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The map label for this gene is tgt [H]

Identifier: 23501969

GI number: 23501969

Start: 1065622

End: 1066755

Strand: Reverse

Name: tgt [H]

Synonym: BR1091

Alternate gene names: 23501969

Gene position: 1066755-1065622 (Counterclockwise)

Preceding gene: 23501970

Following gene: 23501967

Centisome position: 50.61

GC content: 59.17

Gene sequence:

>1134_bases
ATGACCACAGAAAATTTCGGATTCAAGGTTCTCGCGCGTGATGGCGCGGCTCGTCAGGGTGAGATTTCCATGCCGCGCGG
CGTGGTGCGCACGCCCGCCTTCATGCCGGTCGGCACGGCTGGCACCGTGAAGGCGATGTATATGGACCAGGTGAAAGAGC
TTGGCGCCGATATCATTCTTGGCAATACCTATCACCTGATGCTGCGCCCCGGTGCCGAGCGCGTGGCCAGGCTTGGCGGC
CTGCACGAATTTGGCGGCTGGAAAGGCCCGATCCTGACTGATTCCGGCGGGTTTCAGGTCATGTCGCTTGCGCAGTTGCG
CAAGCTGAACGAGCATGGCGTTACCTTCCGTTCGCATATTGACGGCAAAGCCTATGAGATGACGCCGGAACGCTCCATTG
AAATTCAGGGGCTGCTGGATTCCGATATCCAGATGCAGCTTGATGAATGCGTGGCGCTGCCGTCACCAGAAAAGAATACC
GAACGCGCCATGGAGCTTTCGCTGCGCTGGGCCGAGCGTTGCAAGGTGGCATTTGGCGACCAGCCGGGCAAGGCCATGTT
CGGCATCGTGCAGGGGGGCGATATCGCGCGTCTGCGCGAAAGATCGGCAGAAGCGCTCAAGGCCATGGACCTCAAGGGCT
ATTCGGTTGGTGGGCTTGCGGTGGGCGAGCCGCAGGAAGTCATGCTGGATATGCTGGAAGTGGTCTGCCCGATCCTGCCA
ACGGAAAAGCCGCGCTACCTGATGGGGGTCGGCACGCCGGATGATATTTTGAAGTCCGTGGCGCGCGGGATCGATATGTT
CGATTGCGTGATGCCGACGCGCGCGGGGCGCCATGGCCTTGCTTTCACCCGCTTCGGCAAGGTGAATTTGCGCAATGCGC
GTCATGCGGAAGATCATCGCCCGCTCGATCCGCAATCCGATTGTCCGGCATCGCGCGATTATAGCCGCGCCTATCTGCAT
CATCTGGTGAAGTCCGGCGAGGCGCTCGGTGCAATGCTGCTCACCTGGAACAATCTTGCCTATTATCAATATCTCATGAA
GGGCATTCGCGCGGCGATTGCCGATGGAAATTTCAGTGATTTTACCGCTGAAACCACGGAAGGCTGGGCAAGAGGCGATA
TGCCTGCACTCTAA

Upstream 100 bases:

>100_bases
TGCATGCTGCTTACAATTATGCGATTGCCGACGGCTACCGCTTCTATTCCTATGGGGACGCAAGCTTGCTTGAACGTATC
GACCACGATAGACACTCGGC

Downstream 100 bases:

>100_bases
AACGGTTCGTGTTTTAACGGAAACGCCGGAACCGCTCTATGTCTTGGTTTCTACGCATTGTCCGCTCGTCAGCATGGTTT
CCAGGCGGCAGAGCACGGAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTTENFGFKVLARDGAARQGEISMPRGVVRTPAFMPVGTAGTVKAMYMDQVKELGADIILGNTYHLMLRPGAERVARLGG
LHEFGGWKGPILTDSGGFQVMSLAQLRKLNEHGVTFRSHIDGKAYEMTPERSIEIQGLLDSDIQMQLDECVALPSPEKNT
ERAMELSLRWAERCKVAFGDQPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVVCPILP
TEKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARHAEDHRPLDPQSDCPASRDYSRAYLH
HLVKSGEALGAMLLTWNNLAYYQYLMKGIRAAIADGNFSDFTAETTEGWARGDMPAL

Sequences:

>Translated_377_residues
MTTENFGFKVLARDGAARQGEISMPRGVVRTPAFMPVGTAGTVKAMYMDQVKELGADIILGNTYHLMLRPGAERVARLGG
LHEFGGWKGPILTDSGGFQVMSLAQLRKLNEHGVTFRSHIDGKAYEMTPERSIEIQGLLDSDIQMQLDECVALPSPEKNT
ERAMELSLRWAERCKVAFGDQPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVVCPILP
TEKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARHAEDHRPLDPQSDCPASRDYSRAYLH
HLVKSGEALGAMLLTWNNLAYYQYLMKGIRAAIADGNFSDFTAETTEGWARGDMPAL
>Mature_376_residues
TTENFGFKVLARDGAARQGEISMPRGVVRTPAFMPVGTAGTVKAMYMDQVKELGADIILGNTYHLMLRPGAERVARLGGL
HEFGGWKGPILTDSGGFQVMSLAQLRKLNEHGVTFRSHIDGKAYEMTPERSIEIQGLLDSDIQMQLDECVALPSPEKNTE
RAMELSLRWAERCKVAFGDQPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVVCPILPT
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARHAEDHRPLDPQSDCPASRDYSRAYLHH
LVKSGEALGAMLLTWNNLAYYQYLMKGIRAAIADGNFSDFTAETTEGWARGDMPAL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=345, Percent_Identity=44.0579710144928, Blast_Score=298, Evalue=7e-81,
Organism=Homo sapiens, GI13375872, Length=290, Percent_Identity=24.8275862068966, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1786607, Length=371, Percent_Identity=52.2911051212938, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17544680, Length=358, Percent_Identity=41.340782122905, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI25146502, Length=162, Percent_Identity=28.3950617283951, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19920510, Length=359, Percent_Identity=45.6824512534819, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI21355311, Length=254, Percent_Identity=25.1968503937008, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41452; Mature: 41321

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTENFGFKVLARDGAARQGEISMPRGVVRTPAFMPVGTAGTVKAMYMDQVKELGADIIL
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
GNTYHLMLRPGAERVARLGGLHEFGGWKGPILTDSGGFQVMSLAQLRKLNEHGVTFRSHI
CCEEEEEECCCHHHHHHHCCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECC
DGKAYEMTPERSIEIQGLLDSDIQMQLDECVALPSPEKNTERAMELSLRWAERCKVAFGD
CCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHECCCC
QPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVVCPILP
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHCCCC
TEKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARHAEDHR
CCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEECCEECCCCCCCCCCC
PLDPQSDCPASRDYSRAYLHHLVKSGEALGAMLLTWNNLAYYQYLMKGIRAAIADGNFSD
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHEEEEEEECCHHHHHHHHHHHHHHHCCCCCCC
FTAETTEGWARGDMPAL
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTENFGFKVLARDGAARQGEISMPRGVVRTPAFMPVGTAGTVKAMYMDQVKELGADIIL
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
GNTYHLMLRPGAERVARLGGLHEFGGWKGPILTDSGGFQVMSLAQLRKLNEHGVTFRSHI
CCEEEEEECCCHHHHHHHCCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECC
DGKAYEMTPERSIEIQGLLDSDIQMQLDECVALPSPEKNTERAMELSLRWAERCKVAFGD
CCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHECCCC
QPGKAMFGIVQGGDIARLRERSAEALKAMDLKGYSVGGLAVGEPQEVMLDMLEVVCPILP
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHCCCC
TEKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAFTRFGKVNLRNARHAEDHR
CCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEECCEECCCCCCCCCCC
PLDPQSDCPASRDYSRAYLHHLVKSGEALGAMLLTWNNLAYYQYLMKGIRAAIADGNFSD
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHEEEEEEECCHHHHHHHHHHHHHHHCCCCCCC
FTAETTEGWARGDMPAL
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]