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The map label for this gene is lutB

Identifier: 229917683

GI number: 229917683

Start: 1920332

End: 1921738

Strand: Reverse

Name: lutB

Synonym: EAT1b_1960

Alternate gene names: 229917683

Gene position: 1921738-1920332 (Counterclockwise)

Preceding gene: 229917684

Following gene: 229917682

Centisome position: 64.06

GC content: 53.09

Gene sequence:

>1407_bases
ATGAGCATGGGGATTCGATTGGATGAGAAGTTTCAAGATCGACGAAAAGATGGAATTGAAGATGCGTTCATGCGGCAGGC
CGTCAGTTCGGCTCAAAACCGTCTACGGGATGGCCGGTTGAATGCTGCAGGTGAACTCGGGGACTGGGAAGCGTTTCGCG
ACCATTCGGAAGAAATTCGTCAACATGTGCTGGAACATCTGGATTATTACTTATATCAATTGAGCGAGAATGTCTCAAAA
CGTGGTGGAAGCGTCTTTTTCGCGGAGACACCGGAAGAAGCGAACCGCTACATCCGGGAAGTCATCCGGTCGAAACAAGC
CAAACATGTCGTGAAATCGAAATCGATGGTCACGGAAGAAATCGGCTTGAACGAGGCACTTCAGGAAGAAGGATGTACCG
TCGTCGAGTCCGATCTTGGGGAATGGATTTTGCAACTCGACGAAGACCCACCGTCTCACATCGTCGCCCCGGCTCTGCAT
AAAGACCGCAACGCTGTCCATGAGACGTTCACTGCTCACGGCTATACCGGTTCAAACGATCCGACTGAACTCGGTCGTTA
TGCGCGTCAAGAACTGCGCAAAGACTTTTTGAAGGCAGAAGTCGGCATCACCGGCTGTAATTTCGCCATCGCAGAGAGTG
GAGCCGTCGGGCTTGTGACCAATGAAGGGAACGGACGCATGGTGACGTCGCTTCCTGATACGGTCATCTCTGTCATGGGG
ATGGAACGCGTCGTCCCGTCTTATGAAGAGTTCGAGGTGCTCGTCAATATGCTTTGTCGAAGCGCGGTCGGTCAGCGACT
CACGTCATACATCACGACATTCGCCGGGACGCGCGCGGTCGGTGAAGTCGATGGACCAGAAGAGTTCCACCTCGTCATCG
TCGACAACAAGCGTTCTAATATTCTCGGCACACAGTTCCAATCGGTCCTGCAATGCATCCGTTGTGCGGCCTGTATCAAC
GTCTGTCCCGTCTATCGTCACATCGGTGGTCATGCGTACGGGTCGATCTATCCAGGTCCGATTGGTGCCGTCTTAGCCCC
ACTGCTAGATGGTTACGAGAACTATAAAGAACTCCCCTATGCGTCGAGTCTCTGTGGCGCCTGTACGGAGGCGTGTCCTG
TAAAGATTCCGCTGCATGAGCTGTTGATTGAACATCGACGCGTCATCGTAGAGGAGAAAAAGCAATCACCTTTCGTGGAG
CGGATTGCGATGAAAGGATTTGCCGTCACCGCTTCGAGCCCATTCTTATTCGAAGCAGCGGAACATGTCGCACCGTTTGC
GACATCACCATTCGCCACCTCGGGTCAGATTGAGAAAGGGCTACCGGCTTGGACGGACTCGAAAGACTTGCCACAGCCGA
ACAAACAGACCGTCCGAGATTGGTTCAAGAAACGGGGGAATGCCTGA

Upstream 100 bases:

>100_bases
CGTCATGTTTGATGAACATCGGCGGACGTCTTCATAAACAGGGACATCCGATTAAAGTGATGCATATTGCCGAAGTCTTG
AACGAAGGAGTGAAGCAAGC

Downstream 100 bases:

>100_bases
TGAATCCTGGTACAATCACAAACCGTGAGGACTTTCTACAACGAATCGCCAAGCAACTCGGTCGTGAGGTCAAGCTCACG
CCGCCAAAACGGGAATATAC

Product: iron-sulfur cluster binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSMGIRLDEKFQDRRKDGIEDAFMRQAVSSAQNRLRDGRLNAAGELGDWEAFRDHSEEIRQHVLEHLDYYLYQLSENVSK
RGGSVFFAETPEEANRYIREVIRSKQAKHVVKSKSMVTEEIGLNEALQEEGCTVVESDLGEWILQLDEDPPSHIVAPALH
KDRNAVHETFTAHGYTGSNDPTELGRYARQELRKDFLKAEVGITGCNFAIAESGAVGLVTNEGNGRMVTSLPDTVISVMG
MERVVPSYEEFEVLVNMLCRSAVGQRLTSYITTFAGTRAVGEVDGPEEFHLVIVDNKRSNILGTQFQSVLQCIRCAACIN
VCPVYRHIGGHAYGSIYPGPIGAVLAPLLDGYENYKELPYASSLCGACTEACPVKIPLHELLIEHRRVIVEEKKQSPFVE
RIAMKGFAVTASSPFLFEAAEHVAPFATSPFATSGQIEKGLPAWTDSKDLPQPNKQTVRDWFKKRGNA

Sequences:

>Translated_468_residues
MSMGIRLDEKFQDRRKDGIEDAFMRQAVSSAQNRLRDGRLNAAGELGDWEAFRDHSEEIRQHVLEHLDYYLYQLSENVSK
RGGSVFFAETPEEANRYIREVIRSKQAKHVVKSKSMVTEEIGLNEALQEEGCTVVESDLGEWILQLDEDPPSHIVAPALH
KDRNAVHETFTAHGYTGSNDPTELGRYARQELRKDFLKAEVGITGCNFAIAESGAVGLVTNEGNGRMVTSLPDTVISVMG
MERVVPSYEEFEVLVNMLCRSAVGQRLTSYITTFAGTRAVGEVDGPEEFHLVIVDNKRSNILGTQFQSVLQCIRCAACIN
VCPVYRHIGGHAYGSIYPGPIGAVLAPLLDGYENYKELPYASSLCGACTEACPVKIPLHELLIEHRRVIVEEKKQSPFVE
RIAMKGFAVTASSPFLFEAAEHVAPFATSPFATSGQIEKGLPAWTDSKDLPQPNKQTVRDWFKKRGNA
>Mature_467_residues
SMGIRLDEKFQDRRKDGIEDAFMRQAVSSAQNRLRDGRLNAAGELGDWEAFRDHSEEIRQHVLEHLDYYLYQLSENVSKR
GGSVFFAETPEEANRYIREVIRSKQAKHVVKSKSMVTEEIGLNEALQEEGCTVVESDLGEWILQLDEDPPSHIVAPALHK
DRNAVHETFTAHGYTGSNDPTELGRYARQELRKDFLKAEVGITGCNFAIAESGAVGLVTNEGNGRMVTSLPDTVISVMGM
ERVVPSYEEFEVLVNMLCRSAVGQRLTSYITTFAGTRAVGEVDGPEEFHLVIVDNKRSNILGTQFQSVLQCIRCAACINV
CPVYRHIGGHAYGSIYPGPIGAVLAPLLDGYENYKELPYASSLCGACTEACPVKIPLHELLIEHRRVIVEEKKQSPFVER
IAMKGFAVTASSPFLFEAAEHVAPFATSPFATSGQIEKGLPAWTDSKDLPQPNKQTVRDWFKKRGNA

Specific function: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1786498, Length=458, Percent_Identity=54.585152838428, Blast_Score=525, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LUTB_EXISA (C4L0S1)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886329.1
- GeneID:   7869170
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1960
- OMA:   MAIADKG
- GO:   GO:0006810
- HAMAP:   MF_02103
- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825
- Gene3D:   G3DSA:3.40.50.10420
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR00273

Pfam domain/function: PF02589 DUF162; SSF46548 Helical_ferredxn

EC number: NA

Molecular weight: Translated: 51996; Mature: 51865

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMGIRLDEKFQDRRKDGIEDAFMRQAVSSAQNRLRDGRLNAAGELGDWEAFRDHSEEIR
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
QHVLEHLDYYLYQLSENVSKRGGSVFFAETPEEANRYIREVIRSKQAKHVVKSKSMVTEE
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLNEALQEEGCTVVESDLGEWILQLDEDPPSHIVAPALHKDRNAVHETFTAHGYTGSND
HCCHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
PTELGRYARQELRKDFLKAEVGITGCNFAIAESGAVGLVTNEGNGRMVTSLPDTVISVMG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEEEECCHHHHHHHHH
MERVVPSYEEFEVLVNMLCRSAVGQRLTSYITTFAGTRAVGEVDGPEEFHLVIVDNKRSN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCC
ILGTQFQSVLQCIRCAACINVCPVYRHIGGHAYGSIYPGPIGAVLAPLLDGYENYKELPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
ASSLCGACTEACPVKIPLHELLIEHRRVIVEEKKQSPFVERIAMKGFAVTASSPFLFEAA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEECCCCHHHHHH
EHVAPFATSPFATSGQIEKGLPAWTDSKDLPQPNKQTVRDWFKKRGNA
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SMGIRLDEKFQDRRKDGIEDAFMRQAVSSAQNRLRDGRLNAAGELGDWEAFRDHSEEIR
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
QHVLEHLDYYLYQLSENVSKRGGSVFFAETPEEANRYIREVIRSKQAKHVVKSKSMVTEE
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLNEALQEEGCTVVESDLGEWILQLDEDPPSHIVAPALHKDRNAVHETFTAHGYTGSND
HCCHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
PTELGRYARQELRKDFLKAEVGITGCNFAIAESGAVGLVTNEGNGRMVTSLPDTVISVMG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEEEECCHHHHHHHHH
MERVVPSYEEFEVLVNMLCRSAVGQRLTSYITTFAGTRAVGEVDGPEEFHLVIVDNKRSN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCC
ILGTQFQSVLQCIRCAACINVCPVYRHIGGHAYGSIYPGPIGAVLAPLLDGYENYKELPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
ASSLCGACTEACPVKIPLHELLIEHRRVIVEEKKQSPFVERIAMKGFAVTASSPFLFEAA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEECCCCHHHHHH
EHVAPFATSPFATSGQIEKGLPAWTDSKDLPQPNKQTVRDWFKKRGNA
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA