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The map label for this gene is ygiD [H]

Identifier: 227827029

GI number: 227827029

Start: 641122

End: 641895

Strand: Direct

Name: ygiD [H]

Synonym: M1425_0687

Alternate gene names: 227827029

Gene position: 641122-641895 (Clockwise)

Preceding gene: 227827028

Following gene: 227827030

Centisome position: 24.58

GC content: 36.3

Gene sequence:

>774_bases
ATGACAATTGGCTTATTTATATCTCATGGTTCTCCTACAATACTGATTGATGAGAATAAGTGGAAGGACTTATTGAGAAA
AGTAGGAAAGGAAATTGAGGAGAAGTATAAACCAGAAACAATAATAGTCTCAAGTCCCCATTTCGTCTCATGGACGGAAA
CCTTCTATATTGAAAGCAGTGAGAAGCTAGAGTGTATTCAAGATTATTACGGTTTCCCAGATGAGACTTATAAATACTGT
TATAACGCGTTAAATGACACTGAACTAGTTGAAGAAATAGTGAAAAACGCTGAGGGAAAAGTAGTGAAAGATGATAAATG
GGGATTAGATCACGGTGCGTGGATTCCCCTCTTCTACATGTTTCCCGAGTATAAACCAAAAGTTGTGACAATATCAATTA
CTGGCAATTCGCCAGAATCACACTATGAAATAGGTGAAAAGATTAGGAAAGCAGTAGAGAAACTTGGAAGGAAAGCAGTG
TTTTTAGCCACTGGTTCACCAACTCATAGGTTAGACTTGTTTTACTTTAAGATAACTCCTAAACCTACAAAATTCGATGC
GATATTAATGGATCTAATAAAATCTGGTGAATTTGAGAAAATATTAAAAATTAGTGAACTTTATCCTAAGGAGTATCAGA
CTGCAATGCCAGAGGGAAATTTAAATACTCTACACATGCTCTTAGGGTATATTAAACCAAAGAAGGCTGAGATACTAGGA
TATGATACACCTTGGCCCGGAGTTAGTATGCTAGCCGCAAGCTTTTATGAGTAA

Upstream 100 bases:

>100_bases
CAAATTGGTTAAGGATATAATCGATCAGATGCCTAATCCTAAAGCTTAAGTGAACAAAAGTTAACTTAATTTTTTATTTA
TATTTTTGGAGTTCTTATTA

Downstream 100 bases:

>100_bases
AAGACTCATTTTTAGACATGCGTTTTTGTCCTAAATGCTGTTCCTTGATGAGGGTAAAAGGTAACAAAATGGTTTGTAGT
AAGTGTGGACATTCTGATCA

Product: extradiol ring-cleavage dioxygenase class III protein subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTIGLFISHGSPTILIDENKWKDLLRKVGKEIEEKYKPETIIVSSPHFVSWTETFYIESSEKLECIQDYYGFPDETYKYC
YNALNDTELVEEIVKNAEGKVVKDDKWGLDHGAWIPLFYMFPEYKPKVVTISITGNSPESHYEIGEKIRKAVEKLGRKAV
FLATGSPTHRLDLFYFKITPKPTKFDAILMDLIKSGEFEKILKISELYPKEYQTAMPEGNLNTLHMLLGYIKPKKAEILG
YDTPWPGVSMLAASFYE

Sequences:

>Translated_257_residues
MTIGLFISHGSPTILIDENKWKDLLRKVGKEIEEKYKPETIIVSSPHFVSWTETFYIESSEKLECIQDYYGFPDETYKYC
YNALNDTELVEEIVKNAEGKVVKDDKWGLDHGAWIPLFYMFPEYKPKVVTISITGNSPESHYEIGEKIRKAVEKLGRKAV
FLATGSPTHRLDLFYFKITPKPTKFDAILMDLIKSGEFEKILKISELYPKEYQTAMPEGNLNTLHMLLGYIKPKKAEILG
YDTPWPGVSMLAASFYE
>Mature_256_residues
TIGLFISHGSPTILIDENKWKDLLRKVGKEIEEKYKPETIIVSSPHFVSWTETFYIESSEKLECIQDYYGFPDETYKYCY
NALNDTELVEEIVKNAEGKVVKDDKWGLDHGAWIPLFYMFPEYKPKVVTISITGNSPESHYEIGEKIRKAVEKLGRKAVF
LATGSPTHRLDLFYFKITPKPTKFDAILMDLIKSGEFEKILKISELYPKEYQTAMPEGNLNTLHMLLGYIKPKKAEILGY
DTPWPGVSMLAASFYE

Specific function: Unknown

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DODA-type extradiol aromatic ring- opening dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI226510974, Length=171, Percent_Identity=29.2397660818713, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014436
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: NA

Molecular weight: Translated: 29603; Mature: 29472

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGLFISHGSPTILIDENKWKDLLRKVGKEIEEKYKPETIIVSSPHFVSWTETFYIESS
CEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEEECC
EKLECIQDYYGFPDETYKYCYNALNDTELVEEIVKNAEGKVVKDDKWGLDHGAWIPLFYM
CHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEE
FPEYKPKVVTISITGNSPESHYEIGEKIRKAVEKLGRKAVFLATGSPTHRLDLFYFKITP
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEECC
KPTKFDAILMDLIKSGEFEKILKISELYPKEYQTAMPEGNLNTLHMLLGYIKPKKAEILG
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEE
YDTPWPGVSMLAASFYE
CCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TIGLFISHGSPTILIDENKWKDLLRKVGKEIEEKYKPETIIVSSPHFVSWTETFYIESS
EEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEEECC
EKLECIQDYYGFPDETYKYCYNALNDTELVEEIVKNAEGKVVKDDKWGLDHGAWIPLFYM
CHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEE
FPEYKPKVVTISITGNSPESHYEIGEKIRKAVEKLGRKAVFLATGSPTHRLDLFYFKITP
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEECC
KPTKFDAILMDLIKSGEFEKILKISELYPKEYQTAMPEGNLNTLHMLLGYIKPKKAEILG
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEE
YDTPWPGVSMLAASFYE
CCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1314093; 9278503 [H]