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The map label for this gene is hemC

Identifier: 229918358

GI number: 229918358

Start: 2609865

End: 2610782

Strand: Direct

Name: hemC

Synonym: EAT1b_2643

Alternate gene names: 229918358

Gene position: 2609865-2610782 (Clockwise)

Preceding gene: 229918357

Following gene: 229918359

Centisome position: 87.0

GC content: 53.7

Gene sequence:

>918_bases
ATGCGGAAAATCATTGTCGGCTCACGCCGTTCCCAACTCGCCATGACTCAAACAAAATGGGTCATCGAGCAATTGAAACA
AGCTGGAGCAACAAACGAGTTCGAGATTAAAGAAATCGTGACGAAAGGGGACCAAATCGTCGACGTGCAATTGTCGAAAG
TCGGGGGGAAAGGTCTGTTCGTCAAAGAGATTCAACAACAGATGCTCGATGGGGACATCGATTTCGCCGTACACAGTATG
AAAGACGTTCCGGCCGAACTACCAGAAGAACTCTATATCAGCTGCATGCCGAAACGTGTCGATGCGCGTGACGTCCTCAT
CTGTGAGAACGGCTACACGTTCGAGACGCTTCCGAAAGGTTCAATCGTAGGAACGAGTTCACTCCGTCGTGGTGCTCAGA
TCTTGGCAGCACGTCCTGACCTTGAGATTCAATGGATCCGTGGAAACATCGATACGCGTCTGAAAAAGTTAGACTCGGAG
AACTACAACGCCATCTTGCTCGCGAAAGCAGGACTCGAGCGGATGGGGTGGACAGATCGTGTCGACTTTGAAGCGCTCGA
CCCGGACGTCATGGTACCGGCTGTCGGACAAGGCGTACTCGCCATCGAAAGCCGCAAGGACGATGCAGAAGTGACAACGG
TGCTTCAATTATTGCATGATGATGTGACGGCGAAAGCGGCGACGGCGGAGCGTACCTTCTTGAAAGCCATCGAAGGAAGC
TGTCACGTTCCGGTCGGCGGTTATGCGACGGTCAACGGTGACGATGTGACCCTCACAGGCCTCATCGCGTCAGTGGACGG
GAAAGAAGTGCTCCGTGTGACGAAGACGTCAACGGACGGCGTTGCACTCGGACAAGCAGTCGCAGAAGAATTGCTCGGTC
GTGGCGGGCGTGAAATCCTCGCCTCGGTCAATGAGTGA

Upstream 100 bases:

>100_bases
ACAGATGCCACTTCTGCCAAAGCACCACTTCGCGCATTGATGCGCTAACAAGACAAAAAGGCGACGCGTGTCGCCTTTTT
CTATGAAGGAGAGATTGAAC

Downstream 100 bases:

>100_bases
CGTATGGTACACGGGAACGAAACGAATGACCGCTTCGCAACGGGCACGGCTCGAGGCGAGCGGTCTTTCCGTTCATCATG
TCCCGCTCATTCAGACGAAA

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRKIIVGSRRSQLAMTQTKWVIEQLKQAGATNEFEIKEIVTKGDQIVDVQLSKVGGKGLFVKEIQQQMLDGDIDFAVHSM
KDVPAELPEELYISCMPKRVDARDVLICENGYTFETLPKGSIVGTSSLRRGAQILAARPDLEIQWIRGNIDTRLKKLDSE
NYNAILLAKAGLERMGWTDRVDFEALDPDVMVPAVGQGVLAIESRKDDAEVTTVLQLLHDDVTAKAATAERTFLKAIEGS
CHVPVGGYATVNGDDVTLTGLIASVDGKEVLRVTKTSTDGVALGQAVAEELLGRGGREILASVNE

Sequences:

