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The map label for this gene is rimN [H]

Identifier: 227080291

GI number: 227080291

Start: 50103

End: 50669

Strand: Direct

Name: rimN [H]

Synonym: VCM66_0054

Alternate gene names: 227080291

Gene position: 50103-50669 (Clockwise)

Preceding gene: 227080290

Following gene: 227080292

Centisome position: 1.73

GC content: 52.91

Gene sequence:

>567_bases
GTGGCTCTCGTGGAGAATCTACAACAGGCGGTGGATGCGCTACGCAAAGGTTGCGTTATTGCCTATCCAACCGAAGGGGT
GTTTGGTTTGGGGTGTGATCCGGATAACCAAACCGCTATGCAGCGCTTATTGGCAATCAAGCAGCGTCCCGTGGAAAAAG
GGGTGATCTTAATTGCCGCTAGCTATGCGCAGTTGCGCCCTTATGTCGATGAAACGCAATTAACCGCAGATCAACTGGCA
CAAGTGCTGGCTTCATGGCCCGCGCCACTGACTTGGGTCATGCCAGCCAGCGGTGATACGCCAAGCTGGGTTCGTGGTCA
GTTTGATACCGTTGCCGTGCGTGTTTCGGATCATCCGGTCGTTCAGAAGTTATGTCTCGCCTTTGGGAAGCCACTGACGT
CAACCAGTGCTAATCTAAGTGGCCAGCCCGCGTGCGTAACTCAACAAGAAGTCATGGTTCAGCTAGGCAATCAGATTGCT
GTTGTAGTAGAAGGGAAGACCAGTGGCCGTTGTGGCCCGAGTGAAATTCGCGATGCACGCAGTTTACAAGTGTTAAGACA
GGGATAA

Upstream 100 bases:

>100_bases
CGGGGATTTTTTTCTCAAGCAAACGTTTGCTTTTTCTGGTTGATGAGAAGAACTGGTCAATTGCTCCACGATTTGTACAC
TGAGCACAAATGAAAGAGGA

Downstream 100 bases:

>100_bases
CCCTGCGCAGGAGAAAACGTGAAGTCAAACATAGATAAGCAAGCAGTGAAGAACTTTTTGCTGCAGTTACAAGATCAAAT
TTGCCAGCAATTGGAAGCGG

Product: Sua5/YciO/YrdC family protein

Products: NA

Alternate protein names: t(6)A37 threonylcarbamoyladenosine biosynthesis protein RimN [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMQRLLAIKQRPVEKGVILIAASYAQLRPYVDETQLTADQLA
QVLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPVVQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIA
VVVEGKTSGRCGPSEIRDARSLQVLRQG

Sequences:

>Translated_188_residues
MALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMQRLLAIKQRPVEKGVILIAASYAQLRPYVDETQLTADQLA
QVLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPVVQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIA
VVVEGKTSGRCGPSEIRDARSLQVLRQG
>Mature_187_residues
ALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMQRLLAIKQRPVEKGVILIAASYAQLRPYVDETQLTADQLAQ
VLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPVVQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIAV
VVEGKTSGRCGPSEIRDARSLQVLRQG

Specific function: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine [H]

COG id: COG0009

COG function: function code J; Putative translation factor (SUA5)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367210, Length=189, Percent_Identity=56.6137566137566, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI87081864, Length=168, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17537239, Length=170, Percent_Identity=30.5882352941176, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78707274, Length=173, Percent_Identity=32.9479768786127, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR004388
- InterPro:   IPR006070 [H]

Pfam domain/function: PF01300 Sua5_yciO_yrdC [H]

EC number: NA

Molecular weight: Translated: 20184; Mature: 20053

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMQRLLAIKQRPVEKGVILIAA
CHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
SYAQLRPYVDETQLTADQLAQVLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPV
CHHHHCCCCCHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCEEEEEEECCCHH
VQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIAVVVEGKTSGRCGPSEIRDAR
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
SLQVLRQG
HHHHHHCC
>Mature Secondary Structure 
ALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMQRLLAIKQRPVEKGVILIAA
HHHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
SYAQLRPYVDETQLTADQLAQVLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPV
CHHHHCCCCCHHHCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCEEEEEEECCCHH
VQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIAVVVEGKTSGRCGPSEIRDAR
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
SLQVLRQG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA