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The map label for this gene is hemL

Identifier: 23099520

GI number: 23099520

Start: 2092188

End: 2093474

Strand: Reverse

Name: hemL

Synonym: OB2065

Alternate gene names: 23099520

Gene position: 2093474-2092188 (Counterclockwise)

Preceding gene: 23099521

Following gene: 23099519

Centisome position: 57.66

GC content: 36.67

Gene sequence:

>1287_bases
ATGACACTTAATCAGTCAATTGATGCGTATAAAGAAGCTGTTGATTTAATGCCGGGTGGGGTTAATTCACCAGTAAGAGC
ATTTAAATCTGTCGGGATGAATCCGATTTTTATGAAAGAAGGAAAGGGTTCTAAAATAGTAGATATTGATAACAATGAAT
ATATTGATTATGTCTTAAGTTGGGGTCCATTAATTCTTGGTCATTCTGATGAACGTGTAGTAAAAAAACTTCAAGATATT
ACTGCTAAAGGTACAAGTTTTGGCGCACCAACACTATTAGAAAATGATTTAGCTAAACTTGTAATTGATCGTGTTCCTTC
AATTGAAATGGTTCGAATGGTTAACTCAGGTACAGAAGCAACGATGAGTGCTATTCGATTAGCTAGAGGCTACACGGATA
GAGATTTAATCCTAAAGTTTGAAGGAAGTTATCATGGTCATGGAGACTCTCTGCTTATCAAAGCGGGATCAGGCGTGGCA
ACTCTTGGATTACCAGATAGTCCTGGAGTACCTGAAAATATTGCAAAGAATACCATTACTGTACCTTATAATGATACAGA
CAGTTTGAAATTAGCTTTTGAAAATTATGGAGACCGCATAGCAGCGATAATTATGGAACCAGTATGTGGAAATATGGGAG
TAGTTCCACCGAAAGATGGTTTCTTACAATATGTTCGACATATTACGGAGGAAAATGGTTCCTTATTAATATTTGATGAA
GTAATGACTGGATTTAGAGTCGGTTATCATTGTGCACAAGGTCATTATGATGTAACTCCTGATTTAACATGCCTAGGAAA
AGTAATCGGTGGTGGATTACCGGTTGGTGCTTATGGTGGTAAGCGCGAAATCATGGAACAGATAGCTCCAACAGGTCCTA
TATATCAAGCTGGTACACTATCTGGTAACCCATTAGCTATGACTGCAGGATATGAGACATTATCTTCTTTATCAGAAGAA
TCCTATGAAGATATCAATAATAAAGTGGATACGTTAGTGAATGGGTTTAGAGATGCTGCCGACAAATTTGATATTCCGAT
TCATATTAATCGAGCAGGTTCCATGGTTGGAGTATTTTTCACAGATGAAGAGGTAATTAACTTTGAGACAGCTCAAACCT
CAAATCTTGATTACTTTGCTCAGTATTATCGCTCGATGGTTAATGAAGGGATATTCTTGCCACCTTCTCAATTTGAAGGA
TTATTCCTTTCCACTGCTCACACTGATGACGATATAGAAAAAACCGTTAAAGCTATTCATAAAGCTTTTGAACAAATAGA
AAAATAA

Upstream 100 bases:

>100_bases
ACTAACATCGATGAAACGAGCAGGATCTGATTTGATTATTTCTTATTTTGCTAAAGATGTTGCGAAATGGTTACAGGAAT
CGAACTAAGGAGGTATTTTA

Downstream 100 bases:

>100_bases
AATTCCATATTTATTTACAAAGCAATGTATGATGAGGGGATTCCTTCATTACACATTGCTTTTTTTGCATATCCTTGTTG
GTAACAACATATAAATGTTA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTLNQSIDAYKEAVDLMPGGVNSPVRAFKSVGMNPIFMKEGKGSKIVDIDNNEYIDYVLSWGPLILGHSDERVVKKLQDI
TAKGTSFGAPTLLENDLAKLVIDRVPSIEMVRMVNSGTEATMSAIRLARGYTDRDLILKFEGSYHGHGDSLLIKAGSGVA
TLGLPDSPGVPENIAKNTITVPYNDTDSLKLAFENYGDRIAAIIMEPVCGNMGVVPPKDGFLQYVRHITEENGSLLIFDE
VMTGFRVGYHCAQGHYDVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPTGPIYQAGTLSGNPLAMTAGYETLSSLSEE
SYEDINNKVDTLVNGFRDAADKFDIPIHINRAGSMVGVFFTDEEVINFETAQTSNLDYFAQYYRSMVNEGIFLPPSQFEG
LFLSTAHTDDDIEKTVKAIHKAFEQIEK

Sequences:

>Translated_428_residues
MTLNQSIDAYKEAVDLMPGGVNSPVRAFKSVGMNPIFMKEGKGSKIVDIDNNEYIDYVLSWGPLILGHSDERVVKKLQDI
TAKGTSFGAPTLLENDLAKLVIDRVPSIEMVRMVNSGTEATMSAIRLARGYTDRDLILKFEGSYHGHGDSLLIKAGSGVA
TLGLPDSPGVPENIAKNTITVPYNDTDSLKLAFENYGDRIAAIIMEPVCGNMGVVPPKDGFLQYVRHITEENGSLLIFDE
VMTGFRVGYHCAQGHYDVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPTGPIYQAGTLSGNPLAMTAGYETLSSLSEE
SYEDINNKVDTLVNGFRDAADKFDIPIHINRAGSMVGVFFTDEEVINFETAQTSNLDYFAQYYRSMVNEGIFLPPSQFEG
LFLSTAHTDDDIEKTVKAIHKAFEQIEK
>Mature_427_residues
TLNQSIDAYKEAVDLMPGGVNSPVRAFKSVGMNPIFMKEGKGSKIVDIDNNEYIDYVLSWGPLILGHSDERVVKKLQDIT
AKGTSFGAPTLLENDLAKLVIDRVPSIEMVRMVNSGTEATMSAIRLARGYTDRDLILKFEGSYHGHGDSLLIKAGSGVAT
LGLPDSPGVPENIAKNTITVPYNDTDSLKLAFENYGDRIAAIIMEPVCGNMGVVPPKDGFLQYVRHITEENGSLLIFDEV
MTGFRVGYHCAQGHYDVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPTGPIYQAGTLSGNPLAMTAGYETLSSLSEES
YEDINNKVDTLVNGFRDAADKFDIPIHINRAGSMVGVFFTDEEVINFETAQTSNLDYFAQYYRSMVNEGIFLPPSQFEGL
FLSTAHTDDDIEKTVKAIHKAFEQIEK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI13994255, Length=370, Percent_Identity=26.2162162162162, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI4557809, Length=313, Percent_Identity=29.073482428115, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI24119277, Length=321, Percent_Identity=27.4143302180685, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI284507298, Length=232, Percent_Identity=31.4655172413793, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=51.1737089201878, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI145693181, Length=338, Percent_Identity=31.3609467455621, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789016, Length=353, Percent_Identity=28.8951841359773, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788044, Length=320, Percent_Identity=31.5625, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789759, Length=332, Percent_Identity=28.9156626506024, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1787560, Length=355, Percent_Identity=29.0140845070423, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1786991, Length=391, Percent_Identity=26.0869565217391, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25144271, Length=303, Percent_Identity=28.3828382838284, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=361, Percent_Identity=26.0387811634349, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32564660, Length=438, Percent_Identity=25.1141552511416, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=308, Percent_Identity=27.9220779220779, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=351, Percent_Identity=25.3561253561254, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI161085790, Length=432, Percent_Identity=24.0740740740741, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21357415, Length=301, Percent_Identity=26.578073089701, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356575, Length=383, Percent_Identity=26.3707571801567, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28574759, Length=397, Percent_Identity=24.4332493702771, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA2_OCEIH (Q8CZE3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692986.1
- ProteinModelPortal:   Q8CZE3
- SMR:   Q8CZE3
- GeneID:   1015987
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2065
- NMPDR:   fig|221109.1.peg.2063
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   OIHE221109:OB2065-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46691; Mature: 46560

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNQSIDAYKEAVDLMPGGVNSPVRAFKSVGMNPIFMKEGKGSKIVDIDNNEYIDYVLS
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCEEEEEEE
WGPLILGHSDERVVKKLQDITAKGTSFGAPTLLENDLAKLVIDRVPSIEMVRMVNSGTEA
CCCEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHH
TMSAIRLARGYTDRDLILKFEGSYHGHGDSLLIKAGSGVATLGLPDSPGVPENIAKNTIT
HHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCCHHHCCCEEE
VPYNDTDSLKLAFENYGDRIAAIIMEPVCGNMGVVPPKDGFLQYVRHITEENGSLLIFDE
ECCCCCCCEEEEEHHCCCCEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHH
VMTGFRVGYHCAQGHYDVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPTGPIYQAGTL
HHHHHHHHHEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPLAMTAGYETLSSLSEESYEDINNKVDTLVNGFRDAADKFDIPIHINRAGSMVGVFF
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEE
TDEEVINFETAQTSNLDYFAQYYRSMVNEGIFLPPSQFEGLFLSTAHTDDDIEKTVKAIH
ECCCEEEEECCCCCCHHHHHHHHHHHHHCCEECCCCCCCCEEEEECCCCHHHHHHHHHHH
KAFEQIEK
HHHHHHCC
>Mature Secondary Structure 
TLNQSIDAYKEAVDLMPGGVNSPVRAFKSVGMNPIFMKEGKGSKIVDIDNNEYIDYVLS
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCEEEEEEE
WGPLILGHSDERVVKKLQDITAKGTSFGAPTLLENDLAKLVIDRVPSIEMVRMVNSGTEA
CCCEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHH
TMSAIRLARGYTDRDLILKFEGSYHGHGDSLLIKAGSGVATLGLPDSPGVPENIAKNTIT
HHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCCHHHCCCEEE
VPYNDTDSLKLAFENYGDRIAAIIMEPVCGNMGVVPPKDGFLQYVRHITEENGSLLIFDE
ECCCCCCCEEEEEHHCCCCEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHH
VMTGFRVGYHCAQGHYDVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPTGPIYQAGTL
HHHHHHHHHEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPLAMTAGYETLSSLSEESYEDINNKVDTLVNGFRDAADKFDIPIHINRAGSMVGVFF
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEE
TDEEVINFETAQTSNLDYFAQYYRSMVNEGIFLPPSQFEGLFLSTAHTDDDIEKTVKAIH
ECCCEEEEECCCCCCHHHHHHHHHHHHHCCEECCCCCCCCEEEEECCCCHHHHHHHHHHH
KAFEQIEK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376