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The map label for this gene is pdhD [H]

Identifier: 23098870

GI number: 23098870

Start: 1458596

End: 1460002

Strand: Direct

Name: pdhD [H]

Synonym: OB1415

Alternate gene names: 23098870

Gene position: 1458596-1460002 (Clockwise)

Preceding gene: 23098869

Following gene: 23098871

Centisome position: 40.18

GC content: 37.95

Gene sequence:

>1407_bases
ATGGTAGTAGGAGATTTTCCGGTAGAAGTAGACACTCTAGTCGTTGGGGCAGGACCTGGTGGCTATGTTGCAGCGATTCG
TGCTGCACAATTAGGTCAAAAAGTAACAATCGTAGATAAAGGAGCTTTAGGTGGAGTTTGCTTAAATGTTGGTTGTATTC
CTTCTAAAGCACTTATTGAAGCTGGCCATAAATATGAAAATGCGCATGGATCTGAAGATCTTGGAATTAAAACAGATAAA
GTAGAAGTAGACTTTTCTAAAGTTCAAGACTGGAAAGGTTCTGTTGTTAACAAACTTACTTCAGGTGTTGAGAGCTTATT
AAAAGGTAACAAAGTAGATATCGTAAAAGGTGAAGCATATTTCGTTGATGCAAATACTGCTAAAATCGCTGGAGAGAGCA
GCTCTCAAACGTATAAATTTAAAGATTGTATCATTGCAACAGGTTCTTCACCAATTGAAATTCCGTCATTTAAGTTCTCT
GATCGCGTGTTAGATTCTACAGGCGCATTGAGCTTAAAAGAAATTCCGAAGAAAATGGTAGTAATTGGTTCTGGATATGT
AGGTACAGAACTTGGTACTGCATATGCTAACTTTGGAACTGAAATTACTTTCTTAGAAGGTGCGAAAGACATCTTAGGTG
GATTTGAGAAACAAATGACGCAACTTGTTAAGAAAGGCCTTAAGAAAAAAGGTGCTACAATTATCACAGAAGCAATGGCT
AAAGGCGTTGAAGAAACAAAAGACGGTGTAAAAGTTTCTTATGAAGTAAATGGTAAAGAAGAAACTATTGAAGCAGATTA
TGTACTTGTAACAGTAGGTCGTCGTCCGAACACTGATGAGCTTGGTTTAGAGCAAATGGGTGTTAAGATGACAGATAGAG
GCCATATTGAAATAGATAAACAATGTAAAACAAGTGTTGATAATATCTATGCAATTGGTGATATCGTTGAAGGACCTGCA
CTTGCACACAAGGCTTCTTATGAAGGTAAGATTGCTGCTGAAGCTATTAGTGGTGAAAAATCTGAAATTGACTATGTAGG
AATACCTGCAGTGGCATTTACAGAACCTGAACTAGCAACAGTAGGTTTATCTGAACAAGACGCGAAAGATGCTGGTTATG
ATGTGAAGGTTGGTAAATTCCCATTCGGAGCAAACGGCCGTGCACTTTCTTTAAATAACACAGAAGGTTTCTTAAAACTA
ATCACTCGTAAAGATGATGGATTAGTTATCGGTGGTCAAATTGCTGGTCCAAATGCTAGTGATATGATTTCTGAAATTGG
TTTAGCAATTGAAGCAGGCATGACTGCTGAAGATATTTCTCTAACTATCCATGCTCATCCAACACTAGGTGAGATTACAA
TGGAAGCAGCGGAAGTCGCTTTAGGAACACCAATTCATACTATGTAA

Upstream 100 bases:

>100_bases
TGATCATCGTATCGTTGACGGCGCAACAGCACAACTTGCACTAAATCAAATTAAACGATTATTGAATGATCCACAATTAA
TTATGATGGAGGCGTAAGTA

Downstream 100 bases:

