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The map label for this gene is pdhC [H]

Identifier: 23098869

GI number: 23098869

Start: 1457309

End: 1458592

Strand: Direct

Name: pdhC [H]

Synonym: OB1414

Alternate gene names: 23098869

Gene position: 1457309-1458592 (Clockwise)

Preceding gene: 23098868

Following gene: 23098870

Centisome position: 40.14

GC content: 36.37

Gene sequence:

>1284_bases
ATGGCATTTAATTTTAAGTTACCGGATATCGGTGAAGGTATACATGAAGGTGAAATAGTAAAATGGTTCGTAAAAGAAGG
CGACGAAGTAAAAGAAGATGATGTACTTTGTGAAGTACAAAATGATAAATCCGTTGTAGAAATTCCTTCTCAAGTAGATG
GAAAAGTTACAAAGATTCACGTTGCTGAAGGTGACGTTGCAGTTGTAGGAGATACACTAATTTCTTTTGAAGCAGAAGGT
TATGATGATGAAGAAGGCGACAGTGCTGATGATTCATCTTCAGATTCAAAAGAAGAGAAATCAACGGATTCGAAAGAAGA
AGAAAAAGAAGCTTCTAAAGAAGAGTCAAGTGAGCAATCTGATGATACTCGAGTGATTGCAATGCCTTCTGTACGCAAAT
TTGCACGTGATAATGATGTTAATATTAAAGATGTTAACGGAACTGGTAAAAATGGACGTATTCTTAAAGAAGATGTAGAA
AGCTACCTAAGCGGTGATCAGCCATCTAGTGAAGTAGCCGAGGACAAAGCTGAAGCTTCTTCTGAAGATAAGCAAGAAAC
GAAAGCTGCACCTCAAGGTCAATATCCAGAGACTCGTGAGAAGATGACTGCAATCCGTAAATCTATTGCTAAATCAATGG
TTAACTCGAAATCAAAAGCACCACATGTTACACTAATGGACGAAATTGATGTAACTGAATTAGTAGCACATCGTAAGAAG
TTTAAAGCAGTTGCTGCTGAGCAAGATATTAAATTAACTTATTTACCATATGTAGTTAAAGCATTAGTATCTGCGTCTAA
GAAATTCCCTATTCTAAATTCTTATATTGATGAGAATACAGATGAAATTGTTGAGAAACATTATTACAACATTGGTATTG
CAGCAGACACAGATAGAGGTCTACTAGTTCCAGTTGTAAAAGATTCTGATAAGAAATCTATTTTCCAAATTTCTCAAGAA
ATCAATGAATTGGCAGGGAAAGCTCGAGATGGTAAACTTAAACCTGATGAGATGAAAGGTGCTTCTAACACTATTTCTAA
TATTGGTTCTGCTGGTGGACAGTGGTTTACTCCAGTATTAAATTATCCAGAAGCAGTAATTCTAGGTATCGGACGTATTG
CTGATAAACCAGTTGTTCGCGATGGAGAAATTGTTGTAGCTCCAGTTCTCTCTGTATCTCTAAGCTTTGATCATCGTATC
GTTGACGGCGCAACAGCACAACTTGCACTAAATCAAATTAAACGATTATTGAATGATCCACAATTAATTATGATGGAGGC
GTAA

Upstream 100 bases:

>100_bases
TCTCTGATGCAGAGGAAACATGGTTGCCTAACCATAATGATATCGTAGAGAAAGTTAATGAAGTAATTAATTTCTAAAAT
AGTTTAGGAGGTTTCATAGC

Downstream 100 bases:

>100_bases
GTAATGGTAGTAGGAGATTTTCCGGTAGAAGTAGACACTCTAGTCGTTGGGGCAGGACCTGGTGGCTATGTTGCAGCGAT
TCGTGCTGCACAATTAGGTC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAFNFKLPDIGEGIHEGEIVKWFVKEGDEVKEDDVLCEVQNDKSVVEIPSQVDGKVTKIHVAEGDVAVVGDTLISFEAEG
YDDEEGDSADDSSSDSKEEKSTDSKEEEKEASKEESSEQSDDTRVIAMPSVRKFARDNDVNIKDVNGTGKNGRILKEDVE
SYLSGDQPSSEVAEDKAEASSEDKQETKAAPQGQYPETREKMTAIRKSIAKSMVNSKSKAPHVTLMDEIDVTELVAHRKK
FKAVAAEQDIKLTYLPYVVKALVSASKKFPILNSYIDENTDEIVEKHYYNIGIAADTDRGLLVPVVKDSDKKSIFQISQE
INELAGKARDGKLKPDEMKGASNTISNIGSAGGQWFTPVLNYPEAVILGIGRIADKPVVRDGEIVVAPVLSVSLSFDHRI
VDGATAQLALNQIKRLLNDPQLIMMEA

Sequences:

>Translated_427_residues
MAFNFKLPDIGEGIHEGEIVKWFVKEGDEVKEDDVLCEVQNDKSVVEIPSQVDGKVTKIHVAEGDVAVVGDTLISFEAEG
YDDEEGDSADDSSSDSKEEKSTDSKEEEKEASKEESSEQSDDTRVIAMPSVRKFARDNDVNIKDVNGTGKNGRILKEDVE
SYLSGDQPSSEVAEDKAEASSEDKQETKAAPQGQYPETREKMTAIRKSIAKSMVNSKSKAPHVTLMDEIDVTELVAHRKK
FKAVAAEQDIKLTYLPYVVKALVSASKKFPILNSYIDENTDEIVEKHYYNIGIAADTDRGLLVPVVKDSDKKSIFQISQE
INELAGKARDGKLKPDEMKGASNTISNIGSAGGQWFTPVLNYPEAVILGIGRIADKPVVRDGEIVVAPVLSVSLSFDHRI
VDGATAQLALNQIKRLLNDPQLIMMEA
>Mature_426_residues
AFNFKLPDIGEGIHEGEIVKWFVKEGDEVKEDDVLCEVQNDKSVVEIPSQVDGKVTKIHVAEGDVAVVGDTLISFEAEGY
DDEEGDSADDSSSDSKEEKSTDSKEEEKEASKEESSEQSDDTRVIAMPSVRKFARDNDVNIKDVNGTGKNGRILKEDVES
YLSGDQPSSEVAEDKAEASSEDKQETKAAPQGQYPETREKMTAIRKSIAKSMVNSKSKAPHVTLMDEIDVTELVAHRKKF
KAVAAEQDIKLTYLPYVVKALVSASKKFPILNSYIDENTDEIVEKHYYNIGIAADTDRGLLVPVVKDSDKKSIFQISQEI
NELAGKARDGKLKPDEMKGASNTISNIGSAGGQWFTPVLNYPEAVILGIGRIADKPVVRDGEIVVAPVLSVSLSFDHRIV
DGATAQLALNQIKRLLNDPQLIMMEA

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=36.5740740740741, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI31711992, Length=436, Percent_Identity=29.1284403669725, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI19923748, Length=239, Percent_Identity=34.7280334728033, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=28.1879194630872, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI203098816, Length=447, Percent_Identity=28.1879194630872, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI260898739, Length=140, Percent_Identity=37.8571428571429, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786305, Length=422, Percent_Identity=34.1232227488152, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1786946, Length=421, Percent_Identity=31.353919239905, Blast_Score=219, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17537937, Length=429, Percent_Identity=34.7319347319347, Blast_Score=254, Evalue=9e-68,
Organism=Caenorhabditis elegans, GI25146366, Length=420, Percent_Identity=31.1904761904762, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=30.3571428571429, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=29.5454545454545, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=29.7423887587822, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6324258, Length=445, Percent_Identity=27.4157303370787, Blast_Score=166, Evalue=5e-42,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=37.9391100702576, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=32.1888412017167, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=33.1877729257642, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=33.1877729257642, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46808; Mature: 46676

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNFKLPDIGEGIHEGEIVKWFVKEGDEVKEDDVLCEVQNDKSVVEIPSQVDGKVTKIH
CCCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEE
VAEGDVAVVGDTLISFEAEGYDDEEGDSADDSSSDSKEEKSTDSKEEEKEASKEESSEQS
EECCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCC
DDTRVIAMPSVRKFARDNDVNIKDVNGTGKNGRILKEDVESYLSGDQPSSEVAEDKAEAS
CCCEEEECCHHHHHHCCCCCCEEECCCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHCC
SEDKQETKAAPQGQYPETREKMTAIRKSIAKSMVNSKSKAPHVTLMDEIDVTELVAHRKK
CHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHH
FKAVAAEQDIKLTYLPYVVKALVSASKKFPILNSYIDENTDEIVEKHYYNIGIAADTDRG
HHHHHCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHEEEEEECCCCC
LLVPVVKDSDKKSIFQISQEINELAGKARDGKLKPDEMKGASNTISNIGSAGGQWFTPVL
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHCCCCCEEHHHH
NYPEAVILGIGRIADKPVVRDGEIVVAPVLSVSLSFDHRIVDGATAQLALNQIKRLLNDP
CCCCEEEEECHHHCCCCCCCCCCEEEEEEEEEEECCCCEEECCHHHHHHHHHHHHHHCCC
QLIMMEA
CEEEEEC
>Mature Secondary Structure 
AFNFKLPDIGEGIHEGEIVKWFVKEGDEVKEDDVLCEVQNDKSVVEIPSQVDGKVTKIH
CCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEE
VAEGDVAVVGDTLISFEAEGYDDEEGDSADDSSSDSKEEKSTDSKEEEKEASKEESSEQS
EECCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCC
DDTRVIAMPSVRKFARDNDVNIKDVNGTGKNGRILKEDVESYLSGDQPSSEVAEDKAEAS
CCCEEEECCHHHHHHCCCCCCEEECCCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHCC
SEDKQETKAAPQGQYPETREKMTAIRKSIAKSMVNSKSKAPHVTLMDEIDVTELVAHRKK
CHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHH
FKAVAAEQDIKLTYLPYVVKALVSASKKFPILNSYIDENTDEIVEKHYYNIGIAADTDRG
HHHHHCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHEEEEEECCCCC
LLVPVVKDSDKKSIFQISQEINELAGKARDGKLKPDEMKGASNTISNIGSAGGQWFTPVL
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHCCCCCEEHHHH
NYPEAVILGIGRIADKPVVRDGEIVVAPVLSVSLSFDHRIVDGATAQLALNQIKRLLNDP
CCCCEEEEECHHHCCCCCCCCCCEEEEEEEEEEECCCCEEECCHHHHHHHHHHHHHHCCC
QLIMMEA
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]