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The map label for this gene is queA

Identifier: 23501970

GI number: 23501970

Start: 1066752

End: 1067843

Strand: Reverse

Name: queA

Synonym: BR1092

Alternate gene names: 23501970

Gene position: 1067843-1066752 (Counterclockwise)

Preceding gene: 23501971

Following gene: 23501969

Centisome position: 50.66

GC content: 58.42

Gene sequence:

>1092_bases
ATGCGTGTTGATCTTTTCGATTTCGATCTGCCGGAGGAGCGCATTGCCTTGCGCCCGGTTGAGCCGCGCGATCATGCAAA
ACTGTTGCACGTTCGCCCCGGAGAGCCGTTTGAAGATCGACACGTCTATGATCTGCCCGACCTTTTGCAGCCGGGCGACG
CGCTTGTTTTCAATGATACCAAGGTGATACCGGCACAGCTTGAAGGAATGCGCGAGCGCACGGGCAATATTTCGCAGGTC
AGTGCAACGCTGCATATGCGTGTCGGCCCTGATCGCTGGAAGGCTTTCCTGCGCCCTGCCAAGCGCGTGAAGGAAGGCGA
CCGCATCCGCTTCGGCCATTCCGGAACAAGCTGCTTTCTCGGCACGCTCGATGCAACGGTAGCTGAAAAAGGGGATTCGG
GCGAGGCGCTTCTGGTTTTCGATCTGTCCGGTGCGGTGCTGGACGAGGCGATTGCTGCCGTCGGCCATATTCCGTTGCCA
CCCTATATTGCTTCAAAGCGCCCTGAAGACGAGCGTGACCGCAAGGACTACCAGACCGTCTATGCGCGGGAGGAAGGTGC
GGTTGCCGCACCGACGGCGGGGCTGCATTTCACGCCGGATCTTCTGGAAAAGATCAAGGCCAGGGGCATTGAGGAGCATT
TCGTAACCCTGCATGTGGGGGCGGGCACCTTTCTGCCGGTCAAGGCCGACGACACCGGCGACCACAAGATGCATGCGGAA
ATCGGCCATGTTTCGCAAAGGACGGCGAGCGCGCTCAATGCCGTGCATGAGCGGGGCGGGCGTATTATATGCGTGGGTAC
GACTTCGCTACGGCTTATCGAGAGCGCCACGGGTGAGGACGGTGTTGTGCGGCCATGGTCTGGCGCGACGGATATCTTCA
TTACGCCCGGCTATCGCTTCCGGGCTGTCGATCTGCTGATGACCAATTTTCATCTTCCGCGTTCCACCCTTTTCATGCTG
GTTTCGGCCTTCAGCGGTCTCGATACGATGCATGCTGCTTACAATTATGCGATTGCCGACGGCTACCGCTTCTATTCCTA
TGGGGACGCAAGCTTGCTTGAACGTATCGACCACGATAGACACTCGGCATGA

Upstream 100 bases:

>100_bases
ATCTCGGCTCCGGCTTCGGCCGGGGCCGACTTTATGCGTTGCGTTTCCAAACGCGATATTGCTTCGCGCTTTCGCTGGAA
ATGCCCATACGAGGTAAGTC

Downstream 100 bases:

>100_bases
CCACAGAAAATTTCGGATTCAAGGTTCTCGCGCGTGATGGCGCGGCTCGTCAGGGTGAGATTTCCATGCCGCGCGGCGTG
GTGCGCACGCCCGCCTTCAT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLPDLLQPGDALVFNDTKVIPAQLEGMRERTGNISQV
SATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFLGTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLP
PYIASKRPEDERDRKDYQTVYAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRFRAVDLLMTNFHLPRSTLFML
VSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDRHSA

Sequences:

>Translated_363_residues
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLPDLLQPGDALVFNDTKVIPAQLEGMRERTGNISQV
SATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFLGTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLP
PYIASKRPEDERDRKDYQTVYAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRFRAVDLLMTNFHLPRSTLFML
VSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDRHSA
>Mature_363_residues
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLPDLLQPGDALVFNDTKVIPAQLEGMRERTGNISQV
SATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFLGTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLP
PYIASKRPEDERDRKDYQTVYAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRFRAVDLLMTNFHLPRSTLFML
VSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDRHSA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=357, Percent_Identity=44.8179271708684, Blast_Score=266, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BRUA1 (B2S5U5)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935016.1
- ProteinModelPortal:   B2S5U5
- SMR:   B2S5U5
- GeneID:   6328584
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I10350
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40175; Mature: 40175

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLPDLLQPGDALVFNDT
CEEEEECCCCCHHHEEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCEEEECCC
KVIPAQLEGMRERTGNISQVSATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFL
EECHHHHHHHHHHCCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCEEEE
GTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLPPYIASKRPEDERDRKDYQTV
EEHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
YAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
HCCCCCCEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCEEHHH
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRF
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCEEECCCCCCEEEECCCCCE
RAVDLLMTNFHLPRSTLFMLVSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDR
EEEEEHHHCCCCCHHHHHHHHHHHCCCHHHHHHHEEEEECCEEEEEECCHHHHHHHHHCC
HSA
CCC
>Mature Secondary Structure
MRVDLFDFDLPEERIALRPVEPRDHAKLLHVRPGEPFEDRHVYDLPDLLQPGDALVFNDT
CEEEEECCCCCHHHEEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCEEEECCC
KVIPAQLEGMRERTGNISQVSATLHMRVGPDRWKAFLRPAKRVKEGDRIRFGHSGTSCFL
EECHHHHHHHHHHCCCCEEEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCEEEE
GTLDATVAEKGDSGEALLVFDLSGAVLDEAIAAVGHIPLPPYIASKRPEDERDRKDYQTV
EEHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
YAREEGAVAAPTAGLHFTPDLLEKIKARGIEEHFVTLHVGAGTFLPVKADDTGDHKMHAE
HCCCCCCEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCEEHHH
IGHVSQRTASALNAVHERGGRIICVGTTSLRLIESATGEDGVVRPWSGATDIFITPGYRF
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCEEECCCCCCEEEECCCCCE
RAVDLLMTNFHLPRSTLFMLVSAFSGLDTMHAAYNYAIADGYRFYSYGDASLLERIDHDR
EEEEEHHHCCCCCHHHHHHHHHHHCCCHHHHHHHEEEEECCEEEEEECCHHHHHHHHHCC
HSA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA