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The map label for this gene is rph

Identifier: 23099561

GI number: 23099561

Start: 2140367

End: 2141101

Strand: Reverse

Name: rph

Synonym: OB2106

Alternate gene names: 23099561

Gene position: 2141101-2140367 (Counterclockwise)

Preceding gene: 23099562

Following gene: 23099560

Centisome position: 58.97

GC content: 37.14

Gene sequence:

>735_bases
ATGAGAAATGATCAGAGAGAAGTAAATCAATTAAGAAATATAAATATAACAACTAATTATATATCGCATCCAGAGGGATC
TGTTTTAATCGAAATGGGTAATACAAAAGTTATATGTAATGCAAGTATTGAGGATCGCGTTCCACCATTTATGCGTGGAC
AGGGTAAAGGTTGGATAACTGCGGAATATGCTATGCTACCAAGAGCTACCGCACAAAGAAATATTCGTGAATCTTCCAAA
GGAAAAGTAAGCGGAAGAACGATGGAAATTCAACGCCTTATTGGTAGAGCACTACGTTCCGTTGTTGATTTAGATCAAAT
TGGAGAGCGAACAGTCTGGATTGACTGTGATGTGATTCAAGCGGACGGAGGAACTAGAACAGCTTCAATTACAGGAGCTT
TTGTGGCGATGTCACTTGCATTCGCAAAACTTGTTGAAGCCAAAACATTAAAAAAGACGCCAATTCAAGATTATTTAGCA
GCTATTTCTGTAGGCGTATTAACAAACGGAACGGAAATTCTAGATTTGAACTATGAAGAAGATTCCGAAGCAGCAGTAGA
TATGAATATTGTCATGACTGGTGAAGGGGAGTTCGTGGAAATTCAAGGAACAGGAGAAGAAGCTACATTTACTCCTAACC
AATTACAGAATATGCTAAAATTAGGAGAAGAAGGTATTCAACAGTTGGTGAAAATACAAAAGGAACTATTAGCAGATAAA
TTACTTATAGATTGA

Upstream 100 bases:

>100_bases
TTTAATTTGTTAAGATATGAATAGTAAGATATAGGGATAATGTTTCTTTTTGAAAAAATATGATAAGCTATTGAGTGTAC
AATTGAGGAGGAAGAAATAA

Downstream 100 bases:

>100_bases
AAAGTGAATGATAAGGGTGTGCTCAATCATATGAAAAAAATAATTGTTGCTACTAAAAATAAAGGAAAGGCAAAAGAATT
TAAAGAATTTTTTGCTAGCT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRNDQREVNQLRNINITTNYISHPEGSVLIEMGNTKVICNASIEDRVPPFMRGQGKGWITAEYAMLPRATAQRNIRESSK
GKVSGRTMEIQRLIGRALRSVVDLDQIGERTVWIDCDVIQADGGTRTASITGAFVAMSLAFAKLVEAKTLKKTPIQDYLA
AISVGVLTNGTEILDLNYEEDSEAAVDMNIVMTGEGEFVEIQGTGEEATFTPNQLQNMLKLGEEGIQQLVKIQKELLADK
LLID

Sequences:

>Translated_244_residues
MRNDQREVNQLRNINITTNYISHPEGSVLIEMGNTKVICNASIEDRVPPFMRGQGKGWITAEYAMLPRATAQRNIRESSK
GKVSGRTMEIQRLIGRALRSVVDLDQIGERTVWIDCDVIQADGGTRTASITGAFVAMSLAFAKLVEAKTLKKTPIQDYLA
AISVGVLTNGTEILDLNYEEDSEAAVDMNIVMTGEGEFVEIQGTGEEATFTPNQLQNMLKLGEEGIQQLVKIQKELLADK
LLID
>Mature_244_residues
MRNDQREVNQLRNINITTNYISHPEGSVLIEMGNTKVICNASIEDRVPPFMRGQGKGWITAEYAMLPRATAQRNIRESSK
GKVSGRTMEIQRLIGRALRSVVDLDQIGERTVWIDCDVIQADGGTRTASITGAFVAMSLAFAKLVEAKTLKKTPIQDYLA
AISVGVLTNGTEILDLNYEEDSEAAVDMNIVMTGEGEFVEIQGTGEEATFTPNQLQNMLKLGEEGIQQLVKIQKELLADK
LLID

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=59.7156398104265, Blast_Score=258, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_OCEIH (Q8EPJ5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693027.1
- ProteinModelPortal:   Q8EPJ5
- SMR:   Q8EPJ5
- GeneID:   1015647
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2106
- NMPDR:   fig|221109.1.peg.2104
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   OIHE221109:OB2106-MONOMER
- BRENDA:   2.7.7.56
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26916; Mature: 26916

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNDQREVNQLRNINITTNYISHPEGSVLIEMGNTKVICNASIEDRVPPFMRGQGKGWIT
CCCHHHHHHHHHCCEEEEEEECCCCCCEEEEECCEEEEEECCHHHCCCHHHCCCCCCEEE
AEYAMLPRATAQRNIRESSKGKVSGRTMEIQRLIGRALRSVVDLDQIGERTVWIDCDVIQ
EHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHCCCEEEEEEEEEEE
ADGGTRTASITGAFVAMSLAFAKLVEAKTLKKTPIQDYLAAISVGVLTNGTEILDLNYEE
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCEEEEECCCC
DSEAAVDMNIVMTGEGEFVEIQGTGEEATFTPNQLQNMLKLGEEGIQQLVKIQKELLADK
CCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLID
HCCC
>Mature Secondary Structure
MRNDQREVNQLRNINITTNYISHPEGSVLIEMGNTKVICNASIEDRVPPFMRGQGKGWIT
CCCHHHHHHHHHCCEEEEEEECCCCCCEEEEECCEEEEEECCHHHCCCHHHCCCCCCEEE
AEYAMLPRATAQRNIRESSKGKVSGRTMEIQRLIGRALRSVVDLDQIGERTVWIDCDVIQ
EHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHCCCEEEEEEEEEEE
ADGGTRTASITGAFVAMSLAFAKLVEAKTLKKTPIQDYLAAISVGVLTNGTEILDLNYEE
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCEEEEECCCC
DSEAAVDMNIVMTGEGEFVEIQGTGEEATFTPNQLQNMLKLGEEGIQQLVKIQKELLADK
CCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLID
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376