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The map label for this gene is hemB [H]

Identifier: 23099521

GI number: 23099521

Start: 2093487

End: 2094473

Strand: Reverse

Name: hemB [H]

Synonym: OB2066

Alternate gene names: 23099521

Gene position: 2094473-2093487 (Counterclockwise)

Preceding gene: 23099522

Following gene: 23099520

Centisome position: 57.69

GC content: 37.18

Gene sequence:

>987_bases
ATGGAACAACAGTTGACTTTTAAACGGCATCGTCGATTACGTGCATCTACATCTATGCGTTCATTAGTACGAGAAACGCA
TTTAAGACCTACTGATTTTATTTATCCTATTTTTGTAATAGAAGGCTCAAACATAAAAAATGAAATACCTTCAATGCCAG
GGATTTATCAGTTATCCTTTGATCATCTATTAGAAGAAGTTCAAGAAGCCTATGATTTAGGTATACGTTCTATTATTTTA
TTTGGTGTAGTGTCTAATAAAGATGAGATAGGATCAGAAGCTTTTCATGATCACGGGATTATCCAAGAGGCTACACGTAT
GGTTAAAAAAGACTTTCCAGATATGCTAGTAGTAGCAGATACTTGTTTATGCGAGTACACTTCTCATGGTCACTGTGGCG
TGATACAGAACCGCGATGTGGATAATGATGCTTCATTAGAATTATTAGCTAAAACTGCTGTTTCACAAGCTAAAGCTGGT
GCAGATATTATTGCGCCATCCAATATGATGGATGGATTTGTCGTAGTGATTCGTCAAGCTTTAGATGAAGCAGGTTTTAA
TCATATTCCAATTATGTCGTATGCAGTAAAGTATGCTTCTGCATTTTATGGTCCATTTAGAGATGCTGCTGATAGCACAC
CTCAATTTGGAGACCGTAAAACGTATCAAATGGATCCTGCAAATCGCTTAGAAGCTATTCGCGAGGCAACTTCAGACGTA
GAAGAAGGTGCTGATTTCTTAATTGTGAAGCCAGCTCTGTCTTATCTTGATATTGTTCGTGAGGTTCGTGATAACTTTGA
TTTGCCGGTAGTAGCGTATAATGTTAGTGGAGAGTATTCCATGGTAAAAGCTGCTGCACAAAATGGATGGATTGATGAAA
GAGCATTAGTAATGGAAAAACTAACATCGATGAAACGAGCAGGATCTGATTTGATTATTTCTTATTTTGCTAAAGATGTT
GCGAAATGGTTACAGGAATCGAACTAA

Upstream 100 bases:

>100_bases
AAATTACAAATACCAAATCTTCATATTCCTAGTGAATTTACTACAGATGCAATGATAGAAGTTATGATTGATTTGGCTTT
AGGAAAGGATGTAACTTAAT

Downstream 100 bases:

>100_bases
GGAGGTATTTTAATGACACTTAATCAGTCAATTGATGCGTATAAAGAAGCTGTTGATTTAATGCCGGGTGGGGTTAATTC
ACCAGTAAGAGCATTTAAAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MEQQLTFKRHRRLRASTSMRSLVRETHLRPTDFIYPIFVIEGSNIKNEIPSMPGIYQLSFDHLLEEVQEAYDLGIRSIIL
FGVVSNKDEIGSEAFHDHGIIQEATRMVKKDFPDMLVVADTCLCEYTSHGHCGVIQNRDVDNDASLELLAKTAVSQAKAG
ADIIAPSNMMDGFVVVIRQALDEAGFNHIPIMSYAVKYASAFYGPFRDAADSTPQFGDRKTYQMDPANRLEAIREATSDV
EEGADFLIVKPALSYLDIVREVRDNFDLPVVAYNVSGEYSMVKAAAQNGWIDERALVMEKLTSMKRAGSDLIISYFAKDV
AKWLQESN

Sequences:

>Translated_328_residues
MEQQLTFKRHRRLRASTSMRSLVRETHLRPTDFIYPIFVIEGSNIKNEIPSMPGIYQLSFDHLLEEVQEAYDLGIRSIIL
FGVVSNKDEIGSEAFHDHGIIQEATRMVKKDFPDMLVVADTCLCEYTSHGHCGVIQNRDVDNDASLELLAKTAVSQAKAG
ADIIAPSNMMDGFVVVIRQALDEAGFNHIPIMSYAVKYASAFYGPFRDAADSTPQFGDRKTYQMDPANRLEAIREATSDV
EEGADFLIVKPALSYLDIVREVRDNFDLPVVAYNVSGEYSMVKAAAQNGWIDERALVMEKLTSMKRAGSDLIISYFAKDV
AKWLQESN
>Mature_328_residues
MEQQLTFKRHRRLRASTSMRSLVRETHLRPTDFIYPIFVIEGSNIKNEIPSMPGIYQLSFDHLLEEVQEAYDLGIRSIIL
FGVVSNKDEIGSEAFHDHGIIQEATRMVKKDFPDMLVVADTCLCEYTSHGHCGVIQNRDVDNDASLELLAKTAVSQAKAG
ADIIAPSNMMDGFVVVIRQALDEAGFNHIPIMSYAVKYASAFYGPFRDAADSTPQFGDRKTYQMDPANRLEAIREATSDV
EEGADFLIVKPALSYLDIVREVRDNFDLPVVAYNVSGEYSMVKAAAQNGWIDERALVMEKLTSMKRAGSDLIISYFAKDV
AKWLQESN

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=306, Percent_Identity=45.7516339869281, Blast_Score=280, Evalue=1e-75,
Organism=Escherichia coli, GI87081728, Length=316, Percent_Identity=53.1645569620253, Blast_Score=330, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6321398, Length=325, Percent_Identity=40.6153846153846, Blast_Score=245, Evalue=6e-66,
Organism=Drosophila melanogaster, GI21358291, Length=320, Percent_Identity=39.375, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36790; Mature: 36790

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQQLTFKRHRRLRASTSMRSLVRETHLRPTDFIYPIFVIEGSNIKNEIPSMPGIYQLSF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHCCCCCCEEEECH
DHLLEEVQEAYDLGIRSIILFGVVSNKDEIGSEAFHDHGIIQEATRMVKKDFPDMLVVAD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
TCLCEYTSHGHCGVIQNRDVDNDASLELLAKTAVSQAKAGADIIAPSNMMDGFVVVIRQA
HHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
LDEAGFNHIPIMSYAVKYASAFYGPFRDAADSTPQFGDRKTYQMDPANRLEAIREATSDV
HHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHH
EEGADFLIVKPALSYLDIVREVRDNFDLPVVAYNVSGEYSMVKAAAQNGWIDERALVMEK
HCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHH
LTSMKRAGSDLIISYFAKDVAKWLQESN
HHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQQLTFKRHRRLRASTSMRSLVRETHLRPTDFIYPIFVIEGSNIKNEIPSMPGIYQLSF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHCCCCCCEEEECH
DHLLEEVQEAYDLGIRSIILFGVVSNKDEIGSEAFHDHGIIQEATRMVKKDFPDMLVVAD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
TCLCEYTSHGHCGVIQNRDVDNDASLELLAKTAVSQAKAGADIIAPSNMMDGFVVVIRQA
HHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
LDEAGFNHIPIMSYAVKYASAFYGPFRDAADSTPQFGDRKTYQMDPANRLEAIREATSDV
HHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHH
EEGADFLIVKPALSYLDIVREVRDNFDLPVVAYNVSGEYSMVKAAAQNGWIDERALVMEK
HCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHH
LTSMKRAGSDLIISYFAKDVAKWLQESN
HHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]