Click here to switch to the map view.

The map label for this gene is pcaK [H]

Identifier: 238025257

GI number: 238025257

Start: 2400827

End: 2402185

Strand: Reverse

Name: pcaK [H]

Synonym: bglu_2g19290

Alternate gene names: 238025257

Gene position: 2402185-2400827 (Counterclockwise)

Preceding gene: 238025258

Following gene: 238025253

Centisome position: 84.96

GC content: 70.42

Gene sequence:

>1359_bases
ATGAATCGCAGCCCCATCGTGAACGTGCAGACGTTCATCAATGAACAGCCGTTCGGCGCGTTCCAATGGCTGATCTTCCT
GATGTGCTTCGTGATCGTGCTGCTCGACGGCTTCGACACGGCGGCGATCGGCTTCATCGCGCCGTCGCTGCTTTCCGAAT
GGCATCTGACCAAGCCCGATCTGGCGCCGGTGCTCAGCGCGGCGCTGTTCGGGCTGGCCTGCGGCGCGCTCGTTTCCGGC
CCGCTGTCGGACCGGCTCGGGCGGCGCGCGCTGTTGATCGGCTCGGTGCTGTTGTTCGGCGTGGCGTGCCTGGCCTCGGC
GTTCGCGTCGAGCATCGGCACGCTGACGACGCTGCGCTTCGTGACGGGCGTGGGCCTCGGCGCCGCGATGCCGAACGCGG
TCACGATGATGGGCGAGTTCTGCCCGGATAGGCGCCGCGCCACGCTGATCAACCTGATGTTTTGCGGCTTTCCGCTCGGC
GCCGCGTTCGGCGGCTTCCTGGCCGCCTGGATGATTCCGCAGTTCGGCTGGCGCAGCGTGCTGCTGGCGGGCGGCGTCGC
GCCGCTCGTGCTGGCCGTGGCGCTCGTCGCGCGGATGCCGGAATCGGTGCGCTTCATGGTGGCCACGCGCCGGCCCGTCG
AGCGGATTCGCGCGACGCTCGCGCGCGTGTCGGCACGCGCGCGCGAGGCCGGCTCGTTCGTGCTGACGGAGACGGTGCCG
CGCACCGAGGGTAAGGGCGCGGCGGTGGTGCTGTCGCGCCCGTACCGGGTGGGCTCGGTGATGCTGTGGATCGCCTATTT
CATGGGCCTGGTGATCTTCTACGCCTCGATCAACTGGATGCCGATCCTGCTCAAGGACGCGGGGCTCGCTCCGAAGGCGG
CCACGCTGATCTCGGCGCTATTCCCGCTCGGCGGCGTGGGCGCGGTGCTCAGCGGCGCGCTGATGGATCGCTTCAACGCG
AACCGCGTGGTGGCCGTCTGCTATGCGCTGACGGCGGTGAGCGTCTACGCGATCGGGCAGGCGGCCGGCAACGTCGGCGC
GCTGGTGGTGGTGGTGTTCCTGGCCGGCGTGCTGATGAACACGGCGCAGTCGTCGCTGCCGGCGCTGGCCGCCGCGTTCT
ATCCCACCTCGGGGCGCGGCACCGGGGTGGCCTGGATGCTCGGCATCGGCCGCTTCGGCGGGATCGCGGGCTCGTTCCTG
GTGGCCGAACTGACGGCCCGGCACGTGTCGTTCTCGGGCGTGTTCGCCACCATCGCGGTGGCGGGCCTGGTGTCGTGCGC
GGCGCTGCTGGTCAAGCAGGGCGTGCGGCCGCAGCAGGCGGCGCAAGGCGGCGAGCAGGCGGAGTCACTCGGCCATTGA

Upstream 100 bases:

>100_bases
TGTTCTGATCCGGAACCGAAGTTCGAGATACGAATTTCGCTCCGCCGAATCCGAATCGAAGCAGCGGCGCGATCCACCCG
CGCGGCGCAGGAGACCCGCG

Downstream 100 bases:

>100_bases
GCCGCGGCGCGGCCGGCCTCAGGCGTCCGCCGCCGGCTTCGGCGTGGGCGCCGGCTTGGGCGCGTGGCGCGCCAGGAAGC
GGTCGAACTGCGAGGCGAAC

Product: major facilitator superfamily transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNRSPIVNVQTFINEQPFGAFQWLIFLMCFVIVLLDGFDTAAIGFIAPSLLSEWHLTKPDLAPVLSAALFGLACGALVSG
PLSDRLGRRALLIGSVLLFGVACLASAFASSIGTLTTLRFVTGVGLGAAMPNAVTMMGEFCPDRRRATLINLMFCGFPLG
AAFGGFLAAWMIPQFGWRSVLLAGGVAPLVLAVALVARMPESVRFMVATRRPVERIRATLARVSARAREAGSFVLTETVP
RTEGKGAAVVLSRPYRVGSVMLWIAYFMGLVIFYASINWMPILLKDAGLAPKAATLISALFPLGGVGAVLSGALMDRFNA
NRVVAVCYALTAVSVYAIGQAAGNVGALVVVVFLAGVLMNTAQSSLPALAAAFYPTSGRGTGVAWMLGIGRFGGIAGSFL
VAELTARHVSFSGVFATIAVAGLVSCAALLVKQGVRPQQAAQGGEQAESLGH

Sequences:

>Translated_452_residues
MNRSPIVNVQTFINEQPFGAFQWLIFLMCFVIVLLDGFDTAAIGFIAPSLLSEWHLTKPDLAPVLSAALFGLACGALVSG
PLSDRLGRRALLIGSVLLFGVACLASAFASSIGTLTTLRFVTGVGLGAAMPNAVTMMGEFCPDRRRATLINLMFCGFPLG
AAFGGFLAAWMIPQFGWRSVLLAGGVAPLVLAVALVARMPESVRFMVATRRPVERIRATLARVSARAREAGSFVLTETVP
RTEGKGAAVVLSRPYRVGSVMLWIAYFMGLVIFYASINWMPILLKDAGLAPKAATLISALFPLGGVGAVLSGALMDRFNA
NRVVAVCYALTAVSVYAIGQAAGNVGALVVVVFLAGVLMNTAQSSLPALAAAFYPTSGRGTGVAWMLGIGRFGGIAGSFL
VAELTARHVSFSGVFATIAVAGLVSCAALLVKQGVRPQQAAQGGEQAESLGH
>Mature_452_residues
MNRSPIVNVQTFINEQPFGAFQWLIFLMCFVIVLLDGFDTAAIGFIAPSLLSEWHLTKPDLAPVLSAALFGLACGALVSG
PLSDRLGRRALLIGSVLLFGVACLASAFASSIGTLTTLRFVTGVGLGAAMPNAVTMMGEFCPDRRRATLINLMFCGFPLG
AAFGGFLAAWMIPQFGWRSVLLAGGVAPLVLAVALVARMPESVRFMVATRRPVERIRATLARVSARAREAGSFVLTETVP
RTEGKGAAVVLSRPYRVGSVMLWIAYFMGLVIFYASINWMPILLKDAGLAPKAATLISALFPLGGVGAVLSGALMDRFNA
NRVVAVCYALTAVSVYAIGQAAGNVGALVVVVFLAGVLMNTAQSSLPALAAAFYPTSGRGTGVAWMLGIGRFGGIAGSFL
VAELTARHVSFSGVFATIAVAGLVSCAALLVKQGVRPQQAAQGGEQAESLGH

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=471, Percent_Identity=24.6284501061571, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI7662270, Length=323, Percent_Identity=20.4334365325077, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI216548223, Length=254, Percent_Identity=23.2283464566929, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI213021148, Length=262, Percent_Identity=26.7175572519084, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI166064021, Length=223, Percent_Identity=29.5964125560538, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI8923870, Length=417, Percent_Identity=24.7002398081535, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081723, Length=415, Percent_Identity=33.2530120481928, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87082231, Length=461, Percent_Identity=26.8980477223427, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789207, Length=277, Percent_Identity=29.2418772563177, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788074, Length=363, Percent_Identity=26.7217630853994, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786229, Length=182, Percent_Identity=28.021978021978, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082159, Length=311, Percent_Identity=27.0096463022508, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789312, Length=214, Percent_Identity=30.8411214953271, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788068, Length=445, Percent_Identity=23.8202247191011, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32564663, Length=469, Percent_Identity=24.0938166311301, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71980582, Length=341, Percent_Identity=21.9941348973607, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71980584, Length=341, Percent_Identity=21.9941348973607, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19922874, Length=276, Percent_Identity=27.1739130434783, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640198, Length=224, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640196, Length=224, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640200, Length=224, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24658696, Length=173, Percent_Identity=28.3236994219653, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47148; Mature: 47148

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSPIVNVQTFINEQPFGAFQWLIFLMCFVIVLLDGFDTAAIGFIAPSLLSEWHLTKPD
CCCCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
LAPVLSAALFGLACGALVSGPLSDRLGRRALLIGSVLLFGVACLASAFASSIGTLTTLRF
HHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTGVGLGAAMPNAVTMMGEFCPDRRRATLINLMFCGFPLGAAFGGFLAAWMIPQFGWRSV
HHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
LLAGGVAPLVLAVALVARMPESVRFMVATRRPVERIRATLARVSARAREAGSFVLTETVP
HHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCC
RTEGKGAAVVLSRPYRVGSVMLWIAYFMGLVIFYASINWMPILLKDAGLAPKAATLISAL
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
FPLGGVGAVLSGALMDRFNANRVVAVCYALTAVSVYAIGQAAGNVGALVVVVFLAGVLMN
HCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TAQSSLPALAAAFYPTSGRGTGVAWMLGIGRFGGIAGSFLVAELTARHVSFSGVFATIAV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLVSCAALLVKQGVRPQQAAQGGEQAESLGH
HHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MNRSPIVNVQTFINEQPFGAFQWLIFLMCFVIVLLDGFDTAAIGFIAPSLLSEWHLTKPD
CCCCCEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
LAPVLSAALFGLACGALVSGPLSDRLGRRALLIGSVLLFGVACLASAFASSIGTLTTLRF
HHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTGVGLGAAMPNAVTMMGEFCPDRRRATLINLMFCGFPLGAAFGGFLAAWMIPQFGWRSV
HHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
LLAGGVAPLVLAVALVARMPESVRFMVATRRPVERIRATLARVSARAREAGSFVLTETVP
HHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCC
RTEGKGAAVVLSRPYRVGSVMLWIAYFMGLVIFYASINWMPILLKDAGLAPKAATLISAL
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
FPLGGVGAVLSGALMDRFNANRVVAVCYALTAVSVYAIGQAAGNVGALVVVVFLAGVLMN
HCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TAQSSLPALAAAFYPTSGRGTGVAWMLGIGRFGGIAGSFLVAELTARHVSFSGVFATIAV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLVSCAALLVKQGVRPQQAAQGGEQAESLGH
HHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]