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The map label for this gene is ynfL [H]

Identifier: 238024748

GI number: 238024748

Start: 1774462

End: 1775391

Strand: Direct

Name: ynfL [H]

Synonym: bglu_2g13850

Alternate gene names: 238024748

Gene position: 1774462-1775391 (Clockwise)

Preceding gene: 238024743

Following gene: 238024751

Centisome position: 62.76

GC content: 72.47

Gene sequence:

>930_bases
ATGGAATTGCGACATCTGCGGTACTTCCTCGCCGTCGCCGAGGAAGGCCAGTTCACGCGCGCCGCCGAGCGGCTGGCGAT
GCAGCAGCCGCCGCTGTCGCAGCAGATCCGGCTGCTGGAGGAGGAAATCGGCTTCGACCTGTTCGTGCGCCTGCCGCGCG
GCGTGGCGCTGACGCCGGCCGGCCGTGCCTTCGCGCAAAACGCCGGGCTCGTGCTCGACACGCTGCAGCAGGGCGTCGCC
CACGCGCGGCGGATCGCGCGCGGCGAACTCGGCGCGATCGCGGTCGGGCTCACCAGCTCGGCCGGCTTCCATCCGCTGAC
GACCGACGCGATCCGCGCGTTTAGGGCGGCCAACCCCGGCATCGAGATCGCGCTGTCGGAGCGCAACGCGGCCGAGCTGA
TCGAGGGCCTGTCGCACGGCCAGCTGCAGGTGGCGTTCTTGAGAAAGCCGGTGGATGCGCCGTCGGGCGTGGCGTTCGAG
CAGATGCTCGACGAGCCGATGGTGGCGGTGCTGCCGGTCGGGCACCGGCTGCTGGCGGCAATCGGCGATGCGCCGCCGGC
GATCCCGCTGAAGGCGCTCGAACCCGAGGCATTCATCCTGGTGCGCCGGCCCGGCGCCCCCGGCATGTATGCCGACCTGC
TCGCCGCGTGCCGGCGCCACGGCTTCGTACCGCAGGTCGCGCATGAGGTGCCGCGCATGCTGACCGGCATCCACATGGTG
GCGGCCGGCCTCGGCGTCACGCTCGTGCCGGCCTCAATGCGGCGCTACGACAACCGCAGCACGGTGTTCTGCCCGCTCAC
GGCCGACGCCGGCCTCTCGGCGCCGCTGCATCTCGCTTATCCGGCCGAGCTGCGCGATCCGGCGGCGGCCCGTTTCGTGG
CGTTCGTGCTTGCCCAGCGCGACGCGCCGGACAGGCCGGCACCCGCCTGA

Upstream 100 bases:

>100_bases
CCTAGGGAACGGCGAGCCAGATGTAAAATATTCATTCTCTGCTAGTGCCATATGTAAAAAATATGGCAAAAAATTTCGCC
CCCTGCCGGAGCCTGCCAGC

Downstream 100 bases:

>100_bases
GGCGAGCGCGGGCCGAGCCTTTGGGCCGACGGCCGGCCTCGGGGCTTGCGGCGGCAGGCACCGGTCAGCCGCGGCGCGCT
CGCGGCACACCCTGCCCGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MELRHLRYFLAVAEEGQFTRAAERLAMQQPPLSQQIRLLEEEIGFDLFVRLPRGVALTPAGRAFAQNAGLVLDTLQQGVA
HARRIARGELGAIAVGLTSSAGFHPLTTDAIRAFRAANPGIEIALSERNAAELIEGLSHGQLQVAFLRKPVDAPSGVAFE
QMLDEPMVAVLPVGHRLLAAIGDAPPAIPLKALEPEAFILVRRPGAPGMYADLLAACRRHGFVPQVAHEVPRMLTGIHMV
AAGLGVTLVPASMRRYDNRSTVFCPLTADAGLSAPLHLAYPAELRDPAAARFVAFVLAQRDAPDRPAPA

Sequences:

>Translated_309_residues
MELRHLRYFLAVAEEGQFTRAAERLAMQQPPLSQQIRLLEEEIGFDLFVRLPRGVALTPAGRAFAQNAGLVLDTLQQGVA
HARRIARGELGAIAVGLTSSAGFHPLTTDAIRAFRAANPGIEIALSERNAAELIEGLSHGQLQVAFLRKPVDAPSGVAFE
QMLDEPMVAVLPVGHRLLAAIGDAPPAIPLKALEPEAFILVRRPGAPGMYADLLAACRRHGFVPQVAHEVPRMLTGIHMV
AAGLGVTLVPASMRRYDNRSTVFCPLTADAGLSAPLHLAYPAELRDPAAARFVAFVLAQRDAPDRPAPA
>Mature_309_residues
MELRHLRYFLAVAEEGQFTRAAERLAMQQPPLSQQIRLLEEEIGFDLFVRLPRGVALTPAGRAFAQNAGLVLDTLQQGVA
HARRIARGELGAIAVGLTSSAGFHPLTTDAIRAFRAANPGIEIALSERNAAELIEGLSHGQLQVAFLRKPVDAPSGVAFE
QMLDEPMVAVLPVGHRLLAAIGDAPPAIPLKALEPEAFILVRRPGAPGMYADLLAACRRHGFVPQVAHEVPRMLTGIHMV
AAGLGVTLVPASMRRYDNRSTVFCPLTADAGLSAPLHLAYPAELRDPAAARFVAFVLAQRDAPDRPAPA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=36.426116838488, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1788887, Length=297, Percent_Identity=34.6801346801347, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI2367136, Length=296, Percent_Identity=31.7567567567568, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI157672245, Length=253, Percent_Identity=32.8063241106719, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=28.6821705426357, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=29.0196078431373, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788748, Length=251, Percent_Identity=28.6852589641434, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790208, Length=175, Percent_Identity=35.4285714285714, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=179, Percent_Identity=30.1675977653631, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787806, Length=258, Percent_Identity=28.6821705426357, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693105, Length=252, Percent_Identity=24.2063492063492, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789506, Length=252, Percent_Identity=22.6190476190476, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787589, Length=125, Percent_Identity=34.4, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33101; Mature: 33101

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFLAVAEEGQFTRAAERLAMQQPPLSQQIRLLEEEIGFDLFVRLPRGVALTPA
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCEEECCC
GRAFAQNAGLVLDTLQQGVAHARRIARGELGAIAVGLTSSAGFHPLTTDAIRAFRAANPG
CHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCC
IEIALSERNAAELIEGLSHGQLQVAFLRKPVDAPSGVAFEQMLDEPMVAVLPVGHRLLAA
EEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHH
IGDAPPAIPLKALEPEAFILVRRPGAPGMYADLLAACRRHGFVPQVAHEVPRMLTGIHMV
HCCCCCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AAGLGVTLVPASMRRYDNRSTVFCPLTADAGLSAPLHLAYPAELRDPAAARFVAFVLAQR
HHCCCCEEECHHHHHCCCCCEEEEEEECCCCCCCCEEEECCHHHCCHHHHHHHHHHHHHC
DAPDRPAPA
CCCCCCCCC
>Mature Secondary Structure
MELRHLRYFLAVAEEGQFTRAAERLAMQQPPLSQQIRLLEEEIGFDLFVRLPRGVALTPA
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCEEECCC
GRAFAQNAGLVLDTLQQGVAHARRIARGELGAIAVGLTSSAGFHPLTTDAIRAFRAANPG
CHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCC
IEIALSERNAAELIEGLSHGQLQVAFLRKPVDAPSGVAFEQMLDEPMVAVLPVGHRLLAA
EEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHH
IGDAPPAIPLKALEPEAFILVRRPGAPGMYADLLAACRRHGFVPQVAHEVPRMLTGIHMV
HCCCCCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AAGLGVTLVPASMRRYDNRSTVFCPLTADAGLSAPLHLAYPAELRDPAAARFVAFVLAQR
HHCCCCEEECHHHHHCCCCCEEEEEEECCCCCCCCEEEECCHHHCCHHHHHHHHHHHHHC
DAPDRPAPA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]