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The map label for this gene is hemF [H]

Identifier: 227080292

GI number: 227080292

Start: 50688

End: 51605

Strand: Direct

Name: hemF [H]

Synonym: VCM66_0055

Alternate gene names: 227080292

Gene position: 50688-51605 (Clockwise)

Preceding gene: 227080291

Following gene: 227080293

Centisome position: 1.75

GC content: 49.13

Gene sequence:

>918_bases
GTGAAGTCAAACATAGATAAGCAAGCAGTGAAGAACTTTTTGCTGCAGTTACAAGATCAAATTTGCCAGCAATTGGAAGC
GGCGGATGGTCAAGCGCAATTTATTGAAGATGCATGGCAACGTGAGCCGGGCGAAAAATTGGGTGGTGGTGGCCGTACCC
GAGTGATGCGAGATGGCGCCGTTTTCGAACAAGGCGGTGTGAACTTCTCCCATGTATTTGGTGAACAGATGCCCGGCTCT
GCTACTGCCCATCGTCCAGAATTGGCCGGTCGTCGCTTTGAAGCCATGGGTGTTTCTCTGGTCATGCATCCGAAAAACCC
GTATGTCCCGACCTCTCATGCCAATGTGCGCTATTTTATTGCTGAAAAAGAGGGGGAAGATCCGATCTGGTGGTTTGGTG
GTGGTTTTGACCTTACGCCGTTTTACCCCTTTGTTGAGGATTGCCAGCTCTGGCACCAAACTGCGAAAAATCTCTGCGCA
CCTTTTGGGGCAGAAATCTATAACGAGCATAAAGCATGGTGTGATCGCTACTTCTATTTACCGCACCGTAATGAAACACG
TGGTATCGGCGGATTATTTTTTGATGATCTCAATGAATGGCCATTCGAGCAATGTTTTGCCTATATGCAGGCGGTCGGTG
AAGGTTATACCCAAGCTTATGTGCCGATTGTCGAAAAACGCAAAAACACGCCCTTTACAGAGCGCGAGCGCCAATTCCAG
CTCTATCGTCGTGGTCGATATGTTGAATTTAATTTGGTGTTGGATCGTGGCACGCTGTTTGGTCTGCAAACCGGTGGTCG
TACCGAGTCAATTTTGATGTCGATGCCGCCTCTAGCACGTTGGGAATACGCTTATCAACCTGAGTCCGGTACCCCAGAAG
CCCAGTTGAGCGAGTTTTTAGTTCCGCGTGAATGGTGA

Upstream 100 bases:

>100_bases
AGTAGAAGGGAAGACCAGTGGCCGTTGTGGCCCGAGTGAAATTCGCGATGCACGCAGTTTACAAGTGTTAAGACAGGGAT
AACCCTGCGCAGGAGAAAAC

Downstream 100 bases:

>100_bases
CACTTGAAGTTCTGAGTGATAGGGTCGCGCAAGTGACCCTTTTGATTGTTTAGGCAAGGATATGGCTTCTCAAATCGATC
AGTATGCCGTTTTTGGTAAC

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKSNIDKQAVKNFLLQLQDQICQQLEAADGQAQFIEDAWQREPGEKLGGGGRTRVMRDGAVFEQGGVNFSHVFGEQMPGS
ATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRYFIAEKEGEDPIWWFGGGFDLTPFYPFVEDCQLWHQTAKNLCA
PFGAEIYNEHKAWCDRYFYLPHRNETRGIGGLFFDDLNEWPFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPESGTPEAQLSEFLVPREW

Sequences:

>Translated_305_residues
MKSNIDKQAVKNFLLQLQDQICQQLEAADGQAQFIEDAWQREPGEKLGGGGRTRVMRDGAVFEQGGVNFSHVFGEQMPGS
ATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRYFIAEKEGEDPIWWFGGGFDLTPFYPFVEDCQLWHQTAKNLCA
PFGAEIYNEHKAWCDRYFYLPHRNETRGIGGLFFDDLNEWPFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPESGTPEAQLSEFLVPREW
>Mature_305_residues
MKSNIDKQAVKNFLLQLQDQICQQLEAADGQAQFIEDAWQREPGEKLGGGGRTRVMRDGAVFEQGGVNFSHVFGEQMPGS
ATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRYFIAEKEGEDPIWWFGGGFDLTPFYPFVEDCQLWHQTAKNLCA
PFGAEIYNEHKAWCDRYFYLPHRNETRGIGGLFFDDLNEWPFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPESGTPEAQLSEFLVPREW

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=308, Percent_Identity=45.7792207792208, Blast_Score=265, Evalue=6e-71,
Organism=Escherichia coli, GI1788777, Length=300, Percent_Identity=74, Blast_Score=464, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6320249, Length=318, Percent_Identity=43.3962264150943, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI17864388, Length=302, Percent_Identity=46.3576158940397, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 35152; Mature: 35152

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNIDKQAVKNFLLQLQDQICQQLEAADGQAQFIEDAWQREPGEKLGGGGRTRVMRDGA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCEEEEECCC
VFEQGGVNFSHVFGEQMPGSATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRYFI
EEECCCCCHHHHHCCCCCCCCCCCCCHHHCCEEHCCCEEEEECCCCCCCCCCCCCEEEEE
AEKEGEDPIWWFGGGFDLTPFYPFVEDCQLWHQTAKNLCAPFGAEIYNEHKAWCDRYFYL
EECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEC
PHRNETRGIGGLFFDDLNEWPFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPESGTPEAQLSEFL
HHHCCCEEEEEEEEECCEEEEECCCCCCHHHEECCCCCHHCEEEECCCCCCCHHHHHHHC
VPREW
CCCCC
>Mature Secondary Structure
MKSNIDKQAVKNFLLQLQDQICQQLEAADGQAQFIEDAWQREPGEKLGGGGRTRVMRDGA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCEEEEECCC
VFEQGGVNFSHVFGEQMPGSATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRYFI
EEECCCCCHHHHHCCCCCCCCCCCCCHHHCCEEHCCCEEEEECCCCCCCCCCCCCEEEEE
AEKEGEDPIWWFGGGFDLTPFYPFVEDCQLWHQTAKNLCAPFGAEIYNEHKAWCDRYFYL
EECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEC
PHRNETRGIGGLFFDDLNEWPFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPESGTPEAQLSEFL
HHHCCCEEEEEEEEECCEEEEECCCCCCHHHEECCCCCHHCEEEECCCCCCCHHHHHHHC
VPREW
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA