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The map label for this gene is degS [H]

Identifier: 227080748

GI number: 227080748

Start: 555850

End: 556908

Strand: Reverse

Name: degS [H]

Synonym: VCM66_0523

Alternate gene names: 227080748

Gene position: 556908-555850 (Counterclockwise)

Preceding gene: 227080749

Following gene: 227080742

Centisome position: 19.25

GC content: 49.01

Gene sequence:

>1059_bases
ATGCTGAAATTTTGGGTTCGCTCAATCAGCCTTGGGTTGTTGGCTGCGATTGCCATTATTATGGTGACACCCTCACTACG
CGCCAAATTAATGCCCGTTGTCGAACAACCACGCAACATCGGCGCTCTACAAATCTCATTTAATGAAGCGGTACGCAAAG
CCGCCCCTGCCGTCGTCAATATTTATAACCGTAAATACAGCGAAAATGATCGCCGTAAACTCTCGATTCAAGGTTTAGGA
TCCGGTGTCATTGTCAGCGAAAAAGGCTACATCATCACCAACTACCACGTCGTCGCGCAGGCCGATCAAATTGTCGTTGC
TCTACAAGATGGGCGAGCCGCAGCAGCACAATTGGTGGGAAAAGATCGCCGTACCGATATTGCCGTATTACGCGTAGAAG
GCACGGGTTTACCAGTGATTCCACTCAATCCAGATTACCATCCTAAAGTGGGGGACGTGGTGTTGGCGATTGGTAACCCT
TACAACTTAGGGCAAACCACGACTTTCGGAATTATCTCGGCTACCGGACGTTCATCCATCAGCGCTGATGGTCGCCAAGC
CTTTATTCAAACTGATGCCGCAATCAATGACGGCAACTCAGGTGGTGCATTGGTCAATACCCAAGGTGAACTGGTCGGCA
TCAATACCGCCTCTTTTCAACAAGCCACCGATCTCGAAACTTACGGGATTTCGTTTGCGATTCCCTACTCTTTGGCCAGT
AAAATTATGACCAAAATCATTGCTGATGGCCGCGTGATCCGCGGTTATATTGGCGTCGACGGTCAAGATATTAACTCGAT
GACATCACGTTTGCTGGGGAATGAGCATGTCGGTGGGATCATTATTTTAGGGGTTGACCCGAATGGACCCGCAGCCCGAG
CAGGCTTTCTGGAGCAAGATATTTTGCTGAAAATCGACGGTAAAAAAATTAATGGTCGCCAGAATGTCACAGATACCGTC
ACCGATCTTCGCCCCGGCACTGTGGTGGATTTCACCCTACTGCGTAAGGGTGAAGAGATTGTACTCCCAGTTACGATTGG
TGAAGACACTCGTGATTAG

Upstream 100 bases:

>100_bases
TATTCATAAGCTACAGATGCAACTTTGGCGAAATGCCTTTCCAGTGTTATTCTTGCCCACTCGCAGTGAGGAATTTAGTC
AATATTGAGTTGGGGAAACT

Downstream 100 bases:

>100_bases
ACGAGTGTGATTCTGAAAGGTTCCACTCATCGACGATGAGACCTCGACGTTAAAAAATGGAGCACAAAAAAGCGGAGCCC
TTGGGCTCCGCTTTTTACTA

Product: protease DegS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MLKFWVRSISLGLLAAIAIIMVTPSLRAKLMPVVEQPRNIGALQISFNEAVRKAAPAVVNIYNRKYSENDRRKLSIQGLG
SGVIVSEKGYIITNYHVVAQADQIVVALQDGRAAAAQLVGKDRRTDIAVLRVEGTGLPVIPLNPDYHPKVGDVVLAIGNP
YNLGQTTTFGIISATGRSSISADGRQAFIQTDAAINDGNSGGALVNTQGELVGINTASFQQATDLETYGISFAIPYSLAS
KIMTKIIADGRVIRGYIGVDGQDINSMTSRLLGNEHVGGIIILGVDPNGPAARAGFLEQDILLKIDGKKINGRQNVTDTV
TDLRPGTVVDFTLLRKGEEIVLPVTIGEDTRD

Sequences:

>Translated_352_residues
MLKFWVRSISLGLLAAIAIIMVTPSLRAKLMPVVEQPRNIGALQISFNEAVRKAAPAVVNIYNRKYSENDRRKLSIQGLG
SGVIVSEKGYIITNYHVVAQADQIVVALQDGRAAAAQLVGKDRRTDIAVLRVEGTGLPVIPLNPDYHPKVGDVVLAIGNP
YNLGQTTTFGIISATGRSSISADGRQAFIQTDAAINDGNSGGALVNTQGELVGINTASFQQATDLETYGISFAIPYSLAS
KIMTKIIADGRVIRGYIGVDGQDINSMTSRLLGNEHVGGIIILGVDPNGPAARAGFLEQDILLKIDGKKINGRQNVTDTV
TDLRPGTVVDFTLLRKGEEIVLPVTIGEDTRD
>Mature_352_residues
MLKFWVRSISLGLLAAIAIIMVTPSLRAKLMPVVEQPRNIGALQISFNEAVRKAAPAVVNIYNRKYSENDRRKLSIQGLG
SGVIVSEKGYIITNYHVVAQADQIVVALQDGRAAAAQLVGKDRRTDIAVLRVEGTGLPVIPLNPDYHPKVGDVVLAIGNP
YNLGQTTTFGIISATGRSSISADGRQAFIQTDAAINDGNSGGALVNTQGELVGINTASFQQATDLETYGISFAIPYSLAS
KIMTKIIADGRVIRGYIGVDGQDINSMTSRLLGNEHVGGIIILGVDPNGPAARAGFLEQDILLKIDGKKINGRQNVTDTV
TDLRPGTVVDFTLLRKGEEIVLPVTIGEDTRD

Specific function: Senses the OMP signal triggered by the accumulation of unassembled porins in the envelope and then initiates rseA degradation by cleaving it in its periplasmic domain, making it an attractive substrate for subsequent cleavage by rseP [H]

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Homo sapiens, GI4506141, Length=326, Percent_Identity=34.6625766871166, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI22129776, Length=311, Percent_Identity=34.4051446945338, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI24308541, Length=325, Percent_Identity=31.0769230769231, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI7019477, Length=317, Percent_Identity=32.1766561514196, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1789630, Length=351, Percent_Identity=51.2820512820513, Blast_Score=329, Evalue=1e-91,
Organism=Escherichia coli, GI1789629, Length=355, Percent_Identity=35.7746478873239, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786356, Length=276, Percent_Identity=40.5797101449275, Blast_Score=192, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24646839, Length=374, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011783
- InterPro:   IPR001254
- InterPro:   IPR001940 [H]

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin [H]

EC number: 3.4.21.-

Molecular weight: Translated: 37556; Mature: 37556

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFWVRSISLGLLAAIAIIMVTPSLRAKLMPVVEQPRNIGALQISFNEAVRKAAPAVVN
CCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCEEE
IYNRKYSENDRRKLSIQGLGSGVIVSEKGYIITNYHVVAQADQIVVALQDGRAAAAQLVG
EECCCCCCCCCEEEEEEECCCEEEEECCCEEEEEEEEEEECCEEEEEEECCCHHHHHHHC
KDRRTDIAVLRVEGTGLPVIPLNPDYHPKVGDVVLAIGNPYNLGQTTTFGIISATGRSSI
CCCCCEEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCC
SADGRQAFIQTDAAINDGNSGGALVNTQGELVGINTASFQQATDLETYGISFAIPYSLAS
CCCCCEEEEEECCEECCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEHHHHHH
KIMTKIIADGRVIRGYIGVDGQDINSMTSRLLGNEHVGGIIILGVDPNGPAARAGFLEQD
HHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHCCCCCCC
ILLKIDGKKINGRQNVTDTVTDLRPGTVVDFTLLRKGEEIVLPVTIGEDTRD
EEEEECCEEECCCCCCCHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MLKFWVRSISLGLLAAIAIIMVTPSLRAKLMPVVEQPRNIGALQISFNEAVRKAAPAVVN
CCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCEEE
IYNRKYSENDRRKLSIQGLGSGVIVSEKGYIITNYHVVAQADQIVVALQDGRAAAAQLVG
EECCCCCCCCCEEEEEEECCCEEEEECCCEEEEEEEEEEECCEEEEEEECCCHHHHHHHC
KDRRTDIAVLRVEGTGLPVIPLNPDYHPKVGDVVLAIGNPYNLGQTTTFGIISATGRSSI
CCCCCEEEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCC
SADGRQAFIQTDAAINDGNSGGALVNTQGELVGINTASFQQATDLETYGISFAIPYSLAS
CCCCCEEEEEECCEECCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEHHHHHH
KIMTKIIADGRVIRGYIGVDGQDINSMTSRLLGNEHVGGIIILGVDPNGPAARAGFLEQD
HHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHCCCCCCC
ILLKIDGKKINGRQNVTDTVTDLRPGTVVDFTLLRKGEEIVLPVTIGEDTRD
EEEEECCEEECCCCCCCHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]