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The map label for this gene is ahpF [H]

Identifier: 238024731

GI number: 238024731

Start: 1753964

End: 1755556

Strand: Direct

Name: ahpF [H]

Synonym: bglu_2g13680

Alternate gene names: 238024731

Gene position: 1753964-1755556 (Clockwise)

Preceding gene: 238024730

Following gene: 238024732

Centisome position: 62.04

GC content: 66.98

Gene sequence:

>1593_bases
ATGCTCGACGCCAATCTCAAGAACCAGTTGAAGTCGTACCTCGACAAGATCACCCGCCCGATCGAACTCGTCGCCTCGCT
CGACAACGGCGCCAAGTCGAAGGAGCTGATCGAGCTGCTCAACGAGATCGGCTCGCTGTCGCCGCGCGTGAGCGTCGTGG
AGCGCCGCGACGACGCCGAACGCAAGCCGTCGTTCTCGATCGGCGAGCCCGGCAAGCCCACCGGCATCCGCTTCGCGGGT
ATCCCGATGGGGCATGAATTCACCTCGCTCGTGCTCGCGCTGCTGCAGGTGGGCGGCCATCCGCTCAAGCTCGACGACGC
GACGATCGAGCAGATCCGCAACCTCGAAGGCGATTTCTCCTTCGAGACGTATTTCTCGCTGAGCTGCCAGAACTGCCCGG
AAGTGGTGCAGGCGCTGAACGTGATGGCGCTGCTGAACCCGCGCATCCGCCACGTGGCGATCGACGGCGCGCTGTTCCAG
AACGAAGTGGACGAACGCCAGATCATGGCCGTGCCGACCATGTTCGTGAACGGCCAGCACTTCGGCCAGGGCCGCAGCAG
CGTCAAGGAAATCCTCGCGAAGCTCGATACCGGCGCCAGCGCCCGGGCCGCGAAGGAACTGGACAAGAAGGACGTGTTCG
ACGTGCTGATCGTCGGCGGCGGCCCGGCCGGCGCGGCGGCGGCGATCTACGCGGCGCGCAAGGGCATCCGCACCGGCGTG
GTGGCCGAGCGCTTCGGCGGCCAGGTGCTCGACACGATGGCGATCGAGAACTTCATCTCCGTGCAGGAAACCGAAGGGCC
GAAGTTCGCGGTCGCGCTGGAGCAGCACGTGAAGCAATATGACGTCGACATCATGGACGTGCAGCGCGCCACCGCGCTCG
TGCCGGGCGCGATCAACGAGATCCGCCTCGAAAACGGCGCGACGCTGAAGGCCAAGTCGGTGATCCTCTCGACGGGTGCC
CGCTGGCGCGAGCTGAACGTGCCGGGCGAGCGCGAGTACCGCAACAAGGGCGTGGCCTACTGCCCGCACTGCGACGGCCC
GCTGTTCAAGGGCAAGCGGGTGGCCGTGGTGGGCGGCGGCAACTCGGGCGTGGAAGCCGCGATCGACCTGGCCGGCATCG
TCCGTGAAGTGACGCTGATCGAATTCGGCGAGGCGCTGCGCGCCGACGAAGTGCTGCAACGCAAGCTGCGCAGCCTCAAG
AACGTGACGATCATCACCAGCGCGCAGACCAAGGAACTGCTCGGCGACGGCCAGAAGCTGAACGGCCTCGTCTACCTGGA
GCGCACGACCGGCGAAACGAAGCGCATCGAACTCGAAGGCGTGTTCGTGCAGATCGGCCTGGTGCCGAACACGGAGTGGC
TGAAGGGCACGATCGAGCTGTCGAAGCATGGCGAGATCGTGGTGGATGCGCGCGGCGCCACCTCGCTGCCGGGCGTGTTC
GCCGCCGGCGACGTGACCACGGTGCCGTACAAGCAGATCGTGATCGCGGTCGGCGAAGGCGCGAAGGCCTCGCTGTCGGC
CTTCGACCACCTGATCCGCCAGGACGTCGAGGCGCCCGCCGCCGCGCAGGCCGGCGAAGCCGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGACCGGGGCGCCGCGAGGCGCTCCGCCGTGCGGCCCTCGCGCCGCACGCCCCCTCGCCCGCCGGCGAGGTGCCAAAAAC
ATTACGGAATCCGAATCATC

Downstream 100 bases:

>100_bases
GGCGAGGCGGCCAGCCAAGCATTGCAATCGTAACGCTCTACCCTTGACCCTCGCCTGTCATGCCCCCGTGGCGGCGAGGG
TGCTTTTTCCCGACCCGCAC

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MLDANLKNQLKSYLDKITRPIELVASLDNGAKSKELIELLNEIGSLSPRVSVVERRDDAERKPSFSIGEPGKPTGIRFAG
IPMGHEFTSLVLALLQVGGHPLKLDDATIEQIRNLEGDFSFETYFSLSCQNCPEVVQALNVMALLNPRIRHVAIDGALFQ
NEVDERQIMAVPTMFVNGQHFGQGRSSVKEILAKLDTGASARAAKELDKKDVFDVLIVGGGPAGAAAAIYAARKGIRTGV
VAERFGGQVLDTMAIENFISVQETEGPKFAVALEQHVKQYDVDIMDVQRATALVPGAINEIRLENGATLKAKSVILSTGA
RWRELNVPGEREYRNKGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIVREVTLIEFGEALRADEVLQRKLRSLK
NVTIITSAQTKELLGDGQKLNGLVYLERTTGETKRIELEGVFVQIGLVPNTEWLKGTIELSKHGEIVVDARGATSLPGVF
AAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRQDVEAPAAAQAGEAVAA

Sequences:

>Translated_530_residues
MLDANLKNQLKSYLDKITRPIELVASLDNGAKSKELIELLNEIGSLSPRVSVVERRDDAERKPSFSIGEPGKPTGIRFAG
IPMGHEFTSLVLALLQVGGHPLKLDDATIEQIRNLEGDFSFETYFSLSCQNCPEVVQALNVMALLNPRIRHVAIDGALFQ
NEVDERQIMAVPTMFVNGQHFGQGRSSVKEILAKLDTGASARAAKELDKKDVFDVLIVGGGPAGAAAAIYAARKGIRTGV
VAERFGGQVLDTMAIENFISVQETEGPKFAVALEQHVKQYDVDIMDVQRATALVPGAINEIRLENGATLKAKSVILSTGA
RWRELNVPGEREYRNKGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIVREVTLIEFGEALRADEVLQRKLRSLK
NVTIITSAQTKELLGDGQKLNGLVYLERTTGETKRIELEGVFVQIGLVPNTEWLKGTIELSKHGEIVVDARGATSLPGVF
AAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRQDVEAPAAAQAGEAVAA
>Mature_530_residues
MLDANLKNQLKSYLDKITRPIELVASLDNGAKSKELIELLNEIGSLSPRVSVVERRDDAERKPSFSIGEPGKPTGIRFAG
IPMGHEFTSLVLALLQVGGHPLKLDDATIEQIRNLEGDFSFETYFSLSCQNCPEVVQALNVMALLNPRIRHVAIDGALFQ
NEVDERQIMAVPTMFVNGQHFGQGRSSVKEILAKLDTGASARAAKELDKKDVFDVLIVGGGPAGAAAAIYAARKGIRTGV
VAERFGGQVLDTMAIENFISVQETEGPKFAVALEQHVKQYDVDIMDVQRATALVPGAINEIRLENGATLKAKSVILSTGA
RWRELNVPGEREYRNKGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIVREVTLIEFGEALRADEVLQRKLRSLK
NVTIITSAQTKELLGDGQKLNGLVYLERTTGETKRIELEGVFVQIGLVPNTEWLKGTIELSKHGEIVVDARGATSLPGVF
AAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRQDVEAPAAAQAGEAVAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=517, Percent_Identity=64.2166344294004, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=316, Percent_Identity=32.2784810126582, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6321898, Length=343, Percent_Identity=30.3206997084548, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6320560, Length=312, Percent_Identity=31.0897435897436, Blast_Score=140, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56946; Mature: 56946

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANLKNQLKSYLDKITRPIELVASLDNGAKSKELIELLNEIGSLSPRVSVVERRDDAE
CCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCHH
RKPSFSIGEPGKPTGIRFAGIPMGHEFTSLVLALLQVGGHPLKLDDATIEQIRNLEGDFS
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCCCC
FETYFSLSCQNCPEVVQALNVMALLNPRIRHVAIDGALFQNEVDERQIMAVPTMFVNGQH
EEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCEEEEECEEEECCCC
FGQGRSSVKEILAKLDTGASARAAKELDKKDVFDVLIVGGGPAGAAAAIYAARKGIRTGV
CCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHCH
VAERFGGQVLDTMAIENFISVQETEGPKFAVALEQHVKQYDVDIMDVQRATALVPGAINE
HHHHHCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
IRLENGATLKAKSVILSTGARWRELNVPGEREYRNKGVAYCPHCDGPLFKGKRVAVVGGG
EEECCCCEEEEHHEEEECCCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGIVREVTLIEFGEALRADEVLQRKLRSLKNVTIITSAQTKELLGDGQKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCC
NGLVYLERTTGETKRIELEGVFVQIGLVPNTEWLKGTIELSKHGEIVVDARGATSLPGVF
CCEEEEEECCCCCEEEEEEEEEEEEEECCCHHHHEEEEEECCCCCEEEECCCCCCCCCEE
AAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRQDVEAPAAAQAGEAVAA
ECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure
MLDANLKNQLKSYLDKITRPIELVASLDNGAKSKELIELLNEIGSLSPRVSVVERRDDAE
CCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCHH
RKPSFSIGEPGKPTGIRFAGIPMGHEFTSLVLALLQVGGHPLKLDDATIEQIRNLEGDFS
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCCCC
FETYFSLSCQNCPEVVQALNVMALLNPRIRHVAIDGALFQNEVDERQIMAVPTMFVNGQH
EEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCEEEEECEEEECCCC
FGQGRSSVKEILAKLDTGASARAAKELDKKDVFDVLIVGGGPAGAAAAIYAARKGIRTGV
CCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHCH
VAERFGGQVLDTMAIENFISVQETEGPKFAVALEQHVKQYDVDIMDVQRATALVPGAINE
HHHHHCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
IRLENGATLKAKSVILSTGARWRELNVPGEREYRNKGVAYCPHCDGPLFKGKRVAVVGGG
EEECCCCEEEEHHEEEECCCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGIVREVTLIEFGEALRADEVLQRKLRSLKNVTIITSAQTKELLGDGQKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCC
NGLVYLERTTGETKRIELEGVFVQIGLVPNTEWLKGTIELSKHGEIVVDARGATSLPGVF
CCEEEEEECCCCCEEEEEEEEEEEEEECCCHHHHEEEEEECCCCCEEEECCCCCCCCCEE
AAGDVTTVPYKQIVIAVGEGAKASLSAFDHLIRQDVEAPAAAQAGEAVAA
ECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]