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The map label for this gene is opuD [H]

Identifier: 23097925

GI number: 23097925

Start: 498579

End: 500129

Strand: Direct

Name: opuD [H]

Synonym: OB0470

Alternate gene names: 23097925

Gene position: 498579-500129 (Clockwise)

Preceding gene: 23097924

Following gene: 23097932

Centisome position: 13.73

GC content: 37.98

Gene sequence:

>1551_bases
GTGAGATCCCAAGATAAGAAGGAACTCGATTGGCCCGTCTTTTATATAAGCGGAGGTTTTTTAATATTATTTATAATCCT
TTCATTATTAAGTAAGGATAAAGTCGGAGAGTGGGTAAATACTTTATTTACTTATTCCTCAGATTGGTTTGGAGCATATT
GGCAAATATTACTATTACTTAATTTCTTGATCGGACTAGGATTAGCTGTATCAAAATATGGAAAAGTTCGTTTAGGGAAA
ATGGATAAACCAAAATATAGTTATTTTGGTTGGGTAAGTATGATTTTGGTGACATTACTTGCGAGTGGCGGTGTTTTCTG
GGCGGCCGCTGAACCGATGTATCATTATATGGATACACCACCTTTGTTTGGTGGTGGGGAGTTTAACTACTTAGATGGTG
CAATGGCCCAAGCTTTTGTCCATTGGGGATTCCTAGCATGGGCAATTCTCGGTACTTTAGCTGTAGTAGTCATGATGTAT
GTCCATTATCACAAAGGATATCCATTAAAACCGAGAGGATTACTATATCCAATATTTGGTGAAAGAATTTTTGAAAAGAG
TGTTGTCGGTACACTTGCAGACAGCTTTTCAATTATAGCAACAGTAGCAGGGACACTAGGCCCATTAGGGTTTCTAGGTT
TACAAATTTCTTATGGATTAAATCATTTATTTGGAGTACCCAACACACTTGTGATAAGTATGTTAGTCGTGCTTGGATTA
GTTGCTGTTGCTGCTATTTCAGCGGCAACAGGTGTAGATAGAGGGATTTATCATTTAAGTAATTGGAATGTACAATTTAC
TATTCTTTTAGCATTAATTGTATTTCTTGTAGGTCCGACAACATTTATTCTTGATGCGTTTATCGGTGGAACAGGTGTTC
ATTTACAAAACTTCTTTACAATGAACTTGTTCCGTGGAGATGAAGGTTGGTTATCCGGTTGGACGATATTCTTCTGGGGT
TGGTTTATTGGCTATGCACCGATGCTAATTGTGTTTATAAGCCGGATATCTAGAGGTCGAACGATTCGTGAACTAATTAT
TGCAGTATCAGTAGTTGCTCCATTAGTTACAAATTTCTGGTTCTCTGTTGTAGGAGGAACGGGAATATTCTTTGAAAATG
AAAACTCAGGTTCTGTTTCAAATGCATTAACAGAGGGTGGAATGCCTGCGGCTGTTATGGCGATAACGGATCAATTACCG
ATGGGAATCATATTTGGAGTAGGATTTTTATTAGTATCGATTATATTTGTAGCTACTACGACGGATACAATGTCATACAC
CGTGGCTGCGACATTATCGGGTCATGATAACCCGAAACGTTGGTTGCGTGTGTTCTGGGCAATCATCTTCGGTGCTACTA
CTGCTGTGATTTTAACAATTGGTGAGGATAGCATTACATCAATACAAAATTCGATTGTTATTACTGCGGTTCCGGTATCT
TTCTTGTTATTACCACCGTTGTGGAACGCGCCAAAAATTGCAAAGAAGATGGCGTTAGAACAAGGGTTAATTTGGCAACG
TGAAATGAATAAAAAAGTAGATAAAGATTAA

Upstream 100 bases:

>100_bases
TGATTCACGTTTTCTCTGTTTTCATTTATCCATCCGACGGTCTTTATGAACAGTTAATACTAACTTTAGGCGACTTGCTG
TAAAAATCAGGAGGTAAAAC

Downstream 100 bases:

>100_bases
AGAGAAAAGACGGCCTTTATAAAGGCCGTCTTTTTCATATTCCAGGAAATTATAAAATTTTGGGGCTGTGGATAAACAAC
TAAGTTAATTAGCCACGTCC

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MRSQDKKELDWPVFYISGGFLILFIILSLLSKDKVGEWVNTLFTYSSDWFGAYWQILLLLNFLIGLGLAVSKYGKVRLGK
MDKPKYSYFGWVSMILVTLLASGGVFWAAAEPMYHYMDTPPLFGGGEFNYLDGAMAQAFVHWGFLAWAILGTLAVVVMMY
VHYHKGYPLKPRGLLYPIFGERIFEKSVVGTLADSFSIIATVAGTLGPLGFLGLQISYGLNHLFGVPNTLVISMLVVLGL
VAVAAISAATGVDRGIYHLSNWNVQFTILLALIVFLVGPTTFILDAFIGGTGVHLQNFFTMNLFRGDEGWLSGWTIFFWG
WFIGYAPMLIVFISRISRGRTIRELIIAVSVVAPLVTNFWFSVVGGTGIFFENENSGSVSNALTEGGMPAAVMAITDQLP
MGIIFGVGFLLVSIIFVATTTDTMSYTVAATLSGHDNPKRWLRVFWAIIFGATTAVILTIGEDSITSIQNSIVITAVPVS
FLLLPPLWNAPKIAKKMALEQGLIWQREMNKKVDKD

Sequences:

>Translated_516_residues
MRSQDKKELDWPVFYISGGFLILFIILSLLSKDKVGEWVNTLFTYSSDWFGAYWQILLLLNFLIGLGLAVSKYGKVRLGK
MDKPKYSYFGWVSMILVTLLASGGVFWAAAEPMYHYMDTPPLFGGGEFNYLDGAMAQAFVHWGFLAWAILGTLAVVVMMY
VHYHKGYPLKPRGLLYPIFGERIFEKSVVGTLADSFSIIATVAGTLGPLGFLGLQISYGLNHLFGVPNTLVISMLVVLGL
VAVAAISAATGVDRGIYHLSNWNVQFTILLALIVFLVGPTTFILDAFIGGTGVHLQNFFTMNLFRGDEGWLSGWTIFFWG
WFIGYAPMLIVFISRISRGRTIRELIIAVSVVAPLVTNFWFSVVGGTGIFFENENSGSVSNALTEGGMPAAVMAITDQLP
MGIIFGVGFLLVSIIFVATTTDTMSYTVAATLSGHDNPKRWLRVFWAIIFGATTAVILTIGEDSITSIQNSIVITAVPVS
FLLLPPLWNAPKIAKKMALEQGLIWQREMNKKVDKD
>Mature_516_residues
MRSQDKKELDWPVFYISGGFLILFIILSLLSKDKVGEWVNTLFTYSSDWFGAYWQILLLLNFLIGLGLAVSKYGKVRLGK
MDKPKYSYFGWVSMILVTLLASGGVFWAAAEPMYHYMDTPPLFGGGEFNYLDGAMAQAFVHWGFLAWAILGTLAVVVMMY
VHYHKGYPLKPRGLLYPIFGERIFEKSVVGTLADSFSIIATVAGTLGPLGFLGLQISYGLNHLFGVPNTLVISMLVVLGL
VAVAAISAATGVDRGIYHLSNWNVQFTILLALIVFLVGPTTFILDAFIGGTGVHLQNFFTMNLFRGDEGWLSGWTIFFWG
WFIGYAPMLIVFISRISRGRTIRELIIAVSVVAPLVTNFWFSVVGGTGIFFENENSGSVSNALTEGGMPAAVMAITDQLP
MGIIFGVGFLLVSIIFVATTTDTMSYTVAATLSGHDNPKRWLRVFWAIIFGATTAVILTIGEDSITSIQNSIVITAVPVS
FLLLPPLWNAPKIAKKMALEQGLIWQREMNKKVDKD

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=545, Percent_Identity=28.4403669724771, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1788102, Length=427, Percent_Identity=34.192037470726, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1786224, Length=494, Percent_Identity=26.1133603238866, Blast_Score=175, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56831; Mature: 56831

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MRSQDKKELDWPVFYISGGFLILFIILSLLSKDKVGEWVNTLFTYSSDWFGAYWQILLLL
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
NFLIGLGLAVSKYGKVRLGKMDKPKYSYFGWVSMILVTLLASGGVFWAAAEPMYHYMDTP
HHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCC
PLFGGGEFNYLDGAMAQAFVHWGFLAWAILGTLAVVVMMYVHYHKGYPLKPRGLLYPIFG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHH
ERIFEKSVVGTLADSFSIIATVAGTLGPLGFLGLQISYGLNHLFGVPNTLVISMLVVLGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHH
VAVAAISAATGVDRGIYHLSNWNVQFTILLALIVFLVGPTTFILDAFIGGTGVHLQNFFT
HHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHEEE
MNLFRGDEGWLSGWTIFFWGWFIGYAPMLIVFISRISRGRTIRELIIAVSVVAPLVTNFW
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
FSVVGGTGIFFENENSGSVSNALTEGGMPAAVMAITDQLPMGIIFGVGFLLVSIIFVATT
HHHHCCCEEEEECCCCCCHHHHHHCCCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
TDTMSYTVAATLSGHDNPKRWLRVFWAIIFGATTAVILTIGEDSITSIQNSIVITAVPVS
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCEEEEEECHH
FLLLPPLWNAPKIAKKMALEQGLIWQREMNKKVDKD
HHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]