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The map label for this gene is lysC [H]

Identifier: 227080602

GI number: 227080602

Start: 397517

End: 398956

Strand: Reverse

Name: lysC [H]

Synonym: VCM66_0376

Alternate gene names: 227080602

Gene position: 398956-397517 (Counterclockwise)

Preceding gene: 227080604

Following gene: 227080600

Centisome position: 13.79

GC content: 50.07

Gene sequence:

>1440_bases
TTGCTCTATAAAAAACACTCTCTTCACAACATTGGACGTTGGGTTACCAACAACTCGTTTTTCAGGAAGTCTGGGAGAAT
TGCCGTGAGCACATTTAACGTCGCCAAATTTGGTGGTACTAGTGTCGCCAACTTTGAAGCCATGAGCCGTTGTTCGGCCA
TTATCGAAAACAATCCACAAACACGCTTAGTGGTCAGCAGCGCCTGCTCTGGGGTGACCAATTTATTGGTCGAACTCGCC
AATGGCGTGGCCTCAACTGAACGCCGCCAAGCCATTTTGCAACAGTTGGCCGATATCCATTATGTGATTATCGACCAGCT
CGCCGATCCGACACGCGTCGAAAAAGAAGTCAAAGCCGTGCTAGATTCTGTGGCCAGTGCTGCAGAAGCGGCGTCATTTC
AATCCAGTAAAAAACTGACCGACCATCTCGTCGCCTGTGGTGAGCTCATCTCCACCTACATCCTCACTCAACTGATGCGT
GAGCGTGGTGTCGACGCGGTACGTTTTGATATCCGCGAAGTGCTACGCACTGACGATCATTACGGTCGCGCCGAACCTCA
ATTGAAAGAAATCGCTCAATTAGCCAAAGAAAAACTGGTGCCACTGTGTGAGCAATATGTCGTCGTGACTCAAGGTTTTA
TCGGCTCTGATGCGCAAGGTAATACCACCACTTTAGGCCGCGGCGGCAGTGATTATTCAGCCGCTTTGATTGCCGAAGCC
GTGCAAGCAAGTGGTTTAGAGATTTGGACTGACGTACCGGGAATCTACAGTACCGATCCACGTATCGCGCCAAAAGCGGC
GCCGATTCCTGAGATAAGCTTTAGCGAAGCCTCAGAAATGGCCAATTTTGGTGCCAAGATTTTGCATCCATCCACCTTGC
TTCCAGCGGTACGCCATGGCATTCCCGTGTTTGTTGGCTCATCAAAAGAACCGGAAAAAGGTGGCACTTGGATCCGTAAA
CAAGTCGAAAGCGCGCCACTGTATCGCGCACTTGCACTACGCTGTAATCAAACCATGGTGACACTGCGTAGCGCGCAGAT
GTTCCATGCTTACGGTTTCCTCGCCAAAGTGTTCGAAATTCTCGCCAAGCATAAAATTTCGGTCGATCTCATCACCACAT
CAGAAATCAGCGTGTCACTGACACTGGATAAAACCGATACCTCAGGCGGTGCGCCAGAATTGCCTGAAGCGGTGCGCGCC
GAGTTAGAGGAGCTATGTACGGTCGAAGTCGAATACAACCTGTGTCTGATTGCTCTGATCGGCAACAAGATGAAAGAGAG
CCGCGGCTACGCGAAGCAAGTGTTCAGTGTATTGGAAGATTACAACCTCCGTATGATTTGCTATGGCGCCAGCGCGCATA
ACCTCTGTTTCTTGCTGCATGAATCTGTCTCTAAACAAGCGGTGCAGAAGCTGCATAAAGAGCTGTTTGAGCAAGAGTAA

Upstream 100 bases:

>100_bases
TTGGTTTATCGGTTGAACGGGCTGAATCCCTTCAACTGTCATCAGCTCGAATCTGATGAAGAGCTTCTGAGGGAAATCTT
TCTATAGTCACTCCTCTATT

Downstream 100 bases:

>100_bases
AATCAAACTCACGAGATAAAACCAGAGGCCACATGATGTGGCCTCTTTTATTGTGGATGGAGGTCTTACTGATTAAGCAT
CAAGGTTAGGCGCTAGCCAC

Product: aspartate kinase III

Products: NA

Alternate protein names: Aspartate kinase III; AKIII; Lysine-sensitive aspartokinase III [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MLYKKHSLHNIGRWVTNNSFFRKSGRIAVSTFNVAKFGGTSVANFEAMSRCSAIIENNPQTRLVVSSACSGVTNLLVELA
NGVASTERRQAILQQLADIHYVIIDQLADPTRVEKEVKAVLDSVASAAEAASFQSSKKLTDHLVACGELISTYILTQLMR
ERGVDAVRFDIREVLRTDDHYGRAEPQLKEIAQLAKEKLVPLCEQYVVVTQGFIGSDAQGNTTTLGRGGSDYSAALIAEA
VQASGLEIWTDVPGIYSTDPRIAPKAAPIPEISFSEASEMANFGAKILHPSTLLPAVRHGIPVFVGSSKEPEKGGTWIRK
QVESAPLYRALALRCNQTMVTLRSAQMFHAYGFLAKVFEILAKHKISVDLITTSEISVSLTLDKTDTSGGAPELPEAVRA
ELEELCTVEVEYNLCLIALIGNKMKESRGYAKQVFSVLEDYNLRMICYGASAHNLCFLLHESVSKQAVQKLHKELFEQE

Sequences:

>Translated_479_residues
MLYKKHSLHNIGRWVTNNSFFRKSGRIAVSTFNVAKFGGTSVANFEAMSRCSAIIENNPQTRLVVSSACSGVTNLLVELA
NGVASTERRQAILQQLADIHYVIIDQLADPTRVEKEVKAVLDSVASAAEAASFQSSKKLTDHLVACGELISTYILTQLMR
ERGVDAVRFDIREVLRTDDHYGRAEPQLKEIAQLAKEKLVPLCEQYVVVTQGFIGSDAQGNTTTLGRGGSDYSAALIAEA
VQASGLEIWTDVPGIYSTDPRIAPKAAPIPEISFSEASEMANFGAKILHPSTLLPAVRHGIPVFVGSSKEPEKGGTWIRK
QVESAPLYRALALRCNQTMVTLRSAQMFHAYGFLAKVFEILAKHKISVDLITTSEISVSLTLDKTDTSGGAPELPEAVRA
ELEELCTVEVEYNLCLIALIGNKMKESRGYAKQVFSVLEDYNLRMICYGASAHNLCFLLHESVSKQAVQKLHKELFEQE
>Mature_479_residues
MLYKKHSLHNIGRWVTNNSFFRKSGRIAVSTFNVAKFGGTSVANFEAMSRCSAIIENNPQTRLVVSSACSGVTNLLVELA
NGVASTERRQAILQQLADIHYVIIDQLADPTRVEKEVKAVLDSVASAAEAASFQSSKKLTDHLVACGELISTYILTQLMR
ERGVDAVRFDIREVLRTDDHYGRAEPQLKEIAQLAKEKLVPLCEQYVVVTQGFIGSDAQGNTTTLGRGGSDYSAALIAEA
VQASGLEIWTDVPGIYSTDPRIAPKAAPIPEISFSEASEMANFGAKILHPSTLLPAVRHGIPVFVGSSKEPEKGGTWIRK
QVESAPLYRALALRCNQTMVTLRSAQMFHAYGFLAKVFEILAKHKISVDLITTSEISVSLTLDKTDTSGGAPELPEAVRA
ELEELCTVEVEYNLCLIALIGNKMKESRGYAKQVFSVLEDYNLRMICYGASAHNLCFLLHESVSKQAVQKLHKELFEQE

Specific function: Lysine biosynthesis; first step. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1790455, Length=450, Percent_Identity=54.4444444444444, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786183, Length=471, Percent_Identity=29.723991507431, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI1790376, Length=466, Percent_Identity=26.1802575107296, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320893, Length=502, Percent_Identity=30.2788844621514, Blast_Score=184, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 52525; Mature: 52525

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYKKHSLHNIGRWVTNNSFFRKSGRIAVSTFNVAKFGGTSVANFEAMSRCSAIIENNPQ
CCCCCCCHHHHHHHHCCCCHHHCCCCEEEEEECCHHCCCCCHHHHHHHHHHHHHHCCCCC
TRLVVSSACSGVTNLLVELANGVASTERRQAILQQLADIHYVIIDQLADPTRVEKEVKAV
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LDSVASAAEAASFQSSKKLTDHLVACGELISTYILTQLMRERGVDAVRFDIREVLRTDDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
YGRAEPQLKEIAQLAKEKLVPLCEQYVVVTQGFIGSDAQGNTTTLGRGGSDYSAALIAEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHH
VQASGLEIWTDVPGIYSTDPRIAPKAAPIPEISFSEASEMANFGAKILHPSTLLPAVRHG
HHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHCC
IPVFVGSSKEPEKGGTWIRKQVESAPLYRALALRCNQTMVTLRSAQMFHAYGFLAKVFEI
CEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LAKHKISVDLITTSEISVSLTLDKTDTSGGAPELPEAVRAELEELCTVEVEYNLCLIALI
HHHCCCEEEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHEEEEECCEEEEEEC
GNKMKESRGYAKQVFSVLEDYNLRMICYGASAHNLCFLLHESVSKQAVQKLHKELFEQE
CCHHHHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLYKKHSLHNIGRWVTNNSFFRKSGRIAVSTFNVAKFGGTSVANFEAMSRCSAIIENNPQ
CCCCCCCHHHHHHHHCCCCHHHCCCCEEEEEECCHHCCCCCHHHHHHHHHHHHHHCCCCC
TRLVVSSACSGVTNLLVELANGVASTERRQAILQQLADIHYVIIDQLADPTRVEKEVKAV
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LDSVASAAEAASFQSSKKLTDHLVACGELISTYILTQLMRERGVDAVRFDIREVLRTDDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
YGRAEPQLKEIAQLAKEKLVPLCEQYVVVTQGFIGSDAQGNTTTLGRGGSDYSAALIAEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHH
VQASGLEIWTDVPGIYSTDPRIAPKAAPIPEISFSEASEMANFGAKILHPSTLLPAVRHG
HHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHCC
IPVFVGSSKEPEKGGTWIRKQVESAPLYRALALRCNQTMVTLRSAQMFHAYGFLAKVFEI
CEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LAKHKISVDLITTSEISVSLTLDKTDTSGGAPELPEAVRAELEELCTVEVEYNLCLIALI
HHHCCCEEEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHEEEEECCEEEEEEC
GNKMKESRGYAKQVFSVLEDYNLRMICYGASAHNLCFLLHESVSKQAVQKLHKELFEQE
CCHHHHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3003049; 8265357; 9278503; 6312411 [H]