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The map label for this gene is 227827028

Identifier: 227827028

GI number: 227827028

Start: 639595

End: 641070

Strand: Direct

Name: 227827028

Synonym: M1425_0686

Alternate gene names: NA

Gene position: 639595-641070 (Clockwise)

Preceding gene: 227827027

Following gene: 227827029

Centisome position: 24.52

GC content: 38.35

Gene sequence:

>1476_bases
ATGATAAGAAGGGGAACAGATTACATAAAAAGTATAAAGGAAAACCCACCAGTAGTTTACTATGAAGGAGAAGTAGTAAA
GGATGTTACAGAACATTCAGCATTTAAAATACCGATATCTACTGTTGCGAAATATTATGATCTACATTGGGATGAGAAAT
ATAAGGAGTACCTAAGGATTTATAACCTTGATGTGGGAGAGGAAACTAGCATAAGTTTTCTAAGACCTAAAAACAAGAAA
GATTTAGGCAAATTAAGGGATGGCCTAGTAAAGATATATGATTACTATAGGGGATTCTTCGGTAGAAGTCCAGACTACCT
AAACTTATGGACAATGGTATTCTATGCACACGCTGAAGACTACTTTGGAAAACAGTTCGGTTCAAAGTTCATGGAAAATG
CGATTGAAATTTACAAAGAAGCTACAAAGAGAGATTTGTTCTATACTCACGCAATTGTGGCCCCAATGTACGATAGATCT
AGACCACCTTCTCAATGGGAAGATCCATACATTCAAGTGGGAATAGTTGAGGAAAAACCAGAAGGTGTAGTAGTAAGAGG
AGCCGCAATGATATGTACCGCCGGTCCTTATGCGGAGATGTTATGGTATCTACCAAATGTCAGAAGAGATAGTGATCCTA
GATATGCAATATACTTCTCTATCCCAACAAACACTAAAGGAGTTAAGTTCTTGTCTAGAAGGGGGTTCCAGCCAAGGGAA
GGAGGAGAATTCGAATACCCAATATCTTCTAGATTTGAGGAATCCGATGCAATTTTAGTGTTAGATAACGTCCTAATTCC
TTGGGAAAGGATAATATTCTTCAAGAAGCCTGAGTTAATCGAAGACCTAATGTGGCACACCGTTCAGTTAAGGGGATGGT
TTAACTGGCACTTCGTAATTCAACATTATAGCAGGTTAAAATTCCTTGCTGGTTTAGCTATCGCCATTGCTGAAGCTTCT
GGCACCAGTAATTTCATTAACGTTCAAGAAAAGATAGGTGAGATACTGATATATGTAGCTCTAAATGAGGCAGCCCTCTA
TGCATCAGTTGAAAGAGCCCAAGAATTGCCCAATATTACTAGACCAGATCCCTATATTTCCATTTCAGCCAGTCACTTCA
ACATGAAGACAGTTCCCAGAGCAAATGAGATATTAAGGTCAATTAGTGCAGGATCATCAATACCTATACCTGCTGGTATT
AAAGATTTTGAAAATCCCGAGGAAAGAAAATTATTGGATAAGTACATGGCAATGAAAGATCTTGATGCCCTAGAAAGAGT
TAAGACGTTTAACCTACTGTGGGACGTTATAGGATCTGAAGCAGGGATGAGATATGAACAATATGATAGGTTCAGTAGGG
GTGATCCCACAATAAGATGGGCTCAAACTTATACTGAGGTATTCAAGGATAGAAAGCACGAATTCGTCAAATTGGTTAAG
GATATAATCGATCAGATGCCTAATCCTAAAGCTTAA

Upstream 100 bases:

>100_bases
ATAGAAGTGATTTTTGGGGAAGGCCGATTCCTCAATCTTGGTTAACTGAATGGGTTCCCGTGGAGGATATTTCCACGGGT
CAATTGAAGGGGTGGTCTGG

Downstream 100 bases:

>100_bases
GTGAACAAAAGTTAACTTAATTTTTTATTTATATTTTTGGAGTTCTTATTAATGACAATTGGCTTATTTATATCTCATGG
TTCTCCTACAATACTGATTG

Product: 4-hydroxyphenylacetate 3-hydroxylase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVAKYYDLHWDEKYKEYLRIYNLDVGEETSISFLRPKNKK
DLGKLRDGLVKIYDYYRGFFGRSPDYLNLWTMVFYAHAEDYFGKQFGSKFMENAIEIYKEATKRDLFYTHAIVAPMYDRS
RPPSQWEDPYIQVGIVEEKPEGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPTNTKGVKFLSRRGFQPRE
GGEFEYPISSRFEESDAILVLDNVLIPWERIIFFKKPELIEDLMWHTVQLRGWFNWHFVIQHYSRLKFLAGLAIAIAEAS
GTSNFINVQEKIGEILIYVALNEAALYASVERAQELPNITRPDPYISISASHFNMKTVPRANEILRSISAGSSIPIPAGI
KDFENPEERKLLDKYMAMKDLDALERVKTFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKHEFVKLVK
DIIDQMPNPKA

Sequences:

>Translated_491_residues
MIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVAKYYDLHWDEKYKEYLRIYNLDVGEETSISFLRPKNKK
DLGKLRDGLVKIYDYYRGFFGRSPDYLNLWTMVFYAHAEDYFGKQFGSKFMENAIEIYKEATKRDLFYTHAIVAPMYDRS
RPPSQWEDPYIQVGIVEEKPEGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPTNTKGVKFLSRRGFQPRE
GGEFEYPISSRFEESDAILVLDNVLIPWERIIFFKKPELIEDLMWHTVQLRGWFNWHFVIQHYSRLKFLAGLAIAIAEAS
GTSNFINVQEKIGEILIYVALNEAALYASVERAQELPNITRPDPYISISASHFNMKTVPRANEILRSISAGSSIPIPAGI
KDFENPEERKLLDKYMAMKDLDALERVKTFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKHEFVKLVK
DIIDQMPNPKA
>Mature_491_residues
MIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVAKYYDLHWDEKYKEYLRIYNLDVGEETSISFLRPKNKK
DLGKLRDGLVKIYDYYRGFFGRSPDYLNLWTMVFYAHAEDYFGKQFGSKFMENAIEIYKEATKRDLFYTHAIVAPMYDRS
RPPSQWEDPYIQVGIVEEKPEGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPTNTKGVKFLSRRGFQPRE
GGEFEYPISSRFEESDAILVLDNVLIPWERIIFFKKPELIEDLMWHTVQLRGWFNWHFVIQHYSRLKFLAGLAIAIAEAS
GTSNFINVQEKIGEILIYVALNEAALYASVERAQELPNITRPDPYISISASHFNMKTVPRANEILRSISAGSSIPIPAGI
KDFENPEERKLLDKYMAMKDLDALERVKTFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKHEFVKLVK
DIIDQMPNPKA

Specific function: Utilizes FADH(2) supplied by hpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 57266; Mature: 57266

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVAKYYDLHWDEKYKEYLRI
CCCCCHHHHHHHHCCCCEEEECCHHHHHHHHCCEEECCHHHHHHHHHCCCCHHHHHHHHH
YNLDVGEETSISFLRPKNKKDLGKLRDGLVKIYDYYRGFFGRSPDYLNLWTMVFYAHAED
HCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
YFGKQFGSKFMENAIEIYKEATKRDLFYTHAIVAPMYDRSRPPSQWEDPYIQVGIVEEKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
EGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPTNTKGVKFLSRRGFQPRE
CCEEEECCEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCC
GGEFEYPISSRFEESDAILVLDNVLIPWERIIFFKKPELIEDLMWHTVQLRGWFNWHFVI
CCCCCCCHHCCCCCCCCEEEECCCCCCHHHEEEECCHHHHHHHHHHEEEEEEEEEHHHHH
QHYSRLKFLAGLAIAIAEASGTSNFINVQEKIGEILIYVALNEAALYASVERAQELPNIT
HHHHHHHHHHHHHHHEEECCCCCCCEEHHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCC
RPDPYISISASHFNMKTVPRANEILRSISAGSSIPIPAGIKDFENPEERKLLDKYMAMKD
CCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LDALERVKTFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKHEFVKLVK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
DIIDQMPNPKA
HHHHHCCCCCC
>Mature Secondary Structure
MIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVAKYYDLHWDEKYKEYLRI
CCCCCHHHHHHHHCCCCEEEECCHHHHHHHHCCEEECCHHHHHHHHHCCCCHHHHHHHHH
YNLDVGEETSISFLRPKNKKDLGKLRDGLVKIYDYYRGFFGRSPDYLNLWTMVFYAHAED
HCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
YFGKQFGSKFMENAIEIYKEATKRDLFYTHAIVAPMYDRSRPPSQWEDPYIQVGIVEEKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
EGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPTNTKGVKFLSRRGFQPRE
CCEEEECCEEEECCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCC
GGEFEYPISSRFEESDAILVLDNVLIPWERIIFFKKPELIEDLMWHTVQLRGWFNWHFVI
CCCCCCCHHCCCCCCCCEEEECCCCCCHHHEEEECCHHHHHHHHHHEEEEEEEEEHHHHH
QHYSRLKFLAGLAIAIAEASGTSNFINVQEKIGEILIYVALNEAALYASVERAQELPNIT
HHHHHHHHHHHHHHHEEECCCCCCCEEHHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCC
RPDPYISISASHFNMKTVPRANEILRSISAGSSIPIPAGIKDFENPEERKLLDKYMAMKD
CCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LDALERVKTFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKHEFVKLVK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
DIIDQMPNPKA
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA