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The map label for this gene is hemL

Identifier: 227080807

GI number: 227080807

Start: 621714

End: 623012

Strand: Reverse

Name: hemL

Synonym: VCM66_0584

Alternate gene names: 227080807

Gene position: 623012-621714 (Counterclockwise)

Preceding gene: 227080811

Following gene: 227080805

Centisome position: 21.54

GC content: 50.42

Gene sequence:

>1299_bases
ATGACCAAATCAGCCGAGTTGTATCAAAAGGCGCAAACCACGATTCCGGGTGGAGTTAACTCTCCTGTTAGAGCCTTTAA
CGGAGTGGGTGGTTCACCGATTTTCATTGATCGTGCCGATGGCGCGCTGATTTTTGATGCCGATGGTAAAGCATACATTG
ACTACGTGGGATCATGGGGACCAATGATTCTCGGCCACAACCATGCGGTAATTCGTGAAGCGGTGATTCAAGCAGCACAA
CGCGGTTTAAGTTTCGGTGCACCCACCGAGATGGAAATTACCATGGCAGAGTTGGTCTCTGAGCTCGTTCCTTCAATGGA
ACAATTGCGGATGGTGAACTCGGGCACTGAAGCGACGATGAGTGCGATTCGTCTTGCGCGTGGTTATACAGGCCGTGACA
AAATCATCAAGTTTGAAGGCTGTTACCATGGTCATGCCGACAGCTTACTCGTTAAAGCGGGATCTGGGGCTTTGACTCTA
GGCCAACCAAGTTCACCCGGCGTTCCTGCTGATTTTGCCAAGCACACCTTAACTGCTCGTTTTAACGATCTAGACTCAGT
GCGTGAGCTGTTTGCGGCCAATCAAGGTGAAATCGCCTGCATTATCGTTGAACCCGTAGCTGGCAATATGAACTGTATCC
CACCAGTGGAAGGCTTCCACGAAGGGCTGCGTGAAATTTGTGACCAAGAAGGTGCATTACTGATTTTCGATGAAGTGATG
ACTGGTTTTCGTGTCGCATTGGGCGGCGCTCAAGCGCACTACAACATCAAACCCGATTTGACGACACTTGGTAAAGTGAT
CGGTGGCGGTATGCCAGTCGGCGCTTTTGGTGGTCGCCGTGAAGTGATGCAATACATTGCCCCAACAGGCCCGGTTTACC
AAGCTGGTACACTATCAGGCAACCCAATCGCGATGGCAGCGGGCTATGCCTGTCTGAATCTACTGCGTGAAGAAGGCAAT
GAAAAGCGCTTAGCCGCCAAAACCAAGCAACTGGCTGATGGCTTTAAATCTCTGGCCGATCAACATGGTATTCCACTTTT
AGTACACCAAGTTGGCGGCATGTTCGGTTTCTTCTTTACCGAACAAGAGACAGTGACTTGCTATGAAGATGTCGCCCGTT
GCGATGTAGAGCGTTTCAAACGTTTCTTCCATCTGATGCTGCAACACGGCGTTTATCTCGCCCCCTCTGCCTTTGAAGCA
AGCTTTACCTCACTGGCGCACGGTTCAAAAGAGATTGAAGCCACGTTGGAAGCCGCAGATCGTAGCTTTGCGATTTTGGC
AGCGGAAGCGAAAGCGTAA

Upstream 100 bases:

>100_bases
GGAAAATCGATCAAAATGTAGCGAAAACATTTCACCCACTGGAGCATAGGCGTACAATGCGCCCCAAAATTGGTCCGATT
CATCATAAGAGGAAACTAGC

Downstream 100 bases:

>100_bases
CCTACTGTGGGTCACCACTTTGCGAAGGCAGCCTCGGCTGCCTTTTTCATTGAGTGCAGATCTATTGCCAATAATAGAGA
CTCAGTAGCACGAGAGCACA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTKSAELYQKAQTTIPGGVNSPVRAFNGVGGSPIFIDRADGALIFDADGKAYIDYVGSWGPMILGHNHAVIREAVIQAAQ
RGLSFGAPTEMEITMAELVSELVPSMEQLRMVNSGTEATMSAIRLARGYTGRDKIIKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKHTLTARFNDLDSVRELFAANQGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
TGFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRREVMQYIAPTGPVYQAGTLSGNPIAMAAGYACLNLLREEGN
EKRLAAKTKQLADGFKSLADQHGIPLLVHQVGGMFGFFFTEQETVTCYEDVARCDVERFKRFFHLMLQHGVYLAPSAFEA
SFTSLAHGSKEIEATLEAADRSFAILAAEAKA

Sequences:

>Translated_432_residues
MTKSAELYQKAQTTIPGGVNSPVRAFNGVGGSPIFIDRADGALIFDADGKAYIDYVGSWGPMILGHNHAVIREAVIQAAQ
RGLSFGAPTEMEITMAELVSELVPSMEQLRMVNSGTEATMSAIRLARGYTGRDKIIKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKHTLTARFNDLDSVRELFAANQGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
TGFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRREVMQYIAPTGPVYQAGTLSGNPIAMAAGYACLNLLREEGN
EKRLAAKTKQLADGFKSLADQHGIPLLVHQVGGMFGFFFTEQETVTCYEDVARCDVERFKRFFHLMLQHGVYLAPSAFEA
SFTSLAHGSKEIEATLEAADRSFAILAAEAKA
>Mature_431_residues
TKSAELYQKAQTTIPGGVNSPVRAFNGVGGSPIFIDRADGALIFDADGKAYIDYVGSWGPMILGHNHAVIREAVIQAAQR
GLSFGAPTEMEITMAELVSELVPSMEQLRMVNSGTEATMSAIRLARGYTGRDKIIKFEGCYHGHADSLLVKAGSGALTLG
QPSSPGVPADFAKHTLTARFNDLDSVRELFAANQGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVMT
GFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRREVMQYIAPTGPVYQAGTLSGNPIAMAAGYACLNLLREEGNE
KRLAAKTKQLADGFKSLADQHGIPLLVHQVGGMFGFFFTEQETVTCYEDVARCDVERFKRFFHLMLQHGVYLAPSAFEAS
FTSLAHGSKEIEATLEAADRSFAILAAEAKA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=297, Percent_Identity=28.2828282828283, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI37574042, Length=346, Percent_Identity=26.878612716763, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI226442705, Length=347, Percent_Identity=26.2247838616715, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI226442709, Length=313, Percent_Identity=25.8785942492013, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI284507298, Length=211, Percent_Identity=30.3317535545024, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=72.0657276995305, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=362, Percent_Identity=30.1104972375691, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788044, Length=313, Percent_Identity=31.9488817891374, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI145693181, Length=323, Percent_Identity=31.8885448916409, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1787560, Length=378, Percent_Identity=31.2169312169312, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789759, Length=316, Percent_Identity=29.4303797468354, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1786991, Length=334, Percent_Identity=29.0419161676647, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=301, Percent_Identity=27.906976744186, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=359, Percent_Identity=26.4623955431755, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32564660, Length=334, Percent_Identity=26.6467065868263, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=164, Percent_Identity=32.3170731707317, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=321, Percent_Identity=29.2834890965732, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=353, Percent_Identity=24.6458923512748, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356575, Length=349, Percent_Identity=26.3610315186246, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357415, Length=313, Percent_Identity=26.8370607028754, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_VIBC3 (A5F945)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F945
- SMR:   A5F945
- STRING:   A5F945
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0154
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   VCHO345073:VC0395_A0154-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46182; Mature: 46051

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSAELYQKAQTTIPGGVNSPVRAFNGVGGSPIFIDRADGALIFDADGKAYIDYVGSWG
CCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEEECCCCEEEEECCCCEEEEECCCCC
PMILGHNHAVIREAVIQAAQRGLSFGAPTEMEITMAELVSELVPSMEQLRMVNSGTEATM
CEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCHHHHHHHCCCHHHHH
SAIRLARGYTGRDKIIKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKHTLTAR
HHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHH
FNDLDSVRELFAANQGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
HCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHH
TGFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRREVMQYIAPTGPVYQAGTLSG
HHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGYACLNLLREEGNEKRLAAKTKQLADGFKSLADQHGIPLLVHQVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHCCHHEEEEC
EQETVTCYEDVARCDVERFKRFFHLMLQHGVYLAPSAFEASFTSLAHGSKEIEATLEAAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCHHHHHHHHHCC
RSFAILAAEAKA
CCEEEEEECCCC
>Mature Secondary Structure 
TKSAELYQKAQTTIPGGVNSPVRAFNGVGGSPIFIDRADGALIFDADGKAYIDYVGSWG
CCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEEECCCCEEEEECCCCEEEEECCCCC
PMILGHNHAVIREAVIQAAQRGLSFGAPTEMEITMAELVSELVPSMEQLRMVNSGTEATM
CEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCHHHHHHHCCCHHHHH
SAIRLARGYTGRDKIIKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKHTLTAR
HHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHH
FNDLDSVRELFAANQGEIACIIVEPVAGNMNCIPPVEGFHEGLREICDQEGALLIFDEVM
HCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHH
TGFRVALGGAQAHYNIKPDLTTLGKVIGGGMPVGAFGGRREVMQYIAPTGPVYQAGTLSG
HHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMAAGYACLNLLREEGNEKRLAAKTKQLADGFKSLADQHGIPLLVHQVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHCCHHEEEEC
EQETVTCYEDVARCDVERFKRFFHLMLQHGVYLAPSAFEASFTSLAHGSKEIEATLEAAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCHHHHHHHHHCC
RSFAILAAEAKA
CCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA