGGCTCAACCATGCCACGCTGCT
GCATGACCGATTCGTCTCGGCCTGGGATGCATGGTTCGAGGCGGCGGGCCTGCGCCCCGAGGGGCTGACCCATGGCCCCG
TCTATCAGGACTCCAACCTGCTCGCGACGGCCGTGATCGCCGGGCACGGCGTGGCGCTATGCCCCGTGGACGTGTTTCGT
CGCGAGATCGCGCGTGGCGAACTGGTGGTGCTCTCCGAGGTGTCCACGCTCGATGATCACGGCTACTACCTCGTCTACGA
CAAGGAAAGCAGCCATGCCACGCTGGCGTTCTGCGAGTGGTTCATCGCGATCTGCGCCGAGGACCGCGACTGA

Upstream 100 bases:

>100_bases
GTGGCCCGCGCATAGCTGCAATGAACGCGCGGCTCCCCCCCACGCGCGGCGCGGCGCCGCGCCGCGCGTGTCGACCGTTT
CCCGATGGAGCCATCCAGCC

Downstream 100 bases:

>100_bases
GGGCCGGCGGCGCGGCTGGACGGTTTCGCGGCTCACGCGCGCGTAGCCGCGCTCGTCGGCGCGCGCCAGCGGATAAGCGG
CGGCGGGGCGCATCGACTCG

Product: putative LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MILPPLNALRAFEATARLKSLSRAGEELHVTHAAVSGQIKKLEAWLGRRLFERTGRGAVLTSAGEQYFHTIQPALAAISA
VSQSLSINRHRKGISVACIPSVATRWLIPALPRFAAFHPDIAIEVSYSRAHEQFDPERHDVLITHQHMPSKNSARAMLFS
RTSKPVASPRYLAQKRCDARLNHATLLHDRFVSAWDAWFEAAGLRPEGLTHGPVYQDSNLLATAVIAGHGVALCPVDVFR
REIARGELVVLSEVSTLDDHGYYLVYDKESSHATLAFCEWFIAICAEDRD

Sequences:

>Translated_290_residues
MILPPLNALRAFEATARLKSLSRAGEELHVTHAAVSGQIKKLEAWLGRRLFERTGRGAVLTSAGEQYFHTIQPALAAISA
VSQSLSINRHRKGISVACIPSVATRWLIPALPRFAAFHPDIAIEVSYSRAHEQFDPERHDVLITHQHMPSKNSARAMLFS
RTSKPVASPRYLAQKRCDARLNHATLLHDRFVSAWDAWFEAAGLRPEGLTHGPVYQDSNLLATAVIAGHGVALCPVDVFR
REIARGELVVLSEVSTLDDHGYYLVYDKESSHATLAFCEWFIAICAEDRD
>Mature_290_residues
MILPPLNALRAFEATARLKSLSRAGEELHVTHAAVSGQIKKLEAWLGRRLFERTGRGAVLTSAGEQYFHTIQPALAAISA
VSQSLSINRHRKGISVACIPSVATRWLIPALPRFAAFHPDIAIEVSYSRAHEQFDPERHDVLITHQHMPSKNSARAMLFS
RTSKPVASPRYLAQKRCDARLNHATLLHDRFVSAWDAWFEAAGLRPEGLTHGPVYQDSNLLATAVIAGHGVALCPVDVFR
REIARGELVVLSEVSTLDDHGYYLVYDKESSHATLAFCEWFIAICAEDRD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=32.7645051194539, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=31.0344827586207, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788706, Length=271, Percent_Identity=28.7822878228782, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786401, Length=254, Percent_Identity=29.1338582677165, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=25.4966887417219, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32179; Mature: 32179

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPPLNALRAFEATARLKSLSRAGEELHVTHAAVSGQIKKLEAWLGRRLFERTGRGAVL
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TSAGEQYFHTIQPALAAISAVSQSLSINRHRKGISVACIPSVATRWLIPALPRFAAFHPD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCC
IAIEVSYSRAHEQFDPERHDVLITHQHMPSKNSARAMLFSRTSKPVASPRYLAQKRCDAR
EEEEEEHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
LNHATLLHDRFVSAWDAWFEAAGLRPEGLTHGPVYQDSNLLATAVIAGHGVALCPVDVFR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHEEEEHHCCCEEECCHHHHH
REIARGELVVLSEVSTLDDHGYYLVYDKESSHATLAFCEWFIAICAEDRD
HHHCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MILPPLNALRAFEATARLKSLSRAGEELHVTHAAVSGQIKKLEAWLGRRLFERTGRGAVL
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TSAGEQYFHTIQPALAAISAVSQSLSINRHRKGISVACIPSVATRWLIPALPRFAAFHPD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCC
IAIEVSYSRAHEQFDPERHDVLITHQHMPSKNSARAMLFSRTSKPVASPRYLAQKRCDAR
EEEEEEHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
LNHATLLHDRFVSAWDAWFEAAGLRPEGLTHGPVYQDSNLLATAVIAGHGVALCPVDVFR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHEEEEHHCCCEEECCHHHHH
REIARGELVVLSEVSTLDDHGYYLVYDKESSHATLAFCEWFIAICAEDRD
HHHCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]