>100_bases
TATAGGAAAAACTGCCAAGTAATTTTTACTTGGCAGTTTTTTGCTTATTCTATAGTAATGTTTCAATCTCTTGAATAATC
TCGGCTCTACAATGAATGTG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MVVGDFPVEVDTLVVGAGPGGYVAAIRAAQLGQKVTIVDKGALGGVCLNVGCIPSKALIEAGHKYENAHGSEDLGIKTDK
VEVDFSKVQDWKGSVVNKLTSGVESLLKGNKVDIVKGEAYFVDANTAKIAGESSSQTYKFKDCIIATGSSPIEIPSFKFS
DRVLDSTGALSLKEIPKKMVVIGSGYVGTELGTAYANFGTEITFLEGAKDILGGFEKQMTQLVKKGLKKKGATIITEAMA
KGVEETKDGVKVSYEVNGKEETIEADYVLVTVGRRPNTDELGLEQMGVKMTDRGHIEIDKQCKTSVDNIYAIGDIVEGPA
LAHKASYEGKIAAEAISGEKSEIDYVGIPAVAFTEPELATVGLSEQDAKDAGYDVKVGKFPFGANGRALSLNNTEGFLKL
ITRKDDGLVIGGQIAGPNASDMISEIGLAIEAGMTAEDISLTIHAHPTLGEITMEAAEVALGTPIHTM

Sequences:

>Translated_468_residues
MVVGDFPVEVDTLVVGAGPGGYVAAIRAAQLGQKVTIVDKGALGGVCLNVGCIPSKALIEAGHKYENAHGSEDLGIKTDK
VEVDFSKVQDWKGSVVNKLTSGVESLLKGNKVDIVKGEAYFVDANTAKIAGESSSQTYKFKDCIIATGSSPIEIPSFKFS
DRVLDSTGALSLKEIPKKMVVIGSGYVGTELGTAYANFGTEITFLEGAKDILGGFEKQMTQLVKKGLKKKGATIITEAMA
KGVEETKDGVKVSYEVNGKEETIEADYVLVTVGRRPNTDELGLEQMGVKMTDRGHIEIDKQCKTSVDNIYAIGDIVEGPA
LAHKASYEGKIAAEAISGEKSEIDYVGIPAVAFTEPELATVGLSEQDAKDAGYDVKVGKFPFGANGRALSLNNTEGFLKL
ITRKDDGLVIGGQIAGPNASDMISEIGLAIEAGMTAEDISLTIHAHPTLGEITMEAAEVALGTPIHTM
>Mature_468_residues
MVVGDFPVEVDTLVVGAGPGGYVAAIRAAQLGQKVTIVDKGALGGVCLNVGCIPSKALIEAGHKYENAHGSEDLGIKTDK
VEVDFSKVQDWKGSVVNKLTSGVESLLKGNKVDIVKGEAYFVDANTAKIAGESSSQTYKFKDCIIATGSSPIEIPSFKFS
DRVLDSTGALSLKEIPKKMVVIGSGYVGTELGTAYANFGTEITFLEGAKDILGGFEKQMTQLVKKGLKKKGATIITEAMA
KGVEETKDGVKVSYEVNGKEETIEADYVLVTVGRRPNTDELGLEQMGVKMTDRGHIEIDKQCKTSVDNIYAIGDIVEGPA
LAHKASYEGKIAAEAISGEKSEIDYVGIPAVAFTEPELATVGLSEQDAKDAGYDVKVGKFPFGANGRALSLNNTEGFLKL
ITRKDDGLVIGGQIAGPNASDMISEIGLAIEAGMTAEDISLTIHAHPTLGEITMEAAEVALGTPIHTM

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=42.5162689804772, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI50301238, Length=456, Percent_Identity=28.5087719298246, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=30.3687635574837, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI291045266, Length=457, Percent_Identity=29.5404814004376, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI148277065, Length=451, Percent_Identity=28.6031042128603, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI33519430, Length=451, Percent_Identity=28.6031042128603, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI33519428, Length=451, Percent_Identity=28.6031042128603, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI33519426, Length=451, Percent_Identity=28.6031042128603, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI148277071, Length=451, Percent_Identity=28.6031042128603, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI291045268, Length=449, Percent_Identity=27.8396436525612, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1786307, Length=461, Percent_Identity=43.8177874186551, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1789915, Length=438, Percent_Identity=29.6803652968037, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI87081717, Length=446, Percent_Identity=27.5784753363229, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI87082354, Length=457, Percent_Identity=27.3522975929978, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789065, Length=207, Percent_Identity=21.256038647343, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=41.5754923413567, Blast_Score=338, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=29.2887029288703, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71983419, Length=455, Percent_Identity=28.7912087912088, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71983429, Length=455, Percent_Identity=28.7912087912088, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71982272, Length=472, Percent_Identity=26.6949152542373, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321091, Length=471, Percent_Identity=39.0658174097665, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=26.8343815513627, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=28.2937365010799, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21358499, Length=472, Percent_Identity=44.2796610169491, Blast_Score=351, Evalue=6e-97,
Organism=Drosophila melanogaster, GI24640549, Length=466, Percent_Identity=29.8283261802575, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24640553, Length=466, Percent_Identity=29.8283261802575, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24640551, Length=466, Percent_Identity=29.8283261802575, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17737741, Length=471, Percent_Identity=26.7515923566879, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49255; Mature: 49255

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFPVEVDTLVVGAGPGGYVAAIRAAQLGQKVTIVDKGALGGVCLNVGCIPSKALIE
CEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHH
AGHKYENAHGSEDLGIKTDKVEVDFSKVQDWKGSVVNKLTSGVESLLKGNKVDIVKGEAY
CCCCCCCCCCCCCCCEEECEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEE
FVDANTAKIAGESSSQTYKFKDCIIATGSSPIEIPSFKFSDRVLDSTGALSLKEIPKKMV
EEECCCEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCEEHHHCCCEEE
VIGSGYVGTELGTAYANFGTEITFLEGAKDILGGFEKQMTQLVKKGLKKKGATIITEAMA
EEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KGVEETKDGVKVSYEVNGKEETIEADYVLVTVGRRPNTDELGLEQMGVKMTDRGHIEIDK
CCHHHCCCCEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCHHHHCCEECCCCCEEECH
QCKTSVDNIYAIGDIVEGPALAHKASYEGKIAAEAISGEKSEIDYVGIPAVAFTEPELAT
HHHCCCCCEEEECCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCEECCCEEEECCCCEEE
VGLSEQDAKDAGYDVKVGKFPFGANGRALSLNNTEGFLKLITRKDDGLVIGGQIAGPNAS
ECCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEECCCCEEEECEECCCCHH
DMISEIGLAIEAGMTAEDISLTIHAHPTLGEITMEAAEVALGTPIHTM
HHHHHHCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVVGDFPVEVDTLVVGAGPGGYVAAIRAAQLGQKVTIVDKGALGGVCLNVGCIPSKALIE
CEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHH
AGHKYENAHGSEDLGIKTDKVEVDFSKVQDWKGSVVNKLTSGVESLLKGNKVDIVKGEAY
CCCCCCCCCCCCCCCEEECEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEE
FVDANTAKIAGESSSQTYKFKDCIIATGSSPIEIPSFKFSDRVLDSTGALSLKEIPKKMV
EEECCCEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHCCCCCEEHHHCCCEEE
VIGSGYVGTELGTAYANFGTEITFLEGAKDILGGFEKQMTQLVKKGLKKKGATIITEAMA
EEECCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KGVEETKDGVKVSYEVNGKEETIEADYVLVTVGRRPNTDELGLEQMGVKMTDRGHIEIDK
CCHHHCCCCEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCHHHHCCEECCCCCEEECH
QCKTSVDNIYAIGDIVEGPALAHKASYEGKIAAEAISGEKSEIDYVGIPAVAFTEPELAT
HHHCCCCCEEEECCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCEECCCEEEECCCCEEE
VGLSEQDAKDAGYDVKVGKFPFGANGRALSLNNTEGFLKLITRKDDGLVIGGQIAGPNAS
ECCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEECCCCEEEECEECCCCHH
DMISEIGLAIEAGMTAEDISLTIHAHPTLGEITMEAAEVALGTPIHTM
HHHHHHCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]