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The map label for this gene is ilvC

Identifier: 23100076

GI number: 23100076

Start: 2684727

End: 2685761

Strand: Reverse

Name: ilvC

Synonym: OB2621

Alternate gene names: 23100076

Gene position: 2685761-2684727 (Counterclockwise)

Preceding gene: 23100077

Following gene: 23100075

Centisome position: 73.98

GC content: 40.0

Gene sequence:

>1035_bases
ATGGCAAAAGTATTATATGAAAAAGACATTCAAGACGGAGTATTGAAAAATAAGAAGATTGCAGTTATCGGTTATGGTTC
ACAAGGGCATGCCCATGCAATGAACTTAAGGGATTCTGGATATGATGTTGTCGTTGGATTACGTCCAGGGAAGTCCCAGG
AAAAAGCAGAAGAAGACGGATTTAATGTGTTGTCTGTAGCAGAGGCAAGTAGTCAGGCGGATGTTGTGATGGTACTGCTT
CCGGATGAAATGCAGCCAAAAGTATATGAAGAGAGCATTAAAGATAATCTTGAACAAGGGAACGCACTTGTATTTGCACA
TGGATTTAATATTCATTTCACACAAGTTGTACCTCCAGCAAATGTGGATGTATTCTTAGTTGCTCCAAAAGGACCTGGAC
ATTTGGTTCGTCGTACTTTTGAAGAGGGAGCTGGAGTACCAGCTTTATATGGTGTACATCAAGATTATACTGGTGAGGCA
AAAGATGTAGCACTAGCTTATTCAAAAGGAATTGGCGCTGCAAGAGCAGGGGTTCTCGAAACAAGCTTTCAAGAAGAAAC
GGAGACAGATTTATTCGGTGAGCAGGCAGTATTATGTGGAGGAGTTACTAGTCTAATCAAAGCTGGTTTTGAAACTTTGA
CAGATGCTGGATATCAACCAGAAGTAGCATACTTTGAATGTCTTCATGAAATGAAATTAATTGTTGATCTTCTTTATGAA
GGCGGATTGGAAAATATGCGTTACTCCATTTCGGATACAGCACAATGGGGAGATTTTGTATCAGGTCAGCGTGTGGTAGA
TGATCAAACAAAAGAGCGTATGAAATCTATTCTTGATGATATTCAAACCGGGTCATTTGCTAAAGGGTGGATTTTAGAGA
ATCAGGCTGGCAGACCACAATTTAACGCGATTAATCGTAGGGAAAATAGACACCCAATTGAAACTGTGGGAAGGGAGTTA
CGTGAGTTAATGCCATTTGTTAAACAGCCAATTAATGCTAAGAAAAAGGATGTGAACGTCCATGTCCCGAATTAA

Upstream 100 bases:

>100_bases
GCCAGAACAATCGATCAGTGATTTCAATCTAGTGGCGAAAGGTTAATTTCGAAAATTGATTCACAATATAAAATAATTAT
AAACGGAGAGGGAGATTAAA

Downstream 100 bases:

>100_bases
GATTTTCGACACAACACTACGAGATGGAGAGCAATCTCCAGGAGTTAACTTGAATAAAGCAGAAAAGCTTGAGATAGCAA
AACAACTTGAAAAGTATGGT

Product: ketol-acid reductoisomerase

Products: NA

Alternate protein names: Acetohydroxy-acid isomeroreductase; Alpha-keto-beta-hydroxylacil reductoisomerase

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAKVLYEKDIQDGVLKNKKIAVIGYGSQGHAHAMNLRDSGYDVVVGLRPGKSQEKAEEDGFNVLSVAEASSQADVVMVLL
PDEMQPKVYEESIKDNLEQGNALVFAHGFNIHFTQVVPPANVDVFLVAPKGPGHLVRRTFEEGAGVPALYGVHQDYTGEA
KDVALAYSKGIGAARAGVLETSFQEETETDLFGEQAVLCGGVTSLIKAGFETLTDAGYQPEVAYFECLHEMKLIVDLLYE
GGLENMRYSISDTAQWGDFVSGQRVVDDQTKERMKSILDDIQTGSFAKGWILENQAGRPQFNAINRRENRHPIETVGREL
RELMPFVKQPINAKKKDVNVHVPN

Sequences:

>Translated_344_residues
MAKVLYEKDIQDGVLKNKKIAVIGYGSQGHAHAMNLRDSGYDVVVGLRPGKSQEKAEEDGFNVLSVAEASSQADVVMVLL
PDEMQPKVYEESIKDNLEQGNALVFAHGFNIHFTQVVPPANVDVFLVAPKGPGHLVRRTFEEGAGVPALYGVHQDYTGEA
KDVALAYSKGIGAARAGVLETSFQEETETDLFGEQAVLCGGVTSLIKAGFETLTDAGYQPEVAYFECLHEMKLIVDLLYE
GGLENMRYSISDTAQWGDFVSGQRVVDDQTKERMKSILDDIQTGSFAKGWILENQAGRPQFNAINRRENRHPIETVGREL
RELMPFVKQPINAKKKDVNVHVPN
>Mature_343_residues
AKVLYEKDIQDGVLKNKKIAVIGYGSQGHAHAMNLRDSGYDVVVGLRPGKSQEKAEEDGFNVLSVAEASSQADVVMVLLP
DEMQPKVYEESIKDNLEQGNALVFAHGFNIHFTQVVPPANVDVFLVAPKGPGHLVRRTFEEGAGVPALYGVHQDYTGEAK
DVALAYSKGIGAARAGVLETSFQEETETDLFGEQAVLCGGVTSLIKAGFETLTDAGYQPEVAYFECLHEMKLIVDLLYEG
GLENMRYSISDTAQWGDFVSGQRVVDDQTKERMKSILDDIQTGSFAKGWILENQAGRPQFNAINRRENRHPIETVGRELR
ELMPFVKQPINAKKKDVNVHVPN

Specific function: Valine and isoleucine biosynthesis; second step. [C]

COG id: COG0059

COG function: function code EH; Ketol-acid reductoisomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ketol-acid reductoisomerase family

Homologues:

Organism=Escherichia coli, GI1790210, Length=291, Percent_Identity=35.3951890034364, Blast_Score=145, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323387, Length=324, Percent_Identity=33.9506172839506, Blast_Score=147, Evalue=3e-36,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ILVC_OCEIH (Q8EN66)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693542.1
- ProteinModelPortal:   Q8EN66
- SMR:   Q8EN66
- GeneID:   1015262
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2621
- NMPDR:   fig|221109.1.peg.2620
- HOGENOM:   HBG286525
- OMA:   MAYAKGI
- ProtClustDB:   PRK05479
- BioCyc:   OIHE221109:OB2621-MONOMER
- BRENDA:   1.1.1.86
- HAMAP:   MF_00435
- InterPro:   IPR008927
- InterPro:   IPR013023
- InterPro:   IPR000506
- InterPro:   IPR013328
- InterPro:   IPR013116
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR21371
- TIGRFAMs:   TIGR00465

Pfam domain/function: PF01450 IlvC; PF07991 IlvN; SSF48179 6DGDH_C_like

EC number: =1.1.1.86

Molecular weight: Translated: 37883; Mature: 37752

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: ACT_SITE 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVLYEKDIQDGVLKNKKIAVIGYGSQGHAHAMNLRDSGYDVVVGLRPGKSQEKAEEDG
CCCCHHHHCCHHHHHCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHCC
FNVLSVAEASSQADVVMVLLPDEMQPKVYEESIKDNLEQGNALVFAHGFNIHFTQVVPPA
CCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCC
NVDVFLVAPKGPGHLVRRTFEEGAGVPALYGVHQDYTGEAKDVALAYSKGIGAARAGVLE
CCEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCCHHHEEEEHHCCCCHHHHHHHH
TSFQEETETDLFGEQAVLCGGVTSLIKAGFETLTDAGYQPEVAYFECLHEMKLIVDLLYE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
GGLENMRYSISDTAQWGDFVSGQRVVDDQTKERMKSILDDIQTGSFAKGWILENQAGRPQ
CCCCCCEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCC
FNAINRRENRHPIETVGRELRELMPFVKQPINAKKKDVNVHVPN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCC
>Mature Secondary Structure 
AKVLYEKDIQDGVLKNKKIAVIGYGSQGHAHAMNLRDSGYDVVVGLRPGKSQEKAEEDG
CCCHHHHCCHHHHHCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHCC
FNVLSVAEASSQADVVMVLLPDEMQPKVYEESIKDNLEQGNALVFAHGFNIHFTQVVPPA
CCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCC
NVDVFLVAPKGPGHLVRRTFEEGAGVPALYGVHQDYTGEAKDVALAYSKGIGAARAGVLE
CCEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCCHHHEEEEHHCCCCHHHHHHHH
TSFQEETETDLFGEQAVLCGGVTSLIKAGFETLTDAGYQPEVAYFECLHEMKLIVDLLYE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
GGLENMRYSISDTAQWGDFVSGQRVVDDQTKERMKSILDDIQTGSFAKGWILENQAGRPQ
CCCCCCEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCC
FNAINRRENRHPIETVGRELRELMPFVKQPINAKKKDVNVHVPN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376