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The map label for this gene is purF [H]

Identifier: 23098201

GI number: 23098201

Start: 793799

End: 795205

Strand: Direct

Name: purF [H]

Synonym: OB0746

Alternate gene names: 23098201

Gene position: 793799-795205 (Clockwise)

Preceding gene: 23098200

Following gene: 23098202

Centisome position: 21.86

GC content: 40.72

Gene sequence:

>1407_bases
ATGCTTGCTGAAATCAAAGGCATAAATGAAGAATGTGGTGTTTTTGGCATCTGGGGTCACGAAAAAGCAGCTGAATTAAC
TTATTATGGCTTACATTCCATGCAACATCGAGGACAAGAAGGTGCTGGGGTTGTTGTTAATACGGGTAGCGAACTAAAAG
GACATAAAGGTTTGGGTCTTGTAAATGATGTGTTCAAACGAGTTAAATTTGATGACTTGGAAGGAAGTGTAGCAGTAGGG
CACGTCCGGTATGCGACACAAGGTGGGAGAGGCATTGAAAATGTCCAACCTTTATTATTTCAGTCACAAACTGGCGGAAT
GGCGCTAGCACATAATGGTAATCTGATGAACGCACATGAACTGCGTGGCGAATTAGAAGGAGAAGGAAGCATATTACAAA
CCTCTTCGGATACGGAAGTCTTAGCTCATTTAATTAAACGTAGTGGAGAAGTAACAGAGGACTCTATAGCTCGTGCACTG
AATCGTGTAGTCGGTGCATACGCATATATCATACTGCAAGAAGATAAAATGTTTGCAGCACTTGATCCTTCAGGTATTCG
TCCACTTTCTATTGGTAGATTGGGAGATGCTTATGTGATAGCTTCCGAGACGTGTGCTTTTGACCAAATTGGTGCAACAT
TTGAACGAGAAGTGCTTCCAGGGGAATTGATCACAATTAGTGATGAAGGGATGACCTCTACTCGATTTGCACCAAGAGAC
AAGCGACGAATGTGTGCAATGGAATATGTCTACCTTTCTAGACCAGATAGTGATGTGAACTATGTGAATGTCCATGCGTC
GAGAAAACAAATGGGGAAAGAGTTAGCGAAAGAATCATCCGTTGATGCGGATATCGTTGTCGGTGTACCTGATTCTAGTA
TTTCTGCTGCAATCGGCTATGCGGAAGAAAGTGGACTCCCTTATGAAATGGGCATCATAAAAAATCGTTATGTCGGACGT
ACCTTTATCCAACCATCTCAAGAACTCAGAGAATTAGGGGTGAAGATGAAGCTTTCACCTGTTCGAAGCATTATTGAAGG
AAAACGAGTTGTTATGATTGATGATTCAATTGTACGTGGGACAACAAGCCGACGCATTGTTCAGATGTTACGAGAAGCGG
GTGCAAAAGAGATTCATGTTCGGATAGCTTCTCCAGCGATTCAAAATCCATGTTATTACGGAATTGATATGTCTACTAAA
AAAGAATTAATTGGAGCCAATTATTCTGTTGATGAAATCAATGGAATTATAGGCTCAGATACGCTAGCTTATTTATCAGA
AAAAGGGTTGGAAAAAGCAATCGTAAAAGACAAAACGATTAATCAAGGTGTTTGTATGGCTTGTATGACTGGCAAATACC
CAGTAAAACAAGACGGTGAATTGGAAATTCATTATACAAGTTGTTAA

Upstream 100 bases:

>100_bases
AGGTTACTGATCAAGGGCAACTAACTATTTCTATTAGCGATAAAACAATTATTCACGAACGTGTCGAAGAATTAGAGAAT
CTTTGGAAGGGAGCTATTCC

Downstream 100 bases:

>100_bases
AAGAAGTTTACGTGGAGGGATTACCATGTCAGAAGTCTATAAACAAGCAGGAGTAGATGTTGAAAAAGGGTATGAAGCTG
TAGAACGGCTAAAAAAACAT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MLAEIKGINEECGVFGIWGHEKAAELTYYGLHSMQHRGQEGAGVVVNTGSELKGHKGLGLVNDVFKRVKFDDLEGSVAVG
HVRYATQGGRGIENVQPLLFQSQTGGMALAHNGNLMNAHELRGELEGEGSILQTSSDTEVLAHLIKRSGEVTEDSIARAL
NRVVGAYAYIILQEDKMFAALDPSGIRPLSIGRLGDAYVIASETCAFDQIGATFEREVLPGELITISDEGMTSTRFAPRD
KRRMCAMEYVYLSRPDSDVNYVNVHASRKQMGKELAKESSVDADIVVGVPDSSISAAIGYAEESGLPYEMGIIKNRYVGR
TFIQPSQELRELGVKMKLSPVRSIIEGKRVVMIDDSIVRGTTSRRIVQMLREAGAKEIHVRIASPAIQNPCYYGIDMSTK
KELIGANYSVDEINGIIGSDTLAYLSEKGLEKAIVKDKTINQGVCMACMTGKYPVKQDGELEIHYTSC

Sequences:

>Translated_468_residues
MLAEIKGINEECGVFGIWGHEKAAELTYYGLHSMQHRGQEGAGVVVNTGSELKGHKGLGLVNDVFKRVKFDDLEGSVAVG
HVRYATQGGRGIENVQPLLFQSQTGGMALAHNGNLMNAHELRGELEGEGSILQTSSDTEVLAHLIKRSGEVTEDSIARAL
NRVVGAYAYIILQEDKMFAALDPSGIRPLSIGRLGDAYVIASETCAFDQIGATFEREVLPGELITISDEGMTSTRFAPRD
KRRMCAMEYVYLSRPDSDVNYVNVHASRKQMGKELAKESSVDADIVVGVPDSSISAAIGYAEESGLPYEMGIIKNRYVGR
TFIQPSQELRELGVKMKLSPVRSIIEGKRVVMIDDSIVRGTTSRRIVQMLREAGAKEIHVRIASPAIQNPCYYGIDMSTK
KELIGANYSVDEINGIIGSDTLAYLSEKGLEKAIVKDKTINQGVCMACMTGKYPVKQDGELEIHYTSC
>Mature_468_residues
MLAEIKGINEECGVFGIWGHEKAAELTYYGLHSMQHRGQEGAGVVVNTGSELKGHKGLGLVNDVFKRVKFDDLEGSVAVG
HVRYATQGGRGIENVQPLLFQSQTGGMALAHNGNLMNAHELRGELEGEGSILQTSSDTEVLAHLIKRSGEVTEDSIARAL
NRVVGAYAYIILQEDKMFAALDPSGIRPLSIGRLGDAYVIASETCAFDQIGATFEREVLPGELITISDEGMTSTRFAPRD
KRRMCAMEYVYLSRPDSDVNYVNVHASRKQMGKELAKESSVDADIVVGVPDSSISAAIGYAEESGLPYEMGIIKNRYVGR
TFIQPSQELRELGVKMKLSPVRSIIEGKRVVMIDDSIVRGTTSRRIVQMLREAGAKEIHVRIASPAIQNPCYYGIDMSTK
KELIGANYSVDEINGIIGSDTLAYLSEKGLEKAIVKDKTINQGVCMACMTGKYPVKQDGELEIHYTSC

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=508, Percent_Identity=40.5511811023622, Blast_Score=358, Evalue=7e-99,
Organism=Escherichia coli, GI1788651, Length=470, Percent_Identity=39.5744680851064, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1790167, Length=211, Percent_Identity=29.3838862559242, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=40, Blast_Score=345, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6323958, Length=459, Percent_Identity=35.7298474945534, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24659598, Length=486, Percent_Identity=40.9465020576132, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24659604, Length=457, Percent_Identity=41.5754923413567, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28573187, Length=492, Percent_Identity=41.0569105691057, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51077; Mature: 51077

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEIKGINEECGVFGIWGHEKAAELTYYGLHSMQHRGQEGAGVVVNTGSELKGHKGLGL
CCCCCCCCCCCCCEEEECCCCCHHHEEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCH
VNDVFKRVKFDDLEGSVAVGHVRYATQGGRGIENVQPLLFQSQTGGMALAHNGNLMNAHE
HHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEECCCCCEEEEECCCCCCHHH
LRGELEGEGSILQTSSDTEVLAHLIKRSGEVTEDSIARALNRVVGAYAYIILQEDKMFAA
HCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEECCCEEEE
LDPSGIRPLSIGRLGDAYVIASETCAFDQIGATFEREVLPGELITISDEGMTSTRFAPRD
ECCCCCCEEEECCCCCEEEEEECCCCHHHHCCCHHHCCCCCCEEEEECCCCCCCCCCCCH
KRRMCAMEYVYLSRPDSDVNYVNVHASRKQMGKELAKESSVDADIVVGVPDSSISAAIGY
HHHHHHEEEEEEECCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEECC
AEESGLPYEMGIIKNRYVGRTFIQPSQELRELGVKMKLSPVRSIIEGKRVVMIDDSIVRG
HHHCCCCEEECCCCCCCCCCHHCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHHHCC
TTSRRIVQMLREAGAKEIHVRIASPAIQNPCYYGIDMSTKKELIGANYSVDEINGIIGSD
CHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCHHHHHCCCCCHHHHCCCCCCH
TLAYLSEKGLEKAIVKDKTINQGVCMACMTGKYPVKQDGELEIHYTSC
HHHHHHHHCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MLAEIKGINEECGVFGIWGHEKAAELTYYGLHSMQHRGQEGAGVVVNTGSELKGHKGLGL
CCCCCCCCCCCCCEEEECCCCCHHHEEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCH
VNDVFKRVKFDDLEGSVAVGHVRYATQGGRGIENVQPLLFQSQTGGMALAHNGNLMNAHE
HHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEECCCCCEEEEECCCCCCHHH
LRGELEGEGSILQTSSDTEVLAHLIKRSGEVTEDSIARALNRVVGAYAYIILQEDKMFAA
HCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEEECCCEEEE
LDPSGIRPLSIGRLGDAYVIASETCAFDQIGATFEREVLPGELITISDEGMTSTRFAPRD
ECCCCCCEEEECCCCCEEEEEECCCCHHHHCCCHHHCCCCCCEEEEECCCCCCCCCCCCH
KRRMCAMEYVYLSRPDSDVNYVNVHASRKQMGKELAKESSVDADIVVGVPDSSISAAIGY
HHHHHHEEEEEEECCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEECC
AEESGLPYEMGIIKNRYVGRTFIQPSQELRELGVKMKLSPVRSIIEGKRVVMIDDSIVRG
HHHCCCCEEECCCCCCCCCCHHCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHHHCC
TTSRRIVQMLREAGAKEIHVRIASPAIQNPCYYGIDMSTKKELIGANYSVDEINGIIGSD
CHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCCHHHHHCCCCCHHHHCCCCCCH
TLAYLSEKGLEKAIVKDKTINQGVCMACMTGKYPVKQDGELEIHYTSC
HHHHHHHHCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]