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The map label for this gene is menC [H]

Identifier: 229918090

GI number: 229918090

Start: 2322046

End: 2323116

Strand: Reverse

Name: menC [H]

Synonym: EAT1b_2370

Alternate gene names: 229918090

Gene position: 2323116-2322046 (Counterclockwise)

Preceding gene: 229918091

Following gene: 229918084

Centisome position: 77.44

GC content: 49.86

Gene sequence:

>1071_bases
ATGATCAAACGTGCTGACCTTTCCATCGTCCCTTTGACTTTTAAACACCCGATCGTGACGGCACACGCCGTCTTATCTGT
CCGAAGAACGCTCATCCTTCGTCTAGAGACCGTAGACGGTGTTGTCGGATACGGAGAAGGTGCTGCTTTTGAGACCCCTT
GGTACACGGAAGAAACGACCCAATCGATGATTGATACGTCCAAATTGATTCTCAATTTACTGAAAGGGATGACGGTCACG
ACGGACGTGTTTCCGAACTTGGTCTCATCTATCATCGGTCAGCGCATGGCAAAGGCGATGTGGGAAAGTGCGCTTTGGGA
AATTGAAGCGACGCGTGCGAACAAATCACTCAAGTCATTCATTAGCGCCGGAGATTCCGTCTCTTGTGGACGAACAATCG
GGATCGGGTTGCTGAATCAGACGATGAAATCCATTGAAACGGCATTAGCTGACGGATTCGAACGCATTAAATTAAAGGCA
TCGCCTAATGAACTGCTTCCTGCCCTACGTGAGATTCGCGCGGTCTTCCCTGATGCGCCCCTCATGATTGATTTAAACGG
AAGCGCGTCCGATCAACCGATGGAATGGTTCGAACAACTCGACACCTATCGGTTATTGATGATCGAACAACCGTACCCGA
GCCGACAATTCACAAAATCAGCGGCTCTCCAAGCAAAACTCACGACCCCCATTTGTCTCGACGAATCGATCACGTCTTAT
GACGACGTGGAAACGATGGCACAGCTCGGCGCGGGGAAGATTGTCAACATTAAACCCGCACGTGTCGGTGGGCTTAGCGA
GGCGTTCCACATTCGAAAACACGGAACGCCATACTGGGTCGGTGGGATGTATGAATCCGGTATCGGAAGATATCATACGC
TCTTGTTTGCTTCGTTAAGTGGCGCGAGCTTCCCTGCGGACATGTCGGGTACGAGCGCTTATTTCGAGCAAGATTTACTC
GATATCCCGCTTGAAGTGGAGAATGGAAAGTTGACGCTTCCGGATTCCGTACAACCAGATTGGAATGTGATTGAACGGTT
ACGCGAAGAAGTGATTACATTGCAAACATGA

Upstream 100 bases:

>100_bases
TGGCTAAATATAAGCACCCGATCGCCATCCACCACGCACCGAATTTACCACGAAACGCGAACGGAAAATTAATGCGCCAT
CGCTTAAAGGAGCTACTGCC

Downstream 100 bases:

>100_bases
AAAAAGGTGCGGTTCACGAGAACCACACCTTTTTACGTTAACGGAACGATCCCGTTAACACTTCATATCCATCTTCTGTG
ACAACAACTGTATGCTCGAA

Product: Mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; o-succinylbenzoic acid synthase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MIKRADLSIVPLTFKHPIVTAHAVLSVRRTLILRLETVDGVVGYGEGAAFETPWYTEETTQSMIDTSKLILNLLKGMTVT
TDVFPNLVSSIIGQRMAKAMWESALWEIEATRANKSLKSFISAGDSVSCGRTIGIGLLNQTMKSIETALADGFERIKLKA
SPNELLPALREIRAVFPDAPLMIDLNGSASDQPMEWFEQLDTYRLLMIEQPYPSRQFTKSAALQAKLTTPICLDESITSY
DDVETMAQLGAGKIVNIKPARVGGLSEAFHIRKHGTPYWVGGMYESGIGRYHTLLFASLSGASFPADMSGTSAYFEQDLL
DIPLEVENGKLTLPDSVQPDWNVIERLREEVITLQT

Sequences:

>Translated_356_residues
MIKRADLSIVPLTFKHPIVTAHAVLSVRRTLILRLETVDGVVGYGEGAAFETPWYTEETTQSMIDTSKLILNLLKGMTVT
TDVFPNLVSSIIGQRMAKAMWESALWEIEATRANKSLKSFISAGDSVSCGRTIGIGLLNQTMKSIETALADGFERIKLKA
SPNELLPALREIRAVFPDAPLMIDLNGSASDQPMEWFEQLDTYRLLMIEQPYPSRQFTKSAALQAKLTTPICLDESITSY
DDVETMAQLGAGKIVNIKPARVGGLSEAFHIRKHGTPYWVGGMYESGIGRYHTLLFASLSGASFPADMSGTSAYFEQDLL
DIPLEVENGKLTLPDSVQPDWNVIERLREEVITLQT
>Mature_356_residues
MIKRADLSIVPLTFKHPIVTAHAVLSVRRTLILRLETVDGVVGYGEGAAFETPWYTEETTQSMIDTSKLILNLLKGMTVT
TDVFPNLVSSIIGQRMAKAMWESALWEIEATRANKSLKSFISAGDSVSCGRTIGIGLLNQTMKSIETALADGFERIKLKA
SPNELLPALREIRAVFPDAPLMIDLNGSASDQPMEWFEQLDTYRLLMIEQPYPSRQFTKSAALQAKLTTPICLDESITSY
DDVETMAQLGAGKIVNIKPARVGGLSEAFHIRKHGTPYWVGGMYESGIGRYHTLLFASLSGASFPADMSGTSAYFEQDLL
DIPLEVENGKLTLPDSVQPDWNVIERLREEVITLQT

Specific function: Converts SHCHC to OSB [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=252, Percent_Identity=28.1746031746032, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- InterPro:   IPR010197 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.113 [H]

Molecular weight: Translated: 39306; Mature: 39306

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRADLSIVPLTFKHPIVTAHAVLSVRRTLILRLETVDGVVGYGEGAAFETPWYTEETT
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEEHHCCEEECCCCCCCCCCCCCHHHH
QSMIDTSKLILNLLKGMTVTTDVFPNLVSSIIGQRMAKAMWESALWEIEATRANKSLKSF
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
ISAGDSVSCGRTIGIGLLNQTMKSIETALADGFERIKLKASPNELLPALREIRAVFPDAP
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHCCCCC
LMIDLNGSASDQPMEWFEQLDTYRLLMIEQPYPSRQFTKSAALQAKLTTPICLDESITSY
EEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEEECCCCEEECCCCCCH
DDVETMAQLGAGKIVNIKPARVGGLSEAFHIRKHGTPYWVGGMYESGIGRYHTLLFASLS
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHHCC
GASFPADMSGTSAYFEQDLLDIPLEVENGKLTLPDSVQPDWNVIERLREEVITLQT
CCCCCCCCCCCHHHHHHHHHCCCEEECCCEEECCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKRADLSIVPLTFKHPIVTAHAVLSVRRTLILRLETVDGVVGYGEGAAFETPWYTEETT
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEEHHCCEEECCCCCCCCCCCCCHHHH
QSMIDTSKLILNLLKGMTVTTDVFPNLVSSIIGQRMAKAMWESALWEIEATRANKSLKSF
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
ISAGDSVSCGRTIGIGLLNQTMKSIETALADGFERIKLKASPNELLPALREIRAVFPDAP
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHCCCCC
LMIDLNGSASDQPMEWFEQLDTYRLLMIEQPYPSRQFTKSAALQAKLTTPICLDESITSY
EEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHEEECCCCEEECCCCCCH
DDVETMAQLGAGKIVNIKPARVGGLSEAFHIRKHGTPYWVGGMYESGIGRYHTLLFASLS
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHHCC
GASFPADMSGTSAYFEQDLLDIPLEVENGKLTLPDSVQPDWNVIERLREEVITLQT
CCCCCCCCCCCHHHHHHHHHCCCEEECCCEEECCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10194342 [H]