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The map label for this gene is galF [C]

Identifier: 227826988

GI number: 227826988

Start: 591035

End: 591787

Strand: Direct

Name: galF [C]

Synonym: M1425_0645

Alternate gene names: 227826988

Gene position: 591035-591787 (Clockwise)

Preceding gene: 227826987

Following gene: 227826991

Centisome position: 22.66

GC content: 32.93

Gene sequence:

>753_bases
ATGCATGCAGTAATAACTGCAGCTGGATTAGGCACTCGAATGTTACCCGCTTCTAAAGAAATTCCTAAGGAGATGTTTCC
AATTCCCTTTAACAATGGATTTAAGCCAATTATACAAATAATCTTTGAGCAACTTTATAACAAGGGTATAAGGGATTTCG
TTATAGTTGTTGGTAGGGGTAAGAGGGTAATTGAAGATCATTTTACACCAGATTACGATTTTGTCTCTTACCTTGAAAAA
GTAGGTAAAGAAAAGCAGGCAAGGGAGTTATTAACATTTTACTCTAAAATAGAGAAAAGCAATATAGCATTTGTAAATCA
GCCTGAACCAAAAGGATTTGGAGATGCGGTACTGAGAGTAGAACCTTTTATTAATGATAAATTCATTGTAGTAGCAGCAG
ACACTCTATTAAGAGAGATTCCAGATTTAATACCTAACTCTTTTCTAGTTACTGAAGTAGAAGATCCTAGACCATATGGT
GTAGTAATATTAGAGGGAGATAGAGTTATAGATGTCGAGGAAAAACCTAAGAATCCTAAATCCCGTTTTATAATAGTTCC
TTACTATATGTTTACATATGATATATTTCACGCCTTAAGAGAAGTTAAGTGTAACGGAGAATTACAACTTACTGAAGGGA
TAAAAATATTGCTGAAAAAAGGTGTGGAATTTATTGCAAAAAGAGTAAATGATGTTTATGATCTGGGTAGTATAGAGAAT
TATATTGCATCCATAAAGAAAATTATTGGATAA

Upstream 100 bases:

>100_bases
TAGAGGATAGATTAAAAGGAAAATATGTAGTAGATGGAAGGAGAATCCTAAACTATAAAAAGTTAGAGAAAGGTAGATTT
AAGGCAATAGGTGTTAGTTA

Downstream 100 bases:

>100_bases
CAAGTAATTTAGTTTATATCATCCTAAATTATAATAAAAACTTTATATCTAACTACATTTAAATTCTATTTAATAGTATA
AAATAATATTTTAAGATTCA

Product: nucleotidyl transferase

Products: NA

Alternate protein names: Alpha-D-glucosyl-1-phosphate uridylyltransferase; UDP-glucose pyrophosphorylase; UDPGP; Uridine diphosphoglucose pyrophosphorylase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MHAVITAAGLGTRMLPASKEIPKEMFPIPFNNGFKPIIQIIFEQLYNKGIRDFVIVVGRGKRVIEDHFTPDYDFVSYLEK
VGKEKQARELLTFYSKIEKSNIAFVNQPEPKGFGDAVLRVEPFINDKFIVVAADTLLREIPDLIPNSFLVTEVEDPRPYG
VVILEGDRVIDVEEKPKNPKSRFIIVPYYMFTYDIFHALREVKCNGELQLTEGIKILLKKGVEFIAKRVNDVYDLGSIEN
YIASIKKIIG

Sequences:

>Translated_250_residues
MHAVITAAGLGTRMLPASKEIPKEMFPIPFNNGFKPIIQIIFEQLYNKGIRDFVIVVGRGKRVIEDHFTPDYDFVSYLEK
VGKEKQARELLTFYSKIEKSNIAFVNQPEPKGFGDAVLRVEPFINDKFIVVAADTLLREIPDLIPNSFLVTEVEDPRPYG
VVILEGDRVIDVEEKPKNPKSRFIIVPYYMFTYDIFHALREVKCNGELQLTEGIKILLKKGVEFIAKRVNDVYDLGSIEN
YIASIKKIIG
>Mature_250_residues
MHAVITAAGLGTRMLPASKEIPKEMFPIPFNNGFKPIIQIIFEQLYNKGIRDFVIVVGRGKRVIEDHFTPDYDFVSYLEK
VGKEKQARELLTFYSKIEKSNIAFVNQPEPKGFGDAVLRVEPFINDKFIVVAADTLLREIPDLIPNSFLVTEVEDPRPYG
VVILEGDRVIDVEEKPKNPKSRFIIVPYYMFTYDIFHALREVKCNGELQLTEGIKILLKKGVEFIAKRVNDVYDLGSIEN
YIASIKKIIG

Specific function: Lipopolysaccharide biosynthesis. [C]

COG id: COG1210

COG function: function code M; UDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDPGP type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788355, Length=280, Percent_Identity=31.4285714285714, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787488, Length=249, Percent_Identity=32.5301204819277, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788351, Length=270, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790224, Length=245, Percent_Identity=26.9387755102041, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005771
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.9 [H]

Molecular weight: Translated: 28528; Mature: 28528

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAVITAAGLGTRMLPASKEIPKEMFPIPFNNGFKPIIQIIFEQLYNKGIRDFVIVVGRG
CCEEEEECCCCCCCCCCCHHCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
KRVIEDHFTPDYDFVSYLEKVGKEKQARELLTFYSKIEKSNIAFVNQPEPKGFGDAVLRV
CCHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEE
EPFINDKFIVVAADTLLREIPDLIPNSFLVTEVEDPRPYGVVILEGDRVIDVEEKPKNPK
ECCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCEEEECCCCCCCCC
SRFIIVPYYMFTYDIFHALREVKCNGELQLTEGIKILLKKGVEFIAKRVNDVYDLGSIEN
CCEEEEHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
YIASIKKIIG
HHHHHHHHHC
>Mature Secondary Structure
MHAVITAAGLGTRMLPASKEIPKEMFPIPFNNGFKPIIQIIFEQLYNKGIRDFVIVVGRG
CCEEEEECCCCCCCCCCCHHCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
KRVIEDHFTPDYDFVSYLEKVGKEKQARELLTFYSKIEKSNIAFVNQPEPKGFGDAVLRV
CCHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEE
EPFINDKFIVVAADTLLREIPDLIPNSFLVTEVEDPRPYGVVILEGDRVIDVEEKPKNPK
ECCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCEEEECCCCCCCCC
SRFIIVPYYMFTYDIFHALREVKCNGELQLTEGIKILLKKGVEFIAKRVNDVYDLGSIEN
CCEEEEHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
YIASIKKIIG
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]