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The map label for this gene is thiC

Identifier: 227080297

GI number: 227080297

Start: 59953

End: 61890

Strand: Direct

Name: thiC

Synonym: VCM66_0061

Alternate gene names: 227080297

Gene position: 59953-61890 (Clockwise)

Preceding gene: 227080296

Following gene: 227080298

Centisome position: 2.07

GC content: 47.57

Gene sequence:

>1938_bases
ATGTCGAACCGTAAACAAGCAAGACTGGAAGCCAAGCGCTTTATTGATACCCTTTCTGTTGAACCCTATCCTAACTCTCA
AAAATCTTACCTATTAGGCTCTCGCCCTGATATTCGGGTGCCTGTCAGAGAAATTACTCTCAGCGATACTTTGGTCGGTG
GCAGTAAAGATGCACCCATCTTTGAGCCCAATGAGCCTATCTGTGTGTATGACACATCTGGCGTCTATACTGACCCTTCA
CATGATATTGATCTCTACAAGGGGCTTCCTAAGCTCAGAGAGGAGTGGATTGAAGAGCGTCGAGATACGCACATTCTGCC
TAGTATGAGCTCTCATTTCGCCCGTGAACGCTTAGCGGATGAAACTCTAGATGAACTGCGTTATGGCCATTTACCGCGAA
TTCGCCGAGCGATGGGCCAGCATCGAGTCACTCAGTTACATTACGCACGGCAGGGAATCATTACGCCGGAAATGGAGTTT
GTGGCGATCCGTGAAAACTCTCGTCGTCTTGCTCATCAAGATCCAAGTCTACTTCAGCAGCATGCTGGGCAGAATTTTGG
TGCTCATTTACCCGATCTGATTACTCCTGAGTTTGTGCGTCGTGAGATTGCAGAAGGGCGCGCCATCATCCCATGCAATA
TTAATCATCCTGAATCTGAACCCATGATTATTGGCCGCAATTTCTTGGTTAAGGTGAATGCCAATATCGGTAACTCTTCT
GTTAGCTCTTCTATTGAAGAAGAAGTCGAGAAGTTAGTTTGGGCGACCCGCTGGGGCGCAGATACTGTGATGGATCTGTC
GACAGGGCGAAATATCCATGAAACCCGCGAGTGGATCTTACGTAATAGCCCCGTGCCAATTGGTACTGTACCTATGTATC
AGGCGCTGGAAAAAGTGAATGGTGTAGCAGAAAACCTCACATGGGAAGTGATGCGCGATACCTTACTAGAGCAAGCTGAG
CAAGGTGTGGACTATTTTACAATTCATGCGGGCTTGCTATTGCGTTATGTGCCGATGACAGCCAAGCGCGTAACGGGCAT
CGTTTCTCGTGGCGGCTCGATTATTGCGAAATGGTGTCTTTCTCACCATCAAGAGAATTTCCTTTATACCCATTTTCGCG
AAATCTGTGAGATTTGTGCGCAATATGATGTGGCTCTATCTCTAGGGGATGGACTTCGTCCTGGCTCGATTGCTGATGCT
AACGATGAAGCGCAATTTGCTGAGTTACGTACCCTTGGTGAGCTAACCCAAATAGCTTGGGAATATGATGTGCAGGTCAT
GATTGAAGGGCCGGGTCATGTACCTATGCACTTAATTAAAGCCAATATGGATGAGCAGCTTAAGCATTGTCATGAGGCGC
CATTCTATACCCTTGGTCCATTAACTACCGATATTGCCCCGGGTTATGATCACATTACCTCCGGTATCGGTGCGGCCATG
ATTGGTTGGTTTGGCTGCGCCATGCTCTGCTATGTCACACCTAAAGAACATTTGGGGCTGCCAAACAAAGAAGATGTCAA
AACAGGATTGATTACTTATAAGTTAGCGGCTCATGCAGCAGATTTGGCGAAAGGGCATCCGGGAGCGCAAATTCGTGATA
ATGCATTATCAAAAGCACGTTTTGAGTTTCGTTGGGAAGACCAGTTCAATCTTGCGCTTGATCCCGTCACAGCACGCGCT
TTCCATGATGAGACCCTACCGCAGGAATCGGGCAAAGTCGCGCACTTTTGCTCTATGTGTGGCCCCAAATTCTGCTCGAT
GAAGATCTCGCAAGAGGTCAGGGATTACGCAAATAACCAGACATTAGACACCACCGTCATTGACTTGGTTATGCCTGCAG
AATCTATACAGCTGGCGATGCAAGATAAGTCTCGTGAGTTTTTAGCCTCAGGTGCTGAACTCTATCATCCTTTGGTGAAA
GAGCCGATCGAGGAGTAA

Upstream 100 bases:

>100_bases
TAGGTGACCTATTGTTATTAATAGCGTACTTACCTGTCGACTCATCTCTCATAGCATCTTTCCGTCAAGCATTTCTCCCT
CTTATTAAAAAGGAAATGCT

Downstream 100 bases:

>100_bases
GTCATGGTACGTTTGGTTTTTCCACGTCACCTATCAGCCTTAATCGGTCACGTGCAATACGCACTGTTGCAAGCCAAGGA
ACAAGGGGTCGCTATTCAGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MSNRKQARLEAKRFIDTLSVEPYPNSQKSYLLGSRPDIRVPVREITLSDTLVGGSKDAPIFEPNEPICVYDTSGVYTDPS
HDIDLYKGLPKLREEWIEERRDTHILPSMSSHFARERLADETLDELRYGHLPRIRRAMGQHRVTQLHYARQGIITPEMEF
VAIRENSRRLAHQDPSLLQQHAGQNFGAHLPDLITPEFVRREIAEGRAIIPCNINHPESEPMIIGRNFLVKVNANIGNSS
VSSSIEEEVEKLVWATRWGADTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAENLTWEVMRDTLLEQAE
QGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCLSHHQENFLYTHFREICEICAQYDVALSLGDGLRPGSIADA
NDEAQFAELRTLGELTQIAWEYDVQVMIEGPGHVPMHLIKANMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
IGWFGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLALDPVTARA
FHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYANNQTLDTTVIDLVMPAESIQLAMQDKSREFLASGAELYHPLVK
EPIEE

Sequences:

>Translated_645_residues
MSNRKQARLEAKRFIDTLSVEPYPNSQKSYLLGSRPDIRVPVREITLSDTLVGGSKDAPIFEPNEPICVYDTSGVYTDPS
HDIDLYKGLPKLREEWIEERRDTHILPSMSSHFARERLADETLDELRYGHLPRIRRAMGQHRVTQLHYARQGIITPEMEF
VAIRENSRRLAHQDPSLLQQHAGQNFGAHLPDLITPEFVRREIAEGRAIIPCNINHPESEPMIIGRNFLVKVNANIGNSS
VSSSIEEEVEKLVWATRWGADTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAENLTWEVMRDTLLEQAE
QGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCLSHHQENFLYTHFREICEICAQYDVALSLGDGLRPGSIADA
NDEAQFAELRTLGELTQIAWEYDVQVMIEGPGHVPMHLIKANMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
IGWFGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLALDPVTARA
FHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYANNQTLDTTVIDLVMPAESIQLAMQDKSREFLASGAELYHPLVK
EPIEE
>Mature_644_residues
SNRKQARLEAKRFIDTLSVEPYPNSQKSYLLGSRPDIRVPVREITLSDTLVGGSKDAPIFEPNEPICVYDTSGVYTDPSH
DIDLYKGLPKLREEWIEERRDTHILPSMSSHFARERLADETLDELRYGHLPRIRRAMGQHRVTQLHYARQGIITPEMEFV
AIRENSRRLAHQDPSLLQQHAGQNFGAHLPDLITPEFVRREIAEGRAIIPCNINHPESEPMIIGRNFLVKVNANIGNSSV
SSSIEEEVEKLVWATRWGADTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAENLTWEVMRDTLLEQAEQ
GVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCLSHHQENFLYTHFREICEICAQYDVALSLGDGLRPGSIADAN
DEAQFAELRTLGELTQIAWEYDVQVMIEGPGHVPMHLIKANMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMI
GWFGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLALDPVTARAF
HDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYANNQTLDTTVIDLVMPAESIQLAMQDKSREFLASGAELYHPLVKE
PIEE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=633, Percent_Identity=75.9873617693523, Blast_Score=1003, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_VIBC3 (A5F4D2)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- STRING:   A5F4D2
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2452
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   VCHO345073:VC0395_A2452-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 72634; Mature: 72503

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: BINDING 235-235 BINDING 264-264 BINDING 293-293 BINDING 329-329 BINDING 429-429 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRKQARLEAKRFIDTLSVEPYPNSQKSYLLGSRPDIRVPVREITLSDTLVGGSKDAPI
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHEEEHHHCCCCCCCCCC
FEPNEPICVYDTSGVYTDPSHDIDLYKGLPKLREEWIEERRDTHILPSMSSHFARERLAD
CCCCCCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ETLDELRYGHLPRIRRAMGQHRVTQLHYARQGIITPEMEFVAIRENSRRLAHQDPSLLQQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCHHHHHH
HAGQNFGAHLPDLITPEFVRREIAEGRAIIPCNINHPESEPMIIGRNFLVKVNANIGNSS
HCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEECCEEEEEEECCCCCHH
VSSSIEEEVEKLVWATRWGADTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVN
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVAENLTWEVMRDTLLEQAEQGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SHHQENFLYTHFREICEICAQYDVALSLGDGLRPGSIADANDEAQFAELRTLGELTQIAW
HHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EYDVQVMIEGPGHVPMHLIKANMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
EECEEEEEECCCCCCHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
IGWFGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKAR
HHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCE
FEFRWEDQFNLALDPVTARAFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYANNQ
EEEEECCCCCEEECCHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCC
TLDTTVIDLVMPAESIQLAMQDKSREFLASGAELYHPLVKEPIEE
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNRKQARLEAKRFIDTLSVEPYPNSQKSYLLGSRPDIRVPVREITLSDTLVGGSKDAPI
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHEEEHHHCCCCCCCCCC
FEPNEPICVYDTSGVYTDPSHDIDLYKGLPKLREEWIEERRDTHILPSMSSHFARERLAD
CCCCCCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ETLDELRYGHLPRIRRAMGQHRVTQLHYARQGIITPEMEFVAIRENSRRLAHQDPSLLQQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCHHHHHH
HAGQNFGAHLPDLITPEFVRREIAEGRAIIPCNINHPESEPMIIGRNFLVKVNANIGNSS
HCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEECCEEEEEEECCCCCHH
VSSSIEEEVEKLVWATRWGADTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVN
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GVAENLTWEVMRDTLLEQAEQGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SHHQENFLYTHFREICEICAQYDVALSLGDGLRPGSIADANDEAQFAELRTLGELTQIAW
HHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EYDVQVMIEGPGHVPMHLIKANMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
EECEEEEEECCCCCCHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
IGWFGCAMLCYVTPKEHLGLPNKEDVKTGLITYKLAAHAADLAKGHPGAQIRDNALSKAR
HHHHHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCE
FEFRWEDQFNLALDPVTARAFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYANNQ
EEEEECCCCCEEECCHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCC
TLDTTVIDLVMPAESIQLAMQDKSREFLASGAELYHPLVKEPIEE
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA