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The map label for this gene is ghrA [H]

Identifier: 227080482

GI number: 227080482

Start: 257289

End: 258212

Strand: Reverse

Name: ghrA [H]

Synonym: VCM66_0252

Alternate gene names: 227080482

Gene position: 258212-257289 (Counterclockwise)

Preceding gene: 227080486

Following gene: 227080481

Centisome position: 8.93

GC content: 53.25

Gene sequence:

>924_bases
ATGAACAATTTCCACCAGCTGTATATTCTGACTGAACATGATGAACACTACCGCGAGCAGATCCTTGAGCGCCAACTGGA
AGGGCTCGCCTTGACCGAAGATCGCGCCAAGGCCACGATTTTGTTGGCCGCACCGCCGATGGCCGCCCGCCGTTTGGATG
AGTTTCCGCGCTTGCAATGGCTGCATTCCGCCTATGCCGGCGTCGATACCTTGATGGACCCCAAACTGCGCAAAAACTAT
CTGCTGACCAACGTCAAAGGCATCTTCGGGCACCTGATCGCCGAGTATGTGATGGGTTACGCTATCCAATATCAGCGCGA
TTTTCGCCTGTATCAAACCCAACAAGCTGAGCGCCTTTGGCAGCCGCGCCCTTACTCTTCACTTGCCAATCAAACCCTAG
TGATTTTGGGCACTGGCTCGATTGGTAGCCATTTAGCCCATGTGGCTAAACAGTTTGGGCTGCGAGTGGTGGGCGTCAAT
CGCACCGGCATTCCGGCCAAAGAAGGGCATTTTGATGCCACTTATCACATCAGTGAGCTGCCAGCCGCTTTAATGCGCGC
CGACTTACTGGTGAATACGCTGCCCAACACCCCCGCAACCGAAGGGCTGCTCAATCAAGAAAATCTTCGCCACTGCCACC
AAGCGCTGCTGTTTAACGTCGGGCGTGGCAAAACGTTAGTAGAGCAGGGTCTGCCGGATTTAATTGCAGCAGGACACATT
CGCCACGCGTTTTTGGATGTGTTTATCAAAGAGCCACTCGCTCAAGATCACCCATTCTGGGACAATCCGGCGATCACCAT
CACACCACACATCGCGGCGGTCAGCTTTCCAGAGCAAGTGGTCGATATCTTCGCCGATAACTACCAACGTTGGTGCGATA
ACTTGCCACTGCGTAATCAGATTGATTTTGAAAAAGGCTACTGA

Upstream 100 bases:

>100_bases
ACGAAGCTGTAGAGGGTTTTGTAACATAGTCACTTCAAATCGTTGCGGAAAAAGACAGCGGAAGTGTACTGGATAACCAT
AAGGGAAAAAAGACGCGCCT

Downstream 100 bases:

>100_bases
TGCTTGAGTCACTGCTGACGGAAATTCGCCAGTGCACAGTGTGTGAACCCCATTTACCCTTGGGCGCGAATCCGGTGATC
CGCGCCCATCCGGCGGCGAA

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNNFHQLYILTEHDEHYREQILERQLEGLALTEDRAKATILLAAPPMAARRLDEFPRLQWLHSAYAGVDTLMDPKLRKNY
LLTNVKGIFGHLIAEYVMGYAIQYQRDFRLYQTQQAERLWQPRPYSSLANQTLVILGTGSIGSHLAHVAKQFGLRVVGVN
RTGIPAKEGHFDATYHISELPAALMRADLLVNTLPNTPATEGLLNQENLRHCHQALLFNVGRGKTLVEQGLPDLIAAGHI
RHAFLDVFIKEPLAQDHPFWDNPAITITPHIAAVSFPEQVVDIFADNYQRWCDNLPLRNQIDFEKGY

Sequences:

>Translated_307_residues
MNNFHQLYILTEHDEHYREQILERQLEGLALTEDRAKATILLAAPPMAARRLDEFPRLQWLHSAYAGVDTLMDPKLRKNY
LLTNVKGIFGHLIAEYVMGYAIQYQRDFRLYQTQQAERLWQPRPYSSLANQTLVILGTGSIGSHLAHVAKQFGLRVVGVN
RTGIPAKEGHFDATYHISELPAALMRADLLVNTLPNTPATEGLLNQENLRHCHQALLFNVGRGKTLVEQGLPDLIAAGHI
RHAFLDVFIKEPLAQDHPFWDNPAITITPHIAAVSFPEQVVDIFADNYQRWCDNLPLRNQIDFEKGY
>Mature_307_residues
MNNFHQLYILTEHDEHYREQILERQLEGLALTEDRAKATILLAAPPMAARRLDEFPRLQWLHSAYAGVDTLMDPKLRKNY
LLTNVKGIFGHLIAEYVMGYAIQYQRDFRLYQTQQAERLWQPRPYSSLANQTLVILGTGSIGSHLAHVAKQFGLRVVGVN
RTGIPAKEGHFDATYHISELPAALMRADLLVNTLPNTPATEGLLNQENLRHCHQALLFNVGRGKTLVEQGLPDLIAAGHI
RHAFLDVFIKEPLAQDHPFWDNPAITITPHIAAVSFPEQVVDIFADNYQRWCDNLPLRNQIDFEKGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=293, Percent_Identity=24.5733788395904, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI6912396, Length=151, Percent_Identity=31.7880794701987, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI87081824, Length=252, Percent_Identity=31.7460317460317, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI87082289, Length=239, Percent_Identity=26.3598326359833, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=241, Percent_Identity=26.9709543568465, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321253, Length=149, Percent_Identity=32.8859060402685, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325144, Length=157, Percent_Identity=31.8471337579618, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19921140, Length=231, Percent_Identity=27.7056277056277, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34945; Mature: 34945

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFHQLYILTEHDEHYREQILERQLEGLALTEDRAKATILLAAPPMAARRLDEFPRLQW
CCCCEEEEEEECCCHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCHHHHHHHHHHHHHH
LHSAYAGVDTLMDPKLRKNYLLTNVKGIFGHLIAEYVMGYAIQYQRDFRLYQTQQAERLW
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPRPYSSLANQTLVILGTGSIGSHLAHVAKQFGLRVVGVNRTGIPAKEGHFDATYHISEL
CCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEHHHH
PAALMRADLLVNTLPNTPATEGLLNQENLRHCHQALLFNVGRGKTLVEQGLPDLIAAGHI
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHH
RHAFLDVFIKEPLAQDHPFWDNPAITITPHIAAVSFPEQVVDIFADNYQRWCDNLPLRNQ
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEECCCHHHHHHHHHHHHHHHHCCCCCCCC
IDFEKGY
CCCCCCC
>Mature Secondary Structure
MNNFHQLYILTEHDEHYREQILERQLEGLALTEDRAKATILLAAPPMAARRLDEFPRLQW
CCCCEEEEEEECCCHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCHHHHHHHHHHHHHH
LHSAYAGVDTLMDPKLRKNYLLTNVKGIFGHLIAEYVMGYAIQYQRDFRLYQTQQAERLW
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPRPYSSLANQTLVILGTGSIGSHLAHVAKQFGLRVVGVNRTGIPAKEGHFDATYHISEL
CCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEHHHH
PAALMRADLLVNTLPNTPATEGLLNQENLRHCHQALLFNVGRGKTLVEQGLPDLIAAGHI
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHH
RHAFLDVFIKEPLAQDHPFWDNPAITITPHIAAVSFPEQVVDIFADNYQRWCDNLPLRNQ
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEECCCHHHHHHHHHHHHHHHHCCCCCCCC
IDFEKGY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA