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The map label for this gene is htrA [H]

Identifier: 227080749

GI number: 227080749

Start: 557085

End: 558455

Strand: Reverse

Name: htrA [H]

Synonym: VCM66_0524

Alternate gene names: 227080749

Gene position: 558455-557085 (Counterclockwise)

Preceding gene: 227080750

Following gene: 227080748

Centisome position: 19.31

GC content: 47.92

Gene sequence:

>1371_bases
ATGATGAAAAAACCTTTACTTGTTTTAACTGCTCTGTCTCTTAGTTTGAGCGCGATTCTCTCGCCTTTGCCTGCAACTGC
AGCGCTTCCTCTCTCAGTCAATGGAGAGCAGATTCCTAGCCTAGCCCCCATGCTTGAAAAAGTCACACCCGCCGTGGTGA
GCATTGCTGTGGAAGGGACTCAAGTTTCAAGACAGCGTCTGCCGGATCAGTTTCGTTTTTTCTTCGGACCGGATTTTCCG
ACCGAACAACTCCAAGAGCGACCTTTCCGTGGCTTAGGTTCTGGGGTCATCATTAACGCTGATAAAGGGTATGTCGTCAC
TAACTACCATGTCATTAATGGTGCTGAAAAAATTCGCGTCAAACTGTATGACGGTCGCGAGTTTGATGCAGAACTTGTCG
GTGGTGATGAGATGTCTGATGTCGCCTTGCTCAAGCTAAACAAAGCGAAAAACCTCACTGAGATCCGTATCGCGGACTCC
GATAAACTGCGAGTCGGTGATTTTGCAGTGGCCATCGGTAACCCATTTGGCTTAGGGCAAACTGTGACCTCTGGCATTGT
CTCAGCCTTAGGGCGTAGTGGTTTGAATATCGAAAACTTTGAAAACTTCATCCAGACCGATGCCGCCATCAACAGCGGCA
ACTCAGGAGGAGCTCTGGTTAACCTTAATGGTGAACTCATCGGTATCAACACCGCGATCCTTGGTCCAAACGGTGGCAAC
GTCGGTATAGGTTTTGCCATCCCATCGAATATGATGAAAAATCTGACCGATCAAATTCTTGAGTTTGGTGAAGTGAAACG
CGGCATGCTGGGTGTACAAGGCGGTGAAATCACTTCCGAACTGGCTGATGCGCTCGGCTATGAATCCTCAAAAGGTGCTT
TTGTCAGCCAAGTGGTTCCTGACAGTGCTGCGGACAAAGCGGGCATCAAAGCGGGTGACATCATTACGTCGCTGAATGGT
AAAAAAATCGATACCTTCTCTGAGCTACGCGCGAAAGTCGCGACCCTAGGCGCAGGAAAAACCATTACCCTTGGAGTGCT
GCGTGATGGTAAGAATCAAAATATTGATGTAACGCTTGGGGAGCAGCAAAATGCCAAGACCAAAGCAGAATCACTGCATC
AAGGTTTGAGCGGCGCGGAGTTAAGCAACACCACTGACAGCGATCCTATTCAGGGCGTTAAGGTTACTGAGGTTCAAAAA
GGCTCTGCCGCTGAATCTTACCAGCTACAAAAAGACGACATTATCATTGGCGTTAACCGTAAGCGGGTGAAAAATATCGC
CGAGTTGCGTGCGATTATGGAAAAATCACCGAATATTTTGGCATTAAATATCCAACGTGGAGAGAGAACGCTTTACTTGG
TTGTTCGTTAA

Upstream 100 bases:

>100_bases
TGTAAAGGTTTTTGAGTCATAACTCGCAGACTGTCAGTTGAAGTTTCTTGAAATGGACATATTTTCTACTTCAACCGGAC
GTTAATTTGTTGAGGAGCTT

Downstream 100 bases:

>100_bases
TTATCACTCAGCGTTACCGCTCATTGAGCGGTAACGCTTTCTTTCCCTCAAAAATCCCCAAACTTGCACAAAAACTTATT
CATAAGCTACAGATGCAACT

Product: protease DO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MMKKPLLVLTALSLSLSAILSPLPATAALPLSVNGEQIPSLAPMLEKVTPAVVSIAVEGTQVSRQRLPDQFRFFFGPDFP
TEQLQERPFRGLGSGVIINADKGYVVTNYHVINGAEKIRVKLYDGREFDAELVGGDEMSDVALLKLNKAKNLTEIRIADS
DKLRVGDFAVAIGNPFGLGQTVTSGIVSALGRSGLNIENFENFIQTDAAINSGNSGGALVNLNGELIGINTAILGPNGGN
VGIGFAIPSNMMKNLTDQILEFGEVKRGMLGVQGGEITSELADALGYESSKGAFVSQVVPDSAADKAGIKAGDIITSLNG
KKIDTFSELRAKVATLGAGKTITLGVLRDGKNQNIDVTLGEQQNAKTKAESLHQGLSGAELSNTTDSDPIQGVKVTEVQK
GSAAESYQLQKDDIIIGVNRKRVKNIAELRAIMEKSPNILALNIQRGERTLYLVVR

Sequences:

>Translated_456_residues
MMKKPLLVLTALSLSLSAILSPLPATAALPLSVNGEQIPSLAPMLEKVTPAVVSIAVEGTQVSRQRLPDQFRFFFGPDFP
TEQLQERPFRGLGSGVIINADKGYVVTNYHVINGAEKIRVKLYDGREFDAELVGGDEMSDVALLKLNKAKNLTEIRIADS
DKLRVGDFAVAIGNPFGLGQTVTSGIVSALGRSGLNIENFENFIQTDAAINSGNSGGALVNLNGELIGINTAILGPNGGN
VGIGFAIPSNMMKNLTDQILEFGEVKRGMLGVQGGEITSELADALGYESSKGAFVSQVVPDSAADKAGIKAGDIITSLNG
KKIDTFSELRAKVATLGAGKTITLGVLRDGKNQNIDVTLGEQQNAKTKAESLHQGLSGAELSNTTDSDPIQGVKVTEVQK
GSAAESYQLQKDDIIIGVNRKRVKNIAELRAIMEKSPNILALNIQRGERTLYLVVR
>Mature_456_residues
MMKKPLLVLTALSLSLSAILSPLPATAALPLSVNGEQIPSLAPMLEKVTPAVVSIAVEGTQVSRQRLPDQFRFFFGPDFP
TEQLQERPFRGLGSGVIINADKGYVVTNYHVINGAEKIRVKLYDGREFDAELVGGDEMSDVALLKLNKAKNLTEIRIADS
DKLRVGDFAVAIGNPFGLGQTVTSGIVSALGRSGLNIENFENFIQTDAAINSGNSGGALVNLNGELIGINTAILGPNGGN
VGIGFAIPSNMMKNLTDQILEFGEVKRGMLGVQGGEITSELADALGYESSKGAFVSQVVPDSAADKAGIKAGDIITSLNG
KKIDTFSELRAKVATLGAGKTITLGVLRDGKNQNIDVTLGEQQNAKTKAESLHQGLSGAELSNTTDSDPIQGVKVTEVQK
GSAAESYQLQKDDIIIGVNRKRVKNIAELRAIMEKSPNILALNIQRGERTLYLVVR

Specific function: Protease with a shared specificity with degP [H]

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PDZ (DHR) domains [H]

Homologues:

Organism=Homo sapiens, GI22129776, Length=312, Percent_Identity=36.8589743589744, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4506141, Length=297, Percent_Identity=37.037037037037, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI24308541, Length=327, Percent_Identity=31.8042813455657, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI7019477, Length=315, Percent_Identity=33.968253968254, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1789629, Length=457, Percent_Identity=58.2056892778993, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1786356, Length=457, Percent_Identity=58.6433260393873, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI1789630, Length=276, Percent_Identity=45.6521739130435, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24646839, Length=288, Percent_Identity=34.7222222222222, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011782
- InterPro:   IPR001254
- InterPro:   IPR001940 [H]

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin [H]

EC number: 3.4.21.-

Molecular weight: Translated: 48367; Mature: 48367

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50106 PDZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKPLLVLTALSLSLSAILSPLPATAALPLSVNGEQIPSLAPMLEKVTPAVVSIAVEGT
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHCCHHHHHHHHHCCEEEEEEECCC
QVSRQRLPDQFRFFFGPDFPTEQLQERPFRGLGSGVIINADKGYVVTNYHVINGAEKIRV
HHHHHHCCCHHEEEECCCCCHHHHHHCCCCCCCCCEEEECCCCEEEEEEEEECCCEEEEE
KLYDGREFDAELVGGDEMSDVALLKLNKAKNLTEIRIADSDKLRVGDFAVAIGNPFGLGQ
EEECCCCCCCEEECCCCCCCEEEEEECCCCCCEEEEECCCCCEEECEEEEEECCCCCCCH
TVTSGIVSALGRSGLNIENFENFIQTDAAINSGNSGGALVNLNGELIGINTAILGPNGGN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEEEEECCCCCC
VGIGFAIPSNMMKNLTDQILEFGEVKRGMLGVQGGEITSELADALGYESSKGAFVSQVVP
EEEEEECCHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHCCCCCCCCEEEHHCC
DSAADKAGIKAGDIITSLNGKKIDTFSELRAKVATLGAGKTITLGVLRDGKNQNIDVTLG
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEC
EQQNAKTKAESLHQGLSGAELSNTTDSDPIQGVKVTEVQKGSAAESYQLQKDDIIIGVNR
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCCCEEEECCH
KRVKNIAELRAIMEKSPNILALNIQRGERTLYLVVR
HHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEC
>Mature Secondary Structure
MMKKPLLVLTALSLSLSAILSPLPATAALPLSVNGEQIPSLAPMLEKVTPAVVSIAVEGT
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHCCHHHHHHHHHCCEEEEEEECCC
QVSRQRLPDQFRFFFGPDFPTEQLQERPFRGLGSGVIINADKGYVVTNYHVINGAEKIRV
HHHHHHCCCHHEEEECCCCCHHHHHHCCCCCCCCCEEEECCCCEEEEEEEEECCCEEEEE
KLYDGREFDAELVGGDEMSDVALLKLNKAKNLTEIRIADSDKLRVGDFAVAIGNPFGLGQ
EEECCCCCCCEEECCCCCCCEEEEEECCCCCCEEEEECCCCCEEECEEEEEECCCCCCCH
TVTSGIVSALGRSGLNIENFENFIQTDAAINSGNSGGALVNLNGELIGINTAILGPNGGN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEEEEECCCCCC
VGIGFAIPSNMMKNLTDQILEFGEVKRGMLGVQGGEITSELADALGYESSKGAFVSQVVP
EEEEEECCHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHCCCCCCCCEEEHHCC
DSAADKAGIKAGDIITSLNGKKIDTFSELRAKVATLGAGKTITLGVLRDGKNQNIDVTLG
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEC
EQQNAKTKAESLHQGLSGAELSNTTDSDPIQGVKVTEVQKGSAAESYQLQKDDIIIGVNR
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCCCEEEECCH
KRVKNIAELRAIMEKSPNILALNIQRGERTLYLVVR
HHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8576051; 9278503 [H]