>Translated_305_residues
MRKIIVGSRRSQLAMTQTKWVIEQLKQAGATNEFEIKEIVTKGDQIVDVQLSKVGGKGLFVKEIQQQMLDGDIDFAVHSM
KDVPAELPEELYISCMPKRVDARDVLICENGYTFETLPKGSIVGTSSLRRGAQILAARPDLEIQWIRGNIDTRLKKLDSE
NYNAILLAKAGLERMGWTDRVDFEALDPDVMVPAVGQGVLAIESRKDDAEVTTVLQLLHDDVTAKAATAERTFLKAIEGS
CHVPVGGYATVNGDDVTLTGLIASVDGKEVLRVTKTSTDGVALGQAVAEELLGRGGREILASVNE
>Mature_305_residues
MRKIIVGSRRSQLAMTQTKWVIEQLKQAGATNEFEIKEIVTKGDQIVDVQLSKVGGKGLFVKEIQQQMLDGDIDFAVHSM
KDVPAELPEELYISCMPKRVDARDVLICENGYTFETLPKGSIVGTSSLRRGAQILAARPDLEIQWIRGNIDTRLKKLDSE
NYNAILLAKAGLERMGWTDRVDFEALDPDVMVPAVGQGVLAIESRKDDAEVTTVLQLLHDDVTAKAATAERTFLKAIEGS
CHVPVGGYATVNGDDVTLTGLIASVDGKEVLRVTKTSTDGVALGQAVAEELLGRGGREILASVNE

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=282, Percent_Identity=43.2624113475177, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI66933009, Length=282, Percent_Identity=43.2624113475177, Blast_Score=223, Evalue=1e-58,
Organism=Escherichia coli, GI48994974, Length=300, Percent_Identity=44.3333333333333, Blast_Score=244, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6319996, Length=316, Percent_Identity=34.8101265822785, Blast_Score=182, Evalue=8e-47,
Organism=Drosophila melanogaster, GI20130425, Length=283, Percent_Identity=43.1095406360424, Blast_Score=217, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_EXISA (C4L4J6)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887004.1
- GeneID:   7869656
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2643
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 33161; Mature: 33161

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIVGSRRSQLAMTQTKWVIEQLKQAGATNEFEIKEIVTKGDQIVDVQLSKVGGKGLF
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCEE
VKEIQQQMLDGDIDFAVHSMKDVPAELPEELYISCMPKRVDARDVLICENGYTFETLPKG
HHHHHHHHHCCCHHHHHHHHHCCCHHCCHHHHHEECCCCCCCCEEEEECCCCEEEECCCC
SIVGTSSLRRGAQILAARPDLEIQWIRGNIDTRLKKLDSENYNAILLAKAGLERMGWTDR
CEEECHHHHCCHHEEEECCCCEEEEEECCHHHHHHHCCCCCCCEEEEEECCHHHCCCCCC
VDFEALDPDVMVPAVGQGVLAIESRKDDAEVTTVLQLLHDDVTAKAATAERTFLKAIEGS
CCCEECCCCEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
CHVPVGGYATVNGDDVTLTGLIASVDGKEVLRVTKTSTDGVALGQAVAEELLGRGGREIL
CCCCCCCEEEECCCCEEEEEEEEECCCHHEEEEECCCCCCHHHHHHHHHHHHCCCCHHHH
ASVNE
HCCCC
>Mature Secondary Structure
MRKIIVGSRRSQLAMTQTKWVIEQLKQAGATNEFEIKEIVTKGDQIVDVQLSKVGGKGLF
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCEE
VKEIQQQMLDGDIDFAVHSMKDVPAELPEELYISCMPKRVDARDVLICENGYTFETLPKG
HHHHHHHHHCCCHHHHHHHHHCCCHHCCHHHHHEECCCCCCCCEEEEECCCCEEEECCCC
SIVGTSSLRRGAQILAARPDLEIQWIRGNIDTRLKKLDSENYNAILLAKAGLERMGWTDR
CEEECHHHHCCHHEEEECCCCEEEEEECCHHHHHHHCCCCCCCEEEEEECCHHHCCCCCC
VDFEALDPDVMVPAVGQGVLAIESRKDDAEVTTVLQLLHDDVTAKAATAERTFLKAIEGS
CCCEECCCCEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
CHVPVGGYATVNGDDVTLTGLIASVDGKEVLRVTKTSTDGVALGQAVAEELLGRGGREIL
CCCCCCCEEEECCCCEEEEEEEEECCCHHEEEEECCCCCCHHHHHHHHHHHHCCCCHHHH
ASVNE
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA