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The map label for this gene is menE [H]

Identifier: 229918091

GI number: 229918091

Start: 2323113

End: 2324471

Strand: Reverse

Name: menE [H]

Synonym: EAT1b_2371

Alternate gene names: 229918091

Gene position: 2324471-2323113 (Counterclockwise)

Preceding gene: 229918092

Following gene: 229918090

Centisome position: 77.49

GC content: 52.17

Gene sequence:

>1359_bases
ATGTATGAATGGATTCAGAAACAGCAAGCGTCACATATCGCCCTTGTGACAGCTGACGATCGATTGACATGGGCAATGCT
ACGCACGGAAAGCCTTCAACTCGCCCAGTTCATCCATGAACAGACGCCAACTGCCGAACGGATTGGCATATTCGCTAAAA
ACAGCAAAGACTATGTCATCGCCGTCCATGCGGTGCATCTGCTCGGAAAAGTGCTTGTGCCGTTGAACACACGGCTGACC
GAAGAAGAAGTGCGCACTCAGTTACAAACAGCAAAAGTGGACCTCCTCCTCGTCGACGCAGAAATCGATTTGTCCGTCAC
CCAACTTCTGATCACAGATCGGGCCACTTCATGTTTCAACGCGCCCCATGAGTGGAAAGAAGATGAAACCATGTCGCTCA
TGTTCACGTCCGGTACGACCGGTCGAGCCAAAGCAGTGCAACAGACGTACGGGAATCACCTCGCTAGCGCCCGTGCCGCT
GAAGCGCATTTAGACTATCGTCCATCAGACCGGATGCTCGCGGTGACCCCGCTCTTTCATATGAGCGGACTCTCCCAAGT
GTATCGGAGTGCCATCATCGGTTCGACGCTCTATGTCGAGCCGCGATTTGATGTCGATCGAACCCTTCACGTCATCGAAA
CAGAAGGCATCACCCAACTGTCACTCGTCTCAATTATGCTGCAACGATTGCTCGATGCCGGCCTGAAGAAACATCATCTT
CGCACGTTACTCGTCGGAGGTGGACCCGTCCCACGCTCATTACTAGAAGAAGCAGAACGACGGCAGTTACCCATTGCTCA
GACATACGGGATGACCGAGACGTGCTCTCAGGTCGCGACGTTACTTCCGAGCGAAGCGCTCTCACATATCGGTTCGAGTG
GGCAGGCAATCGCCCCAACAAGAATTCGAATTAATTCAGACGGAGAGATTGAAGTAAAGGGACCAACTGTGACGAAAGGA
TACTTCGAACAACCTGAATCAGATGCTTGGACACCCGATGGTTATTGGCGAACGGGTGACCTCGGCACGATGAGGGATGG
CTATTTATATGTACACGATCGCCGAACCGACCTTATTCTGTCTGGTGGTGAAAATGTTTATCCGGCTGAAATCGAAGCTG
CCATGTTACGCTGTACCGGTGTCCAAGACGTCGGCGTCACGAAACAATCACATCCGACTTGGGGAGAAGTCCCAATCGCC
TTCGTCGTCGGCTCCTATGACCCAGCCGAGATGCAGGAAGTTCTCGATTCACAGTTGGCTAAATATAAGCACCCGATCGC
CATCCACCACGCACCGAATTTACCACGAAACGCGAACGGAAAATTAATGCGCCATCGCTTAAAGGAGCTACTGCCATGA

Upstream 100 bases:

>100_bases
TTCAAAGAAAAACGTGACCCTGACTTCGGTCAATTCCCACGTTTCCCTTAATTGTTGAGAGAGTAGCGATTTCGCTACTC
TTTTTTTGGAGGTGACCGCT

Downstream 100 bases:

>100_bases
TCAAACGTGCTGACCTTTCCATCGTCCCTTTGACTTTTAAACACCCGATCGTGACGGCACACGCCGTCTTATCTGTCCGA
AGAACGCTCATCCTTCGTCT

Product: O-succinylbenzoate-CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MYEWIQKQQASHIALVTADDRLTWAMLRTESLQLAQFIHEQTPTAERIGIFAKNSKDYVIAVHAVHLLGKVLVPLNTRLT
EEEVRTQLQTAKVDLLLVDAEIDLSVTQLLITDRATSCFNAPHEWKEDETMSLMFTSGTTGRAKAVQQTYGNHLASARAA
EAHLDYRPSDRMLAVTPLFHMSGLSQVYRSAIIGSTLYVEPRFDVDRTLHVIETEGITQLSLVSIMLQRLLDAGLKKHHL
RTLLVGGGPVPRSLLEEAERRQLPIAQTYGMTETCSQVATLLPSEALSHIGSSGQAIAPTRIRINSDGEIEVKGPTVTKG
YFEQPESDAWTPDGYWRTGDLGTMRDGYLYVHDRRTDLILSGGENVYPAEIEAAMLRCTGVQDVGVTKQSHPTWGEVPIA
FVVGSYDPAEMQEVLDSQLAKYKHPIAIHHAPNLPRNANGKLMRHRLKELLP

Sequences:

>Translated_452_residues
MYEWIQKQQASHIALVTADDRLTWAMLRTESLQLAQFIHEQTPTAERIGIFAKNSKDYVIAVHAVHLLGKVLVPLNTRLT
EEEVRTQLQTAKVDLLLVDAEIDLSVTQLLITDRATSCFNAPHEWKEDETMSLMFTSGTTGRAKAVQQTYGNHLASARAA
EAHLDYRPSDRMLAVTPLFHMSGLSQVYRSAIIGSTLYVEPRFDVDRTLHVIETEGITQLSLVSIMLQRLLDAGLKKHHL
RTLLVGGGPVPRSLLEEAERRQLPIAQTYGMTETCSQVATLLPSEALSHIGSSGQAIAPTRIRINSDGEIEVKGPTVTKG
YFEQPESDAWTPDGYWRTGDLGTMRDGYLYVHDRRTDLILSGGENVYPAEIEAAMLRCTGVQDVGVTKQSHPTWGEVPIA
FVVGSYDPAEMQEVLDSQLAKYKHPIAIHHAPNLPRNANGKLMRHRLKELLP
>Mature_452_residues
MYEWIQKQQASHIALVTADDRLTWAMLRTESLQLAQFIHEQTPTAERIGIFAKNSKDYVIAVHAVHLLGKVLVPLNTRLT
EEEVRTQLQTAKVDLLLVDAEIDLSVTQLLITDRATSCFNAPHEWKEDETMSLMFTSGTTGRAKAVQQTYGNHLASARAA
EAHLDYRPSDRMLAVTPLFHMSGLSQVYRSAIIGSTLYVEPRFDVDRTLHVIETEGITQLSLVSIMLQRLLDAGLKKHHL
RTLLVGGGPVPRSLLEEAERRQLPIAQTYGMTETCSQVATLLPSEALSHIGSSGQAIAPTRIRINSDGEIEVKGPTVTKG
YFEQPESDAWTPDGYWRTGDLGTMRDGYLYVHDRRTDLILSGGENVYPAEIEAAMLRCTGVQDVGVTKQSHPTWGEVPIA
FVVGSYDPAEMQEVLDSQLAKYKHPIAIHHAPNLPRNANGKLMRHRLKELLP

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=518, Percent_Identity=25.2895752895753, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI187761343, Length=518, Percent_Identity=25.2895752895753, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI38505220, Length=451, Percent_Identity=26.3858093126386, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI42544132, Length=492, Percent_Identity=23.9837398373984, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI122937307, Length=465, Percent_Identity=24.5161290322581, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI157311624, Length=465, Percent_Identity=23.6559139784946, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI157311622, Length=465, Percent_Identity=23.6559139784946, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI58082049, Length=462, Percent_Identity=23.1601731601732, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI156151445, Length=353, Percent_Identity=25.4957507082153, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI115511026, Length=454, Percent_Identity=23.7885462555066, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI8923896, Length=369, Percent_Identity=26.0162601626016, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI253970489, Length=369, Percent_Identity=25.7452574525745, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI145693145, Length=377, Percent_Identity=29.1777188328912, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1788595, Length=443, Percent_Identity=26.410835214447, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=25.1865671641791, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI221142682, Length=463, Percent_Identity=25.4859611231102, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786810, Length=351, Percent_Identity=24.2165242165242, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=480, Percent_Identity=28.125, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=468, Percent_Identity=27.1367521367521, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=510, Percent_Identity=26.4705882352941, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=362, Percent_Identity=28.1767955801105, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=359, Percent_Identity=28.9693593314763, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=521, Percent_Identity=23.0326295585413, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=521, Percent_Identity=23.0326295585413, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=521, Percent_Identity=23.0326295585413, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=507, Percent_Identity=25.0493096646943, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=353, Percent_Identity=26.9121813031161, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI25141359, Length=435, Percent_Identity=23.9080459770115, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=361, Percent_Identity=26.3157894736842, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=355, Percent_Identity=27.3239436619718, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=352, Percent_Identity=25.2840909090909, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17556552, Length=476, Percent_Identity=24.5798319327731, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=382, Percent_Identity=23.8219895287958, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=382, Percent_Identity=23.8219895287958, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17553312, Length=172, Percent_Identity=33.1395348837209, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=472, Percent_Identity=21.6101694915254, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324893, Length=185, Percent_Identity=27.5675675675676, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355181, Length=466, Percent_Identity=26.6094420600858, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18859661, Length=504, Percent_Identity=27.3809523809524, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI62472339, Length=382, Percent_Identity=25.6544502617801, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24667959, Length=382, Percent_Identity=25.6544502617801, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24667955, Length=382, Percent_Identity=25.6544502617801, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356947, Length=490, Percent_Identity=25.3061224489796, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20130357, Length=353, Percent_Identity=26.628895184136, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653035, Length=492, Percent_Identity=24.1869918699187, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI161076582, Length=497, Percent_Identity=23.7424547283702, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648257, Length=507, Percent_Identity=23.0769230769231, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24581924, Length=356, Percent_Identity=24.438202247191, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648260, Length=353, Percent_Identity=24.929178470255, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50326; Mature: 50326

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEWIQKQQASHIALVTADDRLTWAMLRTESLQLAQFIHEQTPTAERIGIFAKNSKDYVI
CCHHHHHHCCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCEEE
AVHAVHLLGKVLVPLNTRLTEEEVRTQLQTAKVDLLLVDAEIDLSVTQLLITDRATSCFN
EHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHCCHHHCC
APHEWKEDETMSLMFTSGTTGRAKAVQQTYGNHLASARAAEAHLDYRPSDRMLAVTPLFH
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHH
MSGLSQVYRSAIIGSTLYVEPRFDVDRTLHVIETEGITQLSLVSIMLQRLLDAGLKKHHL
HHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCHHHHCE
RTLLVGGGPVPRSLLEEAERRQLPIAQTYGMTETCSQVATLLPSEALSHIGSSGQAIAPT
EEEEECCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCHHHHHHHCCCCCCEECCE
RIRINSDGEIEVKGPTVTKGYFEQPESDAWTPDGYWRTGDLGTMRDGYLYVHDRRTDLIL
EEEECCCCEEEECCCEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCEEEEE
SGGENVYPAEIEAAMLRCTGVQDVGVTKQSHPTWGEVPIAFVVGSYDPAEMQEVLDSQLA
ECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
KYKHPIAIHHAPNLPRNANGKLMRHRLKELLP
HHCCCEEEEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MYEWIQKQQASHIALVTADDRLTWAMLRTESLQLAQFIHEQTPTAERIGIFAKNSKDYVI
CCHHHHHHCCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCEEE
AVHAVHLLGKVLVPLNTRLTEEEVRTQLQTAKVDLLLVDAEIDLSVTQLLITDRATSCFN
EHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHCCHHHCC
APHEWKEDETMSLMFTSGTTGRAKAVQQTYGNHLASARAAEAHLDYRPSDRMLAVTPLFH
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHH
MSGLSQVYRSAIIGSTLYVEPRFDVDRTLHVIETEGITQLSLVSIMLQRLLDAGLKKHHL
HHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCHHHHCE
RTLLVGGGPVPRSLLEEAERRQLPIAQTYGMTETCSQVATLLPSEALSHIGSSGQAIAPT
EEEEECCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCHHHHHHHCCCCCCEECCE
RIRINSDGEIEVKGPTVTKGYFEQPESDAWTPDGYWRTGDLGTMRDGYLYVHDRRTDLIL
EEEECCCCEEEECCCEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCEEEEE
SGGENVYPAEIEAAMLRCTGVQDVGVTKQSHPTWGEVPIAFVVGSYDPAEMQEVLDSQLA
ECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
KYKHPIAIHHAPNLPRNANGKLMRHRLKELLP
HHCCCEEEEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA