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The map label for this gene is thiE [H]

Identifier: 227080298

GI number: 227080298

Start: 61894

End: 63216

Strand: Direct

Name: thiE [H]

Synonym: VCM66_0062

Alternate gene names: 227080298

Gene position: 61894-63216 (Clockwise)

Preceding gene: 227080297

Following gene: 227080299

Centisome position: 2.14

GC content: 46.41

Gene sequence:

>1323_bases
ATGGTACGTTTGGTTTTTCCACGTCACCTATCAGCCTTAATCGGTCACGTGCAATACGCACTGTTGCAAGCCAAGGAACA
AGGGGTCGCTATTCAGCATATCCGTTTGGATGTGGGCTCTGAGGCTCAGTTTATTTTAGAGAAGAGTGAAGAGTCTTTGC
GAATTGGCAGTAGTTTGTGCTCTCAAAAGGAGGGTTTTGAGCCTTGCGACTACTACCTAGACTATGTGTCTGAAAACCGA
GTGTTACCCGAGGCAATGATGTGTAACGCTCGCTGCACGGTGACTGTAGGTCTTCATGATGAATATGGTTTTACGCTGGA
TAAGTGGCAGTATGGGCATGCTGCAGAGCAACTTATTGTTTATCCATCAGAGAACCATCGATTAAATAGTAAGGTAAATC
AGCATCTTGCTTGGGTTTTAGCTACGTTGACCTTAGATTTTTCGATTGGAGATGGTTTGTGCATTGCAAGAGCGGCAATC
ACTCAAGGGGATAGCGTTTCACGTGAAACATGGCCAACGCAATTCGAGCGTTTCCCTGCAGTGCAATCTAACATTCGCTC
TCTATCTACCCAAGTATTTCTAACCACCAGAGCATTTCCAACGATTGATAAAGCAAAATTTAATCTCTATCCCGTAGTGG
ATGATGTGAATTGGATTGAGCATCTTCTGAAGCTCGGTGTTAGAACGGTTCAGCTGCGAATTAAAGATCCTAAGCAGGGT
GATTTGGAAGCACAGATTATCCGAGCCATCGCGTTAGGTCGTGAATTCAACGCACAGGTTTTTATAAATGATCATTGGCA
ATTAGCCATTAAGCATCAGGCTTATGGTGTGCACCTTGGTCAAGAGGATTTGACGAGTGCGAATTTAACCGAGCTATTAG
ATGCAGGTATTCGATTGGGGCTTTCGACGCATGGTTATTATGAGTTACTGATAGCCGCAGGAATACAGCCTAGCTACATC
GCCCTTGGGCACATTTTTCCCACGACCACTAAACAGATGCCATCAAAACCACAAGGGCTAGTGCGATTGGCCGCCTATCA
GCGGTTGGTTAATCAAATGCCTTACCAAGGACAACATGGCATTCCAACGGTGGCGATTGGTGGTATTGATTGCAGAAACA
TTCGCGATGTTCTCGATTGTGGCGTCACGGCGGTTGCAGTGGTGCGGGCTATTACCGAGTCACCGGATCCTAGCCTTGCA
GTACAAGCGTTGAGTTCTGCTTTTGCCGATTTTGTAGATGCGGAGTACAAGTTGATGCCAGCCAGCGAGTCATGCGAGCC
ACTCAGTTACTTGGCTATGGAGGTAGCGGATGCTCACAGATAA

Upstream 100 bases:

>100_bases
ATCTATACAGCTGGCGATGCAAGATAAGTCTCGTGAGTTTTTAGCCTCAGGTGCTGAACTCTATCATCCTTTGGTGAAAG
AGCCGATCGAGGAGTAAGTC

Downstream 100 bases:

>100_bases
ACAGTTTCTTCGCTATCAAAGGCAGATCAGCCTTGCTGAGCTTGGAGAGGAAGGGCAGCAAAAACTGCTTAATTCACGTG
TGTTGATAGTTGGCTGCGGG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MVRLVFPRHLSALIGHVQYALLQAKEQGVAIQHIRLDVGSEAQFILEKSEESLRIGSSLCSQKEGFEPCDYYLDYVSENR
VLPEAMMCNARCTVTVGLHDEYGFTLDKWQYGHAAEQLIVYPSENHRLNSKVNQHLAWVLATLTLDFSIGDGLCIARAAI
TQGDSVSRETWPTQFERFPAVQSNIRSLSTQVFLTTRAFPTIDKAKFNLYPVVDDVNWIEHLLKLGVRTVQLRIKDPKQG
DLEAQIIRAIALGREFNAQVFINDHWQLAIKHQAYGVHLGQEDLTSANLTELLDAGIRLGLSTHGYYELLIAAGIQPSYI
ALGHIFPTTTKQMPSKPQGLVRLAAYQRLVNQMPYQGQHGIPTVAIGGIDCRNIRDVLDCGVTAVAVVRAITESPDPSLA
VQALSSAFADFVDAEYKLMPASESCEPLSYLAMEVADAHR

Sequences:

>Translated_440_residues
MVRLVFPRHLSALIGHVQYALLQAKEQGVAIQHIRLDVGSEAQFILEKSEESLRIGSSLCSQKEGFEPCDYYLDYVSENR
VLPEAMMCNARCTVTVGLHDEYGFTLDKWQYGHAAEQLIVYPSENHRLNSKVNQHLAWVLATLTLDFSIGDGLCIARAAI
TQGDSVSRETWPTQFERFPAVQSNIRSLSTQVFLTTRAFPTIDKAKFNLYPVVDDVNWIEHLLKLGVRTVQLRIKDPKQG
DLEAQIIRAIALGREFNAQVFINDHWQLAIKHQAYGVHLGQEDLTSANLTELLDAGIRLGLSTHGYYELLIAAGIQPSYI
ALGHIFPTTTKQMPSKPQGLVRLAAYQRLVNQMPYQGQHGIPTVAIGGIDCRNIRDVLDCGVTAVAVVRAITESPDPSLA
VQALSSAFADFVDAEYKLMPASESCEPLSYLAMEVADAHR
>Mature_440_residues
MVRLVFPRHLSALIGHVQYALLQAKEQGVAIQHIRLDVGSEAQFILEKSEESLRIGSSLCSQKEGFEPCDYYLDYVSENR
VLPEAMMCNARCTVTVGLHDEYGFTLDKWQYGHAAEQLIVYPSENHRLNSKVNQHLAWVLATLTLDFSIGDGLCIARAAI
TQGDSVSRETWPTQFERFPAVQSNIRSLSTQVFLTTRAFPTIDKAKFNLYPVVDDVNWIEHLLKLGVRTVQLRIKDPKQG
DLEAQIIRAIALGREFNAQVFINDHWQLAIKHQAYGVHLGQEDLTSANLTELLDAGIRLGLSTHGYYELLIAAGIQPSYI
ALGHIFPTTTKQMPSKPQGLVRLAAYQRLVNQMPYQGQHGIPTVAIGGIDCRNIRDVLDCGVTAVAVVRAITESPDPSLA
VQALSSAFADFVDAEYKLMPASESCEPLSYLAMEVADAHR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=207, Percent_Identity=54.5893719806763, Blast_Score=213, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6325042, Length=229, Percent_Identity=28.82096069869, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 48903; Mature: 48903

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLVFPRHLSALIGHVQYALLQAKEQGVAIQHIRLDVGSEAQFILEKSEESLRIGSSLC
CEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCHHHHHHHHHHH
SQKEGFEPCDYYLDYVSENRVLPEAMMCNARCTVTVGLHDEYGFTLDKWQYGHAAEQLIV
HHCCCCCHHHHHHHHHHCCCCCCHHHHCCCEEEEEEEECCCCCCEECCCCCCCCCCEEEE
YPSENHRLNSKVNQHLAWVLATLTLDFSIGDGLCIARAAITQGDSVSRETWPTQFERFPA
ECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHCHH
VQSNIRSLSTQVFLTTRAFPTIDKAKFNLYPVVDDVNWIEHLLKLGVRTVQLRIKDPKQG
HHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHCCCEEEEEEECCCCCC
DLEAQIIRAIALGREFNAQVFINDHWQLAIKHQAYGVHLGQEDLTSANLTELLDAGIRLG
CHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEEEEECCHHHCCCCHHHHHHHHHHHCC
LSTHGYYELLIAAGIQPSYIALGHIFPTTTKQMPSKPQGLVRLAAYQRLVNQMPYQGQHG
CCCCCHHEEHEECCCCCCEEEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
IPTVAIGGIDCRNIRDVLDCGVTAVAVVRAITESPDPSLAVQALSSAFADFVDAEYKLMP
CCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEC
ASESCEPLSYLAMEVADAHR
CCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVRLVFPRHLSALIGHVQYALLQAKEQGVAIQHIRLDVGSEAQFILEKSEESLRIGSSLC
CEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCHHHHHHHHHHH
SQKEGFEPCDYYLDYVSENRVLPEAMMCNARCTVTVGLHDEYGFTLDKWQYGHAAEQLIV
HHCCCCCHHHHHHHHHHCCCCCCHHHHCCCEEEEEEEECCCCCCEECCCCCCCCCCEEEE
YPSENHRLNSKVNQHLAWVLATLTLDFSIGDGLCIARAAITQGDSVSRETWPTQFERFPA
ECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHCHH
VQSNIRSLSTQVFLTTRAFPTIDKAKFNLYPVVDDVNWIEHLLKLGVRTVQLRIKDPKQG
HHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHCCCEEEEEEECCCCCC
DLEAQIIRAIALGREFNAQVFINDHWQLAIKHQAYGVHLGQEDLTSANLTELLDAGIRLG
CHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEEEEECCHHHCCCCHHHHHHHHHHHCC
LSTHGYYELLIAAGIQPSYIALGHIFPTTTKQMPSKPQGLVRLAAYQRLVNQMPYQGQHG
CCCCCHHEEHEECCCCCCEEEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
IPTVAIGGIDCRNIRDVLDCGVTAVAVVRAITESPDPSLAVQALSSAFADFVDAEYKLMP
CCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEC
ASESCEPLSYLAMEVADAHR
CCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA