Click here to switch to the map view.

The map label for this gene is thiF [H]

Identifier: 227080299

GI number: 227080299

Start: 63203

End: 63979

Strand: Direct

Name: thiF [H]

Synonym: VCM66_0063

Alternate gene names: 227080299

Gene position: 63203-63979 (Clockwise)

Preceding gene: 227080298

Following gene: 227080300

Centisome position: 2.19

GC content: 50.32

Gene sequence:

>777_bases
ATGCTCACAGATAAACAGTTTCTTCGCTATCAAAGGCAGATCAGCCTTGCTGAGCTTGGAGAGGAAGGGCAGCAAAAACT
GCTTAATTCACGTGTGTTGATAGTTGGCTGCGGGGGGCTTGGTAATGTGGTGGCCCCTTACTTGGTTGGTGCAGGTGTTG
GACAAGTGATTATCGCGGATAGTGATAGGCTGGAGTTACACAACTTACACCGTCAGATTTGCTATCACGAGGCACAAATT
GGTCACAATAAAGCCGAGCTTCTTGCTCGGTACTTGCGTGAACTCAATTCAGAAGTACGAGTGCGGGTGATTGCTCGGGA
AGTAGATGAGCTGATCCTGAACTTAGAAATCAACCAAGTGGATTTGGTGTTGGATTGTAGTGATAACCTTCCAACCCGCC
ACGCAATTAATCGAGCCTGTTATGCAGCGCAGCGGCCACTCATCAGCGGTGCCGTCATTGGGTGGGAAGGGCATCTCATG
GCGTTTGACTATCGCCAATCTACACCTTGCTATCAGTGTGTCGTACCGGATATGGCAGAGCGCCAACGCTGCAGTGACCG
AGGCGTTATTGGCCCTGTTGTCGGTATGATTGGTAACGGACAAGCCTTAATCGCACTGCATGCTCTGATGGGGAGCGCCC
ATTTTCCTGCTAACCAATTACTGCGCTTTGACGGTAAATCTATGAACTGGCAGAGCTTACAGCTTCATCCAGATAAGGTT
TGCCCAGTTTGCAGCGTATCGTCACCTGCACAACCGAAGGAGCCGCAACCGTGCTAA

Upstream 100 bases:

>100_bases
TTGAGTTCTGCTTTTGCCGATTTTGTAGATGCGGAGTACAAGTTGATGCCAGCCAGCGAGTCATGCGAGCCACTCAGTTA
CTTGGCTATGGAGGTAGCGG

Downstream 100 bases:

>100_bases
TGACTATCTACTTGAATCAGCAAGCTATCCAGACTGATATTTCTAGCTCATTATTCCATTTACATGCGCAGCTCAGCCTG
CCTAGCCAAGGTTGCGTCTT

Product: thiF protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLTDKQFLRYQRQISLAELGEEGQQKLLNSRVLIVGCGGLGNVVAPYLVGAGVGQVIIADSDRLELHNLHRQICYHEAQI
GHNKAELLARYLRELNSEVRVRVIAREVDELILNLEINQVDLVLDCSDNLPTRHAINRACYAAQRPLISGAVIGWEGHLM
AFDYRQSTPCYQCVVPDMAERQRCSDRGVIGPVVGMIGNGQALIALHALMGSAHFPANQLLRFDGKSMNWQSLQLHPDKV
CPVCSVSSPAQPKEPQPC

Sequences:

>Translated_258_residues
MLTDKQFLRYQRQISLAELGEEGQQKLLNSRVLIVGCGGLGNVVAPYLVGAGVGQVIIADSDRLELHNLHRQICYHEAQI
GHNKAELLARYLRELNSEVRVRVIAREVDELILNLEINQVDLVLDCSDNLPTRHAINRACYAAQRPLISGAVIGWEGHLM
AFDYRQSTPCYQCVVPDMAERQRCSDRGVIGPVVGMIGNGQALIALHALMGSAHFPANQLLRFDGKSMNWQSLQLHPDKV
CPVCSVSSPAQPKEPQPC
>Mature_258_residues
MLTDKQFLRYQRQISLAELGEEGQQKLLNSRVLIVGCGGLGNVVAPYLVGAGVGQVIIADSDRLELHNLHRQICYHEAQI
GHNKAELLARYLRELNSEVRVRVIAREVDELILNLEINQVDLVLDCSDNLPTRHAINRACYAAQRPLISGAVIGWEGHLM
AFDYRQSTPCYQCVVPDMAERQRCSDRGVIGPVVGMIGNGQALIALHALMGSAHFPANQLLRFDGKSMNWQSLQLHPDKV
CPVCSVSSPAQPKEPQPC

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=227, Percent_Identity=40.5286343612335, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4885649, Length=144, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI150417996, Length=152, Percent_Identity=28.9473684210526, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI87082356, Length=248, Percent_Identity=42.741935483871, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787048, Length=244, Percent_Identity=36.8852459016393, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789177, Length=135, Percent_Identity=28.8888888888889, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=256, Percent_Identity=37.890625, Blast_Score=174, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI193203301, Length=154, Percent_Identity=29.8701298701299, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI86565433, Length=159, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI86565431, Length=159, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=214, Percent_Identity=33.6448598130841, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322639, Length=161, Percent_Identity=27.9503105590062, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320598, Length=165, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24582879, Length=254, Percent_Identity=38.9763779527559, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24660640, Length=155, Percent_Identity=31.6129032258064, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28573937, Length=159, Percent_Identity=27.0440251572327, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 28552; Mature: 28552

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDKQFLRYQRQISLAELGEEGQQKLLNSRVLIVGCGGLGNVVAPYLVGAGVGQVIIAD
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCEEEEEC
SDRLELHNLHRQICYHEAQIGHNKAELLARYLRELNSEVRVRVIAREVDELILNLEINQV
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECEE
DLVLDCSDNLPTRHAINRACYAAQRPLISGAVIGWEGHLMAFDYRQSTPCYQCVVPDMAE
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCCCCCCEEECCCCHHH
RQRCSDRGVIGPVVGMIGNGQALIALHALMGSAHFPANQLLRFDGKSMNWQSLQLHPDKV
HHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHEECCCCCCCCEEEEECCCCC
CPVCSVSSPAQPKEPQPC
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTDKQFLRYQRQISLAELGEEGQQKLLNSRVLIVGCGGLGNVVAPYLVGAGVGQVIIAD
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCEEEEEC
SDRLELHNLHRQICYHEAQIGHNKAELLARYLRELNSEVRVRVIAREVDELILNLEINQV
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEEECEE
DLVLDCSDNLPTRHAINRACYAAQRPLISGAVIGWEGHLMAFDYRQSTPCYQCVVPDMAE
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCCCCCCEEECCCCHHH
RQRCSDRGVIGPVVGMIGNGQALIALHALMGSAHFPANQLLRFDGKSMNWQSLQLHPDKV
HHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHEECCCCCCCCEEEEECCCCC
CPVCSVSSPAQPKEPQPC
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]