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The map label for this gene is dfp

Identifier: 227080435

GI number: 227080435

Start: 208077

End: 209276

Strand: Reverse

Name: dfp

Synonym: VCM66_0203

Alternate gene names: 227080435

Gene position: 209276-208077 (Counterclockwise)

Preceding gene: 227080442

Following gene: 227080434

Centisome position: 7.24

GC content: 55.25

Gene sequence:

>1200_bases
ATGCAATCATTGGCAGGCAAAAAGATTCTGCTCGGTATCAGTGGCGGTATCGCAGCGTATAAGTGTGCAGAATTGACTCG
TCGATTGGTGGAGCGCGGTGCGACCGTGCAAGTGGTGATGACCCACGCCGCCAAAGAATTCATTACTCCGCTGACCATGC
AGGCCGTTTCCGGACGTCCCGTGTCGGATAGCTTGCTTGACCCTGCGGCAGAAGCCTCGATGGGGCATATTGAACTCGCT
AAATGGGCCGATTTGGTGCTGCTGGCCCCGGCGACTGCCGATCTCATCGCGCGCATGGCCGCAGGCATGGGCAATGATTT
GCTCACCACGCTCATTCTGGCCACCAGCGCACCAGTCGCGATTGCGCCAGCCATGAATCAGCAGATGTATCGCAATATCG
CGACCCAAGAAAATCTACAAACGCTTATCCGCCGTGGTTATCTGACTTGGGGTCCTGCGGCTGGCGAACAAGCCTGTGGA
GATGTCGGCCCAGGTCGTATGTTGGAGCCGATGGAATTAGTGGCGCACTGTGAAAACTTCTTCGCCCCGAAAATTCTTGT
CGGTAAACGCGTGCTGATCACCGCGGGCCCCACTCGTGAAGCGCTCGACCCAGTACGCTACATCACCAACCACAGCTCAG
GAAAAATGGGCTTTGCGCTCGCCAAAGCCGCCGCGCAGCTCGGCGCTGACGTCACGCTGGTCAGCGGTCCGGTGCATCTG
CCAACGCCGGTGGGCGTCAATCGCATTGATGTGCAAAGTGGATTAGAAATGCACAGCGCGGTGATGAAAGAGGCAACCTC
ACACCAGATCTTTATCGCGTGCGCCGCGGTAGCCGACTACCGCCCACAAACCGTGGCCGAACAGAAGATAAAAAAGAGCC
GTGATAACGACACATTGACCATTGAGATGGTGAAAAATCCCGATATCGTGGCGTCCGTTGCGGCTCTGACTGAGAATCGA
CCGTTTACCGTTGGCTTTGCGGCAGAAACGCAAGATGTCGAGACCTATGCGCGTAGCAAGCTCGTGCGCAAGAATCTGGA
TATGATCTGCGCTAACGACGTCTCGATTGCTGGCCAAGGCTTTAACAGCAATGACAATGCCTTGACCCTATTCTGGAAAG
AAGGGCAACACAGCCTACCTTTAACCAGTAAAGACGCGCTCGCTAGCGCAGTAATGCATTTGATTCATGAGCAAATGTAG

Upstream 100 bases:

>100_bases
TGCAGCTCGACCAGTGGCGCGATGCACAGCGGCGGTTGTGACTCCAACTTTTAGCTCGAGCTGTGCTAACATAGCGCGCA
GATTTTTTGAGGGATCCCAA

Downstream 100 bases:

>100_bases
GCGTTATTTTCGCCACCTACTTCAACCATTTTTATCAACACGCTTCGAACAACCTCTGAGCTTAGTTACACTTCAAAGGT
TTCATCTTTGTCGCAGGCGC

Product: bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase

Products: NA

Alternate protein names: DNA/pantothenate metabolism flavoprotein; Phosphopantothenoylcysteine decarboxylase; PPCDC; CoaC; Phosphopantothenate--cysteine ligase; CoaB; PPC synthetase; PPCS; Phosphopantothenoylcysteine synthase

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MQSLAGKKILLGISGGIAAYKCAELTRRLVERGATVQVVMTHAAKEFITPLTMQAVSGRPVSDSLLDPAAEASMGHIELA
KWADLVLLAPATADLIARMAAGMGNDLLTTLILATSAPVAIAPAMNQQMYRNIATQENLQTLIRRGYLTWGPAAGEQACG
DVGPGRMLEPMELVAHCENFFAPKILVGKRVLITAGPTREALDPVRYITNHSSGKMGFALAKAAAQLGADVTLVSGPVHL
PTPVGVNRIDVQSGLEMHSAVMKEATSHQIFIACAAVADYRPQTVAEQKIKKSRDNDTLTIEMVKNPDIVASVAALTENR
PFTVGFAAETQDVETYARSKLVRKNLDMICANDVSIAGQGFNSNDNALTLFWKEGQHSLPLTSKDALASAVMHLIHEQM

Sequences:

>Translated_399_residues
MQSLAGKKILLGISGGIAAYKCAELTRRLVERGATVQVVMTHAAKEFITPLTMQAVSGRPVSDSLLDPAAEASMGHIELA
KWADLVLLAPATADLIARMAAGMGNDLLTTLILATSAPVAIAPAMNQQMYRNIATQENLQTLIRRGYLTWGPAAGEQACG
DVGPGRMLEPMELVAHCENFFAPKILVGKRVLITAGPTREALDPVRYITNHSSGKMGFALAKAAAQLGADVTLVSGPVHL
PTPVGVNRIDVQSGLEMHSAVMKEATSHQIFIACAAVADYRPQTVAEQKIKKSRDNDTLTIEMVKNPDIVASVAALTENR
PFTVGFAAETQDVETYARSKLVRKNLDMICANDVSIAGQGFNSNDNALTLFWKEGQHSLPLTSKDALASAVMHLIHEQM
>Mature_399_residues
MQSLAGKKILLGISGGIAAYKCAELTRRLVERGATVQVVMTHAAKEFITPLTMQAVSGRPVSDSLLDPAAEASMGHIELA
KWADLVLLAPATADLIARMAAGMGNDLLTTLILATSAPVAIAPAMNQQMYRNIATQENLQTLIRRGYLTWGPAAGEQACG
DVGPGRMLEPMELVAHCENFFAPKILVGKRVLITAGPTREALDPVRYITNHSSGKMGFALAKAAAQLGADVTLVSGPVHL
PTPVGVNRIDVQSGLEMHSAVMKEATSHQIFIACAAVADYRPQTVAEQKIKKSRDNDTLTIEMVKNPDIVASVAALTENR
PFTVGFAAETQDVETYARSKLVRKNLDMICANDVSIAGQGFNSNDNALTLFWKEGQHSLPLTSKDALASAVMHLIHEQM

Specific function: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine

COG id: COG0452

COG function: function code H; Phosphopantothenoylcysteine synthetase/decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PPC synthetase family

Homologues:

Organism=Homo sapiens, GI71725351, Length=164, Percent_Identity=32.3170731707317, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87082301, Length=395, Percent_Identity=66.5822784810127, Blast_Score=512, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17560194, Length=175, Percent_Identity=31.4285714285714, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322762, Length=183, Percent_Identity=31.6939890710383, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322925, Length=106, Percent_Identity=33.9622641509434, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24657262, Length=187, Percent_Identity=28.8770053475936, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COABC_VIBCH (Q9KVD1)

Other databases:

- EMBL:   AE003852
- PIR:   A82351
- RefSeq:   NP_229872.1
- ProteinModelPortal:   Q9KVD1
- GeneID:   2614569
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0215
- TIGR:   VC_0215
- HOGENOM:   HBG523890
- OMA:   VTSGPTH
- ProtClustDB:   PRK05579
- BRENDA:   4.1.1.36
- BRENDA:   6.3.2.5
- InterPro:   IPR005252
- InterPro:   IPR007085
- InterPro:   IPR003382
- Gene3D:   G3DSA:3.40.50.10300
- Gene3D:   G3DSA:3.40.50.1950
- TIGRFAMs:   TIGR00521

Pfam domain/function: PF04127 DFP; PF02441 Flavoprotein; SSF102645 DNA/pantothenate-metab_flavo_C; SSF52507 Flavoprotein

EC number: =4.1.1.36; =6.3.2.5

Molecular weight: Translated: 42646; Mature: 42646

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: ACT_SITE 159-159 BINDING 126-126 BINDING 279-279 BINDING 289-289 BINDING 340-340 BINDING 344-344

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLAGKKILLGISGGIAAYKCAELTRRLVERGATVQVVMTHAAKEFITPLTMQAVSGRP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCHHHHHHHCCCC
VSDSLLDPAAEASMGHIELAKWADLVLLAPATADLIARMAAGMGNDLLTTLILATSAPVA
CCHHHCCCHHHCCCCCEEHHHHCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEE
IAPAMNQQMYRNIATQENLQTLIRRGYLTWGPAAGEQACGDVGPGRMLEPMELVAHCENF
ECHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHH
FAPKILVGKRVLITAGPTREALDPVRYITNHSSGKMGFALAKAAAQLGADVTLVSGPVHL
CCCEEEECCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCEEC
PTPVGVNRIDVQSGLEMHSAVMKEATSHQIFIACAAVADYRPQTVAEQKIKKSRDNDTLT
CCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCHHHHHHHHHHCCCCCEEE
IEMVKNPDIVASVAALTENRPFTVGFAAETQDVETYARSKLVRKNLDMICANDVSIAGQG
EEEECCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCEEECCC
FNSNDNALTLFWKEGQHSLPLTSKDALASAVMHLIHEQM
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQSLAGKKILLGISGGIAAYKCAELTRRLVERGATVQVVMTHAAKEFITPLTMQAVSGRP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCHHHHHHHCCCC
VSDSLLDPAAEASMGHIELAKWADLVLLAPATADLIARMAAGMGNDLLTTLILATSAPVA
CCHHHCCCHHHCCCCCEEHHHHCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEE
IAPAMNQQMYRNIATQENLQTLIRRGYLTWGPAAGEQACGDVGPGRMLEPMELVAHCENF
ECHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHH
FAPKILVGKRVLITAGPTREALDPVRYITNHSSGKMGFALAKAAAQLGADVTLVSGPVHL
CCCEEEECCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCEEC
PTPVGVNRIDVQSGLEMHSAVMKEATSHQIFIACAAVADYRPQTVAEQKIKKSRDNDTLT
CCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCHHHHHHHHHHCCCCCEEE
IEMVKNPDIVASVAALTENRPFTVGFAAETQDVETYARSKLVRKNLDMICANDVSIAGQG
EEEECCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCEEECCC
FNSNDNALTLFWKEGQHSLPLTSKDALASAVMHLIHEQM
